Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgi and tal overlap by 4 nucleotides tal and OKGIIK_15530 are separated by 37 nucleotides OKGIIK_15530 and zwf overlap by 7 nucleotides
OKGIIK_15520: pgi - glucose-6-phosphate isomerase, at 3,378,674 to 3,380,323
pgi
OKGIIK_15525: tal - transaldolase, at 3,380,320 to 3,381,414
tal
OKGIIK_15530: OKGIIK_15530 - Haloacid dehalogenase, at 3,381,452 to 3,382,168
_15530
OKGIIK_15535: zwf - glucose-6-phosphate dehydrogenase, at 3,382,162 to 3,383,778
zwf
Position (kb)
3380
3381
3382 Strain fitness (log2 ratio)
-2
-1
0
1 at 3379.813 kb on - strand, within pgi at 3379.813 kb on - strand, within pgi at 3379.830 kb on + strand, within pgi at 3379.831 kb on - strand, within pgi at 3379.831 kb on - strand, within pgi at 3379.831 kb on - strand, within pgi at 3380.170 kb on - strand at 3380.170 kb on - strand at 3380.178 kb on + strand at 3380.178 kb on + strand at 3380.600 kb on + strand, within tal at 3380.600 kb on + strand, within tal at 3380.601 kb on - strand, within tal at 3380.601 kb on - strand, within tal at 3380.601 kb on - strand, within tal at 3380.601 kb on - strand, within tal at 3380.601 kb on - strand, within tal at 3380.601 kb on - strand, within tal at 3380.726 kb on + strand, within tal at 3380.727 kb on - strand, within tal at 3380.727 kb on - strand, within tal at 3380.883 kb on - strand, within tal at 3380.883 kb on - strand, within tal at 3380.883 kb on - strand, within tal at 3380.883 kb on - strand, within tal at 3380.903 kb on + strand, within tal at 3380.904 kb on - strand, within tal at 3380.904 kb on - strand, within tal at 3380.904 kb on - strand, within tal at 3380.904 kb on - strand, within tal at 3381.242 kb on + strand, within tal at 3381.243 kb on - strand, within tal at 3381.243 kb on - strand, within tal at 3381.243 kb on - strand, within tal at 3381.320 kb on + strand at 3381.321 kb on - strand at 3381.443 kb on + strand at 3381.443 kb on + strand at 3381.444 kb on - strand at 3381.444 kb on - strand at 3381.444 kb on - strand at 3381.444 kb on - strand at 3381.444 kb on - strand at 3381.444 kb on - strand at 3381.444 kb on - strand at 3381.444 kb on - strand at 3381.444 kb on - strand at 3381.505 kb on + strand at 3381.505 kb on + strand at 3381.505 kb on + strand at 3381.505 kb on + strand at 3381.506 kb on - strand at 3381.585 kb on + strand, within OKGIIK_15530 at 3381.586 kb on - strand, within OKGIIK_15530 at 3381.678 kb on + strand, within OKGIIK_15530 at 3381.679 kb on - strand, within OKGIIK_15530 at 3381.679 kb on - strand, within OKGIIK_15530 at 3381.679 kb on - strand, within OKGIIK_15530 at 3381.679 kb on - strand, within OKGIIK_15530 at 3381.679 kb on - strand, within OKGIIK_15530 at 3381.679 kb on - strand, within OKGIIK_15530 at 3381.735 kb on + strand, within OKGIIK_15530 at 3381.736 kb on - strand, within OKGIIK_15530 at 3381.736 kb on - strand, within OKGIIK_15530 at 3381.736 kb on - strand, within OKGIIK_15530 at 3381.897 kb on + strand, within OKGIIK_15530 at 3381.897 kb on + strand, within OKGIIK_15530 at 3381.910 kb on - strand, within OKGIIK_15530 at 3381.910 kb on - strand, within OKGIIK_15530 at 3381.910 kb on - strand, within OKGIIK_15530 at 3381.910 kb on - strand, within OKGIIK_15530 at 3382.014 kb on + strand, within OKGIIK_15530 at 3382.015 kb on - strand, within OKGIIK_15530 at 3382.015 kb on - strand, within OKGIIK_15530 at 3382.015 kb on - strand, within OKGIIK_15530 at 3382.026 kb on + strand, within OKGIIK_15530 at 3382.027 kb on - strand, within OKGIIK_15530 at 3382.259 kb on + strand at 3382.259 kb on + strand at 3382.259 kb on + strand at 3382.260 kb on - strand at 3382.260 kb on - strand at 3382.260 kb on - strand at 3382.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 3,379,813 - pgi OKGIIK_15520 0.69 -0.0 3,379,813 - pgi OKGIIK_15520 0.69 -0.5 3,379,830 + pgi OKGIIK_15520 0.70 -1.0 3,379,831 - pgi OKGIIK_15520 0.70 -1.5 3,379,831 - pgi OKGIIK_15520 0.70 -1.7 3,379,831 - pgi OKGIIK_15520 0.70 -1.9 3,380,170 - -1.4 3,380,170 - -1.4 3,380,178 + -1.2 3,380,178 + -1.5 3,380,600 + tal OKGIIK_15525 0.26 -1.7 3,380,600 + tal OKGIIK_15525 0.26 -1.2 3,380,601 - tal OKGIIK_15525 0.26 -0.5 3,380,601 - tal OKGIIK_15525 0.26 +0.8 3,380,601 - tal OKGIIK_15525 0.26 -0.8 3,380,601 - tal OKGIIK_15525 0.26 +0.4 3,380,601 - tal OKGIIK_15525 0.26 -1.9 3,380,601 - tal OKGIIK_15525 0.26 -1.4 3,380,726 + tal OKGIIK_15525 0.37 -0.2 3,380,727 - tal OKGIIK_15525 0.37 -0.4 3,380,727 - tal OKGIIK_15525 0.37 -0.5 3,380,883 - tal OKGIIK_15525 0.51 -0.0 3,380,883 - tal OKGIIK_15525 0.51 -0.5 3,380,883 - tal OKGIIK_15525 0.51 -2.0 3,380,883 - tal OKGIIK_15525 0.51 -2.1 3,380,903 + tal OKGIIK_15525 0.53 -0.3 3,380,904 - tal OKGIIK_15525 0.53 -1.2 3,380,904 - tal OKGIIK_15525 0.53 -0.5 3,380,904 - tal OKGIIK_15525 0.53 -1.2 3,380,904 - tal OKGIIK_15525 0.53 -1.9 3,381,242 + tal OKGIIK_15525 0.84 +1.4 3,381,243 - tal OKGIIK_15525 0.84 -0.2 3,381,243 - tal OKGIIK_15525 0.84 -0.2 3,381,243 - tal OKGIIK_15525 0.84 -0.5 3,381,320 + -1.7 3,381,321 - +0.1 3,381,443 + +1.4 3,381,443 + +0.2 3,381,444 - -0.8 3,381,444 - -0.5 3,381,444 - -1.2 3,381,444 - -1.5 3,381,444 - -0.5 3,381,444 - +1.2 3,381,444 - +1.3 3,381,444 - -1.0 3,381,444 - +0.2 3,381,505 + +1.2 3,381,505 + -1.0 3,381,505 + +0.6 3,381,505 + -1.5 3,381,506 - -1.5 3,381,585 + OKGIIK_15530 0.19 -0.2 3,381,586 - OKGIIK_15530 0.19 -0.7 3,381,678 + OKGIIK_15530 0.32 -1.0 3,381,679 - OKGIIK_15530 0.32 -0.6 3,381,679 - OKGIIK_15530 0.32 -1.2 3,381,679 - OKGIIK_15530 0.32 -0.4 3,381,679 - OKGIIK_15530 0.32 -0.8 3,381,679 - OKGIIK_15530 0.32 -0.6 3,381,679 - OKGIIK_15530 0.32 -0.1 3,381,735 + OKGIIK_15530 0.39 -1.0 3,381,736 - OKGIIK_15530 0.40 -0.1 3,381,736 - OKGIIK_15530 0.40 -1.6 3,381,736 - OKGIIK_15530 0.40 -0.5 3,381,897 + OKGIIK_15530 0.62 -1.9 3,381,897 + OKGIIK_15530 0.62 +0.5 3,381,910 - OKGIIK_15530 0.64 -1.0 3,381,910 - OKGIIK_15530 0.64 +1.1 3,381,910 - OKGIIK_15530 0.64 -1.2 3,381,910 - OKGIIK_15530 0.64 +0.2 3,382,014 + OKGIIK_15530 0.78 -0.9 3,382,015 - OKGIIK_15530 0.79 -0.6 3,382,015 - OKGIIK_15530 0.79 -0.3 3,382,015 - OKGIIK_15530 0.79 -1.2 3,382,026 + OKGIIK_15530 0.80 -0.4 3,382,027 - OKGIIK_15530 0.80 +0.5 3,382,259 + -1.5 3,382,259 + -0.9 3,382,259 + -0.9 3,382,260 - +0.8 3,382,260 - -0.1 3,382,260 - -1.7 3,382,260 - -0.0
Or see this region's nucleotide sequence