Experiment: NF
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX19_04731 and MPMX19_04732 overlap by 8 nucleotides MPMX19_04732 and MPMX19_04733 are separated by 100 nucleotides MPMX19_04733 and MPMX19_04734 overlap by 22 nucleotides MPMX19_04734 and MPMX19_04735 are separated by 17 nucleotides
MPMX19_04731: MPMX19_04731 - hypothetical protein, at 122,581 to 123,630
_04731
MPMX19_04732: MPMX19_04732 - hypothetical protein, at 123,623 to 124,513
_04732
MPMX19_04733: MPMX19_04733 - hypothetical protein, at 124,614 to 125,357
_04733
MPMX19_04734: MPMX19_04734 - 5,6,7,8-tetrahydromethanopterin hydro-lyase, at 125,336 to 125,701
_04734
MPMX19_04735: MPMX19_04735 - hypothetical protein, at 125,719 to 126,795
_04735
Position (kb)
124
125
126 Strain fitness (log2 ratio)
-2
-1
0
1 at 123.726 kb on + strand, within MPMX19_04732 at 123.816 kb on + strand, within MPMX19_04732 at 123.816 kb on + strand, within MPMX19_04732 at 123.816 kb on + strand, within MPMX19_04732 at 123.816 kb on + strand, within MPMX19_04732 at 123.816 kb on + strand, within MPMX19_04732 at 123.816 kb on + strand, within MPMX19_04732 at 123.817 kb on - strand, within MPMX19_04732 at 123.817 kb on - strand, within MPMX19_04732 at 123.817 kb on - strand, within MPMX19_04732 at 124.578 kb on + strand at 124.578 kb on + strand at 124.578 kb on + strand at 124.578 kb on + strand at 124.578 kb on + strand at 124.578 kb on + strand at 124.579 kb on - strand at 124.579 kb on - strand at 124.579 kb on - strand at 124.579 kb on - strand at 124.579 kb on - strand at 124.579 kb on - strand at 124.579 kb on - strand at 124.734 kb on - strand, within MPMX19_04733 at 124.776 kb on + strand, within MPMX19_04733 at 124.776 kb on + strand, within MPMX19_04733 at 124.776 kb on + strand, within MPMX19_04733 at 124.776 kb on + strand, within MPMX19_04733 at 124.776 kb on + strand, within MPMX19_04733 at 124.776 kb on + strand, within MPMX19_04733 at 124.776 kb on + strand, within MPMX19_04733 at 124.776 kb on + strand, within MPMX19_04733 at 124.776 kb on + strand, within MPMX19_04733 at 124.776 kb on + strand, within MPMX19_04733 at 124.776 kb on + strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.777 kb on - strand, within MPMX19_04733 at 124.912 kb on + strand, within MPMX19_04733 at 125.448 kb on + strand, within MPMX19_04734 at 125.448 kb on + strand, within MPMX19_04734 at 125.448 kb on + strand, within MPMX19_04734 at 125.448 kb on + strand, within MPMX19_04734 at 125.448 kb on + strand, within MPMX19_04734 at 125.448 kb on + strand, within MPMX19_04734 at 125.448 kb on + strand, within MPMX19_04734 at 125.449 kb on - strand, within MPMX19_04734 at 125.449 kb on - strand, within MPMX19_04734 at 125.449 kb on - strand, within MPMX19_04734 at 125.449 kb on - strand, within MPMX19_04734 at 125.449 kb on - strand, within MPMX19_04734 at 125.824 kb on + strand at 125.824 kb on + strand at 125.824 kb on + strand at 125.824 kb on + strand at 125.824 kb on + strand at 125.825 kb