Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt queF and OKGIIK_13400 are separated by 188 nucleotides OKGIIK_13400 and OKGIIK_13405 overlap by 30 nucleotides OKGIIK_13405 and OKGIIK_13410 are separated by 47 nucleotides OKGIIK_13410 and OKGIIK_13415 are separated by 228 nucleotides
OKGIIK_13395: queF - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 2,893,751 to 2,894,575
queF
OKGIIK_13400: OKGIIK_13400 - hypothetical protein, at 2,894,764 to 2,895,375
_13400
OKGIIK_13405: OKGIIK_13405 - IS3 family transposase, at 2,895,346 to 2,896,164
_13405
OKGIIK_13410: OKGIIK_13410 - Mobile element protein, at 2,896,212 to 2,896,499
_13410
OKGIIK_13415: OKGIIK_13415 - SseB family protein, at 2,896,728 to 2,897,267
_13415
Position (kb)
2895
2896
2897 Strain fitness (log2 ratio)
-2
-1
0
1 at 2894.378 kb on + strand, within queF at 2894.480 kb on + strand, within queF at 2894.481 kb on - strand, within queF at 2894.481 kb on - strand, within queF at 2894.565 kb on + strand at 2894.565 kb on + strand at 2894.574 kb on - strand at 2894.785 kb on - strand at 2894.785 kb on - strand at 2895.055 kb on + strand, within OKGIIK_13400 at 2895.056 kb on - strand, within OKGIIK_13400 at 2895.056 kb on - strand, within OKGIIK_13400 at 2895.062 kb on + strand, within OKGIIK_13400 at 2895.062 kb on + strand, within OKGIIK_13400 at 2895.063 kb on - strand, within OKGIIK_13400 at 2895.106 kb on + strand, within OKGIIK_13400 at 2895.106 kb on + strand, within OKGIIK_13400 at 2895.106 kb on + strand, within OKGIIK_13400 at 2895.106 kb on + strand, within OKGIIK_13400 at 2895.106 kb on + strand, within OKGIIK_13400 at 2895.106 kb on + strand, within OKGIIK_13400 at 2895.107 kb on - strand, within OKGIIK_13400 at 2895.107 kb on - strand, within OKGIIK_13400 at 2895.107 kb on - strand, within OKGIIK_13400 at 2895.107 kb on - strand, within OKGIIK_13400 at 2895.107 kb on - strand, within OKGIIK_13400 at 2895.166 kb on + strand, within OKGIIK_13400 at 2895.166 kb on + strand, within OKGIIK_13400 at 2895.167 kb on - strand, within OKGIIK_13400 at 2895.167 kb on - strand, within OKGIIK_13400 at 2895.280 kb on + strand, within OKGIIK_13400 at 2895.280 kb on + strand, within OKGIIK_13400 at 2895.280 kb on + strand, within OKGIIK_13400 at 2895.281 kb on - strand, within OKGIIK_13400 at 2895.281 kb on - strand, within OKGIIK_13400 at 2895.374 kb on - strand at 2895.374 kb on - strand at 2895.374 kb on - strand at 2896.531 kb on + strand at 2896.531 kb on + strand at 2896.531 kb on + strand at 2896.531 kb on + strand at 2896.602 kb on + strand at 2896.647 kb on + strand at 2896.647 kb on + strand at 2896.647 kb on + strand at 2896.648 kb on - strand at 2896.657 