Strain Fitness in Escherichia coli ECOR27 around NOLOHH_23025

Experiment: Control_ECOR27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntea10 and NOLOHH_23005 are separated by 75 nucleotidesNOLOHH_23005 and NOLOHH_23010 overlap by 4 nucleotidesNOLOHH_23010 and NOLOHH_23015 overlap by 4 nucleotidesNOLOHH_23015 and NOLOHH_23020 overlap by 20 nucleotidesNOLOHH_23020 and NOLOHH_23025 overlap by 4 nucleotidesNOLOHH_23025 and NOLOHH_23030 overlap by 17 nucleotidesNOLOHH_23030 and NOLOHH_23035 are separated by 342 nucleotides NOLOHH_23000: ea10 - Protein ea10, at 4,529,969 to 4,530,337 ea10 NOLOHH_23005: NOLOHH_23005 - Regulatory protein CIII, at 4,530,413 to 4,530,583 _23005 NOLOHH_23010: NOLOHH_23010 - hypothetical protein, at 4,530,580 to 4,530,744 _23010 NOLOHH_23015: NOLOHH_23015 - hypothetical protein, at 4,530,741 to 4,530,848 _23015 NOLOHH_23020: NOLOHH_23020 - hypothetical protein, at 4,530,829 to 4,531,137 _23020 NOLOHH_23025: NOLOHH_23025 - DNA recombinase, at 4,531,134 to 4,532,036 _23025 NOLOHH_23030: NOLOHH_23030 - siphovirus Gp157 family protein, at 4,532,020 to 4,532,502 _23030 NOLOHH_23035: NOLOHH_23035 - DUF5405 domain-containing protein, at 4,532,845 to 4,533,126 _23035 Position (kb) 4531 4532 4533Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4530.183 kb on + strand, within ea10at 4530.192 kb on - strand, within ea10at 4530.573 kb on + strandat 4530.693 kb on - strand, within NOLOHH_23010at 4530.850 kb on - strandat 4530.987 kb on + strand, within NOLOHH_23020at 4530.988 kb on - strand, within NOLOHH_23020at 4531.087 kb on + strand, within NOLOHH_23020at 4531.087 kb on + strand, within NOLOHH_23020at 4531.087 kb on + strand, within NOLOHH_23020at 4531.265 kb on + strand, within NOLOHH_23025at 4531.359 kb on + strand, within NOLOHH_23025at 4531.359 kb on + strand, within NOLOHH_23025at 4531.359 kb on + strand, within NOLOHH_23025at 4531.401 kb on + strand, within NOLOHH_23025at 4531.401 kb on + strand, within NOLOHH_23025at 4531.402 kb on - strand, within NOLOHH_23025at 4531.402 kb on - strand, within NOLOHH_23025at 4531.403 kb on + strand, within NOLOHH_23025at 4531.404 kb on - strand, within NOLOHH_23025at 4531.411 kb on + strand, within NOLOHH_23025at 4531.411 kb on + strand, within NOLOHH_23025at 4531.443 kb on - strand, within NOLOHH_23025at 4531.482 kb on + strand, within NOLOHH_23025at 4531.483 kb on - strand, within NOLOHH_23025at 4531.483 kb on - strand, within NOLOHH_23025at 4531.483 kb on - strand, within NOLOHH_23025at 4531.483 kb on - strand, within NOLOHH_23025at 4531.483 kb on - strand, within NOLOHH_23025at 4531.522 kb on - strand, within NOLOHH_23025at 4531.720 kb on - strand, within NOLOHH_23025at 4531.720 kb on - strand, within NOLOHH_23025at 4531.778 kb on + strand, within NOLOHH_23025at 4531.821 kb on - strand, within NOLOHH_23025at 4531.991 kb on - strandat 4532.075 kb on + strand, within NOLOHH_23030at 4532.514 kb on - strandat 4533.005 kb on + strand, within NOLOHH_23035at 4533.006 kb on - strand, within NOLOHH_23035

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECOR27
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4,530,183 + ea10 NOLOHH_23000 0.58 +2.3
4,530,192 - ea10 NOLOHH_23000 0.60 -1.3
4,530,573 + +0.1
4,530,693 - NOLOHH_23010 0.68 +1.7
4,530,850 - -0.3
4,530,987 + NOLOHH_23020 0.51 -0.4
4,530,988 - NOLOHH_23020 0.51 -0.3
4,531,087 + NOLOHH_23020 0.83 +0.4
4,531,087 + NOLOHH_23020 0.83 +3.3
4,531,087 + NOLOHH_23020 0.83 +1.7
4,531,265 + NOLOHH_23025 0.15 +1.3
4,531,359 + NOLOHH_23025 0.25 -1.3
4,531,359 + NOLOHH_23025 0.25 +0.5
4,531,359 + NOLOHH_23025 0.25 +1.0
4,531,401 + NOLOHH_23025 0.30 -1.5
4,531,401 + NOLOHH_23025 0.30 -1.3
4,531,402 - NOLOHH_23025 0.30 -1.3
4,531,402 - NOLOHH_23025 0.30 -0.9
4,531,403 + NOLOHH_23025 0.30 +1.0
4,531,404 - NOLOHH_23025 0.30 -0.9
4,531,411 + NOLOHH_23025 0.31 -2.5
4,531,411 + NOLOHH_23025 0.31 +0.1
4,531,443 - NOLOHH_23025 0.34 -1.0
4,531,482 + NOLOHH_23025 0.39 +0.9
4,531,483 - NOLOHH_23025 0.39 -0.5
4,531,483 - NOLOHH_23025 0.39 -0.5
4,531,483 - NOLOHH_23025 0.39 -0.9
4,531,483 - NOLOHH_23025 0.39 -0.3
4,531,483 - NOLOHH_23025 0.39 -0.6
4,531,522 - NOLOHH_23025 0.43 -1.6
4,531,720 - NOLOHH_23025 0.65 -1.6
4,531,720 - NOLOHH_23025 0.65 -0.9
4,531,778 + NOLOHH_23025 0.71 -1.5
4,531,821 - NOLOHH_23025 0.76 +0.7
4,531,991 - -0.6
4,532,075 + NOLOHH_23030 0.11 -0.2
4,532,514 - -1.6
4,533,005 + NOLOHH_23035 0.57 +1.0
4,533,006 - NOLOHH_23035 0.57 -1.1

Or see this region's nucleotide sequence