Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gcvA and ygdD are separated by 18 nucleotides ygdD and rlmM overlap by 8 nucleotides rlmM and fucR are separated by 43 nucleotides fucR and fucU are separated by 57 nucleotides
NOLOHH_12000: gcvA - glycine cleavage system transcriptional regulator GcvA, at 2,324,578 to 2,325,495
gcvA
NOLOHH_12005: ygdD - UPF0382 inner membrane protein YgdD, at 2,325,514 to 2,325,909
ygdD
NOLOHH_12010: rlmM - 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM, at 2,325,902 to 2,327,002
rlmM
NOLOHH_12015: fucR - L-fucose operon activator, at 2,327,046 to 2,327,777
fucR
NOLOHH_12020: fucU - L-fucose mutarotase, at 2,327,835 to 2,328,257
fucU
Position (kb)
2325
2326
2327
2328 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2324.925 kb on + strand, within gcvA at 2324.982 kb on + strand, within gcvA at 2325.011 kb on + strand, within gcvA at 2325.067 kb on + strand, within gcvA at 2325.152 kb on + strand, within gcvA at 2325.152 kb on + strand, within gcvA at 2325.152 kb on + strand, within gcvA at 2325.152 kb on + strand, within gcvA at 2325.152 kb on + strand, within gcvA at 2325.184 kb on + strand, within gcvA at 2325.184 kb on + strand, within gcvA at 2325.184 kb on + strand, within gcvA at 2325.184 kb on + strand, within gcvA at 2325.184 kb on + strand, within gcvA at 2325.185 kb on - strand, within gcvA at 2325.186 kb on + strand, within gcvA at 2325.186 kb on + strand, within gcvA at 2325.186 kb on + strand, within gcvA at 2325.297 kb on + strand, within gcvA at 2325.297 kb on + strand, within gcvA at 2325.348 kb on + strand, within gcvA at 2325.414 kb on + strand at 2325.493 kb on + strand at 2325.493 kb on + strand at 2325.493 kb on + strand at 2325.499 kb on - strand at 2325.528 kb on + strand at 2325.528 kb on + strand at 2325.528 kb on + strand at 2325.528 kb on + strand at 2325.596 kb on + strand, within ygdD at 2325.601 kb on - strand, within ygdD at 2325.677 kb on + strand, within ygdD at 2325.677 kb on + strand, within ygdD at 2325.721 kb on + strand, within ygdD at 2325.863 kb on + strand, within ygdD at 2325.863 kb on + strand, within ygdD at 2325.863 kb on + strand, within ygdD at 2325.896 kb on + strand at 2325.907 kb on + strand at 2325.908 kb on - strand at 2325.915 kb on + strand at 2325.915 kb on + strand at 2325.915 kb on + strand at 2325.915 kb on + strand at 2325.915 kb on + strand at 2325.915 kb on + strand at 2325.916 kb on - strand at 2325.916 kb on - strand at 2325.916 kb on - strand at 2325.916 kb on - strand at 2326.022 kb on + strand, within rlmM at 2326.032 kb on - strand, within rlmM at 2326.156 kb on + strand, within rlmM at 2326.156 kb on + strand, within rlmM at 2326.156 kb on + strand, within rlmM at 2326.157 kb on - strand, within rlmM at 2326.157 kb on - strand, within rlmM at 2326.188 kb on + strand, within rlmM at 2326.188 kb on + strand, within rlmM at 2326.191 kb on + strand, within rlmM at 2326.248 kb