Strain Fitness in Escherichia coli ECOR27 around NOLOHH_13520

Experiment: Control_ECOR27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyfJ and NOLOHH_13515 are separated by 228 nucleotidesNOLOHH_13515 and hyfH overlap by 4 nucleotideshyfH and hyfG are separated by 9 nucleotides NOLOHH_13510: hyfJ - hydrogenase 4 assembly chaperone HyfJ, at 2,626,081 to 2,626,494 hyfJ NOLOHH_13515: NOLOHH_13515 - Formate hydrogenlyase subunit 7, at 2,626,723 to 2,627,244 _13515 NOLOHH_13520: hyfH - hydrogenase 4 subunit H, at 2,627,241 to 2,627,786 hyfH NOLOHH_13525: hyfG - hydrogenase 4 catalytic subunit HyfG, at 2,627,796 to 2,629,511 hyfG Position (kb) 2627 2628Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2626.275 kb on + strand, within hyfJat 2626.276 kb on - strand, within hyfJat 2626.394 kb on + strand, within hyfJat 2626.397 kb on + strand, within hyfJat 2626.398 kb on - strand, within hyfJat 2626.398 kb on - strand, within hyfJat 2626.398 kb on - strand, within hyfJat 2626.398 kb on - strand, within hyfJat 2626.398 kb on - strand, within hyfJat 2626.398 kb on - strand, within hyfJat 2626.398 kb on - strand, within hyfJat 2626.398 kb on - strand, within hyfJat 2626.496 kb on + strandat 2626.497 kb on - strandat 2626.531 kb on + strandat 2626.531 kb on + strandat 2626.532 kb on - strandat 2626.534 kb on + strandat 2626.601 kb on + strandat 2626.720 kb on + strandat 2626.938 kb on + strand, within NOLOHH_13515at 2626.939 kb on - strand, within NOLOHH_13515at 2626.939 kb on - strand, within NOLOHH_13515at 2626.959 kb on - strand, within NOLOHH_13515at 2626.959 kb on - strand, within NOLOHH_13515at 2626.977 kb on + strand, within NOLOHH_13515at 2626.978 kb on - strand, within NOLOHH_13515at 2627.004 kb on + strand, within NOLOHH_13515at 2627.005 kb on - strand, within NOLOHH_13515at 2627.005 kb on - strand, within NOLOHH_13515at 2627.017 kb on - strand, within NOLOHH_13515at 2627.040 kb on + strand, within NOLOHH_13515at 2627.046 kb on + strand, within NOLOHH_13515at 2627.047 kb on - strand, within NOLOHH_13515at 2627.072 kb on - strand, within NOLOHH_13515at 2627.141 kb on + strand, within NOLOHH_13515at 2627.164 kb on + strand, within NOLOHH_13515at 2627.229 kb on + strandat 2627.268 kb on + strandat 2627.268 kb on + strandat 2627.445 kb on + strand, within hyfHat 2627.446 kb on - strand, within hyfHat 2627.446 kb on - strand, within hyfHat 2627.446 kb on - strand, within hyfHat 2627.467 kb on + strand, within hyfHat 2627.467 kb on + strand, within hyfHat 2627.467 kb on + strand, within hyfHat 2627.510 kb on + strand, within hyfHat 2627.511 kb on - strand, within hyfHat 2627.561 kb on + strand, within hyfHat 2627.561 kb on + strand, within hyfHat 2627.561 kb on + strand, within hyfHat 2627.562 kb on - strand, within hyfHat 