Experiment: Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1801 and GFF1802 are separated by 156 nucleotides GFF1802 and GFF1803 are separated by 84 nucleotides GFF1803 and GFF1804 overlap by 4 nucleotides
GFF1801 - Galactarate dehydratase (L-threo-forming), at 11,341 to 12,894
GFF1801
GFF1802 - hypothetical protein, at 13,051 to 13,992
GFF1802
GFF1803 - hypothetical protein, at 14,077 to 14,454
GFF1803
GFF1804 - hypothetical protein, at 14,451 to 15,617
GFF1804
Position (kb) 13
14 Strain fitness (log2 ratio) -2
-1
0
1 at 12.177 kb on + strand, within GFF1801 at 12.236 kb on - strand, within GFF1801 at 12.310 kb on + strand, within GFF1801 at 12.310 kb on + strand, within GFF1801 at 12.310 kb on + strand, within GFF1801 at 12.311 kb on - strand, within GFF1801 at 12.311 kb on - strand, within GFF1801 at 12.311 kb on - strand, within GFF1801 at 12.311 kb on - strand, within GFF1801 at 12.377 kb on - strand, within GFF1801 at 12.377 kb on - strand, within GFF1801 at 12.684 kb on - strand, within GFF1801 at 12.796 kb on + strand at 12.797 kb on - strand at 12.799 kb on + strand at 12.800 kb on - strand at 12.800 kb on - strand at 12.800 kb on - strand at 12.939 kb on + strand at 12.939 kb on + strand at 12.939 kb on + strand at 13.009 kb on + strand at 13.264 kb on + strand, within GFF1802 at 13.265 kb on - strand, within GFF1802 at 13.348 kb on + strand, within GFF1802 at 13.349 kb on - strand, within GFF1802 at 13.385 kb on + strand, within GFF1802 at 13.386 kb on - strand, within GFF1802 at 13.411 kb on + strand, within GFF1802 at 13.412 kb on - strand, within GFF1802 at 13.420 kb on + strand, within GFF1802 at 13.468 kb on + strand, within GFF1802 at 13.468 kb on + strand, within GFF1802 at 13.469 kb on - strand, within GFF1802 at 13.469 kb on - strand, within GFF1802 at 13.469 kb on - strand, within GFF1802 at 13.469 kb on - strand, within GFF1802 at 13.501 kb on + strand, within GFF1802 at 13.598 kb on - strand, within GFF1802 at 13.598 kb on - strand, within GFF1802 at 13.709 kb on + strand, within GFF1802 at 13.709 kb on + strand, within GFF1802 at 13.945 kb on - strand at 13.991 kb on - strand at 13.991 kb on - strand at 14.029 kb on + strand at 14.029 kb on + strand at 14.029 kb on + strand at 14.029 kb on + strand at 14.029 kb on + strand at 14.052 kb on - strand at 14.052 kb on - strand at 14.150 kb on + strand, within GFF1803 at 14.150 kb on + strand, within GFF1803 at 14.150 kb on + strand, within GFF1803 at 14.151 kb on - strand, within GFF1803 at 14.536 kb on + strand at 14.536 kb on + strand at 14.536 kb on + strand at 14.536 kb on + strand at 14.536 kb on + strand at 14.537 kb on - strand at 14.537 kb on - strand at 14.537 kb on - strand at 14.668 kb on + strand, within GFF1804 at 14.669 kb on - strand, within GFF1804
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane remove 12,177 + GFF1801 0.54 -0.5 12,236 - GFF1801 0.58 +0.4 12,310 + GFF1801 0.62 +0.7 12,310 + GFF1801 0.62 -0.9 12,310 + GFF1801 0.62 -0.5 12,311 - GFF1801 0.62 -0.3 12,311 - GFF1801 0.62 -0.1 12,311 - GFF1801 0.62 +0.2 12,311 - GFF1801 0.62 +0.3 12,377 - GFF1801 0.67 -0.3 12,377 - GFF1801 0.67 -0.5 12,684 - GFF1801 0.86 +0.3 12,796 + -1.0 12,797 - -0.7 12,799 + -0.2 12,800 - +1.0 12,800 - +0.2 12,800 - -0.6 12,939 + -0.7 12,939 + -0.2 12,939 + +0.4 13,009 + -2.0 13,264 + GFF1802 0.23 -0.0 13,265 - GFF1802 0.23 +1.3 13,348 + GFF1802 0.32 -0.7 13,349 - GFF1802 0.32 -1.0 13,385 + GFF1802 0.35 +0.2 13,386 - GFF1802 0.36 -0.1 13,411 + GFF1802 0.38 -0.0 13,412 - GFF1802 0.38 -0.8 13,420 + GFF1802 0.39 +0.4 13,468 + GFF1802 0.44 +0.6 13,468 + GFF1802 0.44 -1.9 13,469 - GFF1802 0.44 -0.5 13,469 - GFF1802 0.44 -0.1 13,469 - GFF1802 0.44 -0.4 13,469 - GFF1802 0.44 +0.3 13,501 + GFF1802 0.48 -0.0 13,598 - GFF1802 0.58 -1.9 13,598 - GFF1802 0.58 +0.2 13,709 + GFF1802 0.70 -0.3 13,709 + GFF1802 0.70 +0.2 13,945 - -0.1 13,991 - -0.5 13,991 - -1.6 14,029 + +1.4 14,029 + +0.7 14,029 + +1.0 14,029 + -0.0 14,029 + +0.2 14,052 - +0.4 14,052 - -0.6 14,150 + GFF1803 0.19 -0.8 14,150 + GFF1803 0.19 -0.1 14,150 + GFF1803 0.19 -0.1 14,151 - GFF1803 0.20 +0.4 14,536 + -0.6 14,536 + +0.3 14,536 + +0.1 14,536 + +0.6 14,536 + +0.2 14,537 - -1.0 14,537 - -0.3 14,537 - +0.2 14,668 + GFF1804 0.19 +0.1 14,669 - GFF1804 0.19 +0.9
Or see this region's nucleotide sequence