Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_03795 and grxC are separated by 127 nucleotides grxC and yurZ overlap by 4 nucleotides yurZ and leuB are separated by 191 nucleotides leuB and OKGIIK_03815 are separated by 276 nucleotides OKGIIK_03815 and OKGIIK_03820 are separated by 36 nucleotides
OKGIIK_03795: OKGIIK_03795 - Putative beta-barrel assembly-enhancing protease, at 837,829 to 839,532
_03795
OKGIIK_03800: grxC - glutaredoxin 3, at 839,660 to 839,923
grxC
OKGIIK_03805: yurZ - carboxymuconolactone decarboxylase family protein, at 839,920 to 840,306
yurZ
OKGIIK_03810: leuB - isocitrate dehydrogenase, at 840,498 to 841,511
leuB
OKGIIK_03815: OKGIIK_03815 - Carboxymuconolactone decarboxylase, at 841,788 to 842,249
_03815
OKGIIK_03820: OKGIIK_03820 - RNA polymerase sigma-70 factor, ECF subfamily, at 842,286 to 843,185
_03820
Position (kb) 840
841
842 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.532 kb on + strand at 839.533 kb on - strand at 839.533 kb on - strand at 839.533 kb on - strand at 839.533 kb on - strand at 839.533 kb on - strand at 839.533 kb on - strand at 839.533 kb on - strand at 839.533 kb on - strand at 839.533 kb on - strand at 839.533 kb on - strand at 839.533 kb on - strand at 839.533 kb on - strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.547 kb on + strand at 839.548 kb on - strand at 839.548 kb on - strand at 839.548 kb on - strand at 839.548 kb on - strand at 839.548 kb on - strand at 839.548 kb on - strand at 839.548 kb on - strand at 839.548 kb on - strand at 839.622 kb on + strand at 839.623 kb on - strand at 839.623 kb on - strand at 839.678 kb on + strand at 839.678 kb on + strand at 839.678 kb on + strand at 839.678 kb on + strand at 839.678 kb on + strand at 839.679 kb on - strand at 839.679 kb on - strand at 839.679 kb on - strand at 839.679 kb on - strand at 839.679 kb on - strand at 839.679 kb on - strand at 839.679 kb on - strand at 839.679 kb on - strand at 839.691 kb on + strand, within grxC at 839.691 kb on + strand, within grxC at 839.692 kb on - strand, within grxC at 839.692 kb on - strand, within grxC at 839.699 kb on + strand, within grxC at 839.699 kb on + strand, within grxC at 839.699 kb on + strand, within grxC at 839.832 kb on - strand, within grxC at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.031 kb on + strand, within yurZ at 840.032 kb on - strand, within yurZ at 840.032 kb on - strand, within yurZ at 840.032 kb on - strand, within yurZ at 840.032 kb on - strand, within yurZ at 840.032 kb on - strand, within yurZ at 840.032 kb on - strand, within yurZ at 840.032 kb on - strand, within yurZ at 840.032 kb on - strand, within yurZ at 840.032 kb on - strand, within yurZ at 840.032 kb on - strand, within yurZ at 840.032 kb on - strand, within yurZ at 840.032 kb on - strand, within yurZ at 840.118 kb on + strand, within yurZ at 