Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_04530 and rsuA are separated by 99 nucleotides rsuA and ssuD are separated by 17 nucleotides ssuD and rIO1 are separated by 84 nucleotides rIO1 and fieF are separated by 22 nucleotides
OKGIIK_04530: OKGIIK_04530 - hypothetical protein, at 992,791 to 992,892
_04530
OKGIIK_04535: rsuA - RNA-binding protein, at 992,992 to 993,801
rsuA
OKGIIK_04540: ssuD - LLM class flavin-dependent oxidoreductase, at 993,819 to 994,817
ssuD
OKGIIK_04545: rIO1 - serine protein kinase RIO, at 994,902 to 995,759
rIO1
OKGIIK_04550: fieF - cation efflux family transporter, at 995,782 to 996,693
fieF
Position (kb) 993
994
995 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.928 kb on + strand at 992.929 kb on - strand at 992.929 kb on - strand at 992.929 kb on - strand at 992.929 kb on - strand at 992.929 kb on - strand at 992.929 kb on - strand at 992.929 kb on - strand at 992.929 kb on - strand at 992.933 kb on + strand at 992.933 kb on + strand at 992.934 kb on - strand at 992.934 kb on - strand at 992.934 kb on - strand at 992.934 kb on - strand at 992.947 kb on + strand at 992.947 kb on + strand at 992.947 kb on + strand at 992.947 kb on + strand at 992.947 kb on + strand at 992.947 kb on + strand at 992.947 kb on + strand at 992.948 kb on - strand at 992.948 kb on - strand at 992.948 kb on - strand at 993.018 kb on - strand at 993.018 kb on - strand at 993.494 kb on + strand, within rsuA at 993.494 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.641 kb on + strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.642 kb on - strand, within rsuA at 993.746 kb on + strand at 993.746 kb on + strand at 993.746 kb on + strand at 993.746 kb on + strand at 993.746 kb on + strand at 993.746 kb on + strand at 993.746 kb on + strand at 993.746 kb on + strand at 993.746 kb on + strand at 993.747 kb on - strand at 994.017 kb on - strand, within ssuD at 994.017 kb on - strand, within ssuD at 994.017 kb on - strand, within ssuD at 994.110 kb on + strand, within ssuD at 994.110 kb on + strand, within ssuD at 994.185 kb on + strand, within ssuD at 994.186 kb on - strand, within ssuD at 994.236 kb on + strand, within ssuD at 994.236 kb on + strand, within ssuD at 994.236 kb on + strand, within ssuD at 994.237 kb on - strand, within ssuD at 994.237 kb on - strand, within ssuD at 994.416 kb on + strand, within ssuD at 994.416 kb on + strand, within ssuD at 994.417 kb on - strand, within ssuD at 994.458 kb on + strand, within ssuD at 994.459 kb on - strand, within ssuD at 994.459 kb on - strand, within ssuD at 994.602 kb on + strand, within ssuD at 994.602 kb on + strand, within ssuD at 994.603 kb on - strand, within ssuD at 994.603 kb on - strand, within ssuD at 994.861 kb on + strand at 994.861 kb on + strand at 994.862 kb on - strand at 994.862 kb on - strand at 994.862 kb on - strand at 994.862 kb on - strand at 994.871 kb on + strand at 994.871 kb on + strand at 994.872 kb on - strand at 994.872 kb on - strand at 994.872 kb on - strand at 994.995 kb on + strand, within