Experiment: RB49
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisD and hisG are separated by 5 nucleotides hisG and hisL are separated by 145 nucleotides hisL and yefM are separated by 282 nucleotides yefM and yoeB overlap by 4 nucleotides
NOLOHH_15830: hisD - Histidinol dehydrogenase, at 3,120,789 to 3,122,093
hisD
NOLOHH_15835: hisG - ATP phosphoribosyltransferase, at 3,122,099 to 3,122,998
hisG
NOLOHH_15840: hisL - his operon leader peptide, at 3,123,144 to 3,123,194
hisL
NOLOHH_15845: yefM - YoeB-YefM toxin-antitoxin system antitoxin YefM, at 3,123,477 to 3,123,728
yefM
NOLOHH_15850: yoeB - type II toxin-antitoxin system mRNA interferase toxin YoeB, at 3,123,725 to 3,123,979
yoeB
Position (kb)
3122
3123 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3121.353 kb on - strand, within hisD at 3121.548 kb on - strand, within hisD at 3121.666 kb on - strand, within hisD at 3121.669 kb on - strand, within hisD at 3121.669 kb on - strand, within hisD at 3121.669 kb on - strand, within hisD at 3121.704 kb on - strand, within hisD at 3121.705 kb on + strand, within hisD at 3121.705 kb on + strand, within hisD at 3121.706 kb on - strand, within hisD at 3121.706 kb on - strand, within hisD at 3121.706 kb on - strand, within hisD at 3121.706 kb on - strand, within hisD at 3121.767 kb on + strand, within hisD at 3121.768 kb on - strand, within hisD at 3121.768 kb on - strand, within hisD at 3121.768 kb on - strand, within hisD at 3121.843 kb on - strand, within hisD at 3122.211 kb on - strand, within hisG at 3122.211 kb on - strand, within hisG at 3122.316 kb on + strand, within hisG at 3122.317 kb on - strand, within hisG at 3122.317 kb on - strand, within hisG at 3122.350 kb on + strand, within hisG at 3122.633 kb on + strand, within hisG at 3122.949 kb on - strand at 3123.005 kb on - strand at 3123.015 kb on - strand at 3123.233 kb on - strand at 3123.238 kb on - strand at 3123.272 kb on + strand at 3123.276 kb on + strand at 3123.328 kb on - strand at 3123.363 kb on + strand at 3123.396 kb on - strand at 3123.405 kb on + strand at 3123.416 kb on + strand at 3123.419 kb on + strand at 3123.419 kb on + strand at 3123.419 kb on + strand at 3123.423 kb on + strand at 3123.732 kb on + strand at 3123.732 kb on + strand at 3123.761 kb on + strand, within yoeB at 3123.784 kb on + strand, within yoeB at 3123.784 kb on + strand, within yoeB at 3123.924 kb on + strand, within yoeB at 3123.926 kb on + strand, within yoeB at 3123.993 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB49 remove 3,121,353 - hisD NOLOHH_15830 0.43 -0.7 3,121,548 - hisD NOLOHH_15830 0.58 -0.3 3,121,666 - hisD NOLOHH_15830 0.67 +0.1 3,121,669 - hisD NOLOHH_15830 0.67 -1.0 3,121,669 - hisD NOLOHH_15830 0.67 +1.3 3,121,669 - hisD NOLOHH_15830 0.67 -0.4 3,121,704 - hisD NOLOHH_15830 0.70 +0.6 3,121,705 + hisD NOLOHH_15830 0.70 +1.2 3,121,705 + hisD NOLOHH_15830 0.70 -1.0 3,121,706 - hisD NOLOHH_15830 0.70 -2.0 3,121,706 - hisD NOLOHH_15830 0.70 -2.9 3,121,706 - hisD NOLOHH_15830 0.70 -0.4 3,121,706 - hisD NOLOHH_15830 0.70 +0.9 3,121,767 + hisD NOLOHH_15830 0.75 +0.6 3,121,768 - hisD NOLOHH_15830 0.75 -0.3 3,121,768 - hisD NOLOHH_15830 0.75 +0.0 3,121,768 - hisD NOLOHH_15830 0.75 -0.8 3,121,843 - hisD NOLOHH_15830 0.81 +1.0 3,122,211 - hisG NOLOHH_15835 0.12 +0.8 3,122,211 - hisG NOLOHH_15835 0.12 +0.8 3,122,316 + hisG NOLOHH_15835 0.24 -0.3 3,122,317 - hisG NOLOHH_15835 0.24 -0.4 3,122,317 - hisG NOLOHH_15835 0.24 +0.6 3,122,350 + hisG NOLOHH_15835 0.28 +0.4 3,122,633 + hisG NOLOHH_15835 0.59 -0.4 3,122,949 - +1.2 3,123,005 - +0.6 3,123,015 - -1.1 3,123,233 - +0.5 3,123,238 - -1.0 3,123,272 + -2.0 3,123,276 + +0.3 3,123,328 - -0.4 3,123,363 + -1.7 3,123,396 - +1.6 3,123,405 + -0.5 3,123,416 + -0.4 3,123,419 + -1.0 3,123,419 + +2.2 3,123,419 + +2.2 3,123,423 + -0.4 3,123,732 + +0.9 3,123,732 + -0.3 3,123,761 + yoeB NOLOHH_15850 0.14 +0.2 3,123,784 + yoeB NOLOHH_15850 0.23 +0.0 3,123,784 + yoeB NOLOHH_15850 0.23 +1.1 3,123,924 + yoeB NOLOHH_15850 0.78 +1.3 3,123,926 + yoeB NOLOHH_15850 0.79 +0.3 3,123,993 + -0.4
Or see this region's nucleotide sequence