Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ssuD and rIO1 are separated by 84 nucleotides rIO1 and fieF are separated by 22 nucleotides fieF and slp are separated by 65 nucleotides slp and rlmJ are separated by 39 nucleotides
OKGIIK_04540: ssuD - LLM class flavin-dependent oxidoreductase, at 993,819 to 994,817
ssuD
OKGIIK_04545: rIO1 - serine protein kinase RIO, at 994,902 to 995,759
rIO1
OKGIIK_04550: fieF - cation efflux family transporter, at 995,782 to 996,693
fieF
OKGIIK_04555: slp - Outer membrane lipoprotein Slp family, at 996,759 to 997,274
slp
OKGIIK_04560: rlmJ - 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ, at 997,314 to 998,228
rlmJ
Position (kb) 995
996
997 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 994.861 kb on + strand at 994.861 kb on + strand at 994.862 kb on - strand at 994.862 kb on - strand at 994.862 kb on - strand at 994.862 kb on - strand at 994.871 kb on + strand at 994.871 kb on + strand at 994.872 kb on - strand at 994.872 kb on - strand at 994.872 kb on - strand at 994.995 kb on + strand, within rIO1 at 994.995 kb on + strand, within rIO1 at 994.995 kb on + strand, within rIO1 at 994.996 kb on - strand, within rIO1 at 994.996 kb on - strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.079 kb on + strand, within rIO1 at 995.079 kb on + strand, within rIO1 at 995.080 kb on - strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.197 kb on - strand, within rIO1 at 995.197 kb on - strand, within rIO1 at 995.197 kb on - strand, within rIO1 at 995.197 kb on - strand, within rIO1 at 995.197 kb on - strand, within rIO1 at 995.235 kb on + strand, within rIO1 at 995.236 kb on - strand, within rIO1 at 995.236 kb on - strand, within rIO1 at 995.236 kb on - strand, within rIO1 at 995.241 kb on + strand, within rIO1 at 995.241 kb on + strand, within rIO1 at 995.241 kb on + strand, within rIO1 at 995.242 kb on - strand, within rIO1 at 995.343 kb on + strand, within rIO1 at 995.344 kb on - strand, within rIO1 at 995.415 kb on + strand, within rIO1 at 995.415 kb on + strand, within rIO1 at 995.415 kb on + strand, within rIO1 at 995.415 kb on + strand, within rIO1 at 995.415 kb on + strand, within rIO1 at 995.416 kb on - strand, within rIO1 at 995.416 kb on - strand, within rIO1 at 995.416 kb on - strand, within rIO1 at 995.416 kb on - strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.531 kb on + strand, within rIO1 at 995.531 kb on + strand, within rIO1 at 995.531 kb on + strand, within rIO1 at 995.531 kb on + strand, within rIO1 at 995.532 kb on - strand, within rIO1 at 995.532 kb on - strand, within rIO1 at 995.574 kb on + strand, within rIO1 at 995.598 kb on + strand, within rIO1 at 995.598 kb on + strand, within rIO1 at 