rIO1 at 994.995 kb on + strand, within rIO1 at 994.995 kb on + strand, within rIO1 at 994.996 kb on - strand, within rIO1 at 994.996 kb on - strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.037 kb on + strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.038 kb on - strand, within rIO1 at 995.079 kb on + strand, within rIO1 at 995.079 kb on + strand, within rIO1 at 995.080 kb on - strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.196 kb on + strand, within rIO1 at 995.197 kb on - strand, within rIO1 at 995.197 kb on - strand, within rIO1 at 995.197 kb on - strand, within rIO1 at 995.197 kb on - strand, within rIO1 at 995.197 kb on - strand, within rIO1 at 995.235 kb on + strand, within rIO1 at 995.236 kb on - strand, within rIO1 at 995.236 kb on - strand, within rIO1 at 995.236 kb on - strand, within rIO1 at 995.241 kb on + strand, within rIO1 at 995.241 kb on + strand, within rIO1 at 995.241 kb on + strand, within rIO1 at 995.242 kb on - strand, within rIO1 at 995.343 kb on + strand, within rIO1 at 995.344 kb on - strand, within rIO1 at 995.415 kb on + strand, within rIO1 at 995.415 kb on + strand, within rIO1 at 995.415 kb on + strand, within rIO1 at 995.415 kb on + strand, within rIO1 at 995.415 kb on + strand, within rIO1 at 995.416 kb on - strand, within rIO1 at 995.416 kb on - strand, within rIO1 at 995.416 kb on - strand, within rIO1 at 995.416 kb on - strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.525 kb on + strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.526 kb on - strand, within rIO1 at 995.531 kb on + strand, within rIO1 at 995.531 kb on + strand, within rIO1 at 995.531 kb on + strand, within rIO1 at 995.531 kb on + strand, within rIO1 at 995.532 kb on - strand, within rIO1 at 995.532 kb on - strand, within rIO1 at 995.574 kb on + strand, within rIO1 at 995.598 kb on + strand, within rIO1 at 995.598 kb on + strand, within rIO1 at 995.598 kb on + strand, within rIO1 at 995.598 kb on + strand, within rIO1 at 995.598 kb on + strand, within rIO1 at 995.599 kb on - strand, within rIO1 at 995.599 kb on - strand, within rIO1 at 995.599 kb on - strand, within rIO1 at 995.599 kb on - strand, within rIO1
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 992,928 + -1.5 992,928 + +0.6 992,928 + -0.5 992,928 + +1.1 992,928 + -0.7 992,928 + -0.5 992,928 + +0.5 992,928 + +0.5 992,928 + -2.6 992,928 + -0.3 992,928 + +0.2 992,928 + +0.6 992,928 + +0.4 992,928 + -0.9 992,928 + -0.9 992,928 + -0.3 992,928 + +0.4 992,928 + +0.9 992,929 - +0.6 992,929 - -1.0 992,929 - +0.8 992,929 - -2.1 992,929 - -0.4 992,929 - -0.8 992,929 - +0.8 992,929 - +0.6 992,933 + +0.8 992,933 + -1.0 992,934 - +1.3 992,934 - +0.2 992,934 - +0.6 992,934 - +0.1 992,947 + +0.2 992,947 + -1.1 992,947 + -1.3 992,947 + -0.9 992,947 + +1.0 992,947 + -0.9 992,947 + -0.9 992,948 - -0.4 992,948 - +0.8 992,948 - +0.9 993,018 - +1.8 993,018 - -3.2 993,494 + rsuA OKGIIK_04535 0.62 -2.3 993,494 + rsuA OKGIIK_04535 0.62 -0.9 993,641 + rsuA OKGIIK_04535 0.80 +1.3 993,641 + rsuA OKGIIK_04535 0.80 -0.3 993,641 + rsuA OKGIIK_04535 0.80 +0.2 993,641 + rsuA OKGIIK_04535 0.80 +0.3 993,641 + rsuA OKGIIK_04535 0.80 -1.2 993,641 + rsuA OKGIIK_04535 0.80 -1.9 993,641 + rsuA OKGIIK_04535 0.80 +0.2 993,641 + rsuA OKGIIK_04535 0.80 +0.5 993,641 + rsuA OKGIIK_04535 0.80 -0.2 993,641 + rsuA OKGIIK_04535 0.80 +0.6 993,641 + rsuA OKGIIK_04535 0.80 -1.7 993,641 + rsuA OKGIIK_04535 0.80 -0.0 993,641 + rsuA OKGIIK_04535 0.80 +0.9 993,641 + rsuA OKGIIK_04535 0.80 -0.6 993,641 + rsuA OKGIIK_04535 0.80 -0.2 993,642 - rsuA OKGIIK_04535 0.80 +0.6 993,642 - rsuA OKGIIK_04535 0.80 +1.1 993,642 - rsuA OKGIIK_04535 0.80 +0.4 993,642 - rsuA OKGIIK_04535 0.80 -1.0 993,642 - rsuA OKGIIK_04535 0.80 -0.9 993,642 - rsuA OKGIIK_04535 0.80 -0.6 993,642 - rsuA OKGIIK_04535 0.80 -0.3 993,642 - rsuA OKGIIK_04535 0.80 -0.4 993,642 - rsuA OKGIIK_04535 0.80 -0.6 993,642 - rsuA OKGIIK_04535 0.80 +0.6 993,642 - rsuA OKGIIK_04535 0.80 +0.9 993,642 - rsuA OKGIIK_04535 0.80 -0.9 993,642 - rsuA OKGIIK_04535 0.80 +0.9 993,642 - rsuA OKGIIK_04535 0.80 -0.3 993,642 - rsuA OKGIIK_04535 0.80 -1.5 993,642 - rsuA OKGIIK_04535 0.80 -1.5 993,642 - rsuA OKGIIK_04535 0.80 +0.4 993,642 - rsuA OKGIIK_04535 0.80 +0.1 993,642 - rsuA OKGIIK_04535 0.80 -1.5 993,642 - rsuA OKGIIK_04535 0.80 +0.1 993,642 - rsuA OKGIIK_04535 0.80 -0.9 993,642 - rsuA OKGIIK_04535 0.80 -1.7 993,642 - rsuA OKGIIK_04535 0.80 -0.1 993,642 - rsuA OKGIIK_04535 0.80 -0.1 993,642 - rsuA OKGIIK_04535 0.80 +1.1 993,642 - rsuA OKGIIK_04535 0.80 -0.7 993,746 + +1.4 993,746 + -0.8 993,746 + -0.3 993,746 + -1.4 993,746 + +0.8 993,746 + +0.8 993,746 + -1.5 993,746 + -0.4 993,746 + -0.7 993,747 - -0.9 994,017 - ssuD OKGIIK_04540 0.20 +0.1 994,017 - ssuD OKGIIK_04540 0.20 +1.3 994,017 - ssuD OKGIIK_04540 0.20 -0.3 994,110 + ssuD OKGIIK_04540 0.29 -1.5 994,110 + ssuD OKGIIK_04540 0.29 -0.1 994,185 + ssuD OKGIIK_04540 0.37 -1.9 994,186 - ssuD OKGIIK_04540 0.37 -2.0 994,236 + ssuD OKGIIK_04540 0.42 +1.4 994,236 + ssuD OKGIIK_04540 0.42 -0.9 994,236 + ssuD OKGIIK_04540 0.42 -0.3 994,237 - ssuD OKGIIK_04540 0.42 -1.6 994,237 - ssuD OKGIIK_04540 0.42 +0.5 994,416 + ssuD OKGIIK_04540 0.60 -1.7 994,416 + ssuD OKGIIK_04540 0.60 +0.5 994,417 - ssuD OKGIIK_04540 0.60 +0.2 994,458 + ssuD OKGIIK_04540 0.64 -0.3 994,459 - ssuD OKGIIK_04540 0.64 -0.9 994,459 - ssuD OKGIIK_04540 0.64 -0.1 994,602 + ssuD OKGIIK_04540 0.78 +0.7 994,602 + ssuD OKGIIK_04540 0.78 +0.0 994,603 - ssuD OKGIIK_04540 0.78 +0.5 994,603 - ssuD OKGIIK_04540 0.78 +0.0 994,861 + -0.2 994,861 + -1.3 994,862 - -1.2 994,862 - +0.1 994,862 - +0.4 994,862 - -0.7 994,871 + -0.5 994,871 + -2.3 994,872 - -0.5 994,872 - +0.4 994,872 - -0.9 994,995 + rIO1 OKGIIK_04545 0.11 -0.0 994,995 + rIO1 OKGIIK_04545 0.11 +0.8 994,995 + rIO1 OKGIIK_04545 0.11 +0.4 994,996 - rIO1 OKGIIK_04545 0.11 +0.3 994,996 - rIO1 OKGIIK_04545 0.11 -0.2 995,037 + rIO1 OKGIIK_04545 0.16 -1.1 995,037 + rIO1 OKGIIK_04545 0.16 -0.1 995,037 + rIO1 OKGIIK_04545 0.16 +1.2 995,037 + rIO1 OKGIIK_04545 0.16 -0.6 995,037 + rIO1 OKGIIK_04545 0.16 +0.8 995,037 + rIO1 OKGIIK_04545 0.16 -1.0 995,037 + rIO1 OKGIIK_04545 0.16 -0.6 995,038 - rIO1 OKGIIK_04545 0.16 +0.2 995,038 - rIO1 OKGIIK_04545 0.16 +0.4 995,038 - rIO1 OKGIIK_04545 0.16 -0.2 995,038 - rIO1 OKGIIK_04545 0.16 +1.3 995,038 - rIO1 OKGIIK_04545 0.16 +0.1 995,038 - rIO1 OKGIIK_04545 0.16 -1.2 995,038 - rIO1 OKGIIK_04545 0.16 -0.2 995,079 + rIO1 OKGIIK_04545 0.21 -0.8 995,079 + rIO1 OKGIIK_04545 0.21 +1.0 995,080 - rIO1 OKGIIK_04545 0.21 -0.1 995,196 + rIO1 OKGIIK_04545 0.34 +0.9 995,196 + rIO1 OKGIIK_04545 0.34 +0.3 995,196 + rIO1 OKGIIK_04545 0.34 +0.1 995,196 + rIO1 OKGIIK_04545 0.34 +0.8 995,196 + rIO1 OKGIIK_04545 0.34 -0.1 995,196 + rIO1 OKGIIK_04545 0.34 +0.4 995,196 + rIO1 OKGIIK_04545 0.34 +1.2 995,197 - rIO1 OKGIIK_04545 0.34 -1.0 995,197 - rIO1 OKGIIK_04545 0.34 +1.6 995,197 - rIO1 OKGIIK_04545 0.34 +0.5 995,197 - rIO1 OKGIIK_04545 0.34 -0.2 995,197 - rIO1 OKGIIK_04545 0.34 -1.7 995,235 + rIO1 OKGIIK_04545 0.39 +1.0 995,236 - rIO1 OKGIIK_04545 0.39 -0.2 995,236 - rIO1 OKGIIK_04545 0.39 -0.5 995,236 - rIO1 OKGIIK_04545 0.39 -0.3 995,241 + rIO1 OKGIIK_04545 0.40 +0.9 995,241 + rIO1 OKGIIK_04545 0.40 +0.4 995,241 + rIO1 OKGIIK_04545 0.40 +0.2 995,242 - rIO1 OKGIIK_04545 0.40 +1.3 995,343 + rIO1 OKGIIK_04545 0.51 +0.6 995,344 - rIO1 OKGIIK_04545 0.52 +0.7 995,415 + rIO1 OKGIIK_04545 0.60 +0.1 995,415 + rIO1 OKGIIK_04545 0.60 -0.1 995,415 + rIO1 OKGIIK_04545 0.60 -1.5 995,415 + rIO1 OKGIIK_04545 0.60 -0.5 995,415 + rIO1 OKGIIK_04545 0.60 +1.1 995,416 - rIO1 OKGIIK_04545 0.60 +1.3 995,416 - rIO1 OKGIIK_04545 0.60 -1.2 995,416 - rIO1 OKGIIK_04545 0.60 +0.2 995,416 - rIO1 OKGIIK_04545 0.60 -0.9 995,525 + rIO1 OKGIIK_04545 0.73 -0.9 995,525 + rIO1 OKGIIK_04545 0.73 -0.2 995,525 + rIO1 OKGIIK_04545 0.73 +0.1 995,525 + rIO1 OKGIIK_04545 0.73 +0.1 995,525 + rIO1 OKGIIK_04545 0.73 +1.5 995,525 + rIO1 OKGIIK_04545 0.73 +0.2 995,525 + rIO1 OKGIIK_04545 0.73 -1.5 995,525 + rIO1 OKGIIK_04545 0.73 +0.4 995,526 - rIO1 OKGIIK_04545 0.73 -0.9 995,526 - rIO1 OKGIIK_04545 0.73 -0.5 995,526 - rIO1 OKGIIK_04545 0.73 +2.1 995,526 - rIO1 OKGIIK_04545 0.73 +0.6 995,526 - rIO1 OKGIIK_04545 0.73 +1.1 995,526 - rIO1 OKGIIK_04545 0.73 -0.9 995,526 - rIO1 OKGIIK_04545 0.73 +0.4 995,526 - rIO1 OKGIIK_04545 0.73 -0.3 995,526 - rIO1 OKGIIK_04545 0.73 +1.6 995,526 - rIO1 OKGIIK_04545 0.73 -0.1 995,526 - rIO1 OKGIIK_04545 0.73 +0.1 995,526 - rIO1 OKGIIK_04545 0.73 -0.1 995,526 - rIO1 OKGIIK_04545 0.73 +1.1 995,531 + rIO1 OKGIIK_04545 0.73 -1.2 995,531 + rIO1 OKGIIK_04545 0.73 +0.5 995,531 + rIO1 OKGIIK_04545 0.73 +0.4 995,531 + rIO1 OKGIIK_04545 0.73 +1.0 995,532 - rIO1 OKGIIK_04545 0.73 +0.5 995,532 - rIO1 OKGIIK_04545 0.73 -1.5 995,574 + rIO1 OKGIIK_04545 0.78 -0.7 995,598 + rIO1 OKGIIK_04545 0.81 +0.5 995,598 + rIO1 OKGIIK_04545 0.81 +0.6 995,598 + rIO1 OKGIIK_04545 0.81 -0.9 995,598 + rIO1 OKGIIK_04545 0.81 -1.9 995,598 + rIO1 OKGIIK_04545 0.81 -0.8 995,599 - rIO1 OKGIIK_04545 0.81 +0.1 995,599 - rIO1 OKGIIK_04545 0.81 +0.1 995,599 - rIO1 OKGIIK_04545 0.81 -0.2 995,599 - rIO1 OKGIIK_04545 0.81 -1.5
Or see this region's nucleotide sequence