Experiment: Bas69
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt xis and OKFHMN_00775 are separated by 103 nucleotides OKFHMN_00775 and OKFHMN_00780 overlap by 4 nucleotides OKFHMN_00780 and renD overlap by 4 nucleotides renD and emrE are separated by 67 nucleotides emrE and ybcK are separated by 244 nucleotides
OKFHMN_00770: xis - Excisionase, at 55,794 to 56,030
xis
OKFHMN_00775: OKFHMN_00775 - replication protein, at 56,134 to 56,958
_00775
OKFHMN_00780: OKFHMN_00780 - Replication protein P, at 56,955 to 57,656
_00780
OKFHMN_00785: renD - Putative protein RenD, at 57,653 to 57,955
renD
OKFHMN_00790: emrE - multidrug efflux SMR transporter EmrE, at 58,023 to 58,355
emrE
OKFHMN_00795: ybcK - Uncharacterized protein YbcK, at 58,600 to 60,126
ybcK
Position (kb) 56
57
58 Strain fitness (log2 ratio) -2
-1
0
1 at 56.101 kb on + strand at 56.522 kb on + strand, within OKFHMN_00775 at 56.897 kb on + strand at 57.339 kb on - strand, within OKFHMN_00780 at 57.521 kb on + strand, within OKFHMN_00780 at 57.626 kb on + strand at 57.653 kb on - strand at 57.694 kb on + strand, within renD at 57.750 kb on + strand, within renD at 58.181 kb on + strand, within emrE
Per-strain Table
Position Strand Gene LocusTag Fraction Bas69 remove 56,101 + +0.4 56,522 + OKFHMN_00775 0.47 -1.5 56,897 + -1.8 57,339 - OKFHMN_00780 0.55 +0.2 57,521 + OKFHMN_00780 0.81 +0.1 57,626 + +1.4 57,653 - -0.6 57,694 + renD OKFHMN_00785 0.14 +0.1 57,750 + renD OKFHMN_00785 0.32 -1.2 58,181 + emrE OKFHMN_00790 0.47 +0.7
Or see this region's nucleotide sequence