Experiment: Control_ECOR27
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdsA and sirB1 are separated by 35 nucleotides sirB1 and sirB2 are separated by 3 nucleotides sirB2 and prmC overlap by 4 nucleotides prmC and prfA overlap by 1 nucleotides
NOLOHH_20590: kdsA - 3-deoxy-8-phosphooctulonate synthase, at 4,050,056 to 4,050,910
kdsA
NOLOHH_20595: sirB1 - invasion regulator SirB1, at 4,050,946 to 4,051,755
sirB1
NOLOHH_20600: sirB2 - invasion regulator SirB2, at 4,051,759 to 4,052,151
sirB2
NOLOHH_20605: prmC - peptide chain release factor N(5)-glutamine methyltransferase, at 4,052,148 to 4,052,981
prmC
NOLOHH_20610: prfA - peptide chain release factor 1, at 4,052,981 to 4,054,063
prfA
Position (kb) 4051
4052
4053 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4050.948 kb on - strand at 4050.967 kb on - strand at 4051.043 kb on - strand, within sirB1 at 4051.133 kb on - strand, within sirB1 at 4051.306 kb on - strand, within sirB1 at 4051.306 kb on - strand, within sirB1 at 4051.306 kb on - strand, within sirB1 at 4051.306 kb on - strand, within sirB1 at 4051.439 kb on - strand, within sirB1 at 4051.454 kb on - strand, within sirB1 at 4051.502 kb on - strand, within sirB1 at 4051.502 kb on - strand, within sirB1 at 4051.540 kb on - strand, within sirB1 at 4051.726 kb on - strand at 4051.729 kb on - strand at 4051.745 kb on - strand at 4051.861 kb on - strand, within sirB2 at 4051.861 kb on - strand, within sirB2 at 4051.861 kb on - strand, within sirB2 at 4051.881 kb on + strand, within sirB2 at 4051.882 kb on - strand, within sirB2 at 4051.899 kb on - strand, within sirB2 at 4051.899 kb on - strand, within sirB2 at 4051.968 kb on + strand, within sirB2 at 4051.991 kb on - strand, within sirB2 at 4052.115 kb on + strand at 4052.116 kb on - strand at 4052.127 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECOR27 remove 4,050,948 - +0.6 4,050,967 - +0.1 4,051,043 - sirB1 NOLOHH_20595 0.12 +1.6 4,051,133 - sirB1 NOLOHH_20595 0.23 +1.6 4,051,306 - sirB1 NOLOHH_20595 0.44 -1.0 4,051,306 - sirB1 NOLOHH_20595 0.44 -0.2 4,051,306 - sirB1 NOLOHH_20595 0.44 -0.7 4,051,306 - sirB1 NOLOHH_20595 0.44 -1.0 4,051,439 - sirB1 NOLOHH_20595 0.61 -0.7 4,051,454 - sirB1 NOLOHH_20595 0.63 -0.4 4,051,502 - sirB1 NOLOHH_20595 0.69 +0.2 4,051,502 - sirB1 NOLOHH_20595 0.69 -0.6 4,051,540 - sirB1 NOLOHH_20595 0.73 +0.1 4,051,726 - -0.6 4,051,729 - +1.3 4,051,745 - +0.6 4,051,861 - sirB2 NOLOHH_20600 0.26 -2.0 4,051,861 - sirB2 NOLOHH_20600 0.26 +2.2 4,051,861 - sirB2 NOLOHH_20600 0.26 -0.4 4,051,881 + sirB2 NOLOHH_20600 0.31 +0.6 4,051,882 - sirB2 NOLOHH_20600 0.31 -0.2 4,051,899 - sirB2 NOLOHH_20600 0.36 -2.4 4,051,899 - sirB2 NOLOHH_20600 0.36 +0.6 4,051,968 + sirB2 NOLOHH_20600 0.53 +0.6 4,051,991 - sirB2 NOLOHH_20600 0.59 +0.6 4,052,115 + -1.4 4,052,116 - -0.4 4,052,127 + -1.0
Or see this region's nucleotide sequence