Strain Fitness in Pseudomonas sp. DMC3 around GFF2473

Experiment: Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2471 and GFF2472 overlap by 4 nucleotidesGFF2472 and GFF2473 are separated by 155 nucleotidesGFF2473 and GFF2474 are separated by 18 nucleotidesGFF2474 and GFF2475 are separated by 53 nucleotides GFF2471 - Flagellum site-determining protein YlxH, at 259,021 to 259,851 GFF2471 GFF2472 - RNA polymerase sigma factor FliA, at 259,848 to 260,588 GFF2472 GFF2473 - Chemotaxis protein CheY, at 260,744 to 261,127 GFF2473 GFF2474 - Protein phosphatase CheZ, at 261,146 to 261,934 GFF2474 GFF2475 - hypothetical protein, at 261,988 to 264,264 GFF2475 Position (kb) 260 261 262Strain fitness (log2 ratio) -1 0 1 2at 259.847 kb on + strandat 259.847 kb on + strandat 259.848 kb on - strandat 259.848 kb on - strandat 259.872 kb on + strandat 259.899 kb on + strandat 259.900 kb on - strandat 259.900 kb on - strandat 259.944 kb on + strand, within GFF2472at 259.945 kb on - strand, within GFF2472at 259.945 kb on - strand, within GFF2472at 259.945 kb on - strand, within GFF2472at 260.034 kb on + strand, within GFF2472at 260.034 kb on + strand, within GFF2472at 260.034 kb on + strand, within GFF2472at 260.034 kb on + strand, within GFF2472at 260.068 kb on - strand, within GFF2472at 260.144 kb on + strand, within GFF2472at 260.144 kb on + strand, within GFF2472at 260.144 kb on + strand, within GFF2472at 260.144 kb on + strand, within GFF2472at 260.145 kb on - strand, within GFF2472at 260.148 kb on - strand, within GFF2472at 260.148 kb on - strand, within GFF2472at 260.186 kb on + strand, within GFF2472at 260.186 kb on + strand, within GFF2472at 260.187 kb on - strand, within GFF2472at 260.187 kb on - strand, within GFF2472at 260.187 kb on - strand, within GFF2472at 260.250 kb on + strand, within GFF2472at 260.253 kb on + strand, within GFF2472at 260.254 kb on - strand, within GFF2472at 260.254 kb on - strand, within GFF2472at 260.357 kb on + strand, within GFF2472at 260.357 kb on + strand, within GFF2472at 260.358 kb on - strand, within GFF2472at 260.358 kb on - strand, within GFF2472at 260.358 kb on - strand, within GFF2472at 260.457 kb on + strand, within GFF2472at 260.458 kb on - strand, within GFF2472at 260.460 kb on + strand, within GFF2472at 260.461 kb on - strand, within GFF2472at 260.461 kb on - strand, within GFF2472at 260.461 kb on - strand, within GFF2472at 260.461 kb on - strand, within GFF2472at 260.679 kb on + strandat 260.679 kb on + strandat 260.680 kb on - strandat 260.724 kb on - strandat 260.998 kb on + strand, within GFF2473at 261.056 kb on + strand, within GFF2473at 261.056 kb on + strand, within GFF2473at 261.056 kb on + strand, within GFF2473at 261.057 kb on - strand, within GFF2473at 261.057 kb on - strand, within GFF2473at 261.057 kb on - strand, within GFF2473at 261.145 kb on + strandat 261.146 kb on - strandat 261.146 kb on - strandat 261.146 kb on - strandat 261.146 kb on - strandat 261.155 kb on - strandat 261.416 kb on + strand, within GFF2474at 261.416 kb on + strand, within GFF2474at 261.668 kb on + strand, within GFF2474at 261.669 kb on - strand, within GFF2474at 261.869 kb on - strandat 261.933 kb on - strandat 261.964 kb on + strandat 261.972 kb on + strandat 261.973 kb on - strandat 261.987 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane
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259,847 + +0.6
259,847 + +1.0
259,848 - +0.5
259,848 - +1.0
259,872 + +0.8
259,899 + +0.4
259,900 - +0.4
259,900 - +2.1
259,944 + GFF2472 0.13 +0.5
259,945 - GFF2472 0.13 +0.6
259,945 - GFF2472 0.13 +0.4
259,945 - GFF2472 0.13 +1.2
260,034 + GFF2472 0.25 +1.2
260,034 + GFF2472 0.25 +1.0
260,034 + GFF2472 0.25 +1.3
260,034 + GFF2472 0.25 +1.2
260,068 - GFF2472 0.30 +1.3
260,144 + GFF2472 0.40 +1.0
260,144 + GFF2472 0.40 +1.6
260,144 + GFF2472 0.40 +0.4
260,144 + GFF2472 0.40 +1.0
260,145 - GFF2472 0.40 +0.7
260,148 - GFF2472 0.40 +1.2
260,148 - GFF2472 0.40 +1.8
260,186 + GFF2472 0.46 +0.4
260,186 + GFF2472 0.46 +0.7
260,187 - GFF2472 0.46 -0.2
260,187 - GFF2472 0.46 +1.1
260,187 - GFF2472 0.46 +0.7
260,250 + GFF2472 0.54 +1.3
260,253 + GFF2472 0.55 +0.1
260,254 - GFF2472 0.55 -0.2
260,254 - GFF2472 0.55 -0.8
260,357 + GFF2472 0.69 +1.0
260,357 + GFF2472 0.69 +0.8
260,358 - GFF2472 0.69 +2.3
260,358 - GFF2472 0.69 +1.7
260,358 - GFF2472 0.69 +1.0
260,457 + GFF2472 0.82 +0.3
260,458 - GFF2472 0.82 +0.9
260,460 + GFF2472 0.83 +0.9
260,461 - GFF2472 0.83 +1.1
260,461 - GFF2472 0.83 +0.8
260,461 - GFF2472 0.83 +0.6
260,461 - GFF2472 0.83 +1.6
260,679 + -0.8
260,679 + -0.1
260,680 - +0.8
260,724 - +0.9
260,998 + GFF2473 0.66 +1.2
261,056 + GFF2473 0.81 -0.2
261,056 + GFF2473 0.81 -1.2
261,056 + GFF2473 0.81 +0.1
261,057 - GFF2473 0.82 +1.0
261,057 - GFF2473 0.82 +0.7
261,057 - GFF2473 0.82 +0.2
261,145 + +0.0
261,146 - -0.3
261,146 - -0.7
261,146 - -0.9
261,146 - -0.4
261,155 - -0.6
261,416 + GFF2474 0.34 +0.2
261,416 + GFF2474 0.34 +0.9
261,668 + GFF2474 0.66 +0.2
261,669 - GFF2474 0.66 +0.2
261,869 - -0.8
261,933 - +0.1
261,964 + -0.3
261,972 + -0.1
261,973 - -0.1
261,987 - +0.8

Or see this region's nucleotide sequence