Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_11495 and OKGIIK_11500 are separated by 130 nucleotides OKGIIK_11500 and purL are separated by 29 nucleotides purL and dsbG are separated by 112 nucleotides
OKGIIK_11495: OKGIIK_11495 - Glycosyl transferase family 2, at 2,466,892 to 2,467,734
_11495
OKGIIK_11500: OKGIIK_11500 - hypothetical protein, at 2,467,865 to 2,468,611
_11500
OKGIIK_11505: purL - phosphoribosylformylglycinamidine synthase, at 2,468,641 to 2,472,507
purL
OKGIIK_11510: dsbG - disulfide bond formation protein DsbC, at 2,472,620 to 2,473,393
dsbG
Position (kb)
2468
2469
2470
2471
2472
2473 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2467.791 kb on + strand at 2467.791 kb on + strand at 2467.791 kb on + strand at 2467.791 kb on + strand at 2467.791 kb on + strand at 2467.791 kb on + strand at 2467.791 kb on + strand at 2467.791 kb on + strand at 2467.791 kb on + strand at 2467.791 kb on + strand at 2467.792 kb on - strand at 2467.792 kb on - strand at 2467.792 kb on - strand at 2467.792 kb on - strand at 2467.792 kb on - strand at 2467.982 kb on + strand, within OKGIIK_11500 at 2467.982 kb on + strand, within OKGIIK_11500 at 2467.982 kb on + strand, within OKGIIK_11500 at 2467.982 kb on + strand, within OKGIIK_11500 at 2467.983 kb on - strand, within OKGIIK_11500 at 2467.983 kb on - strand, within OKGIIK_11500 at 2467.983 kb on - strand, within OKGIIK_11500 at 2467.983 kb on - strand, within OKGIIK_11500 at 2467.983 kb on - strand, within OKGIIK_11500 at 2467.983 kb on - strand, within OKGIIK_11500 at 2467.983 kb on - strand, within OKGIIK_11500 at 2468.139 kb on + strand, within OKGIIK_11500 at 2468.139 kb on + strand, within OKGIIK_11500 at 2468.140 kb on - strand, within OKGIIK_11500 at 2468.140 kb on - strand, within OKGIIK_11500 at 2468.140 kb on - strand, within OKGIIK_11500 at 2469.873 kb on - strand, within purL at 2470.874 kb on + strand, within purL at 2470.874 kb on + strand, within purL at 2470.874 kb on + strand, within purL at 2470.874 kb on + strand, within purL at 2470.874 kb on + strand, within purL at 2470.875 kb on - strand, within purL at 2470.875 kb on - strand, within purL at 2470.875 kb on - strand, within purL at 2470.875 kb on - strand, within purL at 2471.345 kb on + strand, within purL at 2471.345 kb on + strand, within purL at 2471.346 kb on - strand, within purL at 2471.357 kb on + strand, within purL at 2471.754 kb on - strand, within purL at 2471.766 kb on + strand, within purL at 2471.766 kb on + strand, within purL at 2471.766 kb on + strand, within purL at 2471.766 kb on + strand, within purL at 2471.767 kb on - strand, within purL at 2471.767 kb on - strand, within purL at 2471.939 kb on + strand, within purL at 2471.940 kb on - strand, within purL at 2471.954 kb on + strand, within purL at 2471.955 kb on - strand, within purL at 2472.396 kb on - strand at 2472.499 kb on + strand at 2472.500 kb on - strand at 2472.539 kb on + strand at 2472.539 kb on + strand at 2472.540 kb on - strand at 2472.540 kb on - strand at 2472.540 kb on - strand at 2472.540 kb on - strand at 2472.544 kb on + strand at 2472.544 kb on + strand at 2472.544 kb on + strand at 2472.544 kb on + strand at 2472.544 kb on + strand at 2472.544 kb on + strand at 2472.544 kb on + strand at 2472.544 kb on + strand at 2472.544 kb on + strand at 2472.544 kb on + strand at 2472.545 kb on - strand at 2472.545 kb on - strand at 2472.545 kb on - strand at 2472.545 kb on - strand at 2472.545 kb on - strand at 2472.545 kb on - strand at 2472.545 kb on - strand at 2472.545 kb on - strand at 2472.545 kb on - strand at 2472.545 kb on - strand at 2472.549