840.118 kb on + strand, within yurZ at 840.118 kb on + strand, within yurZ at 840.118 kb on + strand, within yurZ at 840.118 kb on + strand, within yurZ at 840.118 kb on + strand, within yurZ at 840.119 kb on - strand, within yurZ at 840.119 kb on - strand, within yurZ at 840.119 kb on - strand, within yurZ at 840.119 kb on - strand, within yurZ at 840.119 kb on - strand, within yurZ at 840.119 kb on - strand, within yurZ at 840.119 kb on - strand, within yurZ at 840.119 kb on - strand, within yurZ at 840.119 kb on - strand, within yurZ at 840.304 kb on + strand at 840.305 kb on - strand at 840.305 kb on - strand at 840.305 kb on - strand at 840.305 kb on - strand at 840.305 kb on - strand at 840.364 kb on + strand at 840.364 kb on + strand at 840.419 kb on + strand at 841.512 kb on + strand at 841.512 kb on + strand at 841.512 kb on + strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.629 kb on + strand at 841.629 kb on + strand at 841.629 kb on + strand at 841.629 kb on + strand at 841.760 kb on + strand at 841.760 kb on + strand at 841.760 kb on + strand at 841.760 kb on + strand at 841.760 kb on + strand at 841.761 kb on - strand at 841.761 kb on - strand at 841.761 kb on - strand at 841.777 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.927 kb on + strand, within OKGIIK_03815 at 841.928 kb on - strand, within OKGIIK_03815 at 842.011 kb on + strand, within OKGIIK_03815 at 842.011 kb on + strand, within OKGIIK_03815 at 842.011 kb on + strand, within OKGIIK_03815 at 842.011 kb on + strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.104 kb on + strand, within OKGIIK_03815 at 842.104 kb on + strand, within OKGIIK_03815 at 842.104 kb on + strand, within OKGIIK_03815 at 842.104 kb on + strand, within OKGIIK_03815 at 842.104 kb on + strand, within OKGIIK_03815 at 842.105 kb on - strand, within OKGIIK_03815 at 842.221 kb on + strand at 842.221 kb on + strand at 842.221 kb on + strand at 842.221 kb on + strand at 842.222 kb on - strand at 842.222 kb on - strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.470 kb on + strand, within OKGIIK_03820 at 842.470 kb on + strand, within OKGIIK_03820 at 842.482 kb on + strand, within OKGIIK_03820 at 842.482 kb on + strand, within OKGIIK_03820 at 842.482 kb on + strand, within OKGIIK_03820 at 842.482 kb on + strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.491 kb on - strand, within OKGIIK_03820 at 842.492 kb on + strand, within OKGIIK_03820 at 842.492 kb on + strand, within OKGIIK_03820 at 842.492 kb on + strand, within OKGIIK_03820 at 842.492 kb on + strand, within OKGIIK_03820 at 842.492 kb on + strand, within OKGIIK_03820 at 842.492 kb on + strand, within OKGIIK_03820 at 842.493 kb on - strand, within OKGIIK_03820 at 842.493 kb on - strand, within OKGIIK_03820
