Strain Fitness in Escherichia coli ECOR27 around NOLOHH_17475

Experiment: TP12

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnadE and osmE are separated by 201 nucleotidesosmE and chbB are separated by 298 nucleotideschbB and chbC are separated by 84 nucleotides NOLOHH_17465: nadE - ammonia-dependent NAD(+) synthetase, at 3,428,641 to 3,429,468 nadE NOLOHH_17470: osmE - osmotically-inducible lipoprotein OsmE, at 3,429,670 to 3,430,008 osmE NOLOHH_17475: chbB - PTS N,N'-diacetylchitobiose transporter subunit IIB, at 3,430,307 to 3,430,627 chbB NOLOHH_17480: chbC - PTS N,N'-diacetylchitobiose transporter subunit IIC, at 3,430,712 to 3,432,070 chbC Position (kb) 3430 3431Strain fitness (log2 ratio) -2 -1 0 1 2at 3429.732 kb on + strand, within osmEat 3429.739 kb on + strand, within osmEat 3429.880 kb on + strand, within osmEat 3429.979 kb on + strandat 3430.006 kb on + strandat 3430.031 kb on + strandat 3430.168 kb on + strandat 3430.325 kb on + strandat 3430.855 kb on + strand, within chbCat 3431.104 kb on + strand, within chbCat 3431.120 kb on + strand, within chbC

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Per-strain Table

Position Strand Gene LocusTag Fraction TP12
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3,429,732 + osmE NOLOHH_17470 0.18 -1.8
3,429,739 + osmE NOLOHH_17470 0.20 -2.3
3,429,880 + osmE NOLOHH_17470 0.62 -0.5
3,429,979 + -2.5
3,430,006 + -1.5
3,430,031 + -1.5
3,430,168 + +2.5
3,430,325 + +0.5
3,430,855 + chbC NOLOHH_17480 0.11 +0.5
3,431,104 + chbC NOLOHH_17480 0.29 -1.0
3,431,120 + chbC NOLOHH_17480 0.30 +2.5

Or see this region's nucleotide sequence