Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisA and hisH overlap by 1 nucleotides hisH and hisB overlap by 1 nucleotides hisB and hisC overlap by 1 nucleotides
NOLOHH_15810: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 3,117,328 to 3,118,065
hisA
NOLOHH_15815: hisH - imidazole glycerol phosphate synthase subunit HisH, at 3,118,065 to 3,118,655
hisH
NOLOHH_15820: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 3,118,655 to 3,119,722
hisB
NOLOHH_15825: hisC - histidinol-phosphate transaminase, at 3,119,722 to 3,120,792
hisC
Position (kb)
3118
3119
3120 Strain fitness (log2 ratio)
-1
0
1 at 3117.695 kb on - strand, within hisA at 3117.871 kb on - strand, within hisA at 3117.871 kb on - strand, within hisA at 3117.962 kb on - strand, within hisA at 3117.962 kb on - strand, within hisA at 3117.962 kb on - strand, within hisA at 3118.139 kb on - strand, within hisH at 3118.184 kb on + strand, within hisH at 3118.184 kb on + strand, within hisH at 3118.185 kb on - strand, within hisH at 3118.185 kb on - strand, within hisH at 3118.185 kb on - strand, within hisH at 3118.237 kb on + strand, within hisH at 3118.766 kb on - strand, within hisB at 3118.880 kb on - strand, within hisB at 3118.880 kb on - strand, within hisB at 3118.880 kb on - strand, within hisB at 3118.907 kb on - strand, within hisB at 3119.050 kb on - strand, within hisB at 3119.141 kb on - strand, within hisB at 3119.346 kb on + strand, within hisB at 3119.346 kb on + strand, within hisB at 3119.347 kb on - strand, within hisB at 3119.741 kb on - strand at 3119.818 kb on - strand at 3119.831 kb on - strand, within hisC at 3119.860 kb on - strand, within hisC at 3119.898 kb on - strand, within hisC at 3119.961 kb on - strand, within hisC at 3120.166 kb on + strand, within hisC at 3120.233 kb on + strand, within hisC at 3120.234 kb on - strand, within hisC at 3120.234 kb on - strand, within hisC at 3120.341 kb on + strand, within hisC at 3120.341 kb on + strand, within hisC at 3120.342 kb on - strand, within hisC at 3120.382 kb on + strand, within hisC at 3120.575 kb on - strand, within hisC
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 3,117,695 - hisA NOLOHH_15810 0.50 +1.7 3,117,871 - hisA NOLOHH_15810 0.74 +1.3 3,117,871 - hisA NOLOHH_15810 0.74 +0.2 3,117,962 - hisA NOLOHH_15810 0.86 -0.6 3,117,962 - hisA NOLOHH_15810 0.86 +1.4 3,117,962 - hisA NOLOHH_15810 0.86 +0.4 3,118,139 - hisH NOLOHH_15815 0.13 -1.0 3,118,184 + hisH NOLOHH_15815 0.20 -0.6 3,118,184 + hisH NOLOHH_15815 0.20 +0.4 3,118,185 - hisH NOLOHH_15815 0.20 -0.6 3,118,185 - hisH NOLOHH_15815 0.20 +1.4 3,118,185 - hisH NOLOHH_15815 0.20 -0.6 3,118,237 + hisH NOLOHH_15815 0.29 +1.4 3,118,766 - hisB NOLOHH_15820 0.10 -0.6 3,118,880 - hisB NOLOHH_15820 0.21 +1.0 3,118,880 - hisB NOLOHH_15820 0.21 -0.9 3,118,880 - hisB NOLOHH_15820 0.21 +1.4 3,118,907 - hisB NOLOHH_15820 0.24 -0.8 3,119,050 - hisB NOLOHH_15820 0.37 +0.7 3,119,141 - hisB NOLOHH_15820 0.46 -1.0 3,119,346 + hisB NOLOHH_15820 0.65 +0.4 3,119,346 + hisB NOLOHH_15820 0.65 +0.4 3,119,347 - hisB NOLOHH_15820 0.65 -0.2 3,119,741 - -0.2 3,119,818 - -0.4 3,119,831 - hisC NOLOHH_15825 0.10 -0.9 3,119,860 - hisC NOLOHH_15825 0.13 -0.0 3,119,898 - hisC NOLOHH_15825 0.16 +1.0 3,119,961 - hisC NOLOHH_15825 0.22 -0.0 3,120,166 + hisC NOLOHH_15825 0.41 +1.4 3,120,233 + hisC NOLOHH_15825 0.48 -0.5 3,120,234 - hisC NOLOHH_15825 0.48 +1.1 3,120,234 - hisC NOLOHH_15825 0.48 +0.1 3,120,341 + hisC NOLOHH_15825 0.58 +0.4 3,120,341 + hisC NOLOHH_15825 0.58 +1.7 3,120,342 - hisC NOLOHH_15825 0.58 +1.1 3,120,382 + hisC NOLOHH_15825 0.62 +0.4 3,120,575 - hisC NOLOHH_15825 0.80 -0.1
Or see this region's nucleotide sequence