Experiment: NF
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX19_02930 and MPMX19_02931 are separated by 156 nucleotides MPMX19_02931 and MPMX19_02932 overlap by 4 nucleotides MPMX19_02932 and MPMX19_02933 are separated by 413 nucleotides
MPMX19_02930: MPMX19_02930 - hypothetical protein, at 184,641 to 185,756
_02930
MPMX19_02931: MPMX19_02931 - Linearmycin resistance ATP-binding protein LnrL, at 185,913 to 186,701
_02931
MPMX19_02932: MPMX19_02932 - hypothetical protein, at 186,698 to 187,513
_02932
MPMX19_02933: MPMX19_02933 - Methanol dehydrogenase [cytochrome c] subunit 1, at 187,927 to 189,762
_02933
Position (kb) 186
187
188 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 185.916 kb on + strand at 185.916 kb on + strand at 185.916 kb on + strand at 185.916 kb on + strand at 185.916 kb on + strand at 185.916 kb on + strand at 185.916 kb on + strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 185.917 kb on - strand at 186.123 kb on + strand, within MPMX19_02931 at 186.123 kb on + strand, within MPMX19_02931 at 186.124 kb on - strand, within MPMX19_02931 at 186.124 kb on - strand, within MPMX19_02931 at 186.261 kb on + strand, within MPMX19_02931 at 186.262 kb on - strand, within MPMX19_02931 at 186.262 kb on - strand, within MPMX19_02931 at 186.262 kb on - strand, within MPMX19_02931 at 186.394 kb on + strand, within MPMX19_02931 at 186.713 kb on + strand at 186.713 kb on + strand at 186.713 kb on + strand at 186.713 kb on + strand at 186.713 kb on + strand at 186.713 kb on + strand at 186.713 kb on + strand at 186.714 kb on - strand at 186.714 kb on - strand at 186.714 kb on - strand at 186.714 kb on - strand at 186.719 kb on + strand at 186.719 kb on + strand at 186.720 kb on - strand at 186.720 kb on - strand at 186.870 kb on - strand, within MPMX19_02932 at 186.870 kb on - strand, within MPMX19_02932 at 186.878 kb on + strand, within MPMX19_02932 at 186.878 kb on + strand, within MPMX19_02932 at 186.878 kb on + strand, within MPMX19_02932 at 186.878 kb on + strand, within MPMX19_02932 at 186.878 kb on + strand, within MPMX19_02932 at 186.878 kb on + strand, within MPMX19_02932 at 186.878 kb on + strand, within MPMX19_02932 at 186.878 kb on + strand, within MPMX19_02932 at 186.878 kb on + strand, within MPMX19_02932 at 186.878 kb on + strand, within MPMX19_02932 at 186.878 kb on + strand, within MPMX19_02932 at 186.879 kb on - strand, within MPMX19_02932 at 186.879 kb on - strand, within MPMX19_02932 at 186.879 kb on - strand, within MPMX19_02932 at 186.879 kb on - strand, within MPMX19_02932 at 186.879 kb on - strand, within MPMX19_02932 at 186.879 kb on - strand, within MPMX19_02932 at 186.879 kb on - strand, within MPMX19_02932 at 186.879 kb on - strand, within MPMX19_02932 at 186.879 kb on - strand, within MPMX19_02932 at 186.879 kb on - strand, within MPMX19_02932 at 186.879 kb on - strand, within MPMX19_02932 at 186.887 kb on + strand, within MPMX19_02932 at 186.896 kb on + strand, within MPMX19_02932 at 186.896 kb on + strand, within MPMX19_02932 at 186.896 kb on + strand, within MPMX19_02932 at 186.896 kb on + strand, within MPMX19_02932 