Experiment: RB49
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NOLOHH_00345 and NOLOHH_00350 overlap by 4 nucleotides NOLOHH_00350 and NOLOHH_00355 are separated by 111 nucleotides NOLOHH_00355 and NOLOHH_00360 are separated by 820 nucleotides
NOLOHH_00345: NOLOHH_00345 - Conjugal transfer protein, at 17,090 to 17,377
_00345
NOLOHH_00350: NOLOHH_00350 - TriA protein, at 17,374 to 17,976
_00350
NOLOHH_00355: NOLOHH_00355 - hypothetical protein, at 18,088 to 18,537
_00355
NOLOHH_00360: NOLOHH_00360 - relaxase/mobilization nuclease, at 19,358 to 20,512
_00360
Position (kb) 18
19 Strain fitness (log2 ratio) -2
-1
0
1 at 17.089 kb on - strand at 17.195 kb on - strand, within NOLOHH_00345 at 17.393 kb on - strand at 17.464 kb on - strand, within NOLOHH_00350 at 17.467 kb on - strand, within NOLOHH_00350 at 17.835 kb on - strand, within NOLOHH_00350 at 17.869 kb on + strand, within NOLOHH_00350 at 17.870 kb on - strand, within NOLOHH_00350 at 17.924 kb on - strand at 17.957 kb on - strand at 17.957 kb on - strand at 18.020 kb on + strand at 18.537 kb on + strand at 18.559 kb on - strand at 18.563 kb on + strand at 18.566 kb on + strand at 18.575 kb on - strand at 18.638 kb on - strand at 18.638 kb on - strand at 18.729 kb on - strand at 18.821 kb on + strand at 18.822 kb on - strand at 18.912 kb on + strand at 18.912 kb on + strand at 18.941 kb on - strand at 18.941 kb on - strand at 18.941 kb on - strand at 18.945 kb on - strand at 18.945 kb on - strand at 18.945 kb on - strand at 18.976 kb on + strand at 18.976 kb on + strand at 18.977 kb on - strand at 18.977 kb on - strand at 18.977 kb on - strand at 18.977 kb on - strand at 18.977 kb on - strand at 18.982 kb on - strand at 19.051 kb on - strand at 19.088 kb on - strand at 19.089 kb on + strand at 19.097 kb on + strand at 19.100 kb on - strand at 19.104 kb on + strand at 19.105 kb on - strand at 19.123 kb on - strand at 19.136 kb on + strand at 19.136 kb on + strand at 19.140 kb on + strand at 19.241 kb on - strand at 19.241 kb on - strand at 19.242 kb on + strand at 19.255 kb on + strand at 19.255 kb on + strand at 19.255 kb on + strand at 19.256 kb on - strand at 19.256 kb on - strand at 19.256 kb on - strand at 19.258 kb on - strand at 19.274 kb on + strand at 19.274 kb on + strand at 19.274 kb on + strand at 19.275 kb on - strand at 19.459 kb on + strand at 19.460 kb on - strand at 19.484 kb on + strand, within NOLOHH_00360 at 19.484 kb on + strand, within NOLOHH_00360 at 19.485 kb on - strand, within NOLOHH_00360 at 19.485 kb on - strand, within NOLOHH_00360 at 19.485 kb on - strand, within NOLOHH_00360 at 19.517 kb on + strand, within NOLOHH_00360 at 19.517 kb on + strand, within NOLOHH_00360 at 19.518 kb on - strand, within NOLOHH_00360 at 19.518 kb on - strand, within NOLOHH_00360
Per-strain Table
Position Strand Gene LocusTag Fraction RB49 remove 17,089 - -0.0 17,195 - NOLOHH_00345 0.36 -0.6 17,393 - -0.6 17,464 - NOLOHH_00350 0.15 -1.0 17,467 - NOLOHH_00350 0.15 -0.5 17,835 - NOLOHH_00350 0.76 -0.4 17,869 + NOLOHH_00350 0.82 -0.0 17,870 - NOLOHH_00350 0.82 +0.6 17,924 - -0.3 17,957 - +0.4 17,957 - +0.7 18,020 + +0.6 18,537 + +0.0 18,559 - +0.3 18,563 + -0.1 18,566 + +0.1 18,575 - +0.1 18,638 - +0.1 18,638 - -0.2 18,729 - -0.6 18,821 + -0.8 18,822 - -0.1 18,912 + -0.7 18,912 + -0.3 18,941 - +0.3 18,941 - -0.2 18,941 - +0.1 18,945 - -1.0 18,945 - +0.8 18,945 - +0.3 18,976 + -0.5 18,976 + +0.8 18,977 - +0.7 18,977 - -0.5 18,977 - -0.2 18,977 - +1.0 18,977 - -0.0 18,982 - -0.3 19,051 - -0.8 19,088 - -1.7 19,089 + -0.3 19,097 + +0.5 19,100 - -1.0 19,104 + -0.5 19,105 - -0.7 19,123 - -1.2 19,136 + +0.4 19,136 + -0.7 19,140 + +0.2 19,241 - -1.5 19,241 - -1.8 19,242 + -0.4 19,255 + -0.7 19,255 + -0.2 19,255 + -0.0 19,256 - -0.3 19,256 - +0.6 19,256 - -2.3 19,258 - -0.4 19,274 + +0.7 19,274 + -0.2 19,274 + -0.4 19,275 - -0.7 19,459 + -0.5 19,460 - -1.2 19,484 + NOLOHH_00360 0.11 -1.4 19,484 + NOLOHH_00360 0.11 -0.6 19,485 - NOLOHH_00360 0.11 -0.8 19,485 - NOLOHH_00360 0.11 -2.2 19,485 - NOLOHH_00360 0.11 -1.0 19,517 + NOLOHH_00360 0.14 -0.3 19,517 + NOLOHH_00360 0.14 -2.3 19,518 - NOLOHH_00360 0.14 -1.1 19,518 - NOLOHH_00360 0.14 -2.2
Or see this region's nucleotide sequence