Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_01280 and fixX overlap by 4 nucleotides fixX and gatA are separated by 162 nucleotides
OKGIIK_01280: OKGIIK_01280 - Glyco-trans-2-like domain-containing protein, at 280,252 to 282,354
_01280
OKGIIK_01285: fixX - electron transfer flavoprotein-ubiquinone oxidoreductase, at 282,351 to 283,964
fixX
OKGIIK_01290: gatA - amidase, at 284,127 to 285,524
gatA
Position (kb) 282
283
284 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 281.519 kb on + strand, within OKGIIK_01280 at 281.519 kb on + strand, within OKGIIK_01280 at 281.519 kb on + strand, within OKGIIK_01280 at 281.519 kb on + strand, within OKGIIK_01280 at 281.520 kb on - strand, within OKGIIK_01280 at 281.520 kb on - strand, within OKGIIK_01280 at 281.520 kb on - strand, within OKGIIK_01280 at 281.520 kb on - strand, within OKGIIK_01280 at 281.522 kb on + strand, within OKGIIK_01280 at 281.522 kb on + strand, within OKGIIK_01280 at 281.522 kb on + strand, within OKGIIK_01280 at 281.523 kb on - strand, within OKGIIK_01280 at 281.523 kb on - strand, within OKGIIK_01280 at 281.573 kb on + strand, within OKGIIK_01280 at 281.573 kb on + strand, within OKGIIK_01280 at 281.574 kb on - strand, within OKGIIK_01280 at 281.574 kb on - strand, within OKGIIK_01280 at 281.574 kb on - strand, within OKGIIK_01280 at 281.576 kb on + strand, within OKGIIK_01280 at 281.577 kb on - strand, within OKGIIK_01280 at 281.577 kb on - strand, within OKGIIK_01280 at 281.577 kb on - strand, within OKGIIK_01280 at 281.589 kb on - strand, within OKGIIK_01280 at 281.589 kb on - strand, within OKGIIK_01280 at 281.657 kb on + strand, within OKGIIK_01280 at 281.657 kb on + strand, within OKGIIK_01280 at 281.657 kb on + strand, within OKGIIK_01280 at 281.657 kb on + strand, within OKGIIK_01280 at 281.657 kb on + strand, within OKGIIK_01280 at 281.658 kb on - strand, within OKGIIK_01280 at 281.658 kb on - strand, within OKGIIK_01280 at 281.658 kb on - strand, within OKGIIK_01280 at 281.658 kb on - strand, within OKGIIK_01280 at 281.658 kb on - strand, within OKGIIK_01280 at 281.658 kb on - strand, within OKGIIK_01280 at 281.658 kb on - strand, within OKGIIK_01280 at 281.658 kb on - strand, within OKGIIK_01280 at 281.777 kb on + strand, within OKGIIK_01280 at 282.012 kb on + strand, within OKGIIK_01280 at 282.084 kb on + strand, within OKGIIK_01280 at 282.085 kb on - strand, within OKGIIK_01280 at 282.293 kb on + strand at 282.293 kb on + strand at 282.293 kb on + strand at 282.294 kb on - strand at 282.294 kb on - strand at 282.364 kb on + strand at 282.511 kb on + strand at 282.550 kb on + strand, within fixX at 282.550 kb on + strand, within fixX at 282.640 kb on + strand, within fixX at 282.685 kb on + strand, within fixX at 282.685 kb on + strand, within fixX at 282.686 kb on - strand, within fixX at 282.686 kb on - strand, within fixX at 282.686 kb on - strand, within fixX at 283.165 kb on + strand, within fixX at 283.165 kb on + strand, within fixX at 283.166 kb on - strand, within fixX at 283.198 kb on + strand, within fixX at 283.231 kb on + strand, within fixX at 283.231 kb on + strand, within fixX at 283.231 kb on + strand, within fixX at 283.232 kb on - strand, within fixX at 283.232 kb on - strand, within fixX at 283.232 kb on - strand, within fixX at 283.232 kb on - strand, within fixX at 283.232 kb on - strand, within fixX at 283.249 kb on + strand, within fixX at 283.250 kb on - strand, within fixX at 283.250 kb on - strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.382 kb on - strand, within fixX at 283.469 kb on - strand, within fixX at 283.469 kb on - strand, within fixX at 283.546 kb on + strand, within fixX at 283.546 kb on + strand, within fixX at 283.546 kb on + strand, within fixX at 283.547 kb on - strand, within fixX at 283.547 kb on - strand, within fixX at 283.547 kb on - strand, within fixX at 283.547 kb on - strand, within fixX at 283.547 kb on - strand, within fixX at 283.547 kb on - strand, within fixX at 283.939 kb on + strand at 283.939 kb on + strand at 283.940 kb on - strand at 284.010 kb on + strand at 284.010 kb on + strand at 284.011 kb on - strand at 284.238 kb on + strand at 284.238 kb on + strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.700 kb on + strand, within gatA at 284.700 kb on + strand, within gatA at 284.700 kb on + strand, within gatA at 284.700 kb on + strand, within gatA at 284.700 kb on + strand, within gatA at 284.700 kb on + strand, within gatA at 284.701 kb on - strand, within gatA at 284.701 kb on - strand, within gatA at 284.701 kb on - strand, within gatA at 284.701 kb on - strand, within gatA at 284.701 kb on - strand, within gatA at 284.712 kb on + strand, within gatA at 284.712 kb on + strand, within gatA at 284.712 kb on + strand, within gatA at 284.712 kb on + strand, within gatA at 284.713 kb on - strand, within gatA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 281,519 + OKGIIK_01280 0.60 +0.0 281,519 + OKGIIK_01280 0.60 -0.1 281,519 + OKGIIK_01280 0.60 +0.6 281,519 + OKGIIK_01280 0.60 +0.8 281,520 - OKGIIK_01280 0.60 -2.2 281,520 - OKGIIK_01280 0.60 -1.4 281,520 - OKGIIK_01280 0.60 -1.2 281,520 - OKGIIK_01280 0.60 -0.6 281,522 + OKGIIK_01280 0.60 -1.1 281,522 + OKGIIK_01280 0.60 -1.2 281,522 + OKGIIK_01280 0.60 -1.1 281,523 - OKGIIK_01280 0.60 -1.3 281,523 - OKGIIK_01280 0.60 -1.1 281,573 + OKGIIK_01280 0.63 -1.1 281,573 + OKGIIK_01280 0.63 -1.2 281,574 - OKGIIK_01280 0.63 +1.4 281,574 - OKGIIK_01280 0.63 -0.9 281,574 - OKGIIK_01280 0.63 -1.9 281,576 + OKGIIK_01280 0.63 -0.0 281,577 - OKGIIK_01280 0.63 -1.7 281,577 - OKGIIK_01280 0.63 +2.0 281,577 - OKGIIK_01280 0.63 +0.1 281,589 - OKGIIK_01280 0.64 +1.1 281,589 - OKGIIK_01280 0.64 -0.7 281,657 + OKGIIK_01280 0.67 -1.5 281,657 + OKGIIK_01280 0.67 -0.9 281,657 + OKGIIK_01280 0.67 +0.0 281,657 + OKGIIK_01280 0.67 -0.0 281,657 + OKGIIK_01280 0.67 +0.1 281,658 - OKGIIK_01280 0.67 +0.4 281,658 - OKGIIK_01280 0.67 -0.1 281,658 - OKGIIK_01280 0.67 -0.4 281,658 - OKGIIK_01280 0.67 -0.9 281,658 - OKGIIK_01280 0.67 -0.1 281,658 - OKGIIK_01280 0.67 -0.6 281,658 - OKGIIK_01280 0.67 +0.6 281,658 - OKGIIK_01280 0.67 -0.2 281,777 + OKGIIK_01280 0.73 -2.6 282,012 + OKGIIK_01280 0.84 +1.1 282,084 + OKGIIK_01280 0.87 -1.0 282,085 - OKGIIK_01280 0.87 -1.5 282,293 + -0.1 282,293 + -2.5 282,293 + +0.3 282,294 - -1.4 282,294 - -1.9 282,364 + +0.4 282,511 + +0.3 282,550 + fixX OKGIIK_01285 0.12 -0.9 282,550 + fixX OKGIIK_01285 0.12 -0.2 282,640 + fixX OKGIIK_01285 0.18 -0.7 282,685 + fixX OKGIIK_01285 0.21 -1.7 282,685 + fixX OKGIIK_01285 0.21 -0.7 282,686 - fixX OKGIIK_01285 0.21 +0.1 282,686 - fixX OKGIIK_01285 0.21 +0.3 282,686 - fixX OKGIIK_01285 0.21 -1.2 283,165 + fixX OKGIIK_01285 0.50 +0.4 283,165 + fixX OKGIIK_01285 0.50 -0.9 283,166 - fixX OKGIIK_01285 0.50 -0.9 283,198 + fixX OKGIIK_01285 0.52 +0.7 283,231 + fixX OKGIIK_01285 0.55 -1.2 283,231 + fixX OKGIIK_01285 0.55 +0.4 283,231 + fixX OKGIIK_01285 0.55 -1.0 