on + strand, within rlmM at 2326.271 kb on - strand, within rlmM at 2326.320 kb on - strand, within rlmM at 2326.320 kb on - strand, within rlmM at 2326.350 kb on + strand, within rlmM at 2326.350 kb on + strand, within rlmM at 2326.350 kb on + strand, within rlmM at 2326.350 kb on + strand, within rlmM at 2326.350 kb on + strand, within rlmM at 2326.376 kb on + strand, within rlmM at 2326.377 kb on - strand, within rlmM at 2326.391 kb on + strand, within rlmM at 2326.391 kb on + strand, within rlmM at 2326.391 kb on + strand, within rlmM at 2326.391 kb on + strand, within rlmM at 2326.403 kb on - strand, within rlmM at 2326.415 kb on + strand, within rlmM at 2326.415 kb on + strand, within rlmM at 2326.415 kb on + strand, within rlmM at 2326.415 kb on + strand, within rlmM at 2326.415 kb on + strand, within rlmM at 2326.415 kb on + strand, within rlmM at 2326.416 kb on - strand, within rlmM at 2326.416 kb on - strand, within rlmM at 2326.416 kb on - strand, within rlmM at 2326.417 kb on + strand, within rlmM at 2326.417 kb on + strand, within rlmM at 2326.418 kb on - strand, within rlmM at 2326.498 kb on + strand, within rlmM at 2326.498 kb on + strand, within rlmM at 2326.554 kb on + strand, within rlmM at 2326.554 kb on + strand, within rlmM at 2326.580 kb on + strand, within rlmM at 2326.580 kb on + strand, within rlmM at 2326.581 kb on - strand, within rlmM at 2326.581 kb on - strand, within rlmM at 2326.610 kb on + strand, within rlmM at 2326.711 kb on + strand, within rlmM at 2326.711 kb on + strand, within rlmM at 2326.712 kb on - strand, within rlmM at 2326.733 kb on - strand, within rlmM at 2326.865 kb on + strand, within rlmM at 2326.865 kb on + strand, within rlmM at 2326.865 kb on + strand, within rlmM at 2326.896 kb on + strand at 2326.896 kb on + strand at 2326.896 kb on + strand at 2327.090 kb on + strand at 2327.141 kb on + strand, within fucR at 2327.153 kb on + strand, within fucR at 2327.154 kb on - strand, within fucR at 2327.154 kb on - strand, within fucR at 2327.187 kb on + strand, within fucR at 2327.188 kb on - strand, within fucR at 2327.188 kb on - strand, within fucR at 2327.203 kb on + strand, within fucR at 2327.271 kb on - strand, within fucR at 2327.294 kb on + strand, within fucR at 2327.324 kb on - strand, within fucR at 2327.324 kb on - strand, within fucR at 2327.324 kb on - strand, within fucR at 2327.332 kb on + strand, within fucR at 2327.332 kb on + strand, within fucR at 2327.333 kb on - strand, within fucR at 2327.333 kb on - strand, within fucR at 2327.459 kb on - strand, within fucR at 2327.481 kb on + strand, within fucR at 2327.481 kb on + strand, within fucR at 2327.540 kb on + strand, within fucR at 2327.548 kb on + strand, within fucR at 2327.606 kb on + strand, within fucR at 2327.608 kb on + strand, within fucR at 2327.608 kb on + strand, within fucR at 2327.608 kb on + strand, within fucR at 2327.609 kb on - strand, within fucR at 