Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hpaD and hpaF are separated by 9 nucleotides hpaF and hpaH are separated by 67 nucleotides hpaH and hpaI are separated by 10 nucleotides hpaI and hpaX are separated by 174 nucleotides
NOLOHH_03725: hpaD - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 663,081 to 663,932
hpaD
NOLOHH_03730: hpaF - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 663,942 to 664,322
hpaF
NOLOHH_03735: hpaH - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 664,390 to 665,193
hpaH
NOLOHH_03740: hpaI - 4-hydroxy-2-oxoheptanedioate aldolase, at 665,204 to 665,992
hpaI
NOLOHH_03745: hpaX - 4-hydroxyphenylacetate permease, at 666,167 to 667,543
hpaX
Position (kb) 664
665
666 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 663.591 kb on + strand, within hpaD at 663.591 kb on + strand, within hpaD at 663.641 kb on + strand, within hpaD at 663.642 kb on - strand, within hpaD at 663.678 kb on + strand, within hpaD at 663.679 kb on - strand, within hpaD at 663.758 kb on + strand, within hpaD at 663.758 kb on + strand, within hpaD at 663.758 kb on + strand, within hpaD at 663.759 kb on - strand, within hpaD at 663.759 kb on - strand, within hpaD at 663.759 kb on - strand, within hpaD at 663.772 kb on - strand, within hpaD at 663.781 kb on - strand, within hpaD at 663.781 kb on - strand, within hpaD at 663.786 kb on + strand, within hpaD at 663.800 kb on + strand, within hpaD at 663.801 kb on - strand, within hpaD at 663.846 kb on + strand, within hpaD at 663.846 kb on + strand, within hpaD at 663.847 kb on - strand at 663.847 kb on - strand at 663.847 kb on - strand at 663.847 kb on - strand at 663.847 kb on - strand at 663.867 kb on - strand at 663.965 kb on + strand at 663.966 kb on - strand at 663.975 kb on - strand at 663.994 kb on + strand, within hpaF at 664.016 kb on + strand, within hpaF at 664.016 kb on + strand, within hpaF at 664.060 kb on + strand, within hpaF at 664.060 kb on + strand, within hpaF at 664.060 kb on + strand, within hpaF at 664.060 kb on + strand, within hpaF at 664.061 kb on - strand, within hpaF at 664.061 kb on - strand, within hpaF at 664.061 kb on - strand, within hpaF at 664.064 kb on + strand, within hpaF at 664.064 kb on + strand, within hpaF at 664.086 kb on - strand, within hpaF at 664.117 kb on - strand, within hpaF at 664.130 kb on + strand, within hpaF at 664.130 kb on + strand, within hpaF at 664.130 kb on + strand, within hpaF at 664.131 kb on - strand, within hpaF at 664.316 kb on + strand at 664.389 kb on + strand at 664.389 kb on + strand at 664.390 kb on - strand at 664.390 kb on - strand at 664.671 kb on + strand, within hpaH at 664.772 kb on - strand, within hpaH at 664.786 kb on + strand, within hpaH at 664.786 kb on + strand, within hpaH at 664.786 kb on + strand, within hpaH at 664.786 kb on + strand, within hpaH at 664.787 kb on - strand, within hpaH at 664.923 kb on + strand, within hpaH at 664.924 kb on - strand, within hpaH at 664.924 kb on - strand, within hpaH at 664.943 kb on + strand, within hpaH at 664.943 kb on + strand, within hpaH at 664.944 kb on - strand, within hpaH at 664.944 kb on - strand, within hpaH at 664.944 kb on - strand, within hpaH at 664.944 kb on - strand, within hpaH at 664.967 kb on - strand, within hpaH at 665.062 kb on + strand, within hpaH at 665.062 kb on + strand, within hpaH at 665.063 kb on - strand, within hpaH at 665.063 kb on - strand, within hpaH at 665.069 kb on + strand, within hpaH at 665.069 kb on + strand, within hpaH at 665.069 kb on + strand, within hpaH at 665.069 kb on + strand, within hpaH at 665.069 kb on + strand, within hpaH at 665.070 kb on - strand, within hpaH at 665.070 kb on - strand, within hpaH at 665.070 kb on - strand, within hpaH at 665.070 kb on - strand, within hpaH at 665.070 kb on - strand, within hpaH at 665.175 kb on + strand at 665.279 kb on + strand at 665.280 kb on - strand at 665.410 kb on - strand, within hpaI at 665.410 kb on - strand, within hpaI at 665.747 kb on + strand, within hpaI at 665.776 kb on - strand, within hpaI at 665.868 kb on - strand, within hpaI at 665.987 kb on + strand at 665.987 kb on + strand at 665.988 kb on - strand at 665.988 kb on - strand at 665.988 kb on - strand at 665.990 kb on + strand at 665.991 kb on - strand at 666.011 kb on + strand at 666.012 kb on - strand at 666.020 kb on + strand at 666.020 kb on + strand at 666.020 kb on + strand at 666.124 kb on - strand at 666.124 kb on - strand at 666.124 kb on - strand at 666.136 kb on - strand at 666.141 kb on - strand at 666.