Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and ybgC overlap by 8 nucleotides ybgC and tolQ are separated by 39 nucleotides tolQ and tolR are separated by 13 nucleotides
OKGIIK_01850: ruvB - Holliday junction branch migration DNA helicase RuvB, at 429,612 to 430,661
ruvB
OKGIIK_01855: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 430,654 to 431,073
ybgC
OKGIIK_01860: tolQ - protein TolQ, at 431,113 to 431,799
tolQ
OKGIIK_01865: tolR - protein TolR, at 431,813 to 432,277
tolR
Position (kb) 430
431
432 Strain fitness (log2 ratio) -2
-1
0
1 at 430.159 kb on - strand, within ruvB at 430.694 kb on + strand at 430.694 kb on + strand at 430.694 kb on + strand at 430.694 kb on + strand at 430.694 kb on + strand at 430.694 kb on + strand at 430.694 kb on + strand at 430.695 kb on - strand at 430.695 kb on - strand at 430.695 kb on - strand at 430.702 kb on + strand, within ybgC at 430.702 kb on + strand, within ybgC at 430.702 kb on + strand, within ybgC at 430.702 kb on + strand, within ybgC at 430.702 kb on + strand, within ybgC at 430.702 kb on + strand, within ybgC at 430.703 kb on - strand, within ybgC at 430.735 kb on + strand, within ybgC at 430.735 kb on + strand, within ybgC at 430.735 kb on + strand, within ybgC at 430.735 kb on + strand, within ybgC at 430.735 kb on + strand, within ybgC at 430.735 kb on + strand, within ybgC at 430.736 kb on - strand, within ybgC at 430.736 kb on - strand, within ybgC at 430.736 kb on - strand, within ybgC at 430.736 kb on - strand, within ybgC at 430.736 kb on - strand, within ybgC at 430.748 kb on - strand, within ybgC at 430.935 kb on + strand, within ybgC at 430.935 kb on + strand, within ybgC at 430.935 kb on + strand, within ybgC at 430.936 kb on - strand, within ybgC at 430.936 kb on - strand, within ybgC
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 430,159 - ruvB OKGIIK_01850 0.52 -0.9 430,694 + +0.9 430,694 + +0.4 430,694 + -0.2 430,694 + +0.5 430,694 + +0.1 430,694 + -0.9 430,694 + -0.4 430,695 - +0.3 430,695 - +1.1 430,695 - -1.9 430,702 + ybgC OKGIIK_01855 0.11 +0.6 430,702 + ybgC OKGIIK_01855 0.11 -0.8 430,702 + ybgC OKGIIK_01855 0.11 +0.7 430,702 + ybgC OKGIIK_01855 0.11 -2.5 430,702 + ybgC OKGIIK_01855 0.11 -1.3 430,702 + ybgC OKGIIK_01855 0.11 +0.1 430,703 - ybgC OKGIIK_01855 0.12 -1.3 430,735 + ybgC OKGIIK_01855 0.19 -0.5 430,735 + ybgC OKGIIK_01855 0.19 -1.9 430,735 + ybgC OKGIIK_01855 0.19 -0.7 430,735 + ybgC OKGIIK_01855 0.19 -1.6 430,735 + ybgC OKGIIK_01855 0.19 -0.6 430,735 + ybgC OKGIIK_01855 0.19 -0.3 430,736 - ybgC OKGIIK_01855 0.20 -1.5 430,736 - ybgC OKGIIK_01855 0.20 -1.5 430,736 - ybgC OKGIIK_01855 0.20 +0.5 430,736 - ybgC OKGIIK_01855 0.20 +1.6 430,736 - ybgC OKGIIK_01855 0.20 +1.4 430,748 - ybgC OKGIIK_01855 0.22 -0.7 430,935 + ybgC OKGIIK_01855 0.67 +0.1 430,935 + ybgC OKGIIK_01855 0.67 -0.3 430,935 + ybgC OKGIIK_01855 0.67 +0.7 430,936 - ybgC OKGIIK_01855 0.67 -0.2 430,936 - ybgC OKGIIK_01855 0.67 +1.5
Or see this region's nucleotide sequence