995.598 kb on + strand, within rIO1 at 995.598 kb on + strand, within rIO1 at 995.598 kb on + strand, within rIO1 at 995.599 kb on - strand, within rIO1 at 995.599 kb on - strand, within rIO1 at 995.599 kb on - strand, within rIO1 at 995.599 kb on - strand, within rIO1 at 996.306 kb on + strand, within fieF at 996.306 kb on + strand, within fieF at 996.306 kb on + strand, within fieF at 996.306 kb on + strand, within fieF at 996.306 kb on + strand, within fieF at 996.306 kb on + strand, within fieF at 996.306 kb on + strand, within fieF at 996.306 kb on + strand, within fieF at 996.306 kb on + strand, within fieF at 996.306 kb on + strand, within fieF at 996.306 kb on + strand, within fieF at 996.306 kb on + strand, within fieF at 996.307 kb on - strand, within fieF at 996.307 kb on - strand, within fieF at 996.307 kb on - strand, within fieF at 996.307 kb on - strand, within fieF at 996.307 kb on - strand, within fieF at 996.307 kb on - strand, within fieF at 996.398 kb on + strand, within fieF at 996.398 kb on + strand, within fieF at 996.398 kb on + strand, within fieF at 996.398 kb on + strand, within fieF at 996.398 kb on + strand, within fieF at 996.399 kb on - strand, within fieF at 996.399 kb on - strand, within fieF at 996.399 kb on - strand, within fieF at 996.399 kb on - strand, within fieF at 996.399 kb on - strand, within fieF at 996.399 kb on - strand, within fieF at 996.399 kb on - strand, within fieF at 996.458 kb on + strand, within fieF at 996.458 kb on + strand, within fieF at 996.458 kb on + strand, within fieF at 996.458 kb on + strand, within fieF at 996.458 kb on + strand, within fieF at 996.458 kb on + strand, within fieF at 996.458 kb on + strand, within fieF at 996.458 kb on + strand, within fieF at 996.458 kb on + strand, within fieF at 996.458 kb on + strand, within fieF at 996.458 kb on + strand, within fieF at 996.458 kb on + strand, within fieF at 996.458 kb on + strand, within fieF at 996.459 kb on - strand, within fieF at 996.459 kb on - strand, within fieF at 996.459 kb on - strand, within fieF at 996.459 kb on - strand, within fieF at 996.459 kb on - strand, within fieF at 996.729 kb on + strand at 996.871 kb on + strand, within slp at 996.871 kb on + strand, within slp at 996.871 kb on + strand, within slp at 996.871 kb on + strand, within slp at 996.871 kb on + strand, within slp at 996.871 kb on + strand, within slp at 996.871 kb on + strand, within slp at 996.871 kb on + strand, within slp at 996.871 kb on + strand, within slp at 996.872 kb on - strand, within slp at 996.872 kb on - strand, within slp at 996.872 kb on - strand, within slp at 996.872 kb on - strand, within slp at 996.872 kb on - strand, within slp at 996.872 kb on - strand, within