Strain Fitness in Escherichia coli ECOR27 around NOLOHH_22020

Experiment: TP12

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmsbA and ycaI are separated by 36 nucleotidesycaI and ihfB are separated by 206 nucleotidesihfB and rpsA are separated by 159 nucleotides NOLOHH_22015: msbA - lipid A ABC transporter ATP-binding protein/permease MsbA, at 4,334,603 to 4,336,351 msbA NOLOHH_22020: ycaI - ComEC family protein, at 4,336,388 to 4,338,652 ycaI NOLOHH_22025: ihfB - integration host factor subunit beta, at 4,338,859 to 4,339,143 ihfB NOLOHH_22030: rpsA - 30S ribosomal protein S1, at 4,339,303 to 4,340,976 rpsA Position (kb) 4336 4337 4338 4339Strain fitness (log2 ratio) -2 -1 0 1 2at 4336.437 kb on - strandat 4336.584 kb on + strandat 4336.631 kb on + strand, within ycaIat 4336.654 kb on + strand, within ycaIat 4336.819 kb on + strand, within ycaIat 4336.819 kb on + strand, within ycaIat 4336.907 kb on + strand, within ycaIat 4337.149 kb on - strand, within ycaIat 4337.207 kb on + strand, within ycaIat 4337.208 kb on - strand, within ycaIat 4337.730 kb on - strand, within ycaIat 4337.730 kb on - strand, within ycaIat 4337.770 kb on + strand, within ycaIat 4337.770 kb on + strand, within ycaIat 4337.785 kb on + strand, within ycaIat 4337.907 kb on + strand, within ycaIat 4337.907 kb on + strand, within ycaIat 4337.908 kb on - strand, within ycaIat 4337.981 kb on + strand, within ycaIat 4338.054 kb on - strand, within ycaIat 4338.064 kb on + strand, within ycaIat 4338.079 kb on + strand, within ycaIat 4338.080 kb on - strand, within ycaIat 4338.082 kb on - strand, within ycaIat 4338.082 kb on - strand, within ycaIat 4338.082 kb on - strand, within ycaIat 4338.101 kb on + strand, within ycaIat 4338.101 kb on + strand, within ycaIat 4338.131 kb on - strand, within ycaIat 4338.226 kb on + strand, within ycaIat 4338.309 kb on + strand, within ycaIat 4338.337 kb on + strand, within ycaIat 4338.338 kb on - strand, within ycaIat 4338.338 kb on - strand, within ycaIat 4338.338 kb on - strand, within ycaIat 4338.350 kb on + strand, within ycaIat 4338.351 kb on - strand, within ycaIat 4338.505 kb on + strandat 4338.507 kb on + strandat 4338.529 kb on + strandat 4338.629 kb on + strandat 4338.867 kb on - strandat 4338.958 kb on - strand, within ihfBat 4339.220 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP12
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4,336,437 - -1.5
4,336,584 + +0.0
4,336,631 + ycaI NOLOHH_22020 0.11 -0.5
4,336,654 + ycaI NOLOHH_22020 0.12 -0.5
4,336,819 + ycaI NOLOHH_22020 0.19 -0.4
4,336,819 + ycaI NOLOHH_22020 0.19 -1.1
4,336,907 + ycaI NOLOHH_22020 0.23 -0.7
4,337,149 - ycaI NOLOHH_22020 0.34 +1.5
4,337,207 + ycaI NOLOHH_22020 0.36 -1.5
4,337,208 - ycaI NOLOHH_22020 0.36 -0.1
4,337,730 - ycaI NOLOHH_22020 0.59 +0.5
4,337,730 - ycaI NOLOHH_22020 0.59 -0.5
4,337,770 + ycaI NOLOHH_22020 0.61 -0.7
4,337,770 + ycaI NOLOHH_22020 0.61 +0.5
4,337,785 + ycaI NOLOHH_22020 0.62 +0.5
4,337,907 + ycaI NOLOHH_22020 0.67 +0.1
4,337,907 + ycaI NOLOHH_22020 0.67 +1.5
4,337,908 - ycaI NOLOHH_22020 0.67 +1.1
4,337,981 + ycaI NOLOHH_22020 0.70 +0.8
4,338,054 - ycaI NOLOHH_22020 0.74 +1.3
4,338,064 + ycaI NOLOHH_22020 0.74 +0.5
4,338,079 + ycaI NOLOHH_22020 0.75 +1.5
4,338,080 - ycaI NOLOHH_22020 0.75 +0.0
4,338,082 - ycaI NOLOHH_22020 0.75 +1.2
4,338,082 - ycaI NOLOHH_22020 0.75 -0.5
4,338,082 - ycaI NOLOHH_22020 0.75 +1.5
4,338,101 + ycaI NOLOHH_22020 0.76 -1.2
4,338,101 + ycaI NOLOHH_22020 0.76 +0.5
4,338,131 - ycaI NOLOHH_22020 0.77 +2.5
4,338,226 + ycaI NOLOHH_22020 0.81 -0.5
4,338,309 + ycaI NOLOHH_22020 0.85 -2.1
4,338,337 + ycaI NOLOHH_22020 0.86 -1.5
4,338,338 - ycaI NOLOHH_22020 0.86 +2.1
4,338,338 - ycaI NOLOHH_22020 0.86 +0.5
4,338,338 - ycaI NOLOHH_22020 0.86 +1.8
4,338,350 + ycaI NOLOHH_22020 0.87 +0.5
4,338,351 - ycaI NOLOHH_22020 0.87 +2.1
4,338,505 + +0.1
4,338,507 + +1.0
4,338,529 + +0.2
4,338,629 + +0.5
4,338,867 - -1.5
4,338,958 - ihfB NOLOHH_22025 0.35 -1.1
4,339,220 - +0.2

Or see this region's nucleotide sequence