Strain Fitness in Escherichia coli ECOR27 around NOLOHH_18950

Experiment: K5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdosC and dosP are separated by 24 nucleotidesdosP and ddpX are separated by 257 nucleotidesddpX and ddpA are separated by 13 nucleotides NOLOHH_18945: dosC - diguanylate cyclase DosC, at 3,721,214 to 3,722,596 dosC NOLOHH_18950: dosP - oxygen-sensing cyclic-di-GMP phosphodiesterase, at 3,722,621 to 3,725,020 dosP NOLOHH_18955: ddpX - D-alanyl-D-alanine dipeptidase, at 3,725,278 to 3,725,859 ddpX NOLOHH_18960: ddpA - putative D,D-dipeptide-binding periplasmic protein DdpA, at 3,725,873 to 3,727,423 ddpA Position (kb) 3722 3723 3724 3725 3726Strain fitness (log2 ratio) -1 0 1 2at 3721.724 kb on - strand, within dosCat 3721.830 kb on + strand, within dosCat 3722.540 kb on + strandat 3722.540 kb on + strandat 3722.594 kb on + strandat 3723.140 kb on - strand, within dosPat 3723.292 kb on + strand, within dosPat 3723.629 kb on - strand, within dosPat 3723.692 kb on - strand, within dosPat 3723.734 kb on - strand, within dosPat 3724.015 kb on + strand, within dosPat 3724.175 kb on - strand, within dosPat 3725.402 kb on + strand, within ddpXat 3725.539 kb on + strand, within ddpXat 3725.539 kb on + strand, within ddpXat 3725.830 kb on - strandat 3725.831 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K5
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3,721,724 - dosC NOLOHH_18945 0.37 -1.2
3,721,830 + dosC NOLOHH_18945 0.45 -0.6
3,722,540 + +0.1
3,722,540 + +0.4
3,722,594 + +2.0
3,723,140 - dosP NOLOHH_18950 0.22 +0.8
3,723,292 + dosP NOLOHH_18950 0.28 +0.7
3,723,629 - dosP NOLOHH_18950 0.42 -0.6
3,723,692 - dosP NOLOHH_18950 0.45 -0.2
3,723,734 - dosP NOLOHH_18950 0.46 +0.0
3,724,015 + dosP NOLOHH_18950 0.58 -0.6
3,724,175 - dosP NOLOHH_18950 0.65 +1.4
3,725,402 + ddpX NOLOHH_18955 0.21 -0.6
3,725,539 + ddpX NOLOHH_18955 0.45 +0.6
3,725,539 + ddpX NOLOHH_18955 0.45 +2.0
3,725,830 - +0.3
3,725,831 + -0.6

Or see this region's nucleotide sequence