Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fadE and OKGIIK_01405 are separated by 56 nucleotides OKGIIK_01405 and OKGIIK_01410 are separated by 19 nucleotides OKGIIK_01410 and OKGIIK_01415 are separated by 32 nucleotides OKGIIK_01415 and OKGIIK_01420 are separated by 43 nucleotides
OKGIIK_01400: fadE - acyl-CoA dehydrogenase, at 310,464 to 312,914
fadE
OKGIIK_01405: OKGIIK_01405 - DUF4442 domain-containing protein, at 312,971 to 313,444
_01405
OKGIIK_01410: OKGIIK_01410 - Histidine kinase, at 313,464 to 314,465
_01410
OKGIIK_01415: OKGIIK_01415 - hypothetical protein, at 314,498 to 314,623
_01415
OKGIIK_01420: OKGIIK_01420 - Histidine kinase, at 314,667 to 318,299
_01420
Position (kb) 313
314
315 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 312.624 kb on + strand, within fadE at 312.624 kb on + strand, within fadE at 312.625 kb on - strand, within fadE at 312.625 kb on - strand, within fadE at 312.625 kb on - strand, within fadE at 312.625 kb on - strand, within fadE at 312.625 kb on - strand, within fadE at 312.625 kb on - strand, within fadE at 312.753 kb on + strand at 312.753 kb on + strand at 312.753 kb on + strand at 312.753 kb on + strand at 312.753 kb on + strand at 312.753 kb on + strand at 312.753 kb on + strand at 312.754 kb on - strand at 312.754 kb on - strand at 312.754 kb on - strand at 313.064 kb on + strand, within OKGIIK_01405 at 313.064 kb on + strand, within OKGIIK_01405 at 313.065 kb on - strand, within OKGIIK_01405 at 313.271 kb on + strand, within OKGIIK_01405 at 313.272 kb on - strand, within OKGIIK_01405 at 313.272 kb on - strand, within OKGIIK_01405 at 313.272 kb on - strand, within OKGIIK_01405 at 313.272 kb on - strand, within OKGIIK_01405 at 313.272 kb on - strand, within OKGIIK_01405 at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.510 kb on + strand at 313.511 kb on - strand at 313.511 kb on - strand at 313.511 kb on - strand at 313.511 kb on - strand at 313.511 kb on - strand at 313.511 kb on - strand at 313.511 kb on - strand at 313.511 kb on - strand at 313.511 kb on - strand at 313.639 kb on + strand, within OKGIIK_01410 at 313.639 kb on + strand, within OKGIIK_01410 at 313.639 kb on + strand, within OKGIIK_01410 at 313.640 kb on - strand, within OKGIIK_01410 at 313.640 kb on - strand, within OKGIIK_01410 at 314.086 kb on + strand, within OKGIIK_01410 at 314.086 kb on + strand, within OKGIIK_01410 at 314.086 kb on + strand, within OKGIIK_01410 at 314.086 kb on + strand, within OKGIIK_01410 at 314.087 kb on - strand, within OKGIIK_01410 at 314.087 kb on - strand, within OKGIIK_01410 at 314.087 kb on - strand, within OKGIIK_01410 at 314.087 kb on - strand, within OKGIIK_01410 at 314.149 kb on + strand, within OKGIIK_01410 at 314.149 kb on + strand, within OKGIIK_01410 at 314.149 kb on + strand, within OKGIIK_01410 at 314.149 kb on + strand, within OKGIIK_01410 at 314.149 kb on + strand, within OKGIIK_01410 at 314.150 kb on - strand, within OKGIIK_01410 at 314.150 kb on - strand, within OKGIIK_01410 at 314.150 kb on - strand, within OKGIIK_01410 at 314.150 kb on - strand, within OKGIIK_01410 at 314.150 kb on - strand, within OKGIIK_01410 at 314.150 kb on - strand, within OKGIIK_01410 at 314.150 kb on - strand, within OKGIIK_01410 at 314.200 kb on + strand, within OKGIIK_01410 at 314.200 kb on + strand, within OKGIIK_01410 at 314.200 kb on + strand, within OKGIIK_01410 at 314.454 kb on + strand at 314.499 kb on + strand at 314.499 kb on + strand at 314.499 kb on + strand at 314.499 kb on + strand at 314.499 kb on + strand at 314.580 kb on + strand, within OKGIIK_01415 at 314.581 kb on - strand, within OKGIIK_01415 at 314.737 kb on + strand at 314.737 kb on + strand at 314.737 kb on + strand at 314.737 kb on + strand at 314.737 kb on + strand at 314.738 kb on - strand at 314.738 kb on - strand at 314.857 kb on + strand at 314.857 kb on + strand at 314.858 kb on - strand at 315.175 kb on + strand, within OKGIIK_01420 at 315.175 kb on + strand, within OKGIIK_01420 at 315.175 kb on + strand, within OKGIIK_01420 at 315.175 