Strain Fitness in Escherichia coli ECOR27 around NOLOHH_15675

Experiment: K5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaE and wcaF are separated by 15 nucleotideswcaF and NOLOHH_15675 are separated by 25 nucleotidesNOLOHH_15675 and fcl are separated by 2 nucleotidesfcl and gmm overlap by 4 nucleotides NOLOHH_15665: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 3,084,436 to 3,085,182 wcaE NOLOHH_15670: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 3,085,198 to 3,085,746 wcaF NOLOHH_15675: NOLOHH_15675 - GDP-mannose 4,6-dehydratase, at 3,085,772 to 3,086,893 _15675 NOLOHH_15680: fcl - GDP-L-fucose synthase, at 3,086,896 to 3,087,864 fcl NOLOHH_15685: gmm - GDP-mannose mannosyl hydrolase, at 3,087,861 to 3,088,343 gmm Position (kb) 3085 3086 3087Strain fitness (log2 ratio) -2 -1 0 1 2at 3085.170 kb on + strandat 3085.310 kb on + strand, within wcaFat 3085.337 kb on + strand, within wcaFat 3085.418 kb on + strand, within wcaFat 3085.569 kb on + strand, within wcaFat 3085.864 kb on + strandat 3086.021 kb on + strand, within NOLOHH_15675at 3086.021 kb on + strand, within NOLOHH_15675at 3086.181 kb on - strand, within NOLOHH_15675at 3086.304 kb on - strand, within NOLOHH_15675at 3086.304 kb on - strand, within NOLOHH_15675at 3086.304 kb on - strand, within NOLOHH_15675at 3086.421 kb on - strand, within NOLOHH_15675at 3086.469 kb on - strand, within NOLOHH_15675at 3086.473 kb on - strand, within NOLOHH_15675at 3086.702 kb on + strand, within NOLOHH_15675at 3086.891 kb on + strandat 3086.902 kb on - strandat 3086.991 kb on - strandat 3087.074 kb on - strand, within fclat 3087.222 kb on + strand, within fclat 3087.222 kb on + strand, within fclat 3087.223 kb on - strand, within fclat 3087.223 kb on - strand, within fclat 3087.223 kb on - strand, within fclat 3087.227 kb on - strand, within fclat 3087.267 kb on - strand, within fclat 3087.343 kb on + strand, within fclat 3087.343 kb on + strand, within fclat 3087.511 kb on - strand, within fclat 3087.692 kb on - strand, within fclat 3087.781 kb on + strandat 3087.820 kb on + strandat 3087.820 kb on + strandat 3087.821 kb on - strandat 3087.821 kb on - strandat 3087.821 kb on - strandat 3087.821 kb on - strandat 3087.871 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K5
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3,085,170 + -0.3
3,085,310 + wcaF NOLOHH_15670 0.20 -0.4
3,085,337 + wcaF NOLOHH_15670 0.25 +0.8
3,085,418 + wcaF NOLOHH_15670 0.40 +2.2
3,085,569 + wcaF NOLOHH_15670 0.68 -2.4
3,085,864 + -1.7
3,086,021 + NOLOHH_15675 0.22 -0.4
3,086,021 + NOLOHH_15675 0.22 -0.4
3,086,181 - NOLOHH_15675 0.36 -1.0
3,086,304 - NOLOHH_15675 0.47 -1.0
3,086,304 - NOLOHH_15675 0.47 +0.6
3,086,304 - NOLOHH_15675 0.47 -1.0
3,086,421 - NOLOHH_15675 0.58 +0.8
3,086,469 - NOLOHH_15675 0.62 +1.6
3,086,473 - NOLOHH_15675 0.62 +1.0
3,086,702 + NOLOHH_15675 0.83 +0.6
3,086,891 + +0.1
3,086,902 - +0.6
3,086,991 - -0.0
3,087,074 - fcl NOLOHH_15680 0.18 -0.7
3,087,222 + fcl NOLOHH_15680 0.34 +2.2
3,087,222 + fcl NOLOHH_15680 0.34 -0.4
3,087,223 - fcl NOLOHH_15680 0.34 +0.8
3,087,223 - fcl NOLOHH_15680 0.34 +0.6
3,087,223 - fcl NOLOHH_15680 0.34 -0.2
3,087,227 - fcl NOLOHH_15680 0.34 -0.2
3,087,267 - fcl NOLOHH_15680 0.38 +1.6
3,087,343 + fcl NOLOHH_15680 0.46 +0.6
3,087,343 + fcl NOLOHH_15680 0.46 -2.6
3,087,511 - fcl NOLOHH_15680 0.63 -1.6
3,087,692 - fcl NOLOHH_15680 0.82 -0.4
3,087,781 + +0.2
3,087,820 + -0.4
3,087,820 + +0.2
3,087,821 - -0.4
3,087,821 - +1.6
3,087,821 - -0.0
3,087,821 - -1.0
3,087,871 + +0.2

Or see this region's nucleotide sequence