Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rutB and rutC are separated by 11 nucleotides rutC and rutD are separated by 7 nucleotides rutD and rutE are separated by 9 nucleotides rutE and rutF are separated by 10 nucleotides
NOLOHH_21520: rutB - peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB, at 4,230,950 to 4,231,645
rutB
NOLOHH_21525: rutC - pyrimidine utilization protein C, at 4,231,657 to 4,232,043
rutC
NOLOHH_21530: rutD - pyrimidine utilization protein D, at 4,232,051 to 4,232,851
rutD
NOLOHH_21535: rutE - malonic semialdehyde reductase, at 4,232,861 to 4,233,451
rutE
NOLOHH_21540: rutF - malonic semialdehyde reductase, at 4,233,462 to 4,233,956
rutF
Position (kb)
4232
4233 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4231.060 kb on - strand, within rutB at 4231.184 kb on + strand, within rutB at 4231.184 kb on + strand, within rutB at 4231.185 kb on - strand, within rutB at 4231.249 kb on + strand, within rutB at 4231.250 kb on - strand, within rutB at 4231.375 kb on + strand, within rutB at 4231.375 kb on + strand, within rutB at 4231.410 kb on + strand, within rutB at 4231.487 kb on + strand, within rutB at 4231.487 kb on + strand, within rutB at 4231.670 kb on + strand at 4231.675 kb on - strand at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.741 kb on + strand, within rutC at 4231.742 kb on - strand, within rutC at 4231.742 kb on - strand, within rutC at 4231.742 kb on - strand, within rutC at 4231.742 kb on - strand, within rutC at 4231.742 kb on - strand, within rutC at 4231.742 kb on - strand, within rutC at 4231.773 kb on + strand, within rutC at 4231.773 kb on + strand, within rutC at 4231.773 kb on + strand, within rutC at 4231.773 kb on + strand, within rutC at 4231.774 kb on - strand, within rutC at 4231.774 kb on - strand, within rutC at 4231.774 kb on - strand, within rutC at 4232.206 kb on + strand, within rutD at 4232.231 kb on + strand, within rutD at 4232.232 kb on - strand, within rutD at 4232.442 kb on + strand, within rutD at 4232.448 kb on - strand, within rutD at 4232.584 kb on - strand, within rutD at 4232.827 kb on - strand at 4232.827 kb on - strand at 4232.860 kb on + strand at 4232.970 kb on - strand, within rutE at 4232.970 kb on - strand, within rutE at 4233.002 kb on - strand, within rutE at 4233.002 kb on - strand, within rutE at 4233.039 kb on + strand, within rutE at 4233.040 kb on - strand, within rutE at 4233.317 kb on + strand, within rutE at 4233.317 kb on + strand, within rutE at 4233.317 kb on + strand, within rutE at 4233.317 kb on + strand, within rutE at 4233.318 kb on - strand, within rutE at 4233.318 kb on - strand, within rutE at 4233.318 kb on - strand, within rutE at 4233.318 kb on - strand, within rutE at 4233.449 kb on + strand at 4233.450 kb on - strand at 4233.461 kb on + strand at 4233.461 kb on + strand at 4233.461 kb on + strand at 4233.469 kb on + strand at 4233.469 kb on + strand at 4233.470 kb on - strand at 4233.470 kb on - strand at 4233.607 kb on + strand, within rutF at 4233.678 kb on - strand, within rutF
