Strain Fitness in Escherichia coli ECOR27 around NOLOHH_14360

Experiment: K5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisJ and hisQ are separated by 89 nucleotideshisQ and hisM overlap by 4 nucleotideshisM and hisP are separated by 7 nucleotideshisP and rpnB are separated by 196 nucleotides NOLOHH_14350: hisJ - histidine ABC transporter substrate-binding protein HisJ, at 2,795,204 to 2,795,986 hisJ NOLOHH_14355: hisQ - histidine ABC transporter permease HisQ, at 2,796,076 to 2,796,762 hisQ NOLOHH_14360: hisM - Histidine transport system permease protein HisM, at 2,796,759 to 2,797,475 hisM NOLOHH_14365: hisP - histidine ABC transporter ATP-binding protein HisP, at 2,797,483 to 2,798,256 hisP NOLOHH_14370: rpnB - recombination-promoting nuclease RpnB, at 2,798,453 to 2,799,355 rpnB Position (kb) 2796 2797 2798Strain fitness (log2 ratio) -2 -1 0 1 2at 2795.824 kb on + strand, within hisJat 2795.936 kb on + strandat 2795.946 kb on + strandat 2796.046 kb on + strandat 2796.047 kb on - strandat 2796.158 kb on + strand, within hisQat 2796.229 kb on + strand, within hisQat 2796.343 kb on - strand, within hisQat 2796.366 kb on + strand, within hisQat 2796.366 kb on + strand, within hisQat 2796.385 kb on + strand, within hisQat 2796.394 kb on + strand, within hisQat 2796.394 kb on + strand, within hisQat 2796.444 kb on + strand, within hisQat 2796.446 kb on + strand, within hisQat 2796.446 kb on + strand, within hisQat 2796.446 kb on + strand, within hisQat 2796.446 kb on + strand, within hisQat 2796.446 kb on + strand, within hisQat 2796.446 kb on + strand, within hisQat 2796.447 kb on - strand, within hisQat 2796.676 kb on + strand, within hisQat 2796.677 kb on - strand, within hisQat 2796.849 kb on - strand, within hisMat 2796.944 kb on + strand, within hisMat 2796.951 kb on - strand, within hisMat 2796.972 kb on + strand, within hisMat 2796.993 kb on + strand, within hisMat 2797.005 kb on + strand, within hisMat 2797.005 kb on + strand, within hisMat 2797.105 kb on - strand, within hisMat 2797.278 kb on - strand, within hisMat 2797.332 kb on - strand, within hisMat 2797.390 kb on + strand, within hisMat 2797.391 kb on - strand, within hisMat 2797.401 kb on + strand, within hisMat 2797.401 kb on + strand, within hisMat 2797.401 kb on + strand, within hisMat 2797.402 kb on - strand, within hisMat 2797.402 kb on - strand, within hisMat 2797.479 kb on + strandat 2797.479 kb on + strandat 2797.583 kb on + strand, within hisPat 2797.635 kb on + strand, within hisPat 2797.800 kb on + strand, within hisPat 2798.019 kb on + strand, within hisPat 2798.019 kb on + strand, within hisPat 2798.020 kb on - strand, within hisPat 2798.061 kb on + strand, within hisPat 2798.064 kb on + strand, within hisPat 2798.171 kb on + strand, within hisPat 2798.429 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K5
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2,795,824 + hisJ NOLOHH_14350 0.79 +1.4
2,795,936 + -1.4
2,795,946 + -1.0
2,796,046 + -1.2
2,796,047 - -1.0
2,796,158 + hisQ NOLOHH_14355 0.12 +1.6
2,796,229 + hisQ NOLOHH_14355 0.22 +0.6
2,796,343 - hisQ NOLOHH_14355 0.39 +2.2
2,796,366 + hisQ NOLOHH_14355 0.42 +1.6
2,796,366 + hisQ NOLOHH_14355 0.42 -2.4
2,796,385 + hisQ NOLOHH_14355 0.45 +1.0
2,796,394 + hisQ NOLOHH_14355 0.46 +1.0
2,796,394 + hisQ NOLOHH_14355 0.46 +1.0
2,796,444 + hisQ NOLOHH_14355 0.54 +0.2
2,796,446 + hisQ NOLOHH_14355 0.54 +1.1
2,796,446 + hisQ NOLOHH_14355 0.54 -1.0
2,796,446 + hisQ NOLOHH_14355 0.54 -0.7
2,796,446 + hisQ NOLOHH_14355 0.54 -0.0
2,796,446 + hisQ NOLOHH_14355 0.54 -0.4
2,796,446 + hisQ NOLOHH_14355 0.54 +1.2
2,796,447 - hisQ NOLOHH_14355 0.54 +2.2
2,796,676 + hisQ NOLOHH_14355 0.87 +0.6
2,796,677 - hisQ NOLOHH_14355 0.87 -1.0
2,796,849 - hisM NOLOHH_14360 0.13 +1.4
2,796,944 + hisM NOLOHH_14360 0.26 -0.4
2,796,951 - hisM NOLOHH_14360 0.27 -2.0
2,796,972 + hisM NOLOHH_14360 0.30 +2.2
2,796,993 + hisM NOLOHH_14360 0.33 +1.2
2,797,005 + hisM NOLOHH_14360 0.34 -1.7
2,797,005 + hisM NOLOHH_14360 0.34 +1.0
2,797,105 - hisM NOLOHH_14360 0.48 -2.0
2,797,278 - hisM NOLOHH_14360 0.72 -0.8
2,797,332 - hisM NOLOHH_14360 0.80 +0.2
2,797,390 + hisM NOLOHH_14360 0.88 -0.9
2,797,391 - hisM NOLOHH_14360 0.88 +2.2
2,797,401 + hisM NOLOHH_14360 0.90 -1.0
2,797,401 + hisM NOLOHH_14360 0.90 -0.4
2,797,401 + hisM NOLOHH_14360 0.90 +0.6
2,797,402 - hisM NOLOHH_14360 0.90 -2.2
2,797,402 - hisM NOLOHH_14360 0.90 -1.0
2,797,479 + -1.0
2,797,479 + +0.0
2,797,583 + hisP NOLOHH_14365 0.13 -0.2
2,797,635 + hisP NOLOHH_14365 0.20 +0.4
2,797,800 + hisP NOLOHH_14365 0.41 +0.6
2,798,019 + hisP NOLOHH_14365 0.69 +0.6
2,798,019 + hisP NOLOHH_14365 0.69 -0.4
2,798,020 - hisP NOLOHH_14365 0.69 +0.0
2,798,061 + hisP NOLOHH_14365 0.75 +0.4
2,798,064 + hisP NOLOHH_14365 0.75 +0.2
2,798,171 + hisP NOLOHH_14365 0.89 -1.4
2,798,429 - +0.6

Or see this region's nucleotide sequence