Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20295

Experiment: Control_ECOR27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciK and cobO overlap by 4 nucleotidescobO and rluB are separated by 39 nucleotidesrluB and yciO are separated by 100 nucleotidesyciO and yciV overlap by 4 nucleotides NOLOHH_20285: yciK - YciK family oxidoreductase, at 3,994,728 to 3,995,486 yciK NOLOHH_20290: cobO - cob(I)yrinic acid a,c-diamide adenosyltransferase, at 3,995,483 to 3,996,073 cobO NOLOHH_20295: rluB - 23S rRNA pseudouridine(2605) synthase RluB, at 3,996,113 to 3,996,985 rluB NOLOHH_20300: yciO - L-threonylcarbamoyladenylate synthase, at 3,997,086 to 3,997,706 yciO NOLOHH_20305: yciV - 5'-3' exoribonuclease, at 3,997,703 to 3,998,584 yciV Position (kb) 3996 3997Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3995.572 kb on + strand, within cobOat 3995.674 kb on + strand, within cobOat 3995.764 kb on + strand, within cobOat 3995.873 kb on + strand, within cobOat 3995.873 kb on + strand, within cobOat 3995.873 kb on + strand, within cobOat 3995.873 kb on + strand, within cobOat 3995.875 kb on + strand, within cobOat 3995.875 kb on + strand, within cobOat 3995.891 kb on + strand, within cobOat 3995.891 kb on + strand, within cobOat 3995.922 kb on + strand, within cobOat 3996.003 kb on + strand, within cobOat 3996.003 kb on + strand, within cobOat 3996.003 kb on + strand, within cobOat 3996.106 kb on - strandat 3996.124 kb on + strandat 3996.125 kb on - strandat 3996.125 kb on - strandat 3996.125 kb on - strandat 3996.230 kb on - strand, within rluBat 3996.275 kb on - strand, within rluBat 3996.275 kb on - strand, within rluBat 3996.669 kb on - strand, within rluBat 3996.746 kb on - strand, within rluBat 3996.746 kb on - strand, within rluBat 3996.769 kb on - strand, within rluBat 3996.985 kb on + strandat 3996.986 kb on - strandat 3996.986 kb on - strandat 3997.057 kb on - strandat 3997.067 kb on - strandat 3997.070 kb on - strandat 3997.071 kb on + strandat 3997.072 kb on - strandat 3997.087 kb on + strandat 3997.087 kb on + strandat 3997.088 kb on - strandat 3997.090 kb on - strandat 3997.111 kb on - strandat 3997.251 kb on - strand, within yciOat 3997.416 kb on - strand, within yciOat 3997.416 kb on - strand, within yciOat 3997.417 kb on + strand, within yciOat 3997.418 kb on - strand, within yciOat 3997.551 kb on - strand, within yciOat 3997.677 kb on - strandat 3997.706 kb on + strandat 3997.842 kb on - strand, within yciVat 3997.877 kb on - strand, within yciVat 3997.967 kb on - strand, within yciVat 3997.973 kb on - strand, within yciV

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECOR27
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3,995,572 + cobO NOLOHH_20290 0.15 +0.6
3,995,674 + cobO NOLOHH_20290 0.32 -0.2
3,995,764 + cobO NOLOHH_20290 0.48 -1.2
3,995,873 + cobO NOLOHH_20290 0.66 +0.8
3,995,873 + cobO NOLOHH_20290 0.66 +0.0
3,995,873 + cobO NOLOHH_20290 0.66 +0.0
3,995,873 + cobO NOLOHH_20290 0.66 -0.4
3,995,875 + cobO NOLOHH_20290 0.66 -1.0
3,995,875 + cobO NOLOHH_20290 0.66 -1.0
3,995,891 + cobO NOLOHH_20290 0.69 +0.6
3,995,891 + cobO NOLOHH_20290 0.69 -0.7
3,995,922 + cobO NOLOHH_20290 0.74 +0.0
3,996,003 + cobO NOLOHH_20290 0.88 +0.0
3,996,003 + cobO NOLOHH_20290 0.88 +0.6
3,996,003 + cobO NOLOHH_20290 0.88 +0.6
3,996,106 - +0.1
3,996,124 + -0.4
3,996,125 - +1.0
3,996,125 - +0.3
3,996,125 - -0.1
3,996,230 - rluB NOLOHH_20295 0.13 +0.6
3,996,275 - rluB NOLOHH_20295 0.19 -2.6
3,996,275 - rluB NOLOHH_20295 0.19 +1.6
3,996,669 - rluB NOLOHH_20295 0.64 -1.3
3,996,746 - rluB NOLOHH_20295 0.73 -2.9
3,996,746 - rluB NOLOHH_20295 0.73 +1.6
3,996,769 - rluB NOLOHH_20295 0.75 -1.7
3,996,985 + -1.7
3,996,986 - -1.7
3,996,986 - -0.7
3,997,057 - +1.3
3,997,067 - -1.2
3,997,070 - +0.6
3,997,071 + +2.2
3,997,072 - +0.9
3,997,087 + -1.7
3,997,087 + -0.4
3,997,088 - -2.2
3,997,090 - -0.6
3,997,111 - +1.2
3,997,251 - yciO NOLOHH_20300 0.27 -1.2
3,997,416 - yciO NOLOHH_20300 0.53 +0.6
3,997,416 - yciO NOLOHH_20300 0.53 +0.6
3,997,417 + yciO NOLOHH_20300 0.53 -1.4
3,997,418 - yciO NOLOHH_20300 0.53 +2.2
3,997,551 - yciO NOLOHH_20300 0.75 -1.0
3,997,677 - +0.9
3,997,706 + +1.2
3,997,842 - yciV NOLOHH_20305 0.16 +0.6
3,997,877 - yciV NOLOHH_20305 0.20 +0.2
3,997,967 - yciV NOLOHH_20305 0.30 -1.1
3,997,973 - yciV NOLOHH_20305 0.31 +0.0

Or see this region's nucleotide sequence