slp at 996.872 kb on - strand, within slp at 996.872 kb on - strand, within slp at 996.872 kb on - strand, within slp at 996.946 kb on + strand, within slp at 996.946 kb on + strand, within slp at 996.947 kb on - strand, within slp at 996.964 kb on + strand, within slp at 996.964 kb on + strand, within slp at 996.964 kb on + strand, within slp at 996.965 kb on - strand, within slp at 997.186 kb on + strand, within slp at 997.186 kb on + strand, within slp at 997.186 kb on + strand, within slp at 997.186 kb on + strand, within slp at 997.186 kb on + strand, within slp at 997.186 kb on + strand, within slp at 997.186 kb on + strand, within slp at 997.186 kb on - strand, within slp at 997.187 kb on - strand, within slp at 997.187 kb on - strand, within slp at 997.187 kb on - strand, within slp at 997.187 kb on - strand, within slp at 997.187 kb on - strand, within slp at 997.187 kb on - strand, within slp at 997.315 kb on + strand at 997.316 kb on - strand at 997.640 kb on - strand, within rlmJ at 997.651 kb on + strand, within rlmJ at 997.652 kb on - strand, within rlmJ
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 994,861 + -0.2 994,861 + -1.3 994,862 - -1.2 994,862 - +0.1 994,862 - +0.4 994,862 - -0.7 994,871 + -0.5 994,871 + -2.3 994,872 - -0.5 994,872 - +0.4 994,872 - -0.9 994,995 + rIO1 OKGIIK_04545 0.11 -0.0 994,995 + rIO1 OKGIIK_04545 0.11 +0.8 994,995 + rIO1 OKGIIK_04545 0.11 +0.4 994,996 - rIO1 OKGIIK_04545 0.11 +0.3 994,996 - rIO1 OKGIIK_04545 0.11 -0.2 995,037 + rIO1 OKGIIK_04545 0.16 -1.1 995,037 + rIO1 OKGIIK_04545 0.16 -0.1 995,037 + rIO1 OKGIIK_04545 0.16 +1.2 995,037 + rIO1 OKGIIK_04545 0.16 -0.6 995,037 + rIO1 OKGIIK_04545 0.16 +0.8 995,037 + rIO1 OKGIIK_04545 0.16 -1.0 995,037 + rIO1 OKGIIK_04545 0.16 -0.6 995,038 - rIO1 OKGIIK_04545 0.16 +0.2 995,038 - rIO1 OKGIIK_04545 0.16 +0.4 995,038 - rIO1 OKGIIK_04545 0.16 -0.2 995,038 - rIO1 OKGIIK_04545 0.16 +1.3 995,038 - rIO1 OKGIIK_04545 0.16 +0.1 995,038 - rIO1 OKGIIK_04545 0.16 -1.2 995,038 - rIO1 OKGIIK_04545 0.16 -0.2 995,079 + rIO1 OKGIIK_04545 0.21 -0.8 995,079 + rIO1 OKGIIK_04545 0.21 +1.0 995,080 - rIO1 OKGIIK_04545 0.21 -0.1 995,196 + rIO1 OKGIIK_04545 0.34 +0.9 995,196 + rIO1 OKGIIK_04545 0.34 +0.3 995,196 + rIO1 OKGIIK_04545 0.34 +0.1 995,196 + rIO1 OKGIIK_04545 0.34 +0.8 995,196 + rIO1 OKGIIK_04545 0.34 -0.1 995,196 + rIO1 OKGIIK_04545 0.34 +0.4 995,196 + rIO1 OKGIIK_04545 0.34 +1.2 995,197 - rIO1 OKGIIK_04545 0.34 -1.0 995,197 - rIO1 OKGIIK_04545 0.34 +1.6 995,197 - rIO1 OKGIIK_04545 0.34 +0.5 995,197 - rIO1 OKGIIK_04545 0.34 -0.2 995,197 - rIO1 OKGIIK_04545 0.34 -1.7 995,235 + rIO1 OKGIIK_04545 0.39 +1.0 995,236 - rIO1 OKGIIK_04545 0.39 -0.2 995,236 - rIO1 OKGIIK_04545 0.39 -0.5 995,236 - rIO1 