Strain Fitness in Escherichia coli ECOR27 around NOLOHH_22010

Experiment: TP12

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycaQ and lpxK are separated by 36 nucleotideslpxK and msbA overlap by 4 nucleotides NOLOHH_22005: ycaQ - Uncharacterized protein YcaQ, at 4,332,351 to 4,333,583 ycaQ NOLOHH_22010: lpxK - tetraacyldisaccharide 4'-kinase, at 4,333,620 to 4,334,606 lpxK NOLOHH_22015: msbA - lipid A ABC transporter ATP-binding protein/permease MsbA, at 4,334,603 to 4,336,351 msbA Position (kb) 4333 4334 4335Strain fitness (log2 ratio) -1 0 1 2 3at 4332.644 kb on - strand, within ycaQat 4332.644 kb on - strand, within ycaQat 4332.663 kb on - strand, within ycaQat 4332.895 kb on + strand, within ycaQat 4333.080 kb on - strand, within ycaQat 4333.198 kb on - strand, within ycaQat 4333.311 kb on - strand, within ycaQat 4333.385 kb on - strand, within ycaQat 4333.414 kb on + strand, within ycaQat 4333.518 kb on + strandat 4333.621 kb on + strandat 4333.621 kb on + strandat 4333.621 kb on + strandat 4333.622 kb on - strandat 4333.622 kb on - strandat 4333.622 kb on - strandat 4333.622 kb on - strandat 4333.622 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP12
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4,332,644 - ycaQ NOLOHH_22005 0.24 +0.5
4,332,644 - ycaQ NOLOHH_22005 0.24 +0.5
4,332,663 - ycaQ NOLOHH_22005 0.25 +0.1
4,332,895 + ycaQ NOLOHH_22005 0.44 +0.1
4,333,080 - ycaQ NOLOHH_22005 0.59 -1.7
4,333,198 - ycaQ NOLOHH_22005 0.69 -1.3
4,333,311 - ycaQ NOLOHH_22005 0.78 -1.1
4,333,385 - ycaQ NOLOHH_22005 0.84 +0.7
4,333,414 + ycaQ NOLOHH_22005 0.86 +0.9
4,333,518 + -1.1
4,333,621 + +1.1
4,333,621 + -1.5
4,333,621 + +0.9
4,333,622 - +0.1
4,333,622 - -0.5
4,333,622 - +0.2
4,333,622 - +1.2
4,333,622 - +3.1

Or see this region's nucleotide sequence