Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt serB and trpC are separated by 5 nucleotides trpC and trpD are separated by 97 nucleotides
OKGIIK_12275: serB - Haloacid dehalogenase, at 2,637,954 to 2,638,628
serB
OKGIIK_12280: trpC - indole-3-glycerol phosphate synthase TrpC, at 2,638,634 to 2,639,428
trpC
OKGIIK_12285: trpD - anthranilate phosphoribosyltransferase, at 2,639,526 to 2,640,584
trpD
Position (kb)
2638
2639
2640 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2638.072 kb on + strand, within serB at 2638.072 kb on + strand, within serB at 2638.072 kb on + strand, within serB at 2638.072 kb on + strand, within serB at 2638.072 kb on + strand, within serB at 2638.072 kb on + strand, within serB at 2638.072 kb on + strand, within serB at 2638.073 kb on - strand, within serB at 2638.073 kb on - strand, within serB at 2638.073 kb on - strand, within serB at 2638.073 kb on - strand, within serB at 2638.432 kb on + strand, within serB at 2638.432 kb on + strand, within serB at 2638.432 kb on + strand, within serB at 2638.432 kb on + strand, within serB at 2638.432 kb on + strand, within serB at 2638.432 kb on + strand, within serB at 2638.432 kb on + strand, within serB at 2638.433 kb on - strand, within serB at 2638.433 kb on - strand, within serB at 2638.433 kb on - strand, within serB at 2638.433 kb on - strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.543 kb on + strand, within serB at 2638.544 kb on - strand, within serB at 2638.544 kb on - strand, within serB at 2638.734 kb on + strand, within trpC at 2638.734 kb on + strand, within trpC at 2638.735 kb on - strand, within trpC at 2638.788 kb on + strand, within trpC at 2638.788 kb on + strand, within trpC at 2638.789 kb on - strand, within trpC at 2638.789 kb on - strand, within trpC at 2639.181 kb on + strand, within trpC at 2639.182 kb on - strand, within trpC at 2639.447 kb on + strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.448 kb on - strand at 2639.477 kb on - strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.527 kb on + strand at 2639.528 kb on - strand at 2639.528 kb on - strand at 2639.528 kb on - strand at 2639.528 kb on - strand at 2639.656 kb on + strand, within trpD at 2639.657 kb on - strand, within trpD at 2639.657 kb on - strand, within trpD at 2639.815 kb on + strand, within trpD at 2639.815 kb on + strand, within trpD at 2639.816 kb on - strand, within trpD at 2639.816 kb on - strand, within trpD at 2639.816 kb on - strand, within trpD at 2640.049 kb on + strand, within trpD
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 2,638,072 + serB OKGIIK_12275 0.17 -0.3 2,638,072 + serB OKGIIK_12275 0.17 -0.7 2,638,072 + serB OKGIIK_12275 0.17 +0.4 2,638,072 + serB OKGIIK_12275 0.17 -0.6 2,638,072 + serB OKGIIK_12275 0.17 -1.0 2,638,072 + serB OKGIIK_12275 0.17 -1.5 2,638,072 + serB OKGIIK_12275 0.17 -0.3 2,638,073 - serB OKGIIK_12275 0.18 -1.2 2,638,073 - serB OKGIIK_12275 0.18 -0.2 2,638,073 - serB OKGIIK_12275 0.18 -1.2 2,638,073 - serB OKGIIK_12275 0.18 -0.8 2,638,432 + serB OKGIIK_12275 0.71 -0.0 2,638,432 + serB OKGIIK_12275 0.71 +2.8 2,638,432 + serB OKGIIK_12275 0.71 -1.3 2,638,432 + serB OKGIIK_12275 0.71 -1.0 2,638,432 + serB OKGIIK_12275 0.71 -1.4 2,638,432 + serB OKGIIK_12275 0.71 -2.0 2,638,432 + serB OKGIIK_12275 0.71 -2.0 2,638,433 - serB OKGIIK_12275 0.71 -2.2 2,638,433 - serB OKGIIK_12275 0.71 -0.1 2,638,433 - serB OKGIIK_12275 0.71 +0.5 2,638,433 - serB OKGIIK_12275 0.71 +0.1 2,638,543 + serB OKGIIK_12275 0.87 -0.3 2,638,543 + serB OKGIIK_12275 0.87 -1.3 2,638,543 + serB OKGIIK_12275 0.87 -0.4 2,638,543 + serB OKGIIK_12275 0.87 -1.3 2,638,543 + serB OKGIIK_12275 0.87 -2.6 2,638,543 + serB OKGIIK_12275 0.87 -1.8 2,638,543 + serB OKGIIK_12275 0.87 +0.8 2,638,543 + serB OKGIIK_12275 0.87 -0.5 2,638,544 - serB OKGIIK_12275 0.87 -0.9 2,638,544 - serB OKGIIK_12275 0.87 +0.0 2,638,734 + trpC OKGIIK_12280 0.13 -1.3 2,638,734 + trpC OKGIIK_12280 0.13 -1.4 2,638,735 - trpC OKGIIK_12280 0.13 -1.6 2,638,788 + trpC OKGIIK_12280 0.19 +0.0 2,638,788 + trpC OKGIIK_12280 0.19 +0.3 2,638,789 - trpC OKGIIK_12280 0.19 -0.9 2,638,789 - trpC OKGIIK_12280 0.19 -1.4 2,639,181 + trpC OKGIIK_12280 0.69 -2.2 2,639,182 - trpC OKGIIK_12280 0.69 -0.2 2,639,447 + -0.3 2,639,448 - -2.2 2,639,448 - -0.1 2,639,448 - -0.7 2,639,448 - -0.9 2,639,448 - +0.0 2,639,477 - -0.6 2,639,527 + -0.2 2,639,527 + +0.3 2,639,527 + +1.4 2,639,527 + +0.4 2,639,527 + -0.6 2,639,527 + +1.4 2,639,527 + +0.1 2,639,527 + -1.1 2,639,527 + +0.7 2,639,528 - +1.0 2,639,528 - +0.2 2,639,528 - -0.3 2,639,528 - -0.8 2,639,656 + trpD OKGIIK_12285 0.12 -2.2 2,639,657 - trpD OKGIIK_12285 0.12 +0.4 2,639,657 - trpD OKGIIK_12285 0.12 -1.3 2,639,815 + trpD OKGIIK_12285 0.27 -2.4 2,639,815 + trpD OKGIIK_12285 0.27 -0.8 2,639,816 - trpD OKGIIK_12285 0.27 +1.0 2,639,816 - trpD OKGIIK_12285 0.27 +1.6 2,639,816 - trpD OKGIIK_12285 0.27 -1.2 2,640,049 + trpD OKGIIK_12285 0.49 +0.0
Or see this region's nucleotide sequence