kb on + strand at 2896.657 kb on + strand at 2896.657 kb on + strand at 2896.658 kb on - strand at 2896.658 kb on - strand at 2896.658 kb on - strand at 2896.658 kb on - strand at 2896.658 kb on - strand at 2896.706 kb on - strand at 2896.706 kb on - strand at 2896.707 kb on - strand at 2896.848 kb on - strand, within OKGIIK_13415 at 2896.848 kb on - strand, within OKGIIK_13415 at 2896.939 kb on + strand, within OKGIIK_13415 at 2896.939 kb on + strand, within OKGIIK_13415 at 2896.940 kb on - strand, within OKGIIK_13415 at 2896.994 kb on - strand, within OKGIIK_13415 at 2897.035 kb on + strand, within OKGIIK_13415 at 2897.035 kb on + strand, within OKGIIK_13415 at 2897.035 kb on + strand, within OKGIIK_13415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 2,894,378 + queF OKGIIK_13395 0.76 -1.3 2,894,480 + queF OKGIIK_13395 0.88 -0.4 2,894,481 - queF OKGIIK_13395 0.88 -0.2 2,894,481 - queF OKGIIK_13395 0.88 +0.4 2,894,565 + +1.5 2,894,565 + -0.7 2,894,574 - +0.3 2,894,785 - +0.7 2,894,785 - +1.7 2,895,055 + OKGIIK_13400 0.48 -0.1 2,895,056 - OKGIIK_13400 0.48 +0.4 2,895,056 - OKGIIK_13400 0.48 +0.3 2,895,062 + OKGIIK_13400 0.49 +0.2 2,895,062 + OKGIIK_13400 0.49 -0.5 2,895,063 - OKGIIK_13400 0.49 +0.7 2,895,106 + OKGIIK_13400 0.56 +0.0 2,895,106 + OKGIIK_13400 0.56 -0.8 2,895,106 + OKGIIK_13400 0.56 -0.5 2,895,106 + OKGIIK_13400 0.56 -1.4 2,895,106 + OKGIIK_13400 0.56 -0.1 2,895,106 + OKGIIK_13400 0.56 -1.1 2,895,107 - OKGIIK_13400 0.56 +0.4 2,895,107 - OKGIIK_13400 0.56 -0.4 2,895,107 - OKGIIK_13400 0.56 +0.9 2,895,107 - OKGIIK_13400 0.56 -0.4 2,895,107 - OKGIIK_13400 0.56 +1.0 2,895,166 + OKGIIK_13400 0.66 +0.3 2,895,166 + OKGIIK_13400 0.66 +0.2 2,895,167 - OKGIIK_13400 0.66 -1.7 2,895,167 - OKGIIK_13400 0.66 -0.1 2,895,280 + OKGIIK_13400 0.84 +0.4 2,895,280 + OKGIIK_13400 0.84 -0.7 2,895,280 + OKGIIK_13400 0.84 +0.0 2,895,281 - OKGIIK_13400 0.84 -0.5 2,895,281 - OKGIIK_13400 0.84 -0.6 2,895,374 - -2.0 2,895,374 - +0.4 2,895,374 - -2.7 2,896,531 + -0.5 2,896,531 + +0.2 2,896,531 + -0.3 2,896,531 + -0.5 2,896,602 + -0.1 2,896,647 + -0.4 2,896,647 + -1.3 2,896,647 + -0.8 2,896,648 - +1.7 2,896,657 + -1.8 2,896,657 + -1.7 2,896,657 + +0.3 2,896,658 - -0.4 2,896,658 - -0.4 2,896,658 - -0.8 2,896,658 - -0.3 2,896,658 - -1.4 2,896,706 - -2.1 2,896,706 - +0.3 2,896,707 - +0.2 2,896,848 - OKGIIK_13415 0.22 +0.3 2,896,848 - OKGIIK_13415 0.22 -2.1 2,896,939 + OKGIIK_13415 0.39 +0.3 2,896,939 + OKGIIK_13415 0.39 -0.6 2,896,940 - OKGIIK_13415 0.39 -0.7 2,896,994 - OKGIIK_13415 0.49 +1.3 2,897,035 + OKGIIK_13415 0.57 +0.3 2,897,035 + OKGIIK_13415 0.57 +1.1 2,897,035 + OKGIIK_13415 0.57 -0.1
Or see this region's nucleotide sequence