at 186.896 kb on + strand, within MPMX19_02932 at 186.896 kb on + strand, within MPMX19_02932 at 186.897 kb on - strand, within MPMX19_02932 at 186.897 kb on - strand, within MPMX19_02932 at 186.897 kb on - strand, within MPMX19_02932 at 186.897 kb on - strand, within MPMX19_02932 at 186.897 kb on - strand, within MPMX19_02932 at 186.897 kb on - strand, within MPMX19_02932 at 186.897 kb on - strand, within MPMX19_02932 at 186.897 kb on - strand, within MPMX19_02932 at 186.897 kb on - strand, within MPMX19_02932 at 186.897 kb on - strand, within MPMX19_02932 at 186.897 kb on - strand, within MPMX19_02932 at 186.965 kb on + strand, within MPMX19_02932 at 186.965 kb on + strand, within MPMX19_02932 at 186.965 kb on + strand, within MPMX19_02932 at 186.965 kb on + strand, within MPMX19_02932 at 186.965 kb on + strand, within MPMX19_02932 at 186.966 kb on - strand, within MPMX19_02932 at 186.966 kb on - strand, within MPMX19_02932 at 186.966 kb on - strand, within MPMX19_02932 at 186.966 kb on - strand, within MPMX19_02932 at 186.966 kb on - strand, within MPMX19_02932 at 186.966 kb on - strand, within MPMX19_02932 at 186.966 kb on - strand, within MPMX19_02932 at 187.022 kb on + strand, within MPMX19_02932 at 187.022 kb on + strand, within MPMX19_02932 at 187.022 kb on + strand, within MPMX19_02932 at 187.022 kb on + strand, within MPMX19_02932 at 187.022 kb on + strand, within MPMX19_02932 at 187.023 kb on - strand, within MPMX19_02932 at 187.023 kb on - strand, within MPMX19_02932 at 187.023 kb on - strand, within MPMX19_02932 at 187.023 kb on - strand, within MPMX19_02932 at 187.076 kb on + strand, within MPMX19_02932 at 187.076 kb on + strand, within MPMX19_02932 at 187.076 kb on + strand, within MPMX19_02932 at 187.076 kb on + strand, within MPMX19_02932 at 187.076 kb on + strand, within MPMX19_02932 at 187.076 kb on + strand, within MPMX19_02932 at 187.076 kb on + strand, within MPMX19_02932 at 187.076 kb on + strand, within MPMX19_02932 at 187.076 kb on + strand, within MPMX19_02932 at 187.076 kb on + strand, within MPMX19_02932 at 187.076 kb on + strand, within MPMX19_02932 at 187.076 kb on + strand, within MPMX19_02932 at 187.077 kb on - strand, within MPMX19_02932 at 187.077 kb on - strand, within MPMX19_02932 at 187.077 kb on - strand, within MPMX19_02932 at 187.077 kb on - strand, within MPMX19_02932 at 187.077 kb on - strand, within MPMX19_02932 at 187.077 kb on - strand, within MPMX19_02932 at 187.077 kb on - strand, within MPMX19_02932 at 187.130 kb on + strand, within MPMX19_02932 at 187.131 kb on - strand, within MPMX19_02932 at 187.280 kb on + strand, within MPMX19_02932 at 187.280 kb on + strand, within MPMX19_02932 at 187.280 kb on + strand, within MPMX19_02932 at 187.280 kb on + strand, within MPMX19_02932 at 187.281 kb on - strand, within MPMX19_02932 at 187.319 kb on + strand, within MPMX19_02932 at 187.320 kb on - strand, within MPMX19_02932 at 187.379 kb on + strand, within MPMX19_02932 at 187.379 kb on + strand, within MPMX19_02932 at 187.379 kb on + strand, within MPMX19_02932 at 187.379 kb on + strand, within MPMX19_02932 at 187.379 kb on + strand, within MPMX19_02932 at 187.380 kb on - strand, within MPMX19_02932 at 187.380 kb on - strand, within MPMX19_02932 at 187.380 kb on - strand, within MPMX19_02932 at 187.380 kb on - strand, within MPMX19_02932 at 187.457 kb on + strand at 187.457 kb on + strand at 187.457 kb on + strand at 187.458 kb on - strand at 187.458 kb on - strand at 187.458 kb on - strand at 187.554 kb on + strand at 187.581 kb on - strand at 187.621 kb on + strand at 187.621 kb on + strand at 187.690 kb on + strand at 187.690 kb on + strand at 187.690 kb on + strand at 187.691 kb on - strand at 187.691 kb on - strand at 187.723 kb on - strand at 187.841 kb on + strand at 187.841 kb on + strand at 187.841 kb on + strand at 187.841 kb on + strand at 187.842 kb on + strand at 187.842 kb on - strand at 187.842 kb on - strand at 187.842 kb on - strand at 187.988 kb on + strand at 187.988 kb on + strand at 187.989 kb on - strand at 187.989 kb on - strand at 188.068 kb on + strand at 188.069 kb on - strand at 188.069 kb on - strand at 188.083 kb on + strand at 188.083 kb on + strand at 188.083 kb on + strand at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.203 kb on + strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.204 kb on - strand, within MPMX19_02933 at 188.233 kb on + strand, within MPMX19_02933 at 188.233 kb on + strand, within MPMX19_02933 at 188.233 kb on + strand, within MPMX19_02933 at 188.233 kb on + strand, within MPMX19_02933 at 188.233 kb on + strand, within MPMX19_02933 at 188.234 kb on - strand, within MPMX19_02933 at 188.275 kb on + strand, within MPMX19_02933 at 188.275 kb on + strand, within MPMX19_02933 at 188.275 kb on + strand, within MPMX19_02933 at 188.275 kb on + strand, within MPMX19_02933 at 188.276 kb on - strand, within MPMX19_02933 at 188.276 kb on - strand, within MPMX19_02933 at 188.276 kb on - strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.497 kb on + strand, within MPMX19_02933 at 188.498 kb on - strand, within MPMX19_02933 at 188.498 kb on - strand, within MPMX19_02933 at 188.498 kb on - strand, within MPMX19_02933 at 188.498 kb on - strand, within MPMX19_02933 at 188.498 kb on - strand, within MPMX19_02933 at 188.498 kb on - strand, within MPMX19_02933 at 188.498 kb on - strand, within MPMX19_02933 at 188.498 kb on - strand, within MPMX19_02933 at 188.498 kb on - strand, within MPMX19_02933
Per-strain Table
Position Strand Gene LocusTag Fraction NF remove 185,916 + -1.1 185,916 + -1.1 185,916 + -1.6 185,916 + -0.8 185,916 + +0.5 185,916 + +0.7 185,916 + -0.3 185,917 - -0.9 185,917 - +0.3 185,917 - -0.3 185,917 - +0.2 185,917 - +0.2 185,917 - -1.2 185,917 - -0.3 185,917 - +0.9 185,917 - -0.3 185,917 - -0.3 185,917 - +1.0 185,917 - -1.1 185,917 - -0.7 185,917 - +0.5 185,917 - -1.4 186,123 + MPMX19_02931 0.27 -1.5 186,123 + MPMX19_02931 0.27 -1.0 186,124 - MPMX19_02931 0.27 -0.8 186,124 - MPMX19_02931 0.27 +0.4 186,261 + MPMX19_02931 0.44 -0.7 186,262 - MPMX19_02931 0.44 -0.1 186,262 - MPMX19_02931 0.44 -0.7 186,262 - MPMX19_02931 0.44 +0.2 186,394 + MPMX19_02931 0.61 -0.8 186,713 + -1.0 186,713 + -0.7 186,713 + -1.0 186,713 + -0.7 186,713 + +0.7 186,713 + -0.2 186,713 + -0.9 186,714 - -0.7 186,714 - +0.2 186,714 - -1.1 186,714 - -2.4 186,719 + -2.9 186,719 + +0.7 186,720 - -0.9 186,720 - -0.8 186,870 - MPMX19_02932 0.21 +2.7 186,870 - MPMX19_02932 0.21 -1.1 186,878 + MPMX19_02932 0.22 +0.2 186,878 + MPMX19_02932 0.22 +0.4 186,878 + MPMX19_02932 0.22 -1.9 186,878 + MPMX19_02932 0.22 -1.3 186,878 + MPMX19_02932 0.22 +1.3 186,878 + MPMX19_02932 0.22 -1.7 186,878 + MPMX19_02932 0.22 +0.2 186,878 + MPMX19_02932 0.22 -1.3 186,878 + MPMX19_02932 0.22 -0.2 186,878 + MPMX19_02932 0.22 -0.6 186,878 + MPMX19_02932 0.22 -0.9 186,879 - MPMX19_02932 0.22 -1.5 186,879 - MPMX19_02932 0.22 -0.9 186,879 - MPMX19_02932 0.22 -0.8 186,879 - MPMX19_02932 0.22 +0.7 186,879 - MPMX19_02932 0.22 -0.3 186,879 - MPMX19_02932 0.22 -0.8 186,879 - MPMX19_02932 0.22 -0.1 186,879 - MPMX19_02932 0.22 +0.7 186,879 - MPMX19_02932 0.22 +0.2 186,879 - MPMX19_02932 0.22 -1.0 186,879 - MPMX19_02932 0.22 -0.2 186,887 + MPMX19_02932 0.23 -1.0 186,896 + MPMX19_02932 0.24 -1.3 186,896 + MPMX19_02932 0.24 -1.1 186,896 + MPMX19_02932 0.24 +0.3 186,896 + MPMX19_02932 0.24 +0.4 186,896 + MPMX19_02932 0.24 +0.1 186,896 + MPMX19_02932 0.24 +0.1 186,897 - MPMX19_02932 0.24 -0.8 186,897 - MPMX19_02932 0.24 -0.5 186,897 - MPMX19_02932 0.24 +2.0 186,897 - MPMX19_02932 0.24 +1.7 186,897 - MPMX19_02932 0.24 -1.8 186,897 - MPMX19_02932 0.24 -0.1 186,897 - MPMX19_02932 0.24 +0.3 186,897 - MPMX19_02932 0.24 -1.5 186,897 - MPMX19_02932 0.24 +0.1 186,897 - MPMX19_02932 0.24 -1.8 186,897 - MPMX19_02932 0.24 -0.8 186,965 + MPMX19_02932 0.33 -0.7 186,965 + MPMX19_02932 0.33 -1.5 186,965 + MPMX19_02932 0.33 +1.5 186,965 + MPMX19_02932 0.33 -1.0 186,965 + MPMX19_02932 0.33 -0.6 186,966 - MPMX19_02932 0.33 -1.9 186,966 - MPMX19_02932 0.33 -0.2 186,966 - MPMX19_02932 0.33 -1.0 186,966 - MPMX19_02932 0.33 +0.7 186,966 - MPMX19_02932 0.33 -0.9 186,966 - MPMX19_02932 0.33 -0.9 186,966 - MPMX19_02932 0.33 -0.6 187,022 + MPMX19_02932 0.40 -0.7 187,022 + MPMX19_02932 0.40 -0.8 187,022 + MPMX19_02932 0.40 +0.1 187,022 + MPMX19_02932 0.40 -0.3 187,022 + MPMX19_02932 0.40 -1.0 187,023 - MPMX19_02932 0.40 -2.8 187,023 - MPMX19_02932 0.40 +0.7 187,023 - MPMX19_02932 0.40 +0.0 187,023 - MPMX19_02932 0.40 -2.0 187,076 + MPMX19_02932 0.46 -0.7 187,076 + MPMX19_02932 0.46 +0.1 187,076 + MPMX19_02932 0.46 -1.0 187,076 + MPMX19_02932 0.46 -0.9 187,076 + MPMX19_02932 0.46 +0.3 187,076 + MPMX19_02932 0.46 +0.5 187,076 + MPMX19_02932 0.46 -1.9 187,076 + MPMX19_02932 0.46 -2.1 187,076 + MPMX19_02932 0.46 -0.9 187,076 + MPMX19_02932 0.46 -0.6 187,076 + MPMX19_02932 0.46 -0.7 187,076 + MPMX19_02932 0.46 -1.0 187,077 - MPMX19_02932 0.46 -1.8 187,077 - MPMX19_02932 0.46 -1.1 187,077 - MPMX19_02932 0.46 -1.1 187,077 - MPMX19_02932 0.46 -0.3 187,077 - MPMX19_02932 0.46 +0.2 187,077 - MPMX19_02932 0.46 -1.0 187,077 - MPMX19_02932 0.46 -0.8 187,130 + MPMX19_02932 0.53 -0.2 187,131 - MPMX19_02932 0.53 -0.1 187,280 + MPMX19_02932 0.71 -1.0 187,280 + MPMX19_02932 0.71 -1.0 187,280 + MPMX19_02932 0.71 +0.4 187,280 + MPMX19_02932 0.71 -0.1 187,281 - MPMX19_02932 0.71 -0.7 187,319 + MPMX19_02932 0.76 -1.9 