Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_12565 and OKGIIK_12570 are separated by 165 nucleotides OKGIIK_12570 and rpoE are separated by 122 nucleotides rpoE and OKGIIK_12580 overlap by 4 nucleotides OKGIIK_12580 and dapB are separated by 147 nucleotides
OKGIIK_12565: OKGIIK_12565 - hypothetical protein, at 2,707,916 to 2,708,203
_12565
OKGIIK_12570: OKGIIK_12570 - DUF6249 domain-containing protein, at 2,708,369 to 2,708,695
_12570
OKGIIK_12575: rpoE - RNA polymerase subunit sigma-24, at 2,708,818 to 2,709,399
rpoE
OKGIIK_12580: OKGIIK_12580 - hypothetical protein, at 2,709,396 to 2,709,875
_12580
OKGIIK_12585: dapB - 4-hydroxy-tetrahydrodipicolinate reductase, at 2,710,023 to 2,710,823
dapB
Position (kb)
2708
2709
2710 Strain fitness (log2 ratio)
-2
-1
0
1 at 2707.828 kb on + strand at 2707.829 kb on - strand at 2707.829 kb on - strand at 2707.829 kb on - strand at 2707.829 kb on - strand at 2707.896 kb on + strand at 2707.896 kb on + strand at 2707.896 kb on + strand at 2707.896 kb on + strand at 2707.896 kb on + strand at 2707.896 kb on + strand at 2707.896 kb on + strand at 2707.896 kb on + strand at 2707.896 kb on + strand at 2707.896 kb on + strand at 2707.897 kb on - strand at 2707.897 kb on - strand at 2707.897 kb on - strand at 2707.897 kb on - strand at 2707.897 kb on - strand at 2707.897 kb on - strand at 2707.897 kb on - strand at 2707.900 kb on + strand at 2707.900 kb on + strand at 2707.900 kb on + strand at 2707.900 kb on + strand at 2707.900 kb on + strand at 2707.900 kb on + strand at 2707.900 kb on + strand at 2707.900 kb on + strand at 2707.901 kb on - strand at 2707.901 kb on - strand at 2707.901 kb on - strand at 2708.105 kb on - strand, within OKGIIK_12565 at 2708.105 kb on - strand, within OKGIIK_12565 at 2708.105 kb on - strand, within OKGIIK_12565 at 2708.105 kb on - strand, within OKGIIK_12565 at 2708.105 kb on - strand, within OKGIIK_12565 at 2708.105 kb on - strand, within OKGIIK_12565 at 2708.371 kb on - strand at 2708.371 kb on - strand at 2708.631 kb on - strand, within OKGIIK_12570 at 2708.631 kb on - strand, within OKGIIK_12570 at 2708.747 kb on + strand at 2708.747 kb on + strand at 2708.748 kb on - strand at 2708.748 kb on - strand at 2708.748 kb on - strand at 2708.748 kb on - strand at 2708.748 kb on - strand at 2708.805 kb on + strand at 2709.017 kb on - strand, within rpoE at 2709.023 kb on - strand, within rpoE at 2709.061 kb on + strand, within rpoE at 2709.062 kb on - strand, within rpoE at 2709.062 kb on - strand, within rpoE at 2709.062 kb on - strand, within rpoE at 2709.134 kb on - strand, within rpoE at 2709.134 kb on - strand, within rpoE at 2709.134 kb on - strand, within rpoE at 2709.261 kb on - strand, within rpoE at 2709.262 kb on + strand, within rpoE at 2709.262 kb on + strand, within rpoE at 2709.262 kb on + strand, within rpoE at 2709.262 kb on + strand, within rpoE at 2709.262 kb on + strand, within rpoE at 2709.262 kb on + strand, within rpoE at 2709.262 kb on + strand, within rpoE at 2709.262 kb on + strand, within rpoE at 2709.262 kb on + strand, within rpoE at 2709.262 kb on + strand, within rpoE at 2709.262 kb on + strand, within rpoE at 2709.263 kb on - strand, within