on - strand at 125.825 kb on - strand at 125.825 kb on - strand at 125.840 kb on - strand, within MPMX19_04735 at 125.840 kb on - strand, within MPMX19_04735 at 126.272 kb on + strand, within MPMX19_04735 at 126.272 kb on + strand, within MPMX19_04735 at 126.272 kb on + strand, within MPMX19_04735 at 126.272 kb on + strand, within MPMX19_04735 at 126.273 kb on - strand, within MPMX19_04735 at 126.273 kb on - strand, within MPMX19_04735 at 126.273 kb on - strand, within MPMX19_04735 at 126.273 kb on - strand, within MPMX19_04735 at 126.273 kb on - strand, within MPMX19_04735
Per-strain Table
Position Strand Gene LocusTag Fraction NF remove 123,726 + MPMX19_04732 0.12 -0.6 123,816 + MPMX19_04732 0.22 +0.7 123,816 + MPMX19_04732 0.22 +0.5 123,816 + MPMX19_04732 0.22 +0.7 123,816 + MPMX19_04732 0.22 -0.9 123,816 + MPMX19_04732 0.22 -0.0 123,816 + MPMX19_04732 0.22 +1.0 123,817 - MPMX19_04732 0.22 -0.3 123,817 - MPMX19_04732 0.22 -1.6 123,817 - MPMX19_04732 0.22 +0.6 124,578 + -0.0 124,578 + -0.9 124,578 + -0.5 124,578 + -0.7 124,578 + +0.2 124,578 + -0.5 124,579 - -0.4 124,579 - -0.1 124,579 - -1.0 124,579 - -1.8 124,579 - +0.3 124,579 - -1.6 124,579 - -1.0 124,734 - MPMX19_04733 0.16 -0.1 124,776 + MPMX19_04733 0.22 -1.2 124,776 + MPMX19_04733 0.22 +0.7 124,776 + MPMX19_04733 0.22 -0.0 124,776 + MPMX19_04733 0.22 -0.6 124,776 + MPMX19_04733 0.22 -0.1 124,776 + MPMX19_04733 0.22 -0.2 124,776 + MPMX19_04733 0.22 +1.2 124,776 + MPMX19_04733 0.22 -0.3 124,776 + MPMX19_04733 0.22 -0.3 124,776 + MPMX19_04733 0.22 +0.6 124,776 + MPMX19_04733 0.22 -1.1 124,777 - MPMX19_04733 0.22 +1.7 124,777 - MPMX19_04733 0.22 +0.5 124,777 - MPMX19_04733 0.22 -1.3 124,777 - MPMX19_04733 0.22 -0.6 124,777 - MPMX19_04733 0.22 -1.0 124,777 - MPMX19_04733 0.22 -0.0 124,777 - MPMX19_04733 0.22 -1.6 124,777 - MPMX19_04733 0.22 -1.2 124,777 - MPMX19_04733 0.22 +0.8 124,777 - MPMX19_04733 0.22 +0.2 124,777 - MPMX19_04733 0.22 -1.4 124,777 - MPMX19_04733 0.22 +0.6 124,777 - MPMX19_04733 0.22 -1.0 124,777 - MPMX19_04733 0.22 -0.9 124,912 + MPMX19_04733 0.40 +0.7 125,448 + MPMX19_04734 0.31 -0.4 125,448 + MPMX19_04734 0.31 +0.2 125,448 + MPMX19_04734 0.31 -1.4 125,448 + MPMX19_04734 0.31 +0.7 125,448 + MPMX19_04734 0.31 -0.5 125,448 + MPMX19_04734 0.31 +0.2 125,448 + MPMX19_04734 0.31 -0.6 125,449 - MPMX19_04734 0.31 -1.8 125,449 - MPMX19_04734 0.31 -1.2 125,449 - MPMX19_04734 0.31 +0.4 125,449 - MPMX19_04734 0.31 +0.3 125,449 - MPMX19_04734 0.31 -0.3 125,824 + -0.3 125,824 + -1.2 125,824 + -0.6 125,824 + +0.6 125,824 + -0.5 125,825 - -0.7 125,825 - +0.3 125,825 - -0.0 125,840 - MPMX19_04735 0.11 -0.6 125,840 - MPMX19_04735 0.11 -0.7 126,272 + MPMX19_04735 0.51 +0.7 126,272 + MPMX19_04735 0.51 -0.1 126,272 + MPMX19_04735 0.51 -1.2 126,272 + MPMX19_04735 0.51 -2.5 126,273 - MPMX19_04735 0.51 +0.4 126,273 - MPMX19_04735 0.51 -1.4 126,273 - MPMX19_04735 0.51 +0.4 126,273 - MPMX19_04735 0.51 -1.1 126,273 - MPMX19_04735 0.51 +0.2
Or see this region's nucleotide sequence