187,320 - MPMX19_02932 0.76 -0.9 187,379 + MPMX19_02932 0.83 -2.7 187,379 + MPMX19_02932 0.83 -0.6 187,379 + MPMX19_02932 0.83 +0.7 187,379 + MPMX19_02932 0.83 -0.7 187,379 + MPMX19_02932 0.83 +0.3 187,380 - MPMX19_02932 0.84 -0.5 187,380 - MPMX19_02932 0.84 -1.5 187,380 - MPMX19_02932 0.84 +0.3 187,380 - MPMX19_02932 0.84 -0.5 187,457 + -0.1 187,457 + +0.3 187,457 + +0.2 187,458 - +0.1 187,458 - +0.7 187,458 - +0.1 187,554 + -1.6 187,581 - -0.6 187,621 + -2.1 187,621 + +0.1 187,690 + +0.9 187,690 + +0.4 187,690 + +0.9 187,691 - -0.7 187,691 - -0.9 187,723 - -3.1 187,841 + +0.3 187,841 + -0.2 187,841 + -2.1 187,841 + -0.3 187,842 + -0.0 187,842 - -1.2 187,842 - +2.8 187,842 - -1.2 187,988 + -1.5 187,988 + +0.4 187,989 - +0.3 187,989 - -1.9 188,068 + -0.9 188,069 - -2.1 188,069 - -1.7 188,083 + +0.6 188,083 + -1.1 188,083 + -0.5 188,203 + MPMX19_02933 0.15 -1.5 188,203 + MPMX19_02933 0.15 -0.1 188,203 + MPMX19_02933 0.15 -0.8 188,203 + MPMX19_02933 0.15 +0.4 188,203 + MPMX19_02933 0.15 -0.8 188,203 + MPMX19_02933 0.15 +0.1 188,203 + MPMX19_02933 0.15 -0.7 188,203 + MPMX19_02933 0.15 -0.2 188,203 + MPMX19_02933 0.15 -1.8 188,203 + MPMX19_02933 0.15 -2.3 188,203 + MPMX19_02933 0.15 +0.3 188,203 + MPMX19_02933 0.15 +1.7 188,203 + MPMX19_02933 0.15 -0.7 188,203 + MPMX19_02933 0.15 +1.3 188,203 + MPMX19_02933 0.15 +0.8 188,203 + MPMX19_02933 0.15 -0.1 188,203 + MPMX19_02933 0.15 -1.1 188,203 + MPMX19_02933 0.15 +0.4 188,204 - MPMX19_02933 0.15 -1.1 188,204 - MPMX19_02933 0.15 -0.7 188,204 - MPMX19_02933 0.15 +1.7 188,204 - MPMX19_02933 0.15 -1.5 188,204 - MPMX19_02933 0.15 -1.6 188,204 - MPMX19_02933 0.15 -1.1 188,204 - MPMX19_02933 0.15 -0.5 188,204 - MPMX19_02933 0.15 -1.8 188,204 - MPMX19_02933 0.15 +0.0 188,204 - MPMX19_02933 0.15 +1.7 188,204 - MPMX19_02933 0.15 -0.5 188,204 - MPMX19_02933 0.15 +0.3 188,204 - MPMX19_02933 0.15 +1.1 188,204 - MPMX19_02933 0.15 +1.5 188,233 + MPMX19_02933 0.17 -1.1 188,233 + MPMX19_02933 0.17 +0.4 188,233 + MPMX19_02933 0.17 +0.8 188,233 + MPMX19_02933 0.17 -0.3 188,233 + MPMX19_02933 0.17 +1.0 188,234 - MPMX19_02933 0.17 -0.9 188,275 + MPMX19_02933 0.19 -1.0 188,275 + MPMX19_02933 0.19 +0.1 188,275 + MPMX19_02933 0.19 -0.3 188,275 + MPMX19_02933 0.19 -1.0 188,276 - MPMX19_02933 0.19 +1.4 188,276 - MPMX19_02933 0.19 -0.8 188,276 - MPMX19_02933 0.19 -0.6 188,497 + MPMX19_02933 0.31 -0.3 188,497 + MPMX19_02933 0.31 -0.0 188,497 + MPMX19_02933 0.31 +0.2 188,497 + MPMX19_02933 0.31 -0.8 188,497 + MPMX19_02933 0.31 -1.5 188,497 + MPMX19_02933 0.31 +0.7 188,497 + MPMX19_02933 0.31 -0.9 188,497 + MPMX19_02933 0.31 +0.6 188,497 + MPMX19_02933 0.31 -0.8 188,497 + MPMX19_02933 0.31 +0.4 188,497 + MPMX19_02933 0.31 +0.2 188,497 + MPMX19_02933 0.31 -0.5 188,497 + MPMX19_02933 0.31 +0.7 188,497 + MPMX19_02933 0.31 -0.5 188,498 - MPMX19_02933 0.31 +0.1 188,498 - MPMX19_02933 0.31 -0.6 188,498 - MPMX19_02933 0.31 +0.4 188,498 - MPMX19_02933 0.31 -2.0 188,498 - MPMX19_02933 0.31 -0.7 188,498 - MPMX19_02933 0.31 +0.2 188,498 - MPMX19_02933 0.31 -0.7 188,498 - MPMX19_02933 0.31 -0.5 188,498 - MPMX19_02933 0.31 +0.8
Or see this region's nucleotide sequence