rpoE at 2709.263 kb on - strand, within rpoE at 2709.263 kb on - strand, within rpoE at 2709.263 kb on - strand, within rpoE at 2709.263 kb on - strand, within rpoE at 2709.263 kb on - strand, within rpoE at 2709.263 kb on - strand, within rpoE at 2709.263 kb on - strand, within rpoE at 2709.266 kb on - strand, within rpoE at 2709.266 kb on - strand, within rpoE at 2709.266 kb on - strand, within rpoE at 2709.266 kb on - strand, within rpoE at 2709.266 kb on - strand, within rpoE at 2709.266 kb on - strand, within rpoE at 2709.266 kb on - strand, within rpoE at 2709.266 kb on - strand, within rpoE at 2709.266 kb on - strand, within rpoE at 2709.718 kb on + strand, within OKGIIK_12580 at 2709.718 kb on + strand, within OKGIIK_12580 at 2709.726 kb on + strand, within OKGIIK_12580 at 2709.726 kb on + strand, within OKGIIK_12580 at 2709.726 kb on + strand, within OKGIIK_12580 at 2709.726 kb on + strand, within OKGIIK_12580 at 2709.727 kb on - strand, within OKGIIK_12580 at 2709.879 kb on - strand at 2710.024 kb on + strand at 2710.024 kb on + strand at 2710.024 kb on + strand at 2710.024 kb on + strand at 2710.024 kb on + strand at 2710.024 kb on + strand at 2710.024 kb on + strand at 2710.025 kb on - strand at 2710.025 kb on - strand at 2710.025 kb on - strand at 2710.025 kb on - strand at 2710.025 kb on - strand at 2710.108 kb on + strand, within dapB at 2710.108 kb on + strand, within dapB at 2710.108 kb on + strand, within dapB at 2710.108 kb on + strand, within dapB at 2710.109 kb on - strand, within dapB at 2710.188 kb on + strand, within dapB at 2710.188 kb on + strand, within dapB at 2710.188 kb on + strand, within dapB at 2710.189 kb on - strand, within dapB at 2710.189 kb on - strand, within dapB at 2710.329 kb on + strand, within dapB at 2710.330 kb on - strand, within dapB at 2710.330 kb on - strand, within dapB at 2710.330 kb on - strand, within dapB at 2710.330 kb on - strand, within dapB at 2710.330 kb on - strand, within dapB at 2710.330 kb on - strand, within dapB at 2710.363 kb on + strand, within dapB at 2710.369 kb on + strand, within dapB at 2710.369 kb on + strand, within dapB at 2710.369 kb on + strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 2,707,828 + -0.9 2,707,829 - -1.4 2,707,829 - -2.0 2,707,829 - -1.8 2,707,829 - -0.7 2,707,896 + -0.7 2,707,896 + +1.1 2,707,896 + -0.0 2,707,896 + -1.9 2,707,896 + -0.2 2,707,896 + +1.4 2,707,896 + +1.2 2,707,896 + -2.1 2,707,896 + +0.2 2,707,896 + -1.1 2,707,897 - +0.9 2,707,897 - -0.8 2,707,897 - -0.3 2,707,897 - +0.1 2,707,897 - -0.7 2,707,897 - +0.3 2,707,897 - +1.3 2,707,900 + +0.7 2,707,900 + +1.3 2,707,900 + +1.3 2,707,900 + +0.8 2,707,900 + -1.4 2,707,900 + +0.0 2,707,900 + -1.1 2,707,900 + -1.2 2,707,901 - -1.2 2,707,901 - -0.7 2,707,901 - +0.2 2,708,105 - OKGIIK_12565 0.66 -0.9 2,708,105 - OKGIIK_12565 0.66 +0.9 2,708,105 - OKGIIK_12565 0.66 +1.7 2,708,105 - OKGIIK_12565 0.66 +0.3 2,708,105 - OKGIIK_12565 0.66 -2.7 2,708,105 - OKGIIK_12565 0.66 +1.3 2,708,371 - -0.2 2,708,371 - +0.2 2,708,631 - OKGIIK_12570 0.80 -0.0 2,708,631 - OKGIIK_12570 0.80 -1.3 2,708,747 + -1.7 2,708,747 + -1.9 2,708,748 - +0.8 2,708,748 - +0.8 2,708,748 - -0.1 2,708,748 - -0.1 2,708,748 - -0.1 