Strain Fitness in Escherichia coli ECOR27 around NOLOHH_21850

Experiment: Control_ECOR27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycbX and zapC overlap by 4 nucleotideszapC and pyrD are separated by 173 nucleotidespyrD and NOLOHH_21855 are separated by 110 nucleotidesNOLOHH_21855 and fimA are separated by 13 nucleotides NOLOHH_21840: ycbX - 6-N-hydroxylaminopurine resistance protein YcbX, at 4,295,206 to 4,296,315 ycbX NOLOHH_21845: zapC - cell division protein ZapC, at 4,296,312 to 4,296,854 zapC NOLOHH_21850: pyrD - quinone-dependent dihydroorotate dehydrogenase, at 4,297,028 to 4,298,038 pyrD NOLOHH_21855: NOLOHH_21855 - Uncharacterized fimbrial chaperone YcbF, at 4,298,149 to 4,298,838 _21855 NOLOHH_21860: fimA - fimbrial protein, at 4,298,852 to 4,299,415 fimA Position (kb) 4297 4298 4299Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4296.077 kb on + strand, within ycbXat 4296.077 kb on + strand, within ycbXat 4296.166 kb on + strand, within ycbXat 4296.300 kb on + strandat 4296.300 kb on + strandat 4296.301 kb on - strandat 4296.348 kb on - strandat 4296.380 kb on + strand, within zapCat 4296.645 kb on - strand, within zapCat 4296.654 kb on - strand, within zapCat 4296.769 kb on - strand, within zapCat 4296.784 kb on - strand, within zapCat 4296.913 kb on + strandat 4296.947 kb on - strandat 4297.026 kb on - strandat 4297.030 kb on - strandat 4297.241 kb on + strand, within pyrDat 4297.241 kb on + strand, within pyrDat 4297.342 kb on + strand, within pyrDat 4297.408 kb on - strand, within pyrDat 4297.408 kb on - strand, within pyrDat 4297.408 kb on - strand, within pyrDat 4297.483 kb on - strand, within pyrDat 4297.610 kb on - strand, within pyrDat 4297.625 kb on - strand, within pyrDat 4297.691 kb on - strand, within pyrDat 4297.691 kb on - strand, within pyrDat 4297.728 kb on - strand, within pyrDat 4297.952 kb on - strandat 4297.962 kb on - strandat 4298.079 kb on + strandat 4298.283 kb on + strand, within NOLOHH_21855at 4298.283 kb on + strand, within NOLOHH_21855at 4298.283 kb on + strand, within NOLOHH_21855at 4298.283 kb on + strand, within NOLOHH_21855at 4298.284 kb on - strand, within NOLOHH_21855at 4298.284 kb on - strand, within NOLOHH_21855at 4298.296 kb on - strand, within NOLOHH_21855at 4298.296 kb on - strand, within NOLOHH_21855at 4298.302 kb on + strand, within NOLOHH_21855at 4298.302 kb on + strand, within NOLOHH_21855at 4298.302 kb on + strand, within NOLOHH_21855at 4298.303 kb on - strand, within NOLOHH_21855at 4298.327 kb on + strand, within NOLOHH_21855at 4298.327 kb on + strand, within NOLOHH_21855at 4298.328 kb on - strand, within NOLOHH_21855at 4298.330 kb on + strand, within NOLOHH_21855at 4298.330 kb on + strand, within NOLOHH_21855at 4298.381 kb on - strand, within NOLOHH_21855at 4298.381 kb on - strand, within NOLOHH_21855at 4298.464 kb on + strand, within NOLOHH_21855at 4298.465 kb on - strand, within NOLOHH_21855at 4298.510 kb on - strand, within NOLOHH_21855at 4298.581 kb on - strand, within NOLOHH_21855at 4298.581 kb on - strand, within NOLOHH_21855at 4298.686 kb on - strand, within NOLOHH_21855at 4298.689 kb on - strand, within NOLOHH_21855at 4298.710 kb on - strand, within NOLOHH_21855at 4298.712 kb on + strand, within NOLOHH_21855at 4298.720 kb on - strand, within NOLOHH_21855at 4298.754 kb on + strand, within NOLOHH_21855at 4298.854 kb on - strandat 4298.960 kb on - strand, within fimAat 4298.963 kb on + strand, within fimAat 4298.963 kb on + strand, within fimAat 4298.964 kb on - strand, within fimAat 4298.964 kb on - strand, within fimAat 4298.970 kb on + strand, within fimA

