Strain Fitness in Escherichia coli ECOR27 around NOLOHH_18150

Experiment: Control_ECOR27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmanA and fumB are separated by 198 nucleotidesfumB and fumC are separated by 141 nucleotides NOLOHH_18145: manA - mannose-6-phosphate isomerase, at 3,563,291 to 3,564,466 manA NOLOHH_18150: fumB - class I fumarate hydratase, at 3,564,665 to 3,566,311 fumB NOLOHH_18155: fumC - class II fumarate hydratase, at 3,566,453 to 3,567,856 fumC Position (kb) 3564 3565 3566 3567Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3563.899 kb on + strand, within manAat 3563.900 kb on - strand, within manAat 3564.088 kb on + strand, within manAat 3564.089 kb on - strand, within manAat 3564.090 kb on + strand, within manAat 3564.095 kb on - strand, within manAat 3564.495 kb on - strandat 3564.555 kb on + strandat 3564.555 kb on + strandat 3564.728 kb on + strandat 3564.728 kb on + strandat 3564.729 kb on - strandat 3564.844 kb on + strand, within fumBat 3564.917 kb on + strand, within fumBat 3564.917 kb on + strand, within fumBat 3564.917 kb on + strand, within fumBat 3564.917 kb on + strand, within fumBat 3564.917 kb on + strand, within fumBat 3565.079 kb on + strand, within fumBat 3565.081 kb on + strand, within fumBat 3565.123 kb on + strand, within fumBat 3565.123 kb on + strand, within fumBat 3565.127 kb on + strand, within fumBat 3565.127 kb on + strand, within fumBat 3565.127 kb on + strand, within fumBat 3565.127 kb on + strand, within fumBat 3565.128 kb on - strand, within fumBat 3565.128 kb on - strand, within fumBat 3565.129 kb on + strand, within fumBat 3565.155 kb on + strand, within fumBat 3565.244 kb on + strand, within fumBat 3565.244 kb on + strand, within fumBat 3565.272 kb on + strand, within fumBat 3565.348 kb on + strand, within fumBat 3565.418 kb on + strand, within fumBat 3565.421 kb on + strand, within fumBat 3565.633 kb on + strand, within fumBat 3565.684 kb on + strand, within fumBat 3565.697 kb on + strand, within fumBat 3565.697 kb on + strand, within fumBat 3565.697 kb on + strand, within fumBat 3565.804 kb on + strand, within fumBat 3565.847 kb on - strand, within fumBat 3565.901 kb on + strand, within fumBat 3565.901 kb on + strand, within fumBat 3566.003 kb on + strand, within fumBat 3566.105 kb on + strand, within fumBat 3566.136 kb on + strand, within fumBat 3566.136 kb on + strand, within fumBat 3566.155 kb on + strandat 3566.277 kb on + strandat 3566.277 kb on + strandat 3566.458 kb on + strandat 3567.094 kb on + strand, within fumCat 3567.159 kb on + strand, within fumC

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECOR27
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3,563,899 + manA NOLOHH_18145 0.52 -1.0
3,563,900 - manA NOLOHH_18145 0.52 +0.6
3,564,088 + manA NOLOHH_18145 0.68 +2.2
3,564,089 - manA NOLOHH_18145 0.68 -0.4
3,564,090 + manA NOLOHH_18145 0.68 -0.4
3,564,095 - manA NOLOHH_18145 0.68 -0.4
3,564,495 - +0.6
3,564,555 + +1.0
3,564,555 + +1.9
3,564,728 + +2.2
3,564,728 + +0.6
3,564,729 - -1.4
3,564,844 + fumB NOLOHH_18150 0.11 +2.9
3,564,917 + fumB NOLOHH_18150 0.15 +2.9
3,564,917 + fumB NOLOHH_18150 0.15 +0.6
3,564,917 + fumB NOLOHH_18150 0.15 -1.9
3,564,917 + fumB NOLOHH_18150 0.15 -0.2
3,564,917 + fumB NOLOHH_18150 0.15 +0.6
3,565,079 + fumB NOLOHH_18150 0.25 +1.6
3,565,081 + fumB NOLOHH_18150 0.25 +0.6
3,565,123 + fumB NOLOHH_18150 0.28 -0.4
3,565,123 + fumB NOLOHH_18150 0.28 -0.4
3,565,127 + fumB NOLOHH_18150 0.28 -0.4
3,565,127 + fumB NOLOHH_18150 0.28 -2.0
3,565,127 + fumB NOLOHH_18150 0.28 -0.8
3,565,127 + fumB NOLOHH_18150 0.28 +0.6
3,565,128 - fumB NOLOHH_18150 0.28 +0.6
3,565,128 - fumB NOLOHH_18150 0.28 +1.6
3,565,129 + fumB NOLOHH_18150 0.28 -0.7
3,565,155 + fumB NOLOHH_18150 0.30 -0.0
3,565,244 + fumB NOLOHH_18150 0.35 -0.4
3,565,244 + fumB NOLOHH_18150 0.35 -1.4
3,565,272 + fumB NOLOHH_18150 0.37 +0.6
3,565,348 + fumB NOLOHH_18150 0.41 +1.6
3,565,418 + fumB NOLOHH_18150 0.46 -1.8
3,565,421 + fumB NOLOHH_18150 0.46 +2.2
3,565,633 + fumB NOLOHH_18150 0.59 -0.0
3,565,684 + fumB NOLOHH_18150 0.62 +1.6
3,565,697 + fumB NOLOHH_18150 0.63 -1.4
3,565,697 + fumB NOLOHH_18150 0.63 +0.6
3,565,697 + fumB NOLOHH_18150 0.63 -1.0
3,565,804 + fumB NOLOHH_18150 0.69 -0.4
3,565,847 - fumB NOLOHH_18150 0.72 +1.6
3,565,901 + fumB NOLOHH_18150 0.75 +1.3
3,565,901 + fumB NOLOHH_18150 0.75 +0.2
3,566,003 + fumB NOLOHH_18150 0.81 +1.6
3,566,105 + fumB NOLOHH_18150 0.87 +1.3
3,566,136 + fumB NOLOHH_18150 0.89 -0.4
3,566,136 + fumB NOLOHH_18150 0.89 +3.4
3,566,155 + +0.2
3,566,277 + -0.9
3,566,277 + -0.0
3,566,458 + -1.0
3,567,094 + fumC NOLOHH_18155 0.46 -0.4
3,567,159 + fumC NOLOHH_18155 0.50 +0.9

Or see this region's nucleotide sequence