Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrB and thrC are separated by 106 nucleotides thrC and leuA are separated by 473 nucleotides
OKGIIK_13565: thrB - homoserine kinase, at 2,939,332 to 2,940,324
thrB
OKGIIK_13570: thrC - threonine synthase, at 2,940,431 to 2,941,747
thrC
OKGIIK_13575: leuA - 2-isopropylmalate synthase, at 2,942,221 to 2,943,876
leuA
Position (kb)
2940
2941
2942 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2939.753 kb on - strand, within thrB at 2940.182 kb on - strand, within thrB at 2940.335 kb on - strand at 2940.335 kb on - strand at 2940.536 kb on + strand at 2941.128 kb on - strand, within thrC at 2941.128 kb on - strand, within thrC at 2941.400 kb on + strand, within thrC at 2941.746 kb on - strand at 2941.746 kb on - strand at 2941.746 kb on - strand at 2941.746 kb on - strand at 2941.746 kb on - strand at 2941.746 kb on - strand at 2941.780 kb on + strand at 2941.780 kb on + strand at 2941.780 kb on + strand at 2941.781 kb on - strand at 2941.781 kb on - strand at 2941.781 kb on - strand at 2941.781 kb on - strand at 2941.782 kb on + strand at 2941.782 kb on + strand at 2941.782 kb on + strand at 2941.782 kb on + strand at 2941.782 kb on + strand at 2941.782 kb on + strand at 2941.782 kb on + strand at 2941.783 kb on - strand at 2941.783 kb on - strand at 2941.783 kb on - strand at 2941.783 kb on - strand at 2941.812 kb on + strand at 2941.812 kb on + strand at 2941.812 kb on + strand at 2941.960 kb on - strand at 2941.960 kb on - strand at 2941.960 kb on - strand at 2942.061 kb on + strand at 2942.061 kb on + strand at 2942.061 kb on + strand at 2942.061 kb on + strand at 2942.061 kb on + strand at 2942.061 kb on + strand at 2942.061 kb on + strand at 2942.062 kb on - strand at 2942.062 kb on - strand at 2942.062 kb on - strand at 2942.062 kb on - strand at 2942.062 kb on - strand at 2942.062 kb on - strand at 2942.062 kb on - strand at 2942.065 kb on - strand at 2942.065 kb on - strand at 2942.065 kb on - strand at 2942.073 kb on - strand at 2942.073 kb on - strand at 2942.073 kb on - strand at 2942.078 kb on + strand at 2942.079 kb on - strand at 2942.085 kb on + strand at 2942.085 kb on + strand at 2942.085 kb on + strand at 2942.086 kb on - strand at 2942.086 kb on - strand at 2942.086 kb on - strand at 2942.086 kb on - strand at 2942.086 kb on - strand at 2942.086 kb on - strand at 2942.088 kb on + strand at 2942.089 kb on - strand at 2942.089 kb on - strand at 2942.126 kb on - strand at 2942.126 kb on - strand at 2942.130 kb on - strand at 2942.130 kb on - strand at 2942.130 kb on - strand at 2942.267 kb on + strand at 2942.268 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 2,939,753 - thrB OKGIIK_13565 0.42 -0.6 2,940,182 - thrB OKGIIK_13565 0.86 -0.4 2,940,335 - +0.7 2,940,335 - -0.1 2,940,536 + -0.5 2,941,128 - thrC OKGIIK_13570 0.53 -0.1 2,941,128 - thrC OKGIIK_13570 0.53 -3.1 2,941,400 + thrC OKGIIK_13570 0.74 -0.5 2,941,746 - -0.3 2,941,746 - -2.5 2,941,746 - +0.5 2,941,746 - +0.4 2,941,746 - +0.3 2,941,746 - +0.1 2,941,780 + +0.3 2,941,780 + +0.1 2,941,780 + -0.9 2,941,781 - +0.9 2,941,781 - +0.3 2,941,781 - -1.8 2,941,781 - -2.1 2,941,782 + -0.9 2,941,782 + -1.0 2,941,782 + -0.9 2,941,782 + +0.6 2,941,782 + -0.0 2,941,782 + -1.3 2,941,782 + -1.9 2,941,783 - -0.9 2,941,783 - +0.9 2,941,783 - -0.6 2,941,783 - -1.7 2,941,812 + +0.3 2,941,812 + -1.2 2,941,812 + -0.3 2,941,960 - +0.2 2,941,960 - -0.9 2,941,960 - -0.5 2,942,061 + -0.7 2,942,061 + +0.4 2,942,061 + -0.7 2,942,061 + -1.4 2,942,061 + +0.2 2,942,061 + -1.2 2,942,061 + -0.8 2,942,062 - +0.6 2,942,062 - -0.1 2,942,062 - -0.1 2,942,062 - +0.3 2,942,062 - -0.1 2,942,062 - -1.3 2,942,062 - -0.3 2,942,065 - +0.7 2,942,065 - +0.5 2,942,065 - -0.5 2,942,073 - -1.3 2,942,073 - -0.1 2,942,073 - -0.7 2,942,078 + -0.1 2,942,079 - -0.1 2,942,085 + +0.4 2,942,085 + +0.1 2,942,085 + -2.1 2,942,086 - -0.1 2,942,086 - +0.5 2,942,086 - -1.8 2,942,086 - -0.4 2,942,086 - -0.9 2,942,086 - -0.5 2,942,088 + -0.1 2,942,089 - +0.3 2,942,089 - -0.1 2,942,126 - +2.1 2,942,126 - +1.1 2,942,130 - -1.9 2,942,130 - -0.5 2,942,130 - -0.6 2,942,267 + +0.6 2,942,268 - -2.0
Or see this region's nucleotide sequence