Experiment: Control_ECOR27
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt arfB and yaeQ overlap by 4 nucleotides yaeQ and yaeP are separated by 165 nucleotides yaeP and rof overlap by 14 nucleotides rof and tilS are separated by 48 nucleotides
NOLOHH_02440: arfB - aminoacyl-tRNA hydrolase, at 395,134 to 395,556
arfB
NOLOHH_02445: yaeQ - Uncharacterized protein YaeQ, at 395,553 to 396,098
yaeQ
NOLOHH_02450: yaeP - YaeP family protein, at 396,264 to 396,464
yaeP
NOLOHH_02455: rof - Rho-binding antiterminator, at 396,451 to 396,711
rof
NOLOHH_02460: tilS - tRNA lysidine(34) synthetase TilS, at 396,760 to 398,058
tilS
Position (kb) 396
397 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 395.264 kb on - strand, within arfB at 395.402 kb on + strand, within arfB at 395.403 kb on - strand, within arfB at 395.403 kb on - strand, within arfB at 395.403 kb on - strand, within arfB at 395.463 kb on + strand, within arfB at 395.467 kb on - strand, within arfB at 395.582 kb on + strand at 395.583 kb on - strand at 395.674 kb on - strand, within yaeQ at 395.702 kb on - strand, within yaeQ at 395.775 kb on - strand, within yaeQ at 395.775 kb on - strand, within yaeQ at 395.777 kb on + strand, within yaeQ at 395.777 kb on + strand, within yaeQ at 395.777 kb on + strand, within yaeQ at 395.778 kb on - strand, within yaeQ at 395.778 kb on - strand, within yaeQ at 395.780 kb on - strand, within yaeQ at 395.780 kb on - strand, within yaeQ at 395.783 kb on + strand, within yaeQ at 395.784 kb on - strand, within yaeQ at 395.784 kb on - strand, within yaeQ at 395.950 kb on + strand, within yaeQ at 395.951 kb on - strand, within yaeQ at 395.981 kb on + strand, within yaeQ at 395.982 kb on - strand, within yaeQ at 396.074 kb on + strand at 396.075 kb on - strand at 396.139 kb on + strand at 396.139 kb on + strand at 396.180 kb on + strand at 396.180 kb on + strand at 396.181 kb on - strand at 396.182 kb on + strand at 396.183 kb on - strand at 396.205 kb on + strand at 396.214 kb on - strand at 396.238 kb on - strand at 396.283 kb on + strand at 396.286 kb on + strand, within yaeP at 396.322 kb on + strand, within yaeP at 396.322 kb on + strand, within yaeP at 396.323 kb on - strand, within yaeP at 396.327 kb on + strand, within yaeP at 396.420 kb on + strand, within yaeP at 396.420 kb on + strand, within yaeP at 396.421 kb on - strand, within yaeP at 396.436 kb on + strand, within yaeP at 396.450 kb on + strand at 396.450 kb on + strand at 396.450 kb on + strand at 396.451 kb on - strand at 396.465 kb on + strand at 396.469 kb on + strand at 396.469 kb on + strand at 396.469 kb on + strand at 396.469 kb on + strand at 396.469 kb on + strand at 396.493 kb on + strand, within rof at 396.493 kb on + strand, within rof at 396.527 kb on + strand, within rof at 396.608 kb on - strand, within rof at 396.645 kb on - strand, within rof at 396.645 kb on - strand, within rof at 396.661 kb on - strand, within rof at 396.761 kb on + strand at 396.761 kb on + strand at 396.766 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECOR27 remove 395,264 - arfB NOLOHH_02440 0.31 -1.4 395,402 + arfB NOLOHH_02440 0.63 +0.2 395,403 - arfB NOLOHH_02440 0.64 -0.6 395,403 - arfB NOLOHH_02440 0.64 -0.2 395,403 - arfB NOLOHH_02440 0.64 -0.4 395,463 + arfB NOLOHH_02440 0.78 -1.0 395,467 - arfB NOLOHH_02440 0.79 +0.8 395,582 + -0.5 395,583 - -1.0 395,674 - yaeQ NOLOHH_02445 0.22 -1.0 395,702 - yaeQ NOLOHH_02445 0.27 +0.6 395,775 - yaeQ NOLOHH_02445 0.41 -3.6 395,775 - yaeQ NOLOHH_02445 0.41 -1.5 395,777 + yaeQ NOLOHH_02445 0.41 +0.2 395,777 + yaeQ NOLOHH_02445 0.41 +0.6 395,777 + yaeQ NOLOHH_02445 0.41 -0.6 395,778 - yaeQ NOLOHH_02445 0.41 +0.4 395,778 - yaeQ NOLOHH_02445 0.41 -0.4 395,780 - yaeQ NOLOHH_02445 0.42 +0.1 395,780 - yaeQ NOLOHH_02445 0.42 -0.4 395,783 + yaeQ NOLOHH_02445 0.42 +0.0 395,784 - yaeQ NOLOHH_02445 0.42 -1.4 395,784 - yaeQ NOLOHH_02445 0.42 +0.5 395,950 + yaeQ NOLOHH_02445 0.73 +0.9 395,951 - yaeQ NOLOHH_02445 0.73 +0.8 395,981 + yaeQ NOLOHH_02445 0.78 +0.5 395,982 - yaeQ NOLOHH_02445 0.79 -0.2 396,074 + -0.4 396,075 - -0.7 396,139 + +2.6 396,139 + +0.9 396,180 + +1.4 396,180 + -1.3 396,181 - +0.8 396,182 + -0.0 396,183 - -0.8 396,205 + +0.0 396,214 - +1.6 396,238 - +0.6 396,283 + +1.5 396,286 + yaeP NOLOHH_02450 0.11 +0.6 396,322 + yaeP NOLOHH_02450 0.29 -1.2 396,322 + yaeP NOLOHH_02450 0.29 -0.4 396,323 - yaeP NOLOHH_02450 0.29 -0.6 396,327 + yaeP NOLOHH_02450 0.31 -0.8 396,420 + yaeP NOLOHH_02450 0.78 -1.0 396,420 + yaeP NOLOHH_02450 0.78 -0.5 396,421 - yaeP NOLOHH_02450 0.78 -0.4 396,436 + yaeP NOLOHH_02450 0.86 +0.0 396,450 + +0.6 396,450 + -0.6 396,450 + -0.6 396,451 - -1.4 396,465 + +0.6 396,469 + +0.2 396,469 + +1.6 396,469 + -0.3 396,469 + -2.0 396,469 + +0.6 396,493 + rof NOLOHH_02455 0.16 -1.4 396,493 + rof NOLOHH_02455 0.16 +1.9 396,527 + rof NOLOHH_02455 0.29 +0.6 396,608 - rof NOLOHH_02455 0.60 -1.0 396,645 - rof NOLOHH_02455 0.74 -0.4 396,645 - rof NOLOHH_02455 0.74 +0.6 396,661 - rof NOLOHH_02455 0.80 -0.1 396,761 + +0.6 396,761 + -1.4 396,766 - +0.2
Or see this region's nucleotide sequence