Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaE and wcaF are separated by 15 nucleotides wcaF and NOLOHH_15675 are separated by 25 nucleotides NOLOHH_15675 and fcl are separated by 2 nucleotides fcl and gmm overlap by 4 nucleotides
NOLOHH_15665: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 3,084,436 to 3,085,182
wcaE
NOLOHH_15670: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 3,085,198 to 3,085,746
wcaF
NOLOHH_15675: NOLOHH_15675 - GDP-mannose 4,6-dehydratase, at 3,085,772 to 3,086,893
_15675
NOLOHH_15680: fcl - GDP-L-fucose synthase, at 3,086,896 to 3,087,864
fcl
NOLOHH_15685: gmm - GDP-mannose mannosyl hydrolase, at 3,087,861 to 3,088,343
gmm
Position (kb)
3085
3086
3087 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3085.170 kb on + strand at 3085.310 kb on + strand, within wcaF at 3085.337 kb on + strand, within wcaF at 3085.418 kb on + strand, within wcaF at 3085.569 kb on + strand, within wcaF at 3085.864 kb on + strand at 3086.021 kb on + strand, within NOLOHH_15675 at 3086.021 kb on + strand, within NOLOHH_15675 at 3086.181 kb on - strand, within NOLOHH_15675 at 3086.304 kb on - strand, within NOLOHH_15675 at 3086.304 kb on - strand, within NOLOHH_15675 at 3086.304 kb on - strand, within NOLOHH_15675 at 3086.421 kb on - strand, within NOLOHH_15675 at 3086.469 kb on - strand, within NOLOHH_15675 at 3086.473 kb on - strand, within NOLOHH_15675 at 3086.702 kb on + strand, within NOLOHH_15675 at 3086.891 kb on + strand at 3086.902 kb on - strand at 3086.991 kb on - strand at 3087.074 kb on - strand, within fcl at 3087.222 kb on + strand, within fcl at 3087.222 kb on + strand, within fcl at 3087.223 kb on - strand, within fcl at 3087.223 kb on - strand, within fcl at 3087.223 kb on - strand, within fcl at 3087.227 kb on - strand, within fcl at 3087.267 kb on - strand, within fcl at 3087.343 kb on + strand, within fcl at 3087.343 kb on + strand, within fcl at 3087.511 kb on - strand, within fcl at 3087.692 kb on - strand, within fcl at 3087.781 kb on + strand at 3087.820 kb on + strand at 3087.820 kb on + strand at 3087.821 kb on - strand at 3087.821 kb on - strand at 3087.821 kb on - strand at 3087.821 kb on - strand at 3087.871 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 3,085,170 + -0.3 3,085,310 + wcaF NOLOHH_15670 0.20 -0.4 3,085,337 + wcaF NOLOHH_15670 0.25 +0.8 3,085,418 + wcaF NOLOHH_15670 0.40 +2.2 3,085,569 + wcaF NOLOHH_15670 0.68 -2.4 3,085,864 + -1.7 3,086,021 + NOLOHH_15675 0.22 -0.4 3,086,021 + NOLOHH_15675 0.22 -0.4 3,086,181 - NOLOHH_15675 0.36 -1.0 3,086,304 - NOLOHH_15675 0.47 -1.0 3,086,304 - NOLOHH_15675 0.47 +0.6 3,086,304 - NOLOHH_15675 0.47 -1.0 3,086,421 - NOLOHH_15675 0.58 +0.8 3,086,469 - NOLOHH_15675 0.62 +1.6 3,086,473 - NOLOHH_15675 0.62 +1.0 3,086,702 + NOLOHH_15675 0.83 +0.6 3,086,891 + +0.1 3,086,902 - +0.6 3,086,991 - -0.0 3,087,074 - fcl NOLOHH_15680 0.18 -0.7 3,087,222 + fcl NOLOHH_15680 0.34 +2.2 3,087,222 + fcl NOLOHH_15680 0.34 -0.4 3,087,223 - fcl NOLOHH_15680 0.34 +0.8 3,087,223 - fcl NOLOHH_15680 0.34 +0.6 3,087,223 - fcl NOLOHH_15680 0.34 -0.2 3,087,227 - fcl NOLOHH_15680 0.34 -0.2 3,087,267 - fcl NOLOHH_15680 0.38 +1.6 3,087,343 + fcl NOLOHH_15680 0.46 +0.6 3,087,343 + fcl NOLOHH_15680 0.46 -2.6 3,087,511 - fcl NOLOHH_15680 0.63 -1.6 3,087,692 - fcl NOLOHH_15680 0.82 -0.4 3,087,781 + +0.2 3,087,820 + -0.4 3,087,820 + +0.2 3,087,821 - -0.4 3,087,821 - +1.6 3,087,821 - -0.0 3,087,821 - -1.0 3,087,871 + +0.2
Or see this region's nucleotide sequence