Strain Fitness in Escherichia coli ECOR27 around NOLOHH_24425

Experiment: RB49

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrepA and NOLOHH_24430 are separated by 75 nucleotidesNOLOHH_24430 and xerD are separated by 89 nucleotides NOLOHH_24425: repA - RepFIB replication protein A, at 32,016 to 32,993 repA NOLOHH_24430: NOLOHH_24430 - hypothetical protein, at 33,069 to 33,188 _24430 NOLOHH_24435: xerD - recombinase, at 33,278 to 34,018 xerD Position (kb) 32 33Strain fitness (log2 ratio) -2 -1 0 1 2 3at 31.451 kb on + strandat 31.562 kb on + strandat 31.562 kb on + strandat 31.563 kb on - strandat 31.563 kb on - strandat 31.568 kb on + strandat 31.573 kb on - strandat 31.641 kb on + strandat 31.641 kb on + strandat 31.847 kb on + strandat 31.879 kb on + strandat 31.879 kb on + strandat 31.879 kb on + strandat 31.883 kb on + strandat 31.912 kb on + strandat 31.950 kb on + strandat 32.469 kb on + strand, within repAat 32.516 kb on + strand, within repAat 32.527 kb on + strand, within repAat 32.997 kb on + strandat 32.997 kb on + strandat 33.034 kb on - strandat 33.040 kb on + strandat 33.041 kb on - strandat 33.041 kb on - strandat 33.043 kb on - strandat 33.081 kb on - strand, within NOLOHH_24430at 33.083 kb on - strand, within NOLOHH_24430at 33.083 kb on - strand, within NOLOHH_24430at 33.135 kb on + strand, within NOLOHH_24430at 33.135 kb on + strand, within NOLOHH_24430at 33.136 kb on - strand, within NOLOHH_24430at 33.139 kb on + strand, within NOLOHH_24430at 33.139 kb on + strand, within NOLOHH_24430at 33.139 kb on + strand, within NOLOHH_24430at 33.140 kb on - strand, within NOLOHH_24430at 33.140 kb on - strand, within NOLOHH_24430at 33.182 kb on - strandat 33.207 kb on + strandat 33.211 kb on + strandat 33.226 kb on - strandat 33.226 kb on - strandat 33.230 kb on + strandat 33.231 kb on - strandat 33.255 kb on + strandat 33.255 kb on + strandat 33.255 kb on + strandat 33.256 kb on - strandat 33.256 kb on - strandat 33.256 kb on - strandat 33.258 kb on - strandat 33.258 kb on - strandat 33.259 kb on + strandat 33.259 kb on + strandat 33.259 kb on + strandat 33.260 kb on - strandat 33.362 kb on - strand, within xerDat 33.382 kb on + strand, within xerDat 33.383 kb on - strand, within xerDat 33.384 kb on + strand, within xerDat 33.385 kb on - strand, within xerDat 33.470 kb on - strand, within xerDat 33.471 kb on + strand, within xerDat 33.471 kb on + strand, within xerDat 33.471 kb on + strand, within xerDat 33.471 kb on + strand, within xerDat 33.471 kb on + strand, within xerDat 33.471 kb on + strand, within xerDat 33.471 kb on + strand, within xerDat 33.472 kb on - strand, within xerDat 33.472 kb on - strand, within xerDat 33.472 kb on - strand, within xerDat 33.472 kb on - strand, within xerDat 33.472 kb on - strand, within xerDat 33.472 kb on - strand, within xerDat 33.472 kb on - strand, within xerDat 33.645 kb on + strand, within xerDat 33.646 kb on - strand, within xerDat 33.646 kb on - strand, within xerDat 33.650 kb on + strand, within xerDat 33.650 kb on + strand, within xerDat 33.650 kb on + strand, within xerDat 33.650 kb on + strand, within xerDat 33.650 kb on + strand, within xerDat 33.651 kb on - strand, within xerDat 33.651 kb on - strand, within xerDat 33.651 kb on - strand, within xerDat 33.651 kb on - strand, within xerDat 33.651 kb on - strand, within xerDat 33.653 kb on - strand, within xerDat 33.653 kb on - strand, within xerDat 33.744 kb on - strand, within xerDat 33.793 kb on + strand, within xerDat 33.829 kb on + strand, within xerDat 33.851 kb on - strand, within xerDat 33.852 kb on + strand, within xerDat 33.853 kb on - strand, within xerDat 33.853 kb on - strand, within xerDat 33.888 kb on + strand, within xerDat 33.888 kb on + strand, within xerDat 33.888 kb on + strand, within xerD