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 663,591 + hpaD NOLOHH_03725 0.60 +0.8 663,591 + hpaD NOLOHH_03725 0.60 -0.7 663,641 + hpaD NOLOHH_03725 0.66 +0.5 663,642 - hpaD NOLOHH_03725 0.66 -0.4 663,678 + hpaD NOLOHH_03725 0.70 -0.5 663,679 - hpaD NOLOHH_03725 0.70 +0.9 663,758 + hpaD NOLOHH_03725 0.79 -0.6 663,758 + hpaD NOLOHH_03725 0.79 -1.5 663,758 + hpaD NOLOHH_03725 0.79 +0.1 663,759 - hpaD NOLOHH_03725 0.80 +0.2 663,759 - hpaD NOLOHH_03725 0.80 -0.8 663,759 - hpaD NOLOHH_03725 0.80 -0.9 663,772 - hpaD NOLOHH_03725 0.81 -0.6 663,781 - hpaD NOLOHH_03725 0.82 -0.4 663,781 - hpaD NOLOHH_03725 0.82 -1.3 663,786 + hpaD NOLOHH_03725 0.83 +0.8 663,800 + hpaD NOLOHH_03725 0.84 +0.6 663,801 - hpaD NOLOHH_03725 0.85 +2.5 663,846 + hpaD NOLOHH_03725 0.90 -0.5 663,846 + hpaD NOLOHH_03725 0.90 -0.2 663,847 - -0.3 663,847 - -0.2 663,847 - -0.8 663,847 - +0.6 663,847 - +1.0 663,867 - -0.1 663,965 + -0.6 663,966 - -1.0 663,975 - +0.1 663,994 + hpaF NOLOHH_03730 0.14 -0.7 664,016 + hpaF NOLOHH_03730 0.19 -0.9 664,016 + hpaF NOLOHH_03730 0.19 +0.5 664,060 + hpaF NOLOHH_03730 0.31 +1.5 664,060 + hpaF NOLOHH_03730 0.31 +1.0 664,060 + hpaF NOLOHH_03730 0.31 -0.2 664,060 + hpaF NOLOHH_03730 0.31 +0.9 664,061 - hpaF NOLOHH_03730 0.31 -0.2 664,061 - hpaF NOLOHH_03730 0.31 +0.1 664,061 - hpaF NOLOHH_03730 0.31 -0.8 664,064 + hpaF NOLOHH_03730 0.32 +0.0 664,064 + hpaF NOLOHH_03730 0.32 +0.0 664,086 - hpaF NOLOHH_03730 0.38 -3.1 664,117 - hpaF NOLOHH_03730 0.46 -0.0 664,130 + hpaF NOLOHH_03730 0.49 +0.2 664,130 + hpaF NOLOHH_03730 0.49 +0.3 664,130 + hpaF NOLOHH_03730 0.49 +1.4 664,131 - hpaF NOLOHH_03730 0.50 -1.4 664,316 + -0.1 664,389 + -0.7 664,389 + -0.2 664,390 - -0.4 664,390 - +0.7 664,671 + hpaH NOLOHH_03735 0.35 -1.3 664,772 - hpaH NOLOHH_03735 0.48 +0.2 664,786 + hpaH NOLOHH_03735 0.49 +1.5 664,786 + hpaH NOLOHH_03735 0.49 -0.2 664,786 + hpaH NOLOHH_03735 0.49 +0.1 664,786 + hpaH NOLOHH_03735 0.49 -0.4 664,787 - hpaH NOLOHH_03735 0.49 -1.3 664,923 + hpaH NOLOHH_03735 0.66 -1.4 664,924 - hpaH NOLOHH_03735 0.66 +0.7 664,924 - hpaH NOLOHH_03735 0.66 +1.1 664,943 + hpaH NOLOHH_03735 0.69 -0.1 664,943 + hpaH NOLOHH_03735 0.69 -0.9 664,944 - hpaH NOLOHH_03735 0.69 -0.5 664,944 - hpaH NOLOHH_03735 0.69 -0.1 664,944 - hpaH NOLOHH_03735 0.69 +0.8 664,944 - hpaH NOLOHH_03735 0.69 +0.4 664,967 - hpaH NOLOHH_03735 0.72 -1.7 665,062 + hpaH NOLOHH_03735 0.84 -0.9 665,062 + hpaH NOLOHH_03735 0.84 +0.5 665,063 - hpaH NOLOHH_03735 0.84 +0.9 665,063 - hpaH NOLOHH_03735 0.84 +1.1 665,069 + hpaH NOLOHH_03735 0.84 -0.7 665,069 + hpaH NOLOHH_03735 0.84 -3.1 665,069 + hpaH NOLOHH_03735 0.84 +0.5 665,069 + hpaH NOLOHH_03735 0.84 -0.7 665,069 + hpaH NOLOHH_03735 0.84 -1.8 665,070 - hpaH NOLOHH_03735 0.85 -0.4 665,070 - hpaH NOLOHH_03735 0.85 -0.9 665,070 - hpaH NOLOHH_03735 0.85 -0.0 665,070 - hpaH NOLOHH_03735 0.85 -0.8 665,070 - hpaH NOLOHH_03735 0.85 -0.2 665,175 + -0.2 665,279 + +0.5 665,280 - -0.1 665,410 - hpaI NOLOHH_03740 0.26 -1.1 665,410 - hpaI NOLOHH_03740 0.26 +0.8 665,747 + hpaI NOLOHH_03740 0.69 -1.1 665,776 - hpaI NOLOHH_03740 0.72 -1.1 665,868 - hpaI NOLOHH_03740 0.84 +1.1 665,987 + -1.5 665,987 + -0.2 665,988 - -0.1 665,988 - +0.0 665,988 - -0.3 665,990 + -0.9 665,991 - -0.3 666,011 + -0.5 666,012 - -0.6 666,020 + +0.5 666,020 + +1.1 666,020 + -0.3 666,124 - +0.0 666,124 - -0.7 666,124 - -0.1 666,136 - +0.2 666,141 - -0.8 666,190 - -1.4
Or see this region's nucleotide sequence