OKGIIK_04545 0.39 -0.3 995,241 + rIO1 OKGIIK_04545 0.40 +0.9 995,241 + rIO1 OKGIIK_04545 0.40 +0.4 995,241 + rIO1 OKGIIK_04545 0.40 +0.2 995,242 - rIO1 OKGIIK_04545 0.40 +1.3 995,343 + rIO1 OKGIIK_04545 0.51 +0.6 995,344 - rIO1 OKGIIK_04545 0.52 +0.7 995,415 + rIO1 OKGIIK_04545 0.60 +0.1 995,415 + rIO1 OKGIIK_04545 0.60 -0.1 995,415 + rIO1 OKGIIK_04545 0.60 -1.5 995,415 + rIO1 OKGIIK_04545 0.60 -0.5 995,415 + rIO1 OKGIIK_04545 0.60 +1.1 995,416 - rIO1 OKGIIK_04545 0.60 +1.3 995,416 - rIO1 OKGIIK_04545 0.60 -1.2 995,416 - rIO1 OKGIIK_04545 0.60 +0.2 995,416 - rIO1 OKGIIK_04545 0.60 -0.9 995,525 + rIO1 OKGIIK_04545 0.73 -0.9 995,525 + rIO1 OKGIIK_04545 0.73 -0.2 995,525 + rIO1 OKGIIK_04545 0.73 +0.1 995,525 + rIO1 OKGIIK_04545 0.73 +0.1 995,525 + rIO1 OKGIIK_04545 0.73 +1.5 995,525 + rIO1 OKGIIK_04545 0.73 +0.2 995,525 + rIO1 OKGIIK_04545 0.73 -1.5 995,525 + rIO1 OKGIIK_04545 0.73 +0.4 995,526 - rIO1 OKGIIK_04545 0.73 -0.9 995,526 - rIO1 OKGIIK_04545 0.73 -0.5 995,526 - rIO1 OKGIIK_04545 0.73 +2.1 995,526 - rIO1 OKGIIK_04545 0.73 +0.6 995,526 - rIO1 OKGIIK_04545 0.73 +1.1 995,526 - rIO1 OKGIIK_04545 0.73 -0.9 995,526 - rIO1 OKGIIK_04545 0.73 +0.4 995,526 - rIO1 OKGIIK_04545 0.73 -0.3 995,526 - rIO1 OKGIIK_04545 0.73 +1.6 995,526 - rIO1 OKGIIK_04545 0.73 -0.1 995,526 - rIO1 OKGIIK_04545 0.73 +0.1 995,526 - rIO1 OKGIIK_04545 0.73 -0.1 995,526 - rIO1 OKGIIK_04545 0.73 +1.1 995,531 + rIO1 OKGIIK_04545 0.73 -1.2 995,531 + rIO1 OKGIIK_04545 0.73 +0.5 995,531 + rIO1 OKGIIK_04545 0.73 +0.4 995,531 + rIO1 OKGIIK_04545 0.73 +1.0 995,532 - rIO1 OKGIIK_04545 0.73 +0.5 995,532 - rIO1 OKGIIK_04545 0.73 -1.5 995,574 + rIO1 OKGIIK_04545 0.78 -0.7 995,598 + rIO1 OKGIIK_04545 0.81 +0.5 995,598 + rIO1 OKGIIK_04545 0.81 +0.6 995,598 + rIO1 OKGIIK_04545 0.81 -0.9 995,598 + rIO1 OKGIIK_04545 0.81 -1.9 995,598 + rIO1 OKGIIK_04545 0.81 -0.8 995,599 - rIO1 OKGIIK_04545 0.81 +0.1 995,599 - rIO1 OKGIIK_04545 0.81 +0.1 995,599 - rIO1 OKGIIK_04545 0.81 -0.2 995,599 - rIO1 OKGIIK_04545 0.81 -1.5 996,306 + fieF OKGIIK_04550 0.57 -1.1 996,306 + fieF OKGIIK_04550 0.57 -0.1 996,306 + fieF OKGIIK_04550 0.57 +0.1 996,306 + fieF OKGIIK_04550 0.57 -1.0 996,306 + fieF OKGIIK_04550 0.57 -0.4 996,306 + fieF OKGIIK_04550 0.57 -2.0 996,306 + fieF OKGIIK_04550 0.57 -0.5 996,306 + fieF OKGIIK_04550 0.57 +0.9 996,306 + fieF OKGIIK_04550 0.57 -0.1 996,306 + fieF OKGIIK_04550 0.57 +1.1 996,306 + fieF OKGIIK_04550 0.57 +1.1 996,306 + fieF OKGIIK_04550 0.57 +0.9 996,307 - fieF OKGIIK_04550 0.58 -1.7 996,307 - fieF OKGIIK_04550 0.58 -0.0 996,307 - fieF OKGIIK_04550 0.58 +0.3 996,307 - fieF OKGIIK_04550 0.58 -0.8 996,307 - fieF OKGIIK_04550 0.58 +0.6 996,307 - fieF OKGIIK_04550 0.58 -1.3 996,398 + fieF OKGIIK_04550 0.68 +2.2 996,398 + fieF