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECOR27
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4,296,077 + ycbX NOLOHH_21840 0.78 +3.2
4,296,077 + ycbX NOLOHH_21840 0.78 +0.1
4,296,166 + ycbX NOLOHH_21840 0.86 +0.0
4,296,300 + +0.9
4,296,300 + -0.7
4,296,301 - -0.4
4,296,348 - -0.4
4,296,380 + zapC NOLOHH_21845 0.13 +1.6
4,296,645 - zapC NOLOHH_21845 0.61 +0.3
4,296,654 - zapC NOLOHH_21845 0.63 +1.2
4,296,769 - zapC NOLOHH_21845 0.84 +2.6
4,296,784 - zapC NOLOHH_21845 0.87 +0.0
4,296,913 + +1.0
4,296,947 - +0.2
4,297,026 - +0.3
4,297,030 - +0.4
4,297,241 + pyrD NOLOHH_21850 0.21 -1.4
4,297,241 + pyrD NOLOHH_21850 0.21 -0.4
4,297,342 + pyrD NOLOHH_21850 0.31 -1.0
4,297,408 - pyrD NOLOHH_21850 0.38 +1.6
4,297,408 - pyrD NOLOHH_21850 0.38 -0.4
4,297,408 - pyrD NOLOHH_21850 0.38 -0.4
4,297,483 - pyrD NOLOHH_21850 0.45 +0.6
4,297,610 - pyrD NOLOHH_21850 0.58 -2.4
4,297,625 - pyrD NOLOHH_21850 0.59 +0.6
4,297,691 - pyrD NOLOHH_21850 0.66 -2.4
4,297,691 - pyrD NOLOHH_21850 0.66 -0.4
4,297,728 - pyrD NOLOHH_21850 0.69 -0.4
4,297,952 - +0.6
4,297,962 - -0.2
4,298,079 + +1.2
4,298,283 + NOLOHH_21855 0.19 -0.7
4,298,283 + NOLOHH_21855 0.19 -0.2
4,298,283 + NOLOHH_21855 0.19 +0.2
4,298,283 + NOLOHH_21855 0.19 -1.0
4,298,284 - NOLOHH_21855 0.20 +0.6
4,298,284 - NOLOHH_21855 0.20 +1.1
4,298,296 - NOLOHH_21855 0.21 -0.4
4,298,296 - NOLOHH_21855 0.21 -0.4
4,298,302 + NOLOHH_21855 0.22 +2.2
4,298,302 + NOLOHH_21855 0.22 +0.6
4,298,302 + NOLOHH_21855 0.22 +0.6
4,298,303 - NOLOHH_21855 0.22 +0.6
4,298,327 + NOLOHH_21855 0.26 -1.0
4,298,327 + NOLOHH_21855 0.26 +0.6
4,298,328 - NOLOHH_21855 0.26 -1.0
4,298,330 + NOLOHH_21855 0.26 -0.1
4,298,330 + NOLOHH_21855 0.26 -1.2
4,298,381 - NOLOHH_21855 0.34 +0.2
4,298,381 - NOLOHH_21855 0.34 +0.9
4,298,464 + NOLOHH_21855 0.46 -0.7
4,298,465 - NOLOHH_21855 0.46 +0.6
4,298,510 - NOLOHH_21855 0.52 -1.7
4,298,581 - NOLOHH_21855 0.63 -0.4
4,298,581 - NOLOHH_21855 0.63 -0.7
4,298,686 - NOLOHH_21855 0.78 -0.4
4,298,689 - NOLOHH_21855 0.78 -0.8
4,298,710 - NOLOHH_21855 0.81 -0.4
4,298,712 + NOLOHH_21855 0.82 +2.0
4,298,720 - NOLOHH_21855 0.83 +0.6
4,298,754 + NOLOHH_21855 0.88 -0.4
4,298,854 - +0.6
4,298,960 - fimA NOLOHH_21860 0.19 +1.4
4,298,963 + fimA NOLOHH_21860 0.20 -1.4
4,298,963 + fimA NOLOHH_21860 0.20 +0.8
4,298,964 - fimA NOLOHH_21860 0.20 +3.0
4,298,964 - fimA NOLOHH_21860 0.20 +0.6
4,298,970 + fimA NOLOHH_21860 0.21 -0.1

Or see this region's nucleotide sequence