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 4,231,060 - rutB NOLOHH_21520 0.16 +0.8 4,231,184 + rutB NOLOHH_21520 0.34 -1.9 4,231,184 + rutB NOLOHH_21520 0.34 +1.3 4,231,185 - rutB NOLOHH_21520 0.34 -1.9 4,231,249 + rutB NOLOHH_21520 0.43 +0.4 4,231,250 - rutB NOLOHH_21520 0.43 -1.6 4,231,375 + rutB NOLOHH_21520 0.61 +1.2 4,231,375 + rutB NOLOHH_21520 0.61 +0.4 4,231,410 + rutB NOLOHH_21520 0.66 +0.4 4,231,487 + rutB NOLOHH_21520 0.77 +0.4 4,231,487 + rutB NOLOHH_21520 0.77 +0.1 4,231,670 + -0.1 4,231,675 - -0.3 4,231,741 + rutC NOLOHH_21525 0.22 +0.5 4,231,741 + rutC NOLOHH_21525 0.22 -0.0 4,231,741 + rutC NOLOHH_21525 0.22 +0.5 4,231,741 + rutC NOLOHH_21525 0.22 +0.7 4,231,741 + rutC NOLOHH_21525 0.22 -0.7 4,231,741 + rutC NOLOHH_21525 0.22 +0.5 4,231,741 + rutC NOLOHH_21525 0.22 -0.5 4,231,741 + rutC NOLOHH_21525 0.22 -0.2 4,231,742 - rutC NOLOHH_21525 0.22 +1.5 4,231,742 - rutC NOLOHH_21525 0.22 +1.5 4,231,742 - rutC NOLOHH_21525 0.22 +0.1 4,231,742 - rutC NOLOHH_21525 0.22 -0.5 4,231,742 - rutC NOLOHH_21525 0.22 +0.9 4,231,742 - rutC NOLOHH_21525 0.22 -0.8 4,231,773 + rutC NOLOHH_21525 0.30 +0.0 4,231,773 + rutC NOLOHH_21525 0.30 -1.5 4,231,773 + rutC NOLOHH_21525 0.30 +0.0 4,231,773 + rutC NOLOHH_21525 0.30 -0.1 4,231,774 - rutC NOLOHH_21525 0.30 -0.5 4,231,774 - rutC NOLOHH_21525 0.30 +0.5 4,231,774 - rutC NOLOHH_21525 0.30 +0.7 4,232,206 + rutD NOLOHH_21530 0.19 +0.5 4,232,231 + rutD NOLOHH_21530 0.22 -0.5 4,232,232 - rutD NOLOHH_21530 0.23 +0.5 4,232,442 + rutD NOLOHH_21530 0.49 -0.5 4,232,448 - rutD NOLOHH_21530 0.50 -0.8 4,232,584 - rutD NOLOHH_21530 0.67 -0.5 4,232,827 - -0.5 4,232,827 - +1.5 4,232,860 + -0.9 4,232,970 - rutE NOLOHH_21535 0.18 -0.7 4,232,970 - rutE NOLOHH_21535 0.18 -0.4 4,233,002 - rutE NOLOHH_21535 0.24 +1.5 4,233,002 - rutE NOLOHH_21535 0.24 +1.5 4,233,039 + rutE NOLOHH_21535 0.30 -0.7 4,233,040 - rutE NOLOHH_21535 0.30 -1.1 4,233,317 + rutE NOLOHH_21535 0.77 +0.2 4,233,317 + rutE NOLOHH_21535 0.77 +2.8 4,233,317 + rutE NOLOHH_21535 0.77 -0.1 4,233,317 + rutE NOLOHH_21535 0.77 +0.2 4,233,318 - rutE NOLOHH_21535 0.77 +0.5 4,233,318 - rutE NOLOHH_21535 0.77 +1.7 4,233,318 - rutE NOLOHH_21535 0.77 +0.8 4,233,318 - rutE NOLOHH_21535 0.77 -0.1 4,233,449 + +2.1 4,233,450 - -1.5 4,233,461 + +0.2 4,233,461 + +0.8 4,233,461 + -0.5 4,233,469 + +0.5 4,233,469 + -2.5 4,233,470 - +0.5 4,233,470 - +1.5 4,233,607 + rutF NOLOHH_21540 0.29 +0.2 4,233,678 - rutF NOLOHH_21540 0.44 +0.5
Or see this region's nucleotide sequence