2,708,805 + -0.0 2,709,017 - rpoE OKGIIK_12575 0.34 -0.3 2,709,023 - rpoE OKGIIK_12575 0.35 -1.3 2,709,061 + rpoE OKGIIK_12575 0.42 -0.3 2,709,062 - rpoE OKGIIK_12575 0.42 -0.8 2,709,062 - rpoE OKGIIK_12575 0.42 -0.3 2,709,062 - rpoE OKGIIK_12575 0.42 +0.7 2,709,134 - rpoE OKGIIK_12575 0.54 -0.3 2,709,134 - rpoE OKGIIK_12575 0.54 +1.5 2,709,134 - rpoE OKGIIK_12575 0.54 -0.5 2,709,261 - rpoE OKGIIK_12575 0.76 +0.2 2,709,262 + rpoE OKGIIK_12575 0.76 -0.1 2,709,262 + rpoE OKGIIK_12575 0.76 +0.6 2,709,262 + rpoE OKGIIK_12575 0.76 +1.1 2,709,262 + rpoE OKGIIK_12575 0.76 -1.7 2,709,262 + rpoE OKGIIK_12575 0.76 -0.9 2,709,262 + rpoE OKGIIK_12575 0.76 -0.1 2,709,262 + rpoE OKGIIK_12575 0.76 -0.2 2,709,262 + rpoE OKGIIK_12575 0.76 +1.3 2,709,262 + rpoE OKGIIK_12575 0.76 +0.7 2,709,262 + rpoE OKGIIK_12575 0.76 +0.4 2,709,262 + rpoE OKGIIK_12575 0.76 -0.2 2,709,263 - rpoE OKGIIK_12575 0.76 +0.3 2,709,263 - rpoE OKGIIK_12575 0.76 +0.3 2,709,263 - rpoE OKGIIK_12575 0.76 +0.9 2,709,263 - rpoE OKGIIK_12575 0.76 -0.8 2,709,263 - rpoE OKGIIK_12575 0.76 +0.9 2,709,263 - rpoE OKGIIK_12575 0.76 -0.7 2,709,263 - rpoE OKGIIK_12575 0.76 +0.7 2,709,263 - rpoE OKGIIK_12575 0.76 +0.3 2,709,266 - rpoE OKGIIK_12575 0.77 +0.4 2,709,266 - rpoE OKGIIK_12575 0.77 -1.8 2,709,266 - rpoE OKGIIK_12575 0.77 +0.4 2,709,266 - rpoE OKGIIK_12575 0.77 +0.7 2,709,266 - rpoE OKGIIK_12575 0.77 -1.1 2,709,266 - rpoE OKGIIK_12575 0.77 -1.6 2,709,266 - rpoE OKGIIK_12575 0.77 +0.1 2,709,266 - rpoE OKGIIK_12575 0.77 -0.2 2,709,266 - rpoE OKGIIK_12575 0.77 -0.5 2,709,718 + OKGIIK_12580 0.67 +0.2 2,709,718 + OKGIIK_12580 0.67 +0.4 2,709,726 + OKGIIK_12580 0.69 +0.3 2,709,726 + OKGIIK_12580 0.69 +0.7 2,709,726 + OKGIIK_12580 0.69 -0.5 2,709,726 + OKGIIK_12580 0.69 +0.7 2,709,727 - OKGIIK_12580 0.69 +0.6 2,709,879 - +0.6 2,710,024 + -0.0 2,710,024 + -1.9 2,710,024 + -2.4 2,710,024 + +0.1 2,710,024 + -2.4 2,710,024 + +0.0 2,710,024 + -2.1 2,710,025 - -1.3 2,710,025 - -0.6 2,710,025 - +0.3 2,710,025 - -0.7 2,710,025 - +0.3 2,710,108 + dapB OKGIIK_12585 0.11 +0.5 2,710,108 + dapB OKGIIK_12585 0.11 -1.7 2,710,108 + dapB OKGIIK_12585 0.11 -0.6 2,710,108 + dapB OKGIIK_12585 0.11 +1.1 2,710,109 - dapB OKGIIK_12585 0.11 -1.9 2,710,188 + dapB OKGIIK_12585 0.21 -1.2 2,710,188 + dapB OKGIIK_12585 0.21 +1.4 2,710,188 + dapB OKGIIK_12585 0.21 +0.2 2,710,189 - dapB OKGIIK_12585 0.21 +0.4 2,710,189 - dapB OKGIIK_12585 0.21 +1.7 2,710,329 + dapB OKGIIK_12585 0.38 -2.0 2,710,330 - dapB OKGIIK_12585 0.38 -2.1 2,710,330 - dapB OKGIIK_12585 0.38 +0.7 2,710,330 - dapB OKGIIK_12585 0.38 -0.2 2,710,330 - dapB OKGIIK_12585 0.38 -1.7 2,710,330 - dapB OKGIIK_12585 0.38 +0.3 2,710,330 - dapB OKGIIK_12585 0.38 -0.9 2,710,363 + dapB OKGIIK_12585 0.42 -0.5 2,710,369 + dapB OKGIIK_12585 0.43 -1.9 2,710,369 + dapB OKGIIK_12585 0.43 -1.1 2,710,369 + dapB OKGIIK_12585 0.43 -0.9 2,710,370 - dapB OKGIIK_12585 0.43 +0.4 2,710,370 - dapB OKGIIK_12585 0.43 -1.7 2,710,370 - dapB OKGIIK_12585 0.43 +0.7 2,710,370 - dapB OKGIIK_12585 0.43 -0.9 2,710,370 - dapB OKGIIK_12585 0.43 -0.5 2,710,370 - dapB OKGIIK_12585 0.43 +0.9 2,710,370 - dapB OKGIIK_12585 0.43 -0.5 2,710,370 - dapB OKGIIK_12585 0.43 -0.6
Or see this region's nucleotide sequence