Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lptB and lptA are separated by 3 nucleotides lptA and lptC overlap by 38 nucleotides lptC and OKGIIK_02285 overlap by 4 nucleotides OKGIIK_02285 and gutQ are separated by 66 nucleotides
OKGIIK_02270: lptB - LPS export ABC transporter ATP-binding protein, at 531,691 to 532,410
lptB
OKGIIK_02275: lptA - lipopolysaccharide transport periplasmic protein LptA, at 532,414 to 532,998
lptA
OKGIIK_02280: lptC - LPS export ABC transporter periplasmic protein LptC, at 532,961 to 533,557
lptC
OKGIIK_02285: OKGIIK_02285 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC, at 533,554 to 534,102
_02285
OKGIIK_02290: gutQ - D-arabinose 5-phosphate isomerase, at 534,169 to 535,188
gutQ
Position (kb) 532
533
534 Strain fitness (log2 ratio) -2
-1
0
1 at 534.114 kb on + strand at 534.114 kb on + strand at 534.114 kb on + strand at 534.114 kb on + strand at 534.114 kb on + strand at 534.115 kb on - strand at 534.115 kb on - strand at 534.115 kb on - strand at 534.115 kb on - strand at 534.115 kb on - strand at 534.115 kb on - strand at 534.115 kb on - strand at 534.115 kb on - strand at 534.115 kb on - strand at 534.115 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 534,114 + -0.5 534,114 + -1.4 534,114 + +0.8 534,114 + -0.8 534,114 + -0.4 534,115 - -0.1 534,115 - +1.1 534,115 - -2.1 534,115 - +0.2 534,115 - +0.6 534,115 - +0.4 534,115 - -0.4 534,115 - -0.1 534,115 - -0.2 534,115 - -0.2
Or see this region's nucleotide sequence