283,232 - fixX OKGIIK_01285 0.55 -0.2 283,232 - fixX OKGIIK_01285 0.55 -1.2 283,232 - fixX OKGIIK_01285 0.55 -1.7 283,232 - fixX OKGIIK_01285 0.55 +0.7 283,232 - fixX OKGIIK_01285 0.55 +1.0 283,249 + fixX OKGIIK_01285 0.56 -1.0 283,250 - fixX OKGIIK_01285 0.56 -1.2 283,250 - fixX OKGIIK_01285 0.56 +1.4 283,253 + fixX OKGIIK_01285 0.56 -1.5 283,253 + fixX OKGIIK_01285 0.56 -2.2 283,253 + fixX OKGIIK_01285 0.56 +1.2 283,253 + fixX OKGIIK_01285 0.56 -1.2 283,253 + fixX OKGIIK_01285 0.56 -1.2 283,253 + fixX OKGIIK_01285 0.56 -1.1 283,253 + fixX OKGIIK_01285 0.56 +0.8 283,253 + fixX OKGIIK_01285 0.56 -0.5 283,253 + fixX OKGIIK_01285 0.56 -0.8 283,253 + fixX OKGIIK_01285 0.56 -2.1 283,253 + fixX OKGIIK_01285 0.56 -0.6 283,253 + fixX OKGIIK_01285 0.56 -1.2 283,253 + fixX OKGIIK_01285 0.56 -2.1 283,253 + fixX OKGIIK_01285 0.56 +1.1 283,253 + fixX OKGIIK_01285 0.56 -1.1 283,254 - fixX OKGIIK_01285 0.56 +0.3 283,254 - fixX OKGIIK_01285 0.56 -1.4 283,254 - fixX OKGIIK_01285 0.56 -0.5 283,254 - fixX OKGIIK_01285 0.56 -0.2 283,254 - fixX OKGIIK_01285 0.56 +0.5 283,254 - fixX OKGIIK_01285 0.56 -3.4 283,254 - fixX OKGIIK_01285 0.56 -1.9 283,254 - fixX OKGIIK_01285 0.56 +0.2 283,254 - fixX OKGIIK_01285 0.56 -1.1 283,254 - fixX OKGIIK_01285 0.56 -1.2 283,254 - fixX OKGIIK_01285 0.56 -1.4 283,254 - fixX OKGIIK_01285 0.56 +1.4 283,382 - fixX OKGIIK_01285 0.64 -1.2 283,469 - fixX OKGIIK_01285 0.69 -0.8 283,469 - fixX OKGIIK_01285 0.69 -0.7 283,546 + fixX OKGIIK_01285 0.74 -0.5 283,546 + fixX OKGIIK_01285 0.74 +0.7 283,546 + fixX OKGIIK_01285 0.74 +1.0 283,547 - fixX OKGIIK_01285 0.74 -1.8 283,547 - fixX OKGIIK_01285 0.74 -0.5 283,547 - fixX OKGIIK_01285 0.74 -1.1 283,547 - fixX OKGIIK_01285 0.74 +1.8 283,547 - fixX OKGIIK_01285 0.74 +0.5 283,547 - fixX OKGIIK_01285 0.74 +0.1 283,939 + -0.1 283,939 + -1.9 283,940 - -1.9 284,010 + -0.2 284,010 + +0.0 284,011 - +1.1 284,238 + +0.2 284,238 + +1.5 284,239 - +0.9 284,239 - +0.8 284,239 - +1.0 284,239 - +0.3 284,239 - +0.7 284,239 - +1.5 284,239 - -0.0 284,239 - +0.7 284,280 + gatA OKGIIK_01290 0.11 +0.0 284,280 + gatA OKGIIK_01290 0.11 -1.5 284,280 + gatA OKGIIK_01290 0.11 -0.7 284,280 + gatA OKGIIK_01290 0.11 -2.7 284,280 + gatA OKGIIK_01290 0.11 +1.1 284,280 + gatA OKGIIK_01290 0.11 -1.1 284,280 + gatA OKGIIK_01290 0.11 -0.6 284,280 + gatA OKGIIK_01290 0.11 -1.6 284,280 + gatA OKGIIK_01290 0.11 +1.1 284,280 + gatA OKGIIK_01290 0.11 -0.3 284,280 + gatA OKGIIK_01290 0.11 -0.5 284,281 - gatA OKGIIK_01290 0.11 -0.4 284,281 - gatA OKGIIK_01290 0.11 +1.5 284,281 - gatA OKGIIK_01290 0.11 -0.8 284,281 - gatA OKGIIK_01290 0.11 +0.8 284,281 - gatA OKGIIK_01290 0.11 +0.4 284,281 - gatA OKGIIK_01290 0.11 +0.1 284,281 - gatA OKGIIK_01290 0.11 -1.5 284,281 - gatA OKGIIK_01290 0.11 -1.7 284,281 - gatA OKGIIK_01290 0.11 -0.6 284,281 - gatA OKGIIK_01290 0.11 -1.9 284,700 + gatA OKGIIK_01290 0.41 +2.1 284,700 + gatA OKGIIK_01290 0.41 -0.3 284,700 + gatA OKGIIK_01290 0.41 +0.3 284,700 + gatA OKGIIK_01290 0.41 +0.4 284,700 + gatA OKGIIK_01290 0.41 +0.3 284,700 + gatA OKGIIK_01290 0.41 +0.6 284,701 - gatA OKGIIK_01290 0.41 +1.1 284,701 - gatA OKGIIK_01290 0.41 -0.8 284,701 - gatA OKGIIK_01290 0.41 -5.2 284,701 - gatA OKGIIK_01290 0.41 +0.2 284,701 - gatA OKGIIK_01290 0.41 +0.5 284,712 + gatA OKGIIK_01290 0.42 +1.2 284,712 + gatA OKGIIK_01290 0.42 -0.3 284,712 + gatA OKGIIK_01290 0.42 +0.1 284,712 + gatA OKGIIK_01290 0.42 +0.2 284,713 - gatA OKGIIK_01290 0.42 -0.3
Or see this region's nucleotide sequence