Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NOLOHH_16395 and fliA are separated by 164 nucleotides fliA and fliZ are separated by 45 nucleotides fliZ and tcyJ are separated by 30 nucleotides tcyJ and dcyD are separated by 104 nucleotides
NOLOHH_16395: NOLOHH_16395 - flagellin FliC, at 3,227,872 to 3,229,347
_16395
NOLOHH_16400: fliA - RNA polymerase sigma factor FliA, at 3,229,512 to 3,230,231
fliA
NOLOHH_16405: fliZ - flagella biosynthesis regulatory protein FliZ, at 3,230,277 to 3,230,828
fliZ
NOLOHH_16410: tcyJ - cystine ABC transporter substrate-binding protein, at 3,230,859 to 3,231,716
tcyJ
NOLOHH_16415: dcyD - D-cysteine desulfhydrase, at 3,231,821 to 3,232,807
dcyD
Position (kb)
3230
3231 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3229.328 kb on + strand at 3229.329 kb on - strand at 3229.329 kb on - strand at 3229.351 kb on - strand at 3229.357 kb on - strand at 3229.491 kb on + strand at 3229.493 kb on + strand at 3229.524 kb on + strand at 3229.525 kb on - strand at 3229.540 kb on + strand at 3229.540 kb on + strand at 3229.569 kb on + strand at 3229.569 kb on + strand at 3229.570 kb on - strand at 3229.570 kb on - strand at 3229.646 kb on - strand, within fliA at 3229.670 kb on + strand, within fliA at 3229.670 kb on + strand, within fliA at 3229.671 kb on - strand, within fliA at 3229.671 kb on - strand, within fliA at 3229.713 kb on - strand, within fliA at 3229.719 kb on + strand, within fliA at 3229.720 kb on - strand, within fliA at 3230.040 kb on - strand, within fliA at 3230.098 kb on + strand, within fliA at 3230.109 kb on + strand, within fliA at 3230.110 kb on - strand, within fliA at 3230.112 kb on + strand, within fliA at 3230.230 kb on - strand at 3230.252 kb on + strand at 3230.364 kb on - strand, within fliZ at 3230.591 kb on + strand, within fliZ at 3230.752 kb on - strand, within fliZ at 3230.819 kb on + strand at 3230.819 kb on + strand at 3230.820 kb on - strand at 3230.822 kb on - strand at 3230.823 kb on + strand at 3230.896 kb on + strand at 3230.923 kb on + strand at 3231.188 kb on + strand, within tcyJ at 3231.209 kb on + strand, within tcyJ at 3231.210 kb on - strand, within tcyJ at 3231.215 kb on + strand, within tcyJ at 3231.393 kb on + strand, within tcyJ at 3231.393 kb on + strand, within tcyJ at 3231.393 kb on + strand, within tcyJ at 3231.393 kb on + strand, within tcyJ at 3231.437 kb on + strand, within tcyJ at 3231.437 kb on + strand, within tcyJ at 3231.437 kb on + strand, within tcyJ at 3231.441 kb on + strand, within tcyJ at 3231.441 kb on + strand, within tcyJ at 3231.442 kb on - strand, within tcyJ at 3231.545 kb on + strand, within tcyJ at 3231.602 kb on + strand, within tcyJ at 3231.723 kb on + strand at 3231.723 kb on + strand at 3231.723 kb on + strand at 3231.783 kb on + strand at 3231.786 kb on + strand at 3231.786 kb on + strand at 3231.818 kb on - strand at 3231.820 kb on + strand at 3231.821 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 3,229,328 + +1.3 3,229,329 - +1.6 3,229,329 - -0.4 3,229,351 - -0.4 3,229,357 - +1.0 3,229,491 + -1.0 3,229,493 + -1.0 3,229,524 + +1.6 3,229,525 - -0.4 3,229,540 + -2.6 3,229,540 + +0.6 3,229,569 + -0.0 3,229,569 + +0.2 3,229,570 - +0.6 3,229,570 - +0.3 3,229,646 - fliA NOLOHH_16400 0.19 -1.3 3,229,670 + fliA NOLOHH_16400 0.22 -2.0 3,229,670 + fliA NOLOHH_16400 0.22 +2.2 3,229,671 - fliA NOLOHH_16400 0.22 -0.4 3,229,671 - fliA NOLOHH_16400 0.22 -0.4 3,229,713 - fliA NOLOHH_16400 0.28 +0.6 3,229,719 + fliA NOLOHH_16400 0.29 +0.3 3,229,720 - fliA NOLOHH_16400 0.29 -2.7 3,230,040 - fliA NOLOHH_16400 0.73 -2.4 3,230,098 + fliA NOLOHH_16400 0.81 +0.8 3,230,109 + fliA NOLOHH_16400 0.83 +0.6 3,230,110 - fliA NOLOHH_16400 0.83 +1.0 3,230,112 + fliA NOLOHH_16400 0.83 +0.9 3,230,230 - +0.6 3,230,252 + +0.4 3,230,364 - fliZ NOLOHH_16405 0.16 +0.6 3,230,591 + fliZ NOLOHH_16405 0.57 +1.9 3,230,752 - fliZ NOLOHH_16405 0.86 +1.6 3,230,819 + +1.9 3,230,819 + +0.6 3,230,820 - +0.1 3,230,822 - -0.0 3,230,823 + +0.6 3,230,896 + -0.4 3,230,923 + +1.0 3,231,188 + tcyJ NOLOHH_16410 0.38 +0.2 3,231,209 + tcyJ NOLOHH_16410 0.41 +0.6 3,231,210 - tcyJ NOLOHH_16410 0.41 +1.6 3,231,215 + tcyJ NOLOHH_16410 0.41 -1.0 3,231,393 + tcyJ NOLOHH_16410 0.62 +1.6 3,231,393 + tcyJ NOLOHH_16410 0.62 +0.2 3,231,393 + tcyJ NOLOHH_16410 0.62 -1.5 3,231,393 + tcyJ NOLOHH_16410 0.62 +1.1 3,231,437 + tcyJ NOLOHH_16410 0.67 +0.6 3,231,437 + tcyJ NOLOHH_16410 0.67 -0.4 3,231,437 + tcyJ NOLOHH_16410 0.67 -0.1 3,231,441 + tcyJ NOLOHH_16410 0.68 -0.2 3,231,441 + tcyJ NOLOHH_16410 0.68 -1.2 3,231,442 - tcyJ NOLOHH_16410 0.68 -0.4 3,231,545 + tcyJ NOLOHH_16410 0.80 +0.9 3,231,602 + tcyJ NOLOHH_16410 0.87 +2.2 3,231,723 + -0.4 3,231,723 + -1.4 3,231,723 + -0.7 3,231,783 + +0.6 3,231,786 + +2.2 3,231,786 + -0.4 3,231,818 - +0.0 3,231,820 + -0.4 3,231,821 - +0.9
Or see this region's nucleotide sequence