Strain Fitness in Escherichia coli ECOR27 around NOLOHH_15660

Experiment: K5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaC and wcaD overlap by 26 nucleotideswcaD and wcaE are separated by 10 nucleotideswcaE and wcaF are separated by 15 nucleotides NOLOHH_15655: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 3,082,016 to 3,083,233 wcaC NOLOHH_15660: wcaD - putative colanic acid polymerase WcaD, at 3,083,208 to 3,084,425 wcaD NOLOHH_15665: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 3,084,436 to 3,085,182 wcaE NOLOHH_15670: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 3,085,198 to 3,085,746 wcaF Position (kb) 3083 3084 3085Strain fitness (log2 ratio) -2 -1 0 1at 3082.504 kb on + strand, within wcaCat 3082.504 kb on + strand, within wcaCat 3082.564 kb on - strand, within wcaCat 3082.597 kb on + strand, within wcaCat 3082.640 kb on + strand, within wcaCat 3082.773 kb on - strand, within wcaCat 3082.849 kb on + strand, within wcaCat 3082.970 kb on + strand, within wcaCat 3083.133 kb on + strandat 3083.208 kb on - strandat 3083.256 kb on + strandat 3083.323 kb on + strandat 3083.412 kb on - strand, within wcaDat 3083.415 kb on + strand, within wcaDat 3083.448 kb on + strand, within wcaDat 3083.449 kb on - strand, within wcaDat 3083.476 kb on - strand, within wcaDat 3083.478 kb on - strand, within wcaDat 3083.489 kb on + strand, within wcaDat 3083.508 kb on - strand, within wcaDat 3083.552 kb on - strand, within wcaDat 3084.615 kb on - strand, within wcaEat 3085.170 kb on + strandat 3085.310 kb on + strand, within wcaFat 3085.337 kb on + strand, within wcaFat 3085.418 kb on + strand, within wcaF

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Per-strain Table

Position Strand Gene LocusTag Fraction K5
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3,082,504 + wcaC NOLOHH_15655 0.40 +0.7
3,082,504 + wcaC NOLOHH_15655 0.40 -2.6
3,082,564 - wcaC NOLOHH_15655 0.45 -0.6
3,082,597 + wcaC NOLOHH_15655 0.48 -0.4
3,082,640 + wcaC NOLOHH_15655 0.51 +1.0
3,082,773 - wcaC NOLOHH_15655 0.62 +0.4
3,082,849 + wcaC NOLOHH_15655 0.68 +1.4
3,082,970 + wcaC NOLOHH_15655 0.78 +1.3
3,083,133 + +0.4
3,083,208 - -0.9
3,083,256 + +1.4
3,083,323 + +1.4
3,083,412 - wcaD NOLOHH_15660 0.17 +1.7
3,083,415 + wcaD NOLOHH_15660 0.17 +1.0
3,083,448 + wcaD NOLOHH_15660 0.20 -0.3
3,083,449 - wcaD NOLOHH_15660 0.20 +0.7
3,083,476 - wcaD NOLOHH_15660 0.22 -0.4
3,083,478 - wcaD NOLOHH_15660 0.22 +0.8
3,083,489 + wcaD NOLOHH_15660 0.23 +0.4
3,083,508 - wcaD NOLOHH_15660 0.25 -0.6
3,083,552 - wcaD NOLOHH_15660 0.28 +0.0
3,084,615 - wcaE NOLOHH_15665 0.24 +1.4
3,085,170 + -1.2
3,085,310 + wcaF NOLOHH_15670 0.20 -0.6
3,085,337 + wcaF NOLOHH_15670 0.25 -0.6
3,085,418 + wcaF NOLOHH_15670 0.40 +1.4

Or see this region's nucleotide sequence