Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisD and hisG are separated by 5 nucleotides hisG and hisL are separated by 145 nucleotides hisL and yefM are separated by 282 nucleotides yefM and yoeB overlap by 4 nucleotides
NOLOHH_15830: hisD - Histidinol dehydrogenase, at 3,120,789 to 3,122,093
hisD
NOLOHH_15835: hisG - ATP phosphoribosyltransferase, at 3,122,099 to 3,122,998
hisG
NOLOHH_15840: hisL - his operon leader peptide, at 3,123,144 to 3,123,194
hisL
NOLOHH_15845: yefM - YoeB-YefM toxin-antitoxin system antitoxin YefM, at 3,123,477 to 3,123,728
yefM
NOLOHH_15850: yoeB - type II toxin-antitoxin system mRNA interferase toxin YoeB, at 3,123,725 to 3,123,979
yoeB
Position (kb)
3122
3123 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3121.353 kb on - strand, within hisD at 3121.548 kb on - strand, within hisD at 3121.666 kb on - strand, within hisD at 3121.669 kb on - strand, within hisD at 3121.669 kb on - strand, within hisD at 3121.669 kb on - strand, within hisD at 3121.704 kb on - strand, within hisD at 3121.705 kb on + strand, within hisD at 3121.705 kb on + strand, within hisD at 3121.706 kb on - strand, within hisD at 3121.706 kb on - strand, within hisD at 3121.706 kb on - strand, within hisD at 3121.706 kb on - strand, within hisD at 3121.767 kb on + strand, within hisD at 3121.768 kb on - strand, within hisD at 3121.768 kb on - strand, within hisD at 3121.768 kb on - strand, within hisD at 3121.843 kb on - strand, within hisD at 3122.211 kb on - strand, within hisG at 3122.211 kb on - strand, within hisG at 3122.316 kb on + strand, within hisG at 3122.317 kb on - strand, within hisG at 3122.317 kb on - strand, within hisG at 3122.350 kb on + strand, within hisG at 3122.633 kb on + strand, within hisG at 3122.949 kb on - strand at 3123.005 kb on - strand at 3123.015 kb on - strand at 3123.233 kb on - strand at 3123.238 kb on - strand at 3123.272 kb on + strand at 3123.276 kb on + strand at 3123.328 kb on - strand at 3123.363 kb on + strand at 3123.396 kb on - strand at 3123.405 kb on + strand at 3123.416 kb on + strand at 3123.419 kb on + strand at 3123.419 kb on + strand at 3123.419 kb on + strand at 3123.423 kb on + strand at 3123.732 kb on + strand at 3123.732 kb on + strand at 3123.761 kb on + strand, within yoeB at 3123.784 kb on + strand, within yoeB at 3123.784 kb on + strand, within yoeB at 3123.924 kb on + strand, within yoeB at 3123.926 kb on + strand, within yoeB at 3123.993 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 3,121,353 - hisD NOLOHH_15830 0.43 +0.4 3,121,548 - hisD NOLOHH_15830 0.58 -1.2 3,121,666 - hisD NOLOHH_15830 0.67 -0.8 3,121,669 - hisD NOLOHH_15830 0.67 -0.2 3,121,669 - hisD NOLOHH_15830 0.67 -0.2 3,121,669 - hisD NOLOHH_15830 0.67 +0.4 3,121,704 - hisD NOLOHH_15830 0.70 +0.1 3,121,705 + hisD NOLOHH_15830 0.70 +1.4 3,121,705 + hisD NOLOHH_15830 0.70 -0.2 3,121,706 - hisD NOLOHH_15830 0.70 -0.6 3,121,706 - hisD NOLOHH_15830 0.70 -2.0 3,121,706 - hisD NOLOHH_15830 0.70 -0.6 3,121,706 - hisD NOLOHH_15830 0.70 -1.9 3,121,767 + hisD NOLOHH_15830 0.75 +0.4 3,121,768 - hisD NOLOHH_15830 0.75 -0.4 3,121,768 - hisD NOLOHH_15830 0.75 -0.6 3,121,768 - hisD NOLOHH_15830 0.75 -0.6 3,121,843 - hisD NOLOHH_15830 0.81 -0.6 3,122,211 - hisG NOLOHH_15835 0.12 +1.8 3,122,211 - hisG NOLOHH_15835 0.12 +0.2 3,122,316 + hisG NOLOHH_15835 0.24 -0.1 3,122,317 - hisG NOLOHH_15835 0.24 -2.6 3,122,317 - hisG NOLOHH_15835 0.24 +1.2 3,122,350 + hisG NOLOHH_15835 0.28 -0.3 3,122,633 + hisG NOLOHH_15835 0.59 -0.6 3,122,949 - +0.2 3,123,005 - +0.4 3,123,015 - +1.0 3,123,233 - +0.5 3,123,238 - +1.4 3,123,272 + -0.6 3,123,276 + +1.1 3,123,328 - +1.0 3,123,363 + +0.4 3,123,396 - +2.0 3,123,405 + -0.3 3,123,416 + -0.3 3,123,419 + +0.2 3,123,419 + +0.4 3,123,419 + +2.4 3,123,423 + -0.6 3,123,732 + +0.0 3,123,732 + -1.4 3,123,761 + yoeB NOLOHH_15850 0.14 +1.2 3,123,784 + yoeB NOLOHH_15850 0.23 +2.0 3,123,784 + yoeB NOLOHH_15850 0.23 +1.5 3,123,924 + yoeB NOLOHH_15850 0.78 +0.1 3,123,926 + yoeB NOLOHH_15850 0.79 +1.4 3,123,993 + +1.0
Or see this region's nucleotide sequence