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 839,532 + -0.5 839,532 + +0.1 839,532 + -1.5 839,532 + -0.1 839,532 + +0.1 839,532 + -0.3 839,532 + -0.7 839,532 + +0.0 839,532 + +0.7 839,532 + -0.7 839,532 + -0.0 839,532 + -1.9 839,532 + -1.0 839,532 + -0.5 839,532 + +1.0 839,532 + -0.0 839,532 + -0.5 839,532 + -1.4 839,532 + +0.6 839,533 - +0.8 839,533 - +0.0 839,533 - +0.5 839,533 - +0.0 839,533 - +0.2 839,533 - -0.7 839,533 - +0.9 839,533 - -0.7 839,533 - -0.7 839,533 - -0.7 839,533 - +1.5 839,533 - -0.3 839,547 + +0.1 839,547 + -1.9 839,547 + -0.3 839,547 + +0.0 839,547 + -0.3 839,547 + -1.2 839,547 + +0.1 839,547 + -0.7 839,547 + +0.8 839,547 + -1.9 839,547 + -0.3 839,547 + -1.4 839,547 + +1.5 839,547 + +1.1 839,547 + -0.3 839,547 + +1.6 839,548 - -0.9 839,548 - -0.5 839,548 - +1.1 839,548 - +0.6 839,548 - +1.3 839,548 - +0.6 839,548 - +1.1 839,548 - +0.8 839,622 + +0.1 839,623 - -0.7 839,623 - -0.4 839,678 + -0.4 839,678 + -0.5 839,678 + -1.5 839,678 + -1.0 839,678 + -0.3 839,679 - +0.4 839,679 - -0.4 839,679 - +0.9 839,679 - -0.2 839,679 - -0.1 839,679 - +0.7 839,679 - -1.2 839,679 - +0.4 839,691 + grxC OKGIIK_03800 0.12 +0.1 839,691 + grxC OKGIIK_03800 0.12 -0.4 839,692 - grxC OKGIIK_03800 0.12 -0.4 839,692 - grxC OKGIIK_03800 0.12 -0.9 839,699 + grxC OKGIIK_03800 0.15 +0.1 839,699 + grxC OKGIIK_03800 0.15 +0.3 839,699 + grxC OKGIIK_03800 0.15 -1.0 839,832 - grxC OKGIIK_03800 0.65 -0.4 840,031 + yurZ OKGIIK_03805 0.29 -0.9 840,031 + yurZ OKGIIK_03805 0.29 +0.3 840,031 + yurZ OKGIIK_03805 0.29 +0.5 840,031 + yurZ OKGIIK_03805 0.29 +0.1 840,031 + yurZ OKGIIK_03805 0.29 -0.3 840,031 + yurZ OKGIIK_03805 0.29 +0.3 840,031 + yurZ OKGIIK_03805 0.29 +0.1 840,031 + yurZ OKGIIK_03805 0.29 +0.0 840,031 + yurZ OKGIIK_03805 0.29 +0.2 840,031 + yurZ OKGIIK_03805 0.29 +1.1 840,031 + yurZ OKGIIK_03805 0.29 +0.4 840,031 + yurZ OKGIIK_03805 0.29 +0.7 840,031 + yurZ OKGIIK_03805 0.29 +0.1 840,031 + yurZ OKGIIK_03805 0.29 -0.9 840,031 + yurZ OKGIIK_03805 0.29 +0.1 840,031 + yurZ OKGIIK_03805 0.29 +1.3 840,031 + yurZ OKGIIK_03805 0.29 -1.0 840,032 - yurZ OKGIIK_03805 0.29 +1.3 840,032 - yurZ OKGIIK_03805 0.29 -0.4 840,032 - yurZ OKGIIK_03805 0.29 -1.9 840,032 - yurZ OKGIIK_03805 0.29 -1.7 840,032 - yurZ OKGIIK_03805 0.29 +1.0 840,032 - yurZ OKGIIK_03805 0.29 -0.4 840,032 - yurZ OKGIIK_03805 0.29 -0.6 840,032 - yurZ OKGIIK_03805 0.29 -0.9 840,032 - yurZ OKGIIK_03805 0.29 +1.4 840,032 - yurZ OKGIIK_03805 0.29 +0.7 840,032 - yurZ OKGIIK_03805 0.29 +0.6 840,032 - yurZ OKGIIK_03805 0.29 -0.7 840,118 + yurZ OKGIIK_03805 0.51 -0.6 840,118 + yurZ OKGIIK_03805 0.51 +1.3 840,118 + yurZ OKGIIK_03805 0.51 -0.6 840,118 + yurZ OKGIIK_03805 0.51 +0.1 840,118 + yurZ OKGIIK_03805 0.51 +1.1 840,118 + yurZ OKGIIK_03805 0.51 +0.1 840,119 - yurZ OKGIIK_03805 0.51 +1.4 840,119 - yurZ OKGIIK_03805 0.51 -0.8 840,119 - yurZ OKGIIK_03805 0.51 +0.7 840,119 - yurZ OKGIIK_03805 0.51 +0.8 840,119 - yurZ OKGIIK_03805 0.51 -3.0 840,119 - yurZ OKGIIK_03805 0.51 -0.1 840,119 - yurZ OKGIIK_03805 0.51 -0.1 840,119 - yurZ OKGIIK_03805 0.51 -0.1 840,119 - yurZ OKGIIK_03805 0.51 -0.0 840,304 + +0.2 840,305 - +1.7 840,305 - -1.2 840,305 - -0.9 840,305 - -0.1 840,305 - -0.2 840,364 + -1.2 840,364 + -0.4 840,419 + -1.1 841,512 + +0.0 841,512 + +0.7 841,512 + -0.4 841,513 - -1.3 841,513 - +0.1 841,513 - -0.4 841,513 - -0.3 841,513 - -0.4 841,513 - -1.4 841,513 - +0.1 841,513 - +0.8 841,513 - -0.1 841,513 - -0.1 841,513 - -0.4 841,513 - +0.6 841,629 + +0.3 841,629 + -0.6 841,629 + +0.6 841,629 + -1.4 841,760 + -0.9 841,760 + +0.3 841,760 + +0.1 841,760 + -0.9 841,760 + -2.0 841,761 - +0.3 841,761 - -0.1 841,761 - -0.1 841,777 + -1.3 841,810 + +0.8 841,810 + +0.3 841,810 + -0.5 841,810 + -1.4 841,810 + +0.1 841,810 + +0.2 841,810 + -1.2 841,810 + -0.3 841,810 + -0.6 841,811 - -2.2 841,811 - -0.1 841,811 - -0.4 841,811 - -2.1 841,811 - -1.2 841,811 - -1.5 841,811 - +0.1 841,811 - -0.4 841,811 - +0.8 841,927 + OKGIIK_03815 0.30 -0.6 841,928 - OKGIIK_03815 0.30 -0.5 842,011 + OKGIIK_03815 0.48 -1.3 842,011 + OKGIIK_03815 0.48 -0.3 842,011 + OKGIIK_03815 0.48 -0.7 842,011 + OKGIIK_03815 0.48 +0.1 842,012 - OKGIIK_03815 0.48 -0.2 842,012 - OKGIIK_03815 0.48 -2.7 842,012 - OKGIIK_03815 0.48 -1.9 842,012 - OKGIIK_03815 0.48 +0.3 842,012 - OKGIIK_03815 0.48 +0.5 842,012 - OKGIIK_03815 0.48 +0.3 842,012 - OKGIIK_03815 0.48 -0.4 842,104 + OKGIIK_03815 0.68 -0.3 842,104 + OKGIIK_03815 0.68 +1.1 842,104 + OKGIIK_03815 0.68 -0.7 842,104 + OKGIIK_03815 0.68 -1.2 842,104 + OKGIIK_03815 0.68 +0.7 842,105 - OKGIIK_03815 0.69 -1.7 842,221 + +0.3 842,221 + +2.1 842,221 + -0.1 842,221 + -0.0 842,222 - -0.2 842,222 - -1.9 842,249 + -0.8 842,249 + -0.5 842,249 + -0.3 842,249 + -0.4 842,249 + +1.1 842,249 + -0.3 842,249 + +1.0 842,249 + +0.3 842,249 + -1.4 842,249 + -1.0 842,249 + +0.6 842,249 + -0.2 842,249 + +0.0 842,249 + +0.1 842,249 + -1.0 842,249 + -1.4 842,249 + +1.1 842,249 + +1.1 842,250 - +1.0 842,250 - -0.1 842,250 - +0.1 842,250 - +0.4 842,250 - -0.4 842,250 - -1.2 842,250 - -0.9 842,250 - -0.9 842,250 - -1.7 842,250 - -1.4 842,470 + OKGIIK_03820 0.20 -1.3 842,470 + OKGIIK_03820 0.20 -1.7 842,482 + OKGIIK_03820 0.22 -0.9 842,482 + OKGIIK_03820 0.22 -0.7 842,482 + OKGIIK_03820 0.22 +0.1 842,482 + OKGIIK_03820 0.22 +1.7 842,483 - OKGIIK_03820 0.22 +0.1 842,483 - OKGIIK_03820 0.22 +0.6 842,483 - OKGIIK_03820 0.22 +0.0 842,483 - OKGIIK_03820 0.22 -2.0 842,483 - OKGIIK_03820 0.22 -2.0 842,483 - OKGIIK_03820 0.22 -1.2 842,483 - OKGIIK_03820 0.22 -4.9 842,491 - OKGIIK_03820 0.23 +0.3 842,492 + OKGIIK_03820 0.23 -0.2 842,492 + OKGIIK_03820 0.23 -1.0 842,492 + OKGIIK_03820 0.23 +0.4 842,492 + OKGIIK_03820 0.23 -0.5 842,492 + OKGIIK_03820 0.23 +0.4 842,492 + OKGIIK_03820 0.23 -0.4 842,493 - OKGIIK_03820 0.23 +0.3 842,493 - OKGIIK_03820 0.23 +1.4
Or see this region's nucleotide sequence