2327.609 kb on - strand, within fucR at 2327.609 kb on - strand, within fucR at 2327.612 kb on + strand, within fucR at 2327.695 kb on - strand, within fucR at 2327.729 kb on + strand at 2327.777 kb on + strand at 2327.777 kb on + strand at 2327.781 kb on + strand at 2327.782 kb on - strand at 2327.836 kb on + strand at 2327.873 kb on - strand at 2327.973 kb on + strand, within fucU at 2327.974 kb on - strand, within fucU at 2327.988 kb on - strand, within fucU at 2327.988 kb on - strand, within fucU
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 2,324,925 + gcvA NOLOHH_12000 0.38 -1.4 2,324,982 + gcvA NOLOHH_12000 0.44 -0.4 2,325,011 + gcvA NOLOHH_12000 0.47 -1.1 2,325,067 + gcvA NOLOHH_12000 0.53 -1.9 2,325,152 + gcvA NOLOHH_12000 0.63 +0.2 2,325,152 + gcvA NOLOHH_12000 0.63 -0.7 2,325,152 + gcvA NOLOHH_12000 0.63 +0.4 2,325,152 + gcvA NOLOHH_12000 0.63 -1.2 2,325,152 + gcvA NOLOHH_12000 0.63 -0.1 2,325,184 + gcvA NOLOHH_12000 0.66 -2.8 2,325,184 + gcvA NOLOHH_12000 0.66 -1.2 2,325,184 + gcvA NOLOHH_12000 0.66 -0.8 2,325,184 + gcvA NOLOHH_12000 0.66 -0.6 2,325,184 + gcvA NOLOHH_12000 0.66 -1.7 2,325,185 - gcvA NOLOHH_12000 0.66 -0.1 2,325,186 + gcvA NOLOHH_12000 0.66 +0.7 2,325,186 + gcvA NOLOHH_12000 0.66 -0.4 2,325,186 + gcvA NOLOHH_12000 0.66 +0.6 2,325,297 + gcvA NOLOHH_12000 0.78 -1.2 2,325,297 + gcvA NOLOHH_12000 0.78 -0.2 2,325,348 + gcvA NOLOHH_12000 0.84 -0.4 2,325,414 + +0.7 2,325,493 + +0.3 2,325,493 + +1.4 2,325,493 + +0.6 2,325,499 - -0.3 2,325,528 + -0.3 2,325,528 + -0.6 2,325,528 + +0.7 2,325,528 + -0.3 2,325,596 + ygdD NOLOHH_12005 0.21 +0.3 2,325,601 - ygdD NOLOHH_12005 0.22 +0.6 2,325,677 + ygdD NOLOHH_12005 0.41 +1.1 2,325,677 + ygdD NOLOHH_12005 0.41 +0.8 2,325,721 + ygdD NOLOHH_12005 0.52 -0.7 2,325,863 + ygdD NOLOHH_12005 0.88 +1.3 2,325,863 + ygdD NOLOHH_12005 0.88 +0.7 2,325,863 + ygdD NOLOHH_12005 0.88 -0.0 2,325,896 + -0.2 2,325,907 + -1.9 2,325,908 - -0.6 2,325,915 + -0.4 2,325,915 + +0.0 2,325,915 + -0.4 2,325,915 + -0.0 2,325,915 + +0.4 2,325,915 + +0.3 2,325,916 - +1.7 2,325,916 - +0.5 2,325,916 - +0.4 2,325,916 - -1.6 2,326,022 + rlmM NOLOHH_12010 0.11 +0.4 2,326,032 - rlmM NOLOHH_12010 0.12 +1.7 2,326,156 + rlmM NOLOHH_12010 0.23 -0.2 2,326,156 + rlmM NOLOHH_12010 0.23 +0.7 2,326,156 + rlmM NOLOHH_12010 0.23 -1.5 2,326,157 - rlmM NOLOHH_12010 0.23 +0.4 2,326,157 - rlmM NOLOHH_12010 0.23 -0.3 2,326,188 + rlmM NOLOHH_12010 0.26 -0.9 2,326,188 + rlmM NOLOHH_12010 0.26 -1.9 2,326,191 + rlmM NOLOHH_12010 0.26 +1.3 2,326,248 + rlmM NOLOHH_12010 0.31 -0.5 2,326,271 - rlmM NOLOHH_12010 0.34 +0.1 2,326,320 - rlmM NOLOHH_12010 0.38 +0.4 2,326,320 - rlmM NOLOHH_12010 0.38 +1.4 2,326,350 + rlmM NOLOHH_12010 0.41 -0.6 2,326,350 + rlmM NOLOHH_12010 0.41 -1.0 2,326,350 + rlmM NOLOHH_12010 0.41 +0.5 2,326,350 + rlmM NOLOHH_12010 0.41 +0.8 2,326,350 + rlmM NOLOHH_12010 0.41 +2.0 2,326,376 + rlmM NOLOHH_12010 0.43 -0.6 2,326,377 - rlmM NOLOHH_12010 0.43 -1.5 2,326,391 + rlmM NOLOHH_12010 0.44 -1.4 2,326,391 + rlmM NOLOHH_12010 0.44 +0.6 2,326,391 + rlmM NOLOHH_12010 0.44 -1.0 2,326,391 + rlmM NOLOHH_12010 0.44 +0.0 2,326,403 - rlmM NOLOHH_12010 0.46 -0.7 2,326,415 + rlmM NOLOHH_12010 0.47 -1.6 2,326,415 + rlmM NOLOHH_12010 0.47 -1.0 2,326,415 + rlmM NOLOHH_12010 0.47 -0.3 2,326,415 + rlmM NOLOHH_12010 0.47 +0.1 2,326,415 + rlmM NOLOHH_12010 0.47 +0.7 2,326,415 + rlmM NOLOHH_12010 0.47 +0.1 2,326,416 - rlmM NOLOHH_12010 0.47 -1.3 2,326,416 - rlmM NOLOHH_12010 0.47 -0.4 2,326,416 - rlmM NOLOHH_12010 0.47 -0.8 2,326,417 + rlmM NOLOHH_12010 0.47 +1.7 2,326,417 + rlmM NOLOHH_12010 0.47 -0.9 2,326,418 - rlmM NOLOHH_12010 0.47 +0.4 2,326,498 + rlmM NOLOHH_12010 0.54 +0.3 2,326,498 + rlmM NOLOHH_12010 0.54 +0.1 2,326,554 + rlmM NOLOHH_12010 0.59 -0.3 2,326,554 + rlmM NOLOHH_12010 0.59 +0.4 2,326,580 + rlmM NOLOHH_12010 0.62 +0.7 2,326,580 + rlmM NOLOHH_12010 0.62 +0.4 2,326,581 - rlmM NOLOHH_12010 0.62 -0.8 2,326,581 - rlmM NOLOHH_12010 0.62 -0.6 2,326,610 + rlmM NOLOHH_12010 0.64 +0.4 2,326,711 + rlmM NOLOHH_12010 0.73 -1.6 2,326,711 + rlmM NOLOHH_12010 0.73 +0.0 2,326,712 - rlmM NOLOHH_12010 0.74 -0.1 2,326,733 - rlmM NOLOHH_12010 0.75 +0.1 2,326,865 + rlmM NOLOHH_12010 0.87 -1.9 2,326,865 + rlmM NOLOHH_12010 0.87 +2.2 2,326,865 + rlmM NOLOHH_12010 0.87 +0.2 2,326,896 + +0.1 2,326,896 + -0.6 2,326,896 + +0.3 2,327,090 + +0.1 2,327,141 + fucR NOLOHH_12015 0.13 -0.2 2,327,153 + fucR NOLOHH_12015 0.15 -1.3 2,327,154 - fucR NOLOHH_12015 0.15 -0.2 2,327,154 - fucR NOLOHH_12015 0.15 -0.0 2,327,187 + fucR NOLOHH_12015 0.19 +0.4 2,327,188 - fucR NOLOHH_12015 0.19 +0.0 2,327,188 - fucR NOLOHH_12015 0.19 -0.6 2,327,203 + fucR NOLOHH_12015 0.21 -1.2 2,327,271 - fucR NOLOHH_12015 0.31 +0.8 2,327,294 + fucR NOLOHH_12015 0.34 -0.0 2,327,324 - fucR NOLOHH_12015 0.38 +0.2 2,327,324 - fucR NOLOHH_12015 0.38 +0.1 2,327,324 - fucR NOLOHH_12015 0.38 -0.7 2,327,332 + fucR NOLOHH_12015 0.39 -0.1 2,327,332 + fucR NOLOHH_12015 0.39 +0.4 2,327,333 - fucR NOLOHH_12015 0.39 +0.7 2,327,333 - fucR NOLOHH_12015 0.39 +0.6 2,327,459 - fucR NOLOHH_12015 0.56 +1.3 2,327,481 + fucR NOLOHH_12015 0.59 -1.4 2,327,481 + fucR NOLOHH_12015 0.59 -0.6 2,327,540 + fucR NOLOHH_12015 0.67 -0.6 2,327,548 + fucR NOLOHH_12015 0.69 +1.0 2,327,606 + fucR NOLOHH_12015 0.77 -0.6 2,327,608 + fucR NOLOHH_12015 0.77 -1.2 2,327,608 + fucR NOLOHH_12015 0.77 +0.6 2,327,608 + fucR NOLOHH_12015 0.77 +2.0 2,327,609 - fucR NOLOHH_12015 0.77 +2.2 2,327,609 - fucR NOLOHH_12015 0.77 -0.4 2,327,609 - fucR NOLOHH_12015 0.77 +0.4 2,327,612 + fucR NOLOHH_12015 0.77 +0.1 2,327,695 - fucR NOLOHH_12015 0.89 -0.0 2,327,729 + -1.2 2,327,777 + -1.8 2,327,777 + +1.0 2,327,781 + -2.8 2,327,782 - -1.2 2,327,836 + +0.8 2,327,873 - +1.0 2,327,973 + fucU NOLOHH_12020 0.33 +0.9 2,327,974 - fucU NOLOHH_12020 0.33 -0.4 2,327,988 - fucU NOLOHH_12020 0.36 +1.4 2,327,988 - fucU NOLOHH_12020 0.36 +0.2
Or see this region's nucleotide sequence