Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaC and yacM are separated by 155 nucleotides yacM and coaE are separated by 24 nucleotides coaE and zapD overlap by 1 nucleotides
NOLOHH_02895: guaC - GMP reductase, at 494,859 to 495,902
guaC
NOLOHH_02900: yacM - Protein YacM, at 496,058 to 496,102
yacM
NOLOHH_02905: coaE - dephospho-CoA kinase, at 496,127 to 496,747
coaE
NOLOHH_02910: zapD - cell division protein ZapD, at 496,747 to 497,490
zapD
Position (kb) 496
497 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 495.103 kb on + strand, within guaC at 495.345 kb on - strand, within guaC at 495.375 kb on - strand, within guaC at 495.426 kb on + strand, within guaC at 495.426 kb on + strand, within guaC at 495.427 kb on - strand, within guaC at 495.427 kb on - strand, within guaC at 495.427 kb on - strand, within guaC at 495.521 kb on + strand, within guaC at 495.521 kb on + strand, within guaC at 495.521 kb on + strand, within guaC at 495.521 kb on + strand, within guaC at 495.522 kb on - strand, within guaC at 495.522 kb on - strand, within guaC at 495.522 kb on - strand, within guaC at 495.590 kb on + strand, within guaC at 495.641 kb on + strand, within guaC at 495.667 kb on + strand, within guaC at 495.667 kb on + strand, within guaC at 495.667 kb on + strand, within guaC at 495.667 kb on + strand, within guaC at 495.668 kb on - strand, within guaC at 495.668 kb on - strand, within guaC at 495.668 kb on - strand, within guaC at 495.675 kb on - strand, within guaC at 495.728 kb on + strand, within guaC at 495.729 kb on - strand, within guaC at 495.733 kb on - strand, within guaC at 495.733 kb on - strand, within guaC at 495.733 kb on - strand, within guaC at 495.733 kb on - strand, within guaC at 495.733 kb on - strand, within guaC at 495.733 kb on - strand, within guaC at 495.744 kb on - strand, within guaC at 495.834 kb on - strand at 495.896 kb on + strand at 495.927 kb on + strand at 495.927 kb on + strand at 495.929 kb on + strand at 495.929 kb on + strand at 495.935 kb on + strand at 495.964 kb on - strand at 496.008 kb on + strand at 496.013 kb on + strand at 496.137 kb on + strand at 496.137 kb on + strand at 496.137 kb on + strand at 496.137 kb on + strand at 496.137 kb on + strand at 496.138 kb on - strand at 496.138 kb on - strand at 496.138 kb on - strand at 496.745 kb on + strand at 496.746 kb on - strand at 496.778 kb on + strand at 496.779 kb on - strand at 496.794 kb on + strand at 496.794 kb on + strand at 496.794 kb on + strand at 496.794 kb on + strand at 496.795 kb on - strand at 496.895 kb on + strand, within zapD at 496.895 kb on + strand, within zapD at 496.895 kb on + strand, within zapD at 496.896 kb on - strand, within zapD at 496.962 kb on + strand, within zapD at 496.962 kb on + strand, within zapD at 496.962 kb on + strand, within zapD at 496.963 kb on - strand, within zapD at 497.018 kb on + strand, within zapD at 497.048 kb on + strand, within zapD at 497.051 kb on + strand, within zapD at 497.051 kb on + strand, within zapD at 497.052 kb on - strand, within zapD
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 495,103 + guaC NOLOHH_02895 0.23 +1.0 495,345 - guaC NOLOHH_02895 0.47 -0.8 495,375 - guaC NOLOHH_02895 0.49 +0.7 495,426 + guaC NOLOHH_02895 0.54 +0.3 495,426 + guaC NOLOHH_02895 0.54 +0.5 495,427 - guaC NOLOHH_02895 0.54 +1.1 495,427 - guaC NOLOHH_02895 0.54 +0.0 495,427 - guaC NOLOHH_02895 0.54 +0.5 495,521 + guaC NOLOHH_02895 0.63 +0.8 495,521 + guaC NOLOHH_02895 0.63 -0.5 495,521 + guaC NOLOHH_02895 0.63 +0.5 495,521 + guaC NOLOHH_02895 0.63 +0.7 495,522 - guaC NOLOHH_02895 0.64 +1.7 495,522 - guaC NOLOHH_02895 0.64 +1.1 495,522 - guaC NOLOHH_02895 0.64 +1.5 495,590 + guaC NOLOHH_02895 0.70 -0.2 495,641 + guaC NOLOHH_02895 0.75 -0.3 495,667 + guaC NOLOHH_02895 0.77 +1.3 495,667 + guaC NOLOHH_02895 0.77 +0.8 495,667 + guaC NOLOHH_02895 0.77 -1.0 495,667 + guaC NOLOHH_02895 0.77 +0.3 495,668 - guaC NOLOHH_02895 0.77 -2.2 495,668 - guaC NOLOHH_02895 0.77 -0.7 495,668 - guaC NOLOHH_02895 0.77 -1.3 495,675 - guaC NOLOHH_02895 0.78 +0.0 495,728 + guaC NOLOHH_02895 0.83 +1.0 495,729 - guaC NOLOHH_02895 0.83 -1.3 495,733 - guaC NOLOHH_02895 0.84 -0.6 495,733 - guaC NOLOHH_02895 0.84 +0.5 495,733 - guaC NOLOHH_02895 0.84 -3.5 495,733 - guaC NOLOHH_02895 0.84 -1.1 495,733 - guaC NOLOHH_02895 0.84 -0.9 495,733 - guaC NOLOHH_02895 0.84 -1.7 495,744 - guaC NOLOHH_02895 0.85 +0.7 495,834 - -0.6 495,896 + -0.0 495,927 + -0.3 495,927 + -1.1 495,929 + +0.9 495,929 + -1.4 495,935 + +0.8 495,964 - +0.5 496,008 + -1.4 496,013 + -0.3 496,137 + -0.5 496,137 + -0.3 496,137 + -0.2 496,137 + -0.4 496,137 + +0.0 496,138 - -1.1 496,138 - -1.5 496,138 - -1.1 496,745 + +0.5 496,746 - +0.5 496,778 + +0.1 496,779 - -0.5 496,794 + -1.1 496,794 + +2.1 496,794 + +0.8 496,794 + -2.1 496,795 - -0.7 496,895 + zapD NOLOHH_02910 0.20 +0.0 496,895 + zapD NOLOHH_02910 0.20 -1.1 496,895 + zapD NOLOHH_02910 0.20 -1.3 496,896 - zapD NOLOHH_02910 0.20 -0.7 496,962 + zapD NOLOHH_02910 0.29 +0.1 496,962 + zapD NOLOHH_02910 0.29 +0.3 496,962 + zapD NOLOHH_02910 0.29 +0.5 496,963 - zapD NOLOHH_02910 0.29 +0.3 497,018 + zapD NOLOHH_02910 0.36 -0.1 497,048 + zapD NOLOHH_02910 0.40 -0.7 497,051 + zapD NOLOHH_02910 0.41 -0.2 497,051 + zapD NOLOHH_02910 0.41 -0.4 497,052 - zapD NOLOHH_02910 0.41 +0.3
Or see this region's nucleotide sequence