kb on + strand, within OKGIIK_01420 at 315.175 kb on + strand, within OKGIIK_01420 at 315.176 kb on - strand, within OKGIIK_01420 at 315.176 kb on - strand, within OKGIIK_01420 at 315.176 kb on - strand, within OKGIIK_01420 at 315.223 kb on + strand, within OKGIIK_01420 at 315.223 kb on + strand, within OKGIIK_01420 at 315.223 kb on + strand, within OKGIIK_01420 at 315.223 kb on + strand, within OKGIIK_01420 at 315.223 kb on + strand, within OKGIIK_01420 at 315.223 kb on + strand, within OKGIIK_01420 at 315.224 kb on - strand, within OKGIIK_01420 at 315.224 kb on - strand, within OKGIIK_01420 at 315.325 kb on + strand, within OKGIIK_01420 at 315.326 kb on - strand, within OKGIIK_01420 at 315.326 kb on - strand, within OKGIIK_01420 at 315.326 kb on - strand, within OKGIIK_01420 at 315.326 kb on - strand, within OKGIIK_01420 at 315.332 kb on + strand, within OKGIIK_01420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 312,624 + fadE OKGIIK_01400 0.88 -2.0 312,624 + fadE OKGIIK_01400 0.88 -0.2 312,625 - fadE OKGIIK_01400 0.88 +0.8 312,625 - fadE OKGIIK_01400 0.88 -0.1 312,625 - fadE OKGIIK_01400 0.88 -1.6 312,625 - fadE OKGIIK_01400 0.88 -0.3 312,625 - fadE OKGIIK_01400 0.88 +0.4 312,625 - fadE OKGIIK_01400 0.88 +1.2 312,753 + +0.7 312,753 + +0.0 312,753 + +1.2 312,753 + -2.2 312,753 + -0.1 312,753 + -0.2 312,753 + -1.2 312,754 - +1.2 312,754 - +0.7 312,754 - -0.1 313,064 + OKGIIK_01405 0.20 +0.0 313,064 + OKGIIK_01405 0.20 -0.3 313,065 - OKGIIK_01405 0.20 -0.4 313,271 + OKGIIK_01405 0.63 +0.7 313,272 - OKGIIK_01405 0.64 +0.4 313,272 - OKGIIK_01405 0.64 -1.2 313,272 - OKGIIK_01405 0.64 +0.3 313,272 - OKGIIK_01405 0.64 -0.8 313,272 - OKGIIK_01405 0.64 -1.3 313,510 + +0.2 313,510 + -0.6 313,510 + -0.2 313,510 + -0.6 313,510 + -1.1 313,510 + -1.2 313,510 + -0.2 313,510 + +2.1 313,510 + +0.1 313,510 + -1.4 313,510 + -2.0 313,510 + -0.6 313,510 + +0.1 313,510 + +0.6 313,510 + +0.9 313,511 - -2.0 313,511 - +1.3 313,511 - +0.4 313,511 - +0.7 313,511 - +1.4 313,511 - +2.1 313,511 - +0.2 313,511 - +0.1 313,511 - -3.3 313,639 + OKGIIK_01410 0.17 +1.4 313,639 + OKGIIK_01410 0.17 -1.7 313,639 + OKGIIK_01410 0.17 -0.1 313,640 - OKGIIK_01410 0.18 +0.1 313,640 - OKGIIK_01410 0.18 -1.4 314,086 + OKGIIK_01410 0.62 +0.8 314,086 + OKGIIK_01410 0.62 -1.2 314,086 + OKGIIK_01410 0.62 -0.9 314,086 + OKGIIK_01410 0.62 +0.7 314,087 - OKGIIK_01410 0.62 -1.4 314,087 - OKGIIK_01410 0.62 -0.4 314,087 - OKGIIK_01410 0.62 -0.7 314,087 - OKGIIK_01410 0.62 -1.6 314,149 + OKGIIK_01410 0.68 -0.0 314,149 + OKGIIK_01410 0.68 -0.5 314,149 + OKGIIK_01410 0.68 -0.2 314,149 + OKGIIK_01410 0.68 -2.5 314,149 + OKGIIK_01410 0.68 +0.1 314,150 - OKGIIK_01410 0.68 -0.9 314,150 - OKGIIK_01410 0.68 -0.9 314,150 - OKGIIK_01410 0.68 -0.6 314,150 - OKGIIK_01410 0.68 +0.3 314,150 - OKGIIK_01410 0.68 -2.4 314,150 - OKGIIK_01410 0.68 +1.4 314,150 - OKGIIK_01410 0.68 -1.2 314,200 + OKGIIK_01410 0.73 +0.5 314,200 + OKGIIK_01410 0.73 +1.4 314,200 + OKGIIK_01410 0.73 +0.3 314,454 + +0.1 314,499 + +1.1 314,499 + +0.2 314,499 + +0.0 314,499 + +0.2 314,499 + +1.7 314,580 + OKGIIK_01415 0.65 +1.4 314,581 - OKGIIK_01415 0.66 +1.6 314,737 + -0.6 314,737 + +0.8 314,737 + -2.7 314,737 + -0.6 314,737 + +0.4 314,738 - -0.6 314,738 - -1.4 314,857 + -0.5 314,857 + -0.0 314,858 - -0.6 315,175 + OKGIIK_01420 0.14 +0.4 315,175 + OKGIIK_01420 0.14 -0.6 315,175 + OKGIIK_01420 0.14 -0.7 315,175 + OKGIIK_01420 0.14 -0.4 315,175 + OKGIIK_01420 0.14 +0.7 315,176 - OKGIIK_01420 0.14 -0.9 315,176 - OKGIIK_01420 0.14 -0.8 315,176 - OKGIIK_01420 0.14 +0.4 315,223 + OKGIIK_01420 0.15 -1.0 315,223 + OKGIIK_01420 0.15 -1.3 315,223 + OKGIIK_01420 0.15 -0.3 315,223 + OKGIIK_01420 0.15 -0.2 315,223 + OKGIIK_01420 0.15 -2.3 315,223 + OKGIIK_01420 0.15 +0.2 315,224 - OKGIIK_01420 0.15 -0.2 315,224 - OKGIIK_01420 0.15 +1.4 315,325 + OKGIIK_01420 0.18 +0.4 315,326 - OKGIIK_01420 0.18 -1.2 315,326 - OKGIIK_01420 0.18 +0.7 315,326 - OKGIIK_01420 0.18 -0.7 315,326 - OKGIIK_01420 0.18 -1.6 315,332 + OKGIIK_01420 0.18 -0.9
Or see this region's nucleotide sequence