OKGIIK_04550 0.68 -0.5 996,398 + fieF OKGIIK_04550 0.68 -1.0 996,398 + fieF OKGIIK_04550 0.68 -0.5 996,398 + fieF OKGIIK_04550 0.68 -2.2 996,399 - fieF OKGIIK_04550 0.68 -0.1 996,399 - fieF OKGIIK_04550 0.68 -1.2 996,399 - fieF OKGIIK_04550 0.68 +0.4 996,399 - fieF OKGIIK_04550 0.68 -0.5 996,399 - fieF OKGIIK_04550 0.68 +0.7 996,399 - fieF OKGIIK_04550 0.68 +1.8 996,399 - fieF OKGIIK_04550 0.68 -0.7 996,458 + fieF OKGIIK_04550 0.74 -0.5 996,458 + fieF OKGIIK_04550 0.74 -0.3 996,458 + fieF OKGIIK_04550 0.74 -1.7 996,458 + fieF OKGIIK_04550 0.74 -0.5 996,458 + fieF OKGIIK_04550 0.74 -0.2 996,458 + fieF OKGIIK_04550 0.74 -0.1 996,458 + fieF OKGIIK_04550 0.74 +0.4 996,458 + fieF OKGIIK_04550 0.74 -2.2 996,458 + fieF OKGIIK_04550 0.74 -0.2 996,458 + fieF OKGIIK_04550 0.74 -0.7 996,458 + fieF OKGIIK_04550 0.74 +0.2 996,458 + fieF OKGIIK_04550 0.74 +0.1 996,458 + fieF OKGIIK_04550 0.74 +0.4 996,459 - fieF OKGIIK_04550 0.74 -1.0 996,459 - fieF OKGIIK_04550 0.74 +0.5 996,459 - fieF OKGIIK_04550 0.74 +0.7 996,459 - fieF OKGIIK_04550 0.74 -1.2 996,459 - fieF OKGIIK_04550 0.74 -0.5 996,729 + -0.5 996,871 + slp OKGIIK_04555 0.22 -2.0 996,871 + slp OKGIIK_04555 0.22 +0.5 996,871 + slp OKGIIK_04555 0.22 +0.1 996,871 + slp OKGIIK_04555 0.22 -1.7 996,871 + slp OKGIIK_04555 0.22 +0.0 996,871 + slp OKGIIK_04555 0.22 -0.9 996,871 + slp OKGIIK_04555 0.22 +0.1 996,871 + slp OKGIIK_04555 0.22 +0.5 996,871 + slp OKGIIK_04555 0.22 -0.7 996,872 - slp OKGIIK_04555 0.22 -1.5 996,872 - slp OKGIIK_04555 0.22 -2.0 996,872 - slp OKGIIK_04555 0.22 -0.5 996,872 - slp OKGIIK_04555 0.22 -0.8 996,872 - slp OKGIIK_04555 0.22 +0.7 996,872 - slp OKGIIK_04555 0.22 -0.4 996,872 - slp OKGIIK_04555 0.22 -0.3 996,872 - slp OKGIIK_04555 0.22 -0.1 996,872 - slp OKGIIK_04555 0.22 -0.1 996,946 + slp OKGIIK_04555 0.36 -1.2 996,946 + slp OKGIIK_04555 0.36 -0.5 996,947 - slp OKGIIK_04555 0.36 -0.1 996,964 + slp OKGIIK_04555 0.40 +0.7 996,964 + slp OKGIIK_04555 0.40 -0.5 996,964 + slp OKGIIK_04555 0.40 -3.0 996,965 - slp OKGIIK_04555 0.40 +1.1 997,186 + slp OKGIIK_04555 0.83 -2.2 997,186 + slp OKGIIK_04555 0.83 -0.2 997,186 + slp OKGIIK_04555 0.83 -0.6 997,186 + slp OKGIIK_04555 0.83 +0.4 997,186 + slp OKGIIK_04555 0.83 -0.3 997,186 + slp OKGIIK_04555 0.83 +0.6 997,186 + slp OKGIIK_04555 0.83 -0.9 997,186 - slp OKGIIK_04555 0.83 -1.2 997,187 - slp OKGIIK_04555 0.83 +0.5 997,187 - slp OKGIIK_04555 0.83 -0.3 997,187 - slp OKGIIK_04555 0.83 -0.2 997,187 - slp OKGIIK_04555 0.83 +1.5 997,187 - slp OKGIIK_04555 0.83 -1.4 997,187 - slp OKGIIK_04555 0.83 -1.5 997,315 + +0.3 997,316 - +1.6 997,640 - rlmJ OKGIIK_04560 0.36 -0.9 997,651 + rlmJ OKGIIK_04560 0.37 +0.6 997,652 - rlmJ OKGIIK_04560 0.37 -0.7
Or see this region's nucleotide sequence