2627.562 kb on - strand, within hyfHat 2627.575 kb on + strand, within hyfHat 2627.575 kb on + strand, within hyfHat 2627.576 kb on - strand, within hyfHat 2627.666 kb on + strand, within hyfHat 2627.666 kb on + strand, within hyfHat 2627.667 kb on - strand, within hyfHat 2627.667 kb on - strand, within hyfHat 2627.735 kb on - strandat 2627.766 kb on - strandat 2627.766 kb on - strandat 2627.775 kb on + strandat 2627.797 kb on + strandat 2627.798 kb on - strandat 2627.884 kb on - strandat 2627.911 kb on + strandat 2627.911 kb on + strandat 2627.975 kb on - strand, within hyfGat 2627.999 kb on - strand, within hyfGat 2628.038 kb on + strand, within hyfGat 2628.113 kb on + strand, within hyfGat 2628.113 kb on + strand, within hyfGat 2628.114 kb on - strand, within hyfGat 2628.115 kb on + strand, within hyfGat 2628.116 kb on - strand, within hyfGat 2628.119 kb on + strand, within hyfGat 2628.119 kb on + strand, within hyfGat 2628.119 kb on + strand, within hyfGat 2628.120 kb on - strand, within hyfGat 2628.120 kb on - strand, within hyfGat 2628.245 kb on + strand, within hyfGat 2628.263 kb on + strand, within hyfGat 2628.344 kb on + strand, within hyfGat 2628.344 kb on + strand, within hyfGat 2628.345 kb on - strand, within hyfGat 2628.345 kb on - strand, within hyfGat 2628.533 kb on - strand, within hyfGat 2628.539 kb on + strand, within hyfGat 2628.540 kb on - strand, within hyfGat 2628.647 kb on + strand, within hyfGat 2628.696 kb on - strand, within hyfGat 2628.696 kb on - strand, within hyfGat 2628.696 kb on - strand, within hyfGat 2628.698 kb on + strand, within hyfGat 2628.699 kb on - strand, within hyfGat 2628.699 kb on - strand, within hyfGat 2628.699 kb on - strand, within hyfGat 2628.699 kb on - strand, within hyfGat 2628.699 kb on - strand, within hyfGat 2628.752 kb on - strand, within hyfG

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECOR27
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2,626,275 + hyfJ NOLOHH_13510 0.47 -0.2
2,626,276 - hyfJ NOLOHH_13510 0.47 +0.3
2,626,394 + hyfJ NOLOHH_13510 0.76 +0.4
2,626,397 + hyfJ NOLOHH_13510 0.76 +0.6
2,626,398 - hyfJ NOLOHH_13510 0.77 +0.6
2,626,398 - hyfJ NOLOHH_13510 0.77 +1.3
2,626,398 - hyfJ NOLOHH_13510 0.77 +0.7
2,626,398 - hyfJ NOLOHH_13510 0.77 +0.3
2,626,398 - hyfJ NOLOHH_13510 0.77 -0.6
2,626,398 - hyfJ NOLOHH_13510 0.77 +1.1
2,626,398 - hyfJ NOLOHH_13510 0.77 +1.6
2,626,398 - hyfJ NOLOHH_13510 0.77 +0.4
2,626,496 + -0.4
2,626,497 - +0.5
2,626,531 + -1.4
2,626,531 + -1.7
2,626,532 - -1.7
2,626,534 + -1.2
2,626,601 + -0.4
2,626,720 + +2.6
2,626,938 + NOLOHH_13515 0.41 -0.3
2,626,939 - NOLOHH_13515 0.41 -1.2
2,626,939 - NOLOHH_13515 0.41 +1.2
2,626,959 - NOLOHH_13515 0.45 +1.2
2,626,959 - NOLOHH_13515 0.45 -0.1
2,626,977 + NOLOHH_13515 0.49 -0.0