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Per-strain Table

Position Strand Gene LocusTag Fraction RB49
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31,451 + +1.3
31,562 + +0.8
31,562 + +1.3
31,563 - +0.3
31,563 - +1.3
31,568 + +0.3
31,573 - -0.3
31,641 + +0.7
31,641 + -2.1
31,847 + -1.1
31,879 + +0.3
31,879 + -0.3
31,879 + +0.4
31,883 + -0.1
31,912 + +0.8
31,950 + +0.3
32,469 + repA NOLOHH_24425 0.46 +1.3
32,516 + repA NOLOHH_24425 0.51 -0.3
32,527 + repA NOLOHH_24425 0.52 +1.3
32,997 + -1.1
32,997 + +1.8
33,034 - +1.7
33,040 + +0.8
33,041 - +1.3
33,041 - +0.8
33,043 - -0.2
33,081 - NOLOHH_24430 0.10 +0.3
33,083 - NOLOHH_24430 0.12 +0.5
33,083 - NOLOHH_24430 0.12 +1.3
33,135 + NOLOHH_24430 0.55 +0.3
33,135 + NOLOHH_24430 0.55 +1.3
33,136 - NOLOHH_24430 0.56 +1.0
33,139 + NOLOHH_24430 0.58 -0.7
33,139 + NOLOHH_24430 0.58 -0.7
33,139 + NOLOHH_24430 0.58 -0.7
33,140 - NOLOHH_24430 0.59 +0.3
33,140 - NOLOHH_24430 0.59 +2.3
33,182 - -0.3
33,207 + -0.6
33,211 + +0.3
33,226 - +0.3
33,226 - -1.1
33,230 + +0.3
33,231 - -0.6
33,255 + -0.3
33,255 + -0.1
33,255 + -0.9
33,256 - -1.1
33,256 - +1.0
33,256 - -0.9
33,258 - +0.0
33,258 - -1.6
33,259 + -1.9
33,259 + +0.3
33,259 + -1.3
33,260 - +1.3
33,362 - xerD NOLOHH_24435 0.11 +1.3
33,382 + xerD NOLOHH_24435 0.14 -0.1
33,383 - xerD NOLOHH_24435 0.14 +2.0
33,384 + xerD NOLOHH_24435 0.14 +0.8
33,385 - xerD NOLOHH_24435 0.14 -0.1
33,470 - xerD NOLOHH_24435 0.26 +3.6
33,471 + xerD NOLOHH_24435 0.26 -0.1
33,471 + xerD NOLOHH_24435 0.26 +0.3
33,471 + xerD NOLOHH_24435 0.26 +0.4
33,471 + xerD NOLOHH_24435 0.26 +0.7
33,471 + xerD NOLOHH_24435 0.26 +0.7
33,471 + xerD NOLOHH_24435 0.26 -1.6
33,471 + xerD NOLOHH_24435 0.26 -0.3
33,472 - xerD NOLOHH_24435 0.26 -0.3
33,472 - xerD NOLOHH_24435 0.26 +0.9
33,472 - xerD NOLOHH_24435 0.26 -1.3
33,472 - xerD NOLOHH_24435 0.26 -0.0
33,472 - xerD NOLOHH_24435 0.26 +0.3
33,472 - xerD NOLOHH_24435 0.26 +1.6
33,472 - xerD NOLOHH_24435 0.26 +1.3
33,645 + xerD NOLOHH_24435 0.50 +0.7
33,646 - xerD NOLOHH_24435 0.50 +1.3
33,646 - xerD NOLOHH_24435 0.50 +1.7
33,650 + xerD NOLOHH_24435 0.50 +0.0
33,650 + xerD NOLOHH_24435 0.50 +1.3
33,650 + xerD NOLOHH_24435 0.50 +0.9
33,650 + xerD NOLOHH_24435 0.50 +1.0
33,650 + xerD NOLOHH_24435 0.50 -0.7
33,651 - xerD NOLOHH_24435 0.50 +0.7
33,651 - xerD NOLOHH_24435 0.50 -0.1
33,651 - xerD NOLOHH_24435 0.50 +0.8
33,651 - xerD NOLOHH_24435 0.50 +1.3
33,651 - xerD NOLOHH_24435 0.50 +1.3
33,653 - xerD NOLOHH_24435 0.51 -0.1
33,653 - xerD NOLOHH_24435 0.51 +2.3
33,744 - xerD NOLOHH_24435 0.63 -0.7
33,793 + xerD NOLOHH_24435 0.70 -0.7
33,829 + xerD NOLOHH_24435 0.74 +0.8
33,851 - xerD NOLOHH_24435 0.77 +1.3
33,852 + xerD NOLOHH_24435 0.77 +0.8
33,853 - xerD NOLOHH_24435 0.78 +0.8
33,853 - xerD NOLOHH_24435 0.78 -1.1
33,888 + xerD NOLOHH_24435 0.82 -1.3
33,888 + xerD NOLOHH_24435 0.82 -0.1
33,888 + xerD NOLOHH_24435 0.82 +2.8

Or see this region's nucleotide sequence