Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speE and speD are separated by 15 nucleotides speD and yacL are separated by 37 nucleotides yacL and acnB are separated by 174 nucleotides
NOLOHH_02810: speE - polyamine aminopropyltransferase, at 472,979 to 473,845
speE
NOLOHH_02815: speD - adenosylmethionine decarboxylase, at 473,861 to 474,655
speD
NOLOHH_02820: yacL - protein YacL, at 474,693 to 475,055
yacL
NOLOHH_02825: acnB - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 475,230 to 477,827
acnB
Position (kb) 474
475
476 Strain fitness (log2 ratio) -1
0
1
2 at 473.964 kb on - strand, within speD at 474.199 kb on + strand, within speD at 474.211 kb on + strand, within speD at 474.211 kb on + strand, within speD at 474.211 kb on + strand, within speD at 474.211 kb on + strand, within speD at 474.211 kb on + strand, within speD at 474.247 kb on + strand, within speD at 474.311 kb on + strand, within speD at 474.342 kb on + strand, within speD at 474.627 kb on - strand at 474.641 kb on - strand at 474.695 kb on - strand at 474.778 kb on + strand, within yacL at 474.779 kb on - strand, within yacL at 474.779 kb on - strand, within yacL at 474.779 kb on - strand, within yacL at 474.782 kb on - strand, within yacL at 474.868 kb on + strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 474.869 kb on - strand, within yacL at 475.007 kb on + strand, within yacL at 475.007 kb on + strand, within yacL at 475.007 kb on + strand, within yacL at 475.008 kb on - strand, within yacL at 475.008 kb on - strand, within yacL at 475.008 kb on - strand, within yacL at 475.008 kb on - strand, within yacL at 475.008 kb on - strand, within yacL at 475.017 kb on + strand, within yacL at 475.018 kb on - strand, within yacL at 475.055 kb on + strand at 475.109 kb on + strand at 475.109 kb on + strand at 475.109 kb on + strand at 475.109 kb on + strand at 475.109 kb on + strand at 475.110 kb on - strand at 475.110 kb on - strand at 475.110 kb on - strand at 475.110 kb on - strand at 475.110 kb on - strand at 475.156 kb on + strand at 475.224 kb on + strand at 475.228 kb on + strand at 475.229 kb on - strand at 475.229 kb on - strand at 475.229 kb on - strand at 475.232 kb on - strand at 475.274 kb on - strand at 475.310 kb on + strand at 475.311 kb on - strand at 475.326 kb on - strand at 475.326 kb on - strand at 475.568 kb on - strand, within acnB at 475.647 kb on - strand, within acnB
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 473,964 - speD NOLOHH_02815 0.13 -0.5 474,199 + speD NOLOHH_02815 0.43 +0.2 474,211 + speD NOLOHH_02815 0.44 +0.5 474,211 + speD NOLOHH_02815 0.44 +0.5 474,211 + speD NOLOHH_02815 0.44 +0.9 474,211 + speD NOLOHH_02815 0.44 -1.1 474,211 + speD NOLOHH_02815 0.44 +0.8 474,247 + speD NOLOHH_02815 0.49 +0.7 474,311 + speD NOLOHH_02815 0.57 +0.4 474,342 + speD NOLOHH_02815 0.61 +0.2 474,627 - +0.2 474,641 - -0.8 474,695 - -0.3 474,778 + yacL NOLOHH_02820 0.23 -0.1 474,779 - yacL NOLOHH_02820 0.24 +0.2 474,779 - yacL NOLOHH_02820 0.24 +0.8 474,779 - yacL NOLOHH_02820 0.24 -0.8 474,782 - yacL NOLOHH_02820 0.25 +0.2 474,868 + yacL NOLOHH_02820 0.48 -0.6 474,869 - yacL NOLOHH_02820 0.48 -0.4 474,869 - yacL NOLOHH_02820 0.48 +0.3 474,869 - yacL NOLOHH_02820 0.48 -0.5 474,869 - yacL NOLOHH_02820 0.48 +0.5 474,869 - yacL NOLOHH_02820 0.48 -0.1 474,869 - yacL NOLOHH_02820 0.48 +0.2 474,869 - yacL NOLOHH_02820 0.48 +2.1 474,869 - yacL NOLOHH_02820 0.48 +0.3 474,869 - yacL NOLOHH_02820 0.48 +0.2 474,869 - yacL NOLOHH_02820 0.48 -1.4 474,869 - yacL NOLOHH_02820 0.48 -0.2 475,007 + yacL NOLOHH_02820 0.87 -0.6 475,007 + yacL NOLOHH_02820 0.87 -1.5 475,007 + yacL NOLOHH_02820 0.87 -0.8 475,008 - yacL NOLOHH_02820 0.87 -1.1 475,008 - yacL NOLOHH_02820 0.87 -1.6 475,008 - yacL NOLOHH_02820 0.87 +0.5 475,008 - yacL NOLOHH_02820 0.87 +0.5 475,008 - yacL NOLOHH_02820 0.87 -0.5 475,017 + yacL NOLOHH_02820 0.89 -0.1 475,018 - yacL NOLOHH_02820 0.90 +0.5 475,055 + +0.5 475,109 + +0.1 475,109 + +0.4 475,109 + -0.3 475,109 + -1.2 475,109 + -0.2 475,110 - -0.9 475,110 - +0.3 475,110 - +2.1 475,110 - -0.4 475,110 - -0.2 475,156 + +0.5 475,224 + +0.5 475,228 + +0.1 475,229 - +0.5 475,229 - +0.3 475,229 - +0.1 475,232 - +0.5 475,274 - +0.9 475,310 + +1.5 475,311 - +2.5 475,326 - +0.3 475,326 - +2.1 475,568 - acnB NOLOHH_02825 0.13 -0.5 475,647 - acnB NOLOHH_02825 0.16 +0.5
Or see this region's nucleotide sequence