2,626,978 - NOLOHH_13515 0.49 -1.2
2,627,004 + NOLOHH_13515 0.54 -0.2
2,627,005 - NOLOHH_13515 0.54 -1.2
2,627,005 - NOLOHH_13515 0.54 -0.7
2,627,017 - NOLOHH_13515 0.56 -0.6
2,627,040 + NOLOHH_13515 0.61 -0.4
2,627,046 + NOLOHH_13515 0.62 -0.5
2,627,047 - NOLOHH_13515 0.62 +1.6
2,627,072 - NOLOHH_13515 0.67 +1.2
2,627,141 + NOLOHH_13515 0.80 -0.1
2,627,164 + NOLOHH_13515 0.84 +1.0
2,627,229 + +0.1
2,627,268 + +0.6
2,627,268 + -0.1
2,627,445 + hyfH NOLOHH_13520 0.37 -0.9
2,627,446 - hyfH NOLOHH_13520 0.38 -0.7
2,627,446 - hyfH NOLOHH_13520 0.38 +1.1
2,627,446 - hyfH NOLOHH_13520 0.38 +0.1
2,627,467 + hyfH NOLOHH_13520 0.41 -1.3
2,627,467 + hyfH NOLOHH_13520 0.41 -0.4
2,627,467 + hyfH NOLOHH_13520 0.41 -0.6
2,627,510 + hyfH NOLOHH_13520 0.49 -0.7
2,627,511 - hyfH NOLOHH_13520 0.49 -1.0
2,627,561 + hyfH NOLOHH_13520 0.59 -1.4
2,627,561 + hyfH NOLOHH_13520 0.59 +1.6
2,627,561 + hyfH NOLOHH_13520 0.59 -0.3
2,627,562 - hyfH NOLOHH_13520 0.59 +1.6
2,627,562 - hyfH NOLOHH_13520 0.59 +0.6
2,627,575 + hyfH NOLOHH_13520 0.61 -1.1
2,627,575 + hyfH NOLOHH_13520 0.61 -0.2
2,627,576 - hyfH NOLOHH_13520 0.61 -2.4
2,627,666 + hyfH NOLOHH_13520 0.78 -0.4
2,627,666 + hyfH NOLOHH_13520 0.78 -0.4
2,627,667 - hyfH NOLOHH_13520 0.78 +2.6
2,627,667 - hyfH NOLOHH_13520 0.78 +2.6
2,627,735 - -1.0
2,627,766 - +0.6
2,627,766 - +0.6
2,627,775 + -0.4
2,627,797 + -0.4
2,627,798 - -0.0
2,627,884 - +2.9
2,627,911 + +0.3
2,627,911 + +0.6
2,627,975 - hyfG NOLOHH_13525 0.10 -1.7
2,627,999 - hyfG NOLOHH_13525 0.12 -0.6
2,628,038 + hyfG NOLOHH_13525 0.14 -1.4
2,628,113 + hyfG NOLOHH_13525 0.18 -0.5
2,628,113 + hyfG NOLOHH_13525 0.18 +0.2
2,628,114 - hyfG NOLOHH_13525 0.19 -0.2
2,628,115 + hyfG NOLOHH_13525 0.19 +0.6
2,628,116 - hyfG NOLOHH_13525 0.19 -0.6
2,628,119 + hyfG NOLOHH_13525 0.19 -0.4
2,628,119 + hyfG NOLOHH_13525 0.19 +0.7
2,628,119 + hyfG NOLOHH_13525 0.19 -0.4
2,628,120 - hyfG NOLOHH_13525 0.19 -0.0
2,628,120 - hyfG NOLOHH_13525 0.19 -0.0
2,628,245 + hyfG NOLOHH_13525 0.26 +0.8
2,628,263 + hyfG NOLOHH_13525 0.27 -0.4
2,628,344 + hyfG NOLOHH_13525 0.32 -0.4
2,628,344 + hyfG NOLOHH_13525 0.32 -1.0
2,628,345 - hyfG NOLOHH_13525 0.32 -0.2
2,628,345 - hyfG NOLOHH_13525 0.32 -0.0
2,628,533 - hyfG NOLOHH_13525 0.43 -0.0
2,628,539 + hyfG NOLOHH_13525 0.43 +0.6
2,628,540 - hyfG NOLOHH_13525 0.43 -0.2
2,628,647 + hyfG NOLOHH_13525 0.50 +0.1
2,628,696 - hyfG NOLOHH_13525 0.52 -1.0
2,628,696 - hyfG NOLOHH_13525 0.52 -0.2
2,628,696 - hyfG NOLOHH_13525 0.52 +1.6
2,628,698 + hyfG NOLOHH_13525 0.53 +0.6
2,628,699 - hyfG NOLOHH_13525 0.53 -0.0
2,628,699 - hyfG NOLOHH_13525 0.53 +0.6
2,628,699 - hyfG NOLOHH_13525 0.53 -0.4
2,628,699 - hyfG NOLOHH_13525 0.53 +0.6
2,628,699 - hyfG NOLOHH_13525 0.53 +0.6
2,628,752 - hyfG NOLOHH_13525 0.56 -0.0

Or see this region's nucleotide sequence