Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_03035 and OKGIIK_03040 are separated by 115 nucleotides OKGIIK_03040 and purE are separated by 133 nucleotides purE and purK overlap by 4 nucleotides purK and sodA are separated by 50 nucleotides sodA and grxD are separated by 115 nucleotides
OKGIIK_03035: OKGIIK_03035 - hypothetical protein, at 688,631 to 691,780
_03035
OKGIIK_03040: OKGIIK_03040 - hypothetical protein, at 691,896 to 692,165
_03040
OKGIIK_03045: purE - Phosphoribosylcarboxyaminoimidazole (NCAIR) mutase, at 692,299 to 692,736
purE
OKGIIK_03050: purK - 5-(carboxyamino)imidazole ribonucleotide synthase, at 692,733 to 693,923
purK
OKGIIK_03055: sodA - superoxide dismutase [Fe], at 693,974 to 694,552
sodA
OKGIIK_03060: grxD - Grx4 family monothiol glutaredoxin, at 694,668 to 694,991
grxD
Position (kb) 692
693
694 Strain fitness (log2 ratio) -2
-1
0
1
2 at 691.837 kb on + strand at 691.837 kb on + strand at 691.838 kb on - strand at 691.838 kb on - strand at 691.846 kb on + strand at 691.847 kb on - strand at 691.847 kb on - strand at 691.860 kb on + strand at 691.897 kb on + strand at 691.897 kb on + strand at 691.898 kb on - strand at 691.898 kb on - strand at 691.978 kb on + strand, within OKGIIK_03040 at 691.978 kb on + strand, within OKGIIK_03040 at 691.978 kb on + strand, within OKGIIK_03040 at 691.978 kb on + strand, within OKGIIK_03040 at 691.978 kb on + strand, within OKGIIK_03040 at 691.979 kb on - strand, within OKGIIK_03040 at 691.979 kb on - strand, within OKGIIK_03040 at 691.979 kb on - strand, within OKGIIK_03040 at 691.979 kb on - strand, within OKGIIK_03040 at 692.204 kb on + strand at 692.204 kb on + strand at 692.205 kb on - strand at 692.205 kb on - strand at 692.755 kb on + strand at 692.755 kb on + strand at 693.898 kb on - strand at 693.898 kb on - strand at 693.898 kb on - strand at 693.898 kb on - strand at 693.898 kb on - strand at 693.898 kb on - strand at 693.898 kb on - strand at 693.898 kb on - strand at 693.898 kb on - strand at 693.898 kb on - strand at 693.953 kb on + strand at 694.624 kb on + strand at 694.624 kb on + strand at 694.624 kb on + strand at 694.624 kb on + strand at 694.624 kb on + strand at 694.624 kb on + strand at 694.624 kb on + strand at 694.624 kb on + strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.625 kb on - strand at 694.629 kb on + strand at 694.648 kb on + strand at 694.648 kb on + strand at 694.648 kb on + strand at 694.648 kb on + strand at 694.649 kb on - strand at 694.649 kb on - strand at 694.649 kb on - strand at 694.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 691,837 + -1.3 691,837 + -0.8 691,838 - -0.2 691,838 - -1.1 691,846 + -0.6 691,847 - +0.9 691,847 - -0.9 691,860 + -0.5 691,897 + -1.7 691,897 + -0.3 691,898 - -0.8 691,898 - -1.5 691,978 + OKGIIK_03040 0.30 +0.9 691,978 + OKGIIK_03040 0.30 -0.2 691,978 + OKGIIK_03040 0.30 +0.7 691,978 + OKGIIK_03040 0.30 +0.7 691,978 + OKGIIK_03040 0.30 +0.2 691,979 - OKGIIK_03040 0.31 +0.7 691,979 - OKGIIK_03040 0.31 -0.5 691,979 - OKGIIK_03040 0.31 -0.9 691,979 - OKGIIK_03040 0.31 +0.2 692,204 + -0.3 692,204 + -0.5 692,205 - -0.6 692,205 - -1.5 692,755 + -1.4 692,755 + +0.1 693,898 - -0.3 693,898 - +0.7 693,898 - -1.3 693,898 - -0.5 693,898 - -0.5 693,898 - -0.8 693,898 - +0.1 693,898 - +1.0 693,898 - -0.2 693,898 - +0.9 693,953 + +1.9 694,624 + -0.3 694,624 + +1.1 694,624 + -1.7 694,624 + +0.3 694,624 + -0.5 694,624 + +0.3 694,624 + -1.1 694,624 + -1.1 694,625 - -1.3 694,625 - +0.1 694,625 - -0.9 694,625 - -0.2 694,625 - -1.3 694,625 - -0.3 694,625 - -1.0 694,625 - -2.1 694,625 - -0.2 694,625 - -0.1 694,625 - +0.4 694,625 - -0.5 694,625 - +0.5 694,625 - +0.3 694,625 - -0.0 694,625 - +0.9 694,625 - -1.7 694,625 - +0.4 694,625 - -0.2 694,625 - -0.1 694,625 - -2.2 694,625 - -1.7 694,625 - -0.1 694,625 - +0.1 694,625 - -0.1 694,625 - -0.0 694,629 + -1.6 694,648 + -0.8 694,648 + -1.4 694,648 + -0.4 694,648 + +0.5 694,649 - +1.6 694,649 - +0.4 694,649 - +0.7 694,673 + -0.1
Or see this region's nucleotide sequence