Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kup and ruvB are separated by 118 nucleotides ruvB and ybgC overlap by 8 nucleotides ybgC and tolQ are separated by 39 nucleotides
OKGIIK_01845: kup - potassium uptake protein, at 427,592 to 429,493
kup
OKGIIK_01850: ruvB - Holliday junction branch migration DNA helicase RuvB, at 429,612 to 430,661
ruvB
OKGIIK_01855: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 430,654 to 431,073
ybgC
OKGIIK_01860: tolQ - protein TolQ, at 431,113 to 431,799
tolQ
Position (kb) 429
430
431 Strain fitness (log2 ratio) -2
-1
0
1
2 at 429.319 kb on + strand at 429.319 kb on + strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.543 kb on + strand at 429.543 kb on + strand at 429.544 kb on - strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 430.159 kb on - strand, within ruvB at 430.694 kb on + strand at 430.694 kb on + strand at 430.694 kb on + strand at 430.694 kb on + strand at 430.694 kb on + strand at 430.694 kb on + strand at 430.694 kb on + strand at 430.695 kb on - strand at 430.695 kb on - strand at 430.695 kb on - strand at 430.702 kb on + strand, within ybgC at 430.702 kb on + strand, within ybgC at 430.702 kb on + strand, within ybgC at 430.702 kb on + strand, within ybgC at 430.702 kb on + strand, within ybgC at 430.702 kb on + strand, within ybgC at 430.703 kb on - strand, within ybgC at 430.735 kb on + strand, within ybgC at 430.735 kb on + strand, within ybgC at 430.735 kb on + strand, within ybgC at 430.735 kb on + strand, within ybgC at 430.735 kb on + strand, within ybgC at 430.735 kb on + strand, within ybgC at 430.736 kb on - strand, within ybgC at 430.736 kb on - strand, within ybgC at 430.736 kb on - strand, within ybgC at 430.736 kb on - strand, within ybgC at 430.736 kb on - strand, within ybgC at 430.748 kb on - strand, within ybgC at 430.935 kb on + strand, within ybgC at 430.935 kb on + strand, within ybgC at 430.935 kb on + strand, within ybgC at 430.936 kb on - strand, within ybgC at 430.936 kb on - strand, within ybgC
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 429,319 + -0.6 429,319 + -1.6 429,505 - -2.4 429,505 - +2.1 429,505 - -2.6 429,505 - -0.4 429,505 - -0.6 429,505 - +0.5 429,541 + -1.8 429,541 + -0.6 429,541 + -1.6 429,541 + -1.2 429,541 + +0.5 429,541 + -0.6 429,541 + -0.2 429,541 + -0.8 429,541 + -0.2 429,541 + +0.4 429,541 + +0.7 429,541 + -0.9 429,541 + +1.2 429,541 + +1.8 429,541 + +1.4 429,542 - +0.2 429,542 - +2.2 429,542 - -0.0 429,542 - +0.5 429,542 - -0.6 429,542 - +1.6 429,542 - -0.9 429,542 - -0.1 429,542 - -1.5 429,542 - +0.4 429,542 - +1.4 429,542 - -0.9 429,542 - +0.6 429,542 - +1.7 429,542 - +0.5 429,543 + -0.2 429,543 + +0.1 429,544 - -0.5 429,578 + -0.9 429,578 + -0.5 429,578 + +0.5 429,578 + +0.1 429,584 + +1.0 429,584 + +0.9 429,584 + +0.3 429,584 + -0.9 429,584 + -1.9 429,584 + +0.2 429,584 + -0.1 429,584 + +0.2 429,584 + -0.4 429,584 + -0.1 429,584 + -0.9 429,584 + +1.1 429,584 + -1.3 429,584 + +0.6 429,584 + +0.2 429,584 + -0.6 429,584 + -0.6 429,584 + -0.5 429,584 + +1.0 429,584 + -1.6 429,584 + -0.8 429,584 + -0.9 430,159 - ruvB OKGIIK_01850 0.52 +1.0 430,694 + +0.9 430,694 + -0.3 430,694 + -2.0 430,694 + -1.2 430,694 + -1.2 430,694 + +0.4 430,694 + -0.1 430,695 - -1.8 430,695 - +1.8 430,695 - -0.6 430,702 + ybgC OKGIIK_01855 0.11 -0.1 430,702 + ybgC OKGIIK_01855 0.11 -1.0 430,702 + ybgC OKGIIK_01855 0.11 +0.4 430,702 + ybgC OKGIIK_01855 0.11 -1.2 430,702 + ybgC OKGIIK_01855 0.11 +0.7 430,702 + ybgC OKGIIK_01855 0.11 -1.8 430,703 - ybgC OKGIIK_01855 0.12 -0.1 430,735 + ybgC OKGIIK_01855 0.19 -1.8 430,735 + ybgC OKGIIK_01855 0.19 -0.6 430,735 + ybgC OKGIIK_01855 0.19 -2.1 430,735 + ybgC OKGIIK_01855 0.19 -0.5 430,735 + ybgC OKGIIK_01855 0.19 -0.9 430,735 + ybgC OKGIIK_01855 0.19 -0.0 430,736 - ybgC OKGIIK_01855 0.20 +1.4 430,736 - ybgC OKGIIK_01855 0.20 -0.6 430,736 - ybgC OKGIIK_01855 0.20 -0.9 430,736 - ybgC OKGIIK_01855 0.20 +1.4 430,736 - ybgC OKGIIK_01855 0.20 +0.1 430,748 - ybgC OKGIIK_01855 0.22 -2.1 430,935 + ybgC OKGIIK_01855 0.67 -1.9 430,935 + ybgC OKGIIK_01855 0.67 -0.6 430,935 + ybgC OKGIIK_01855 0.67 -0.3 430,936 - ybgC OKGIIK_01855 0.67 -0.9 430,936 - ybgC OKGIIK_01855 0.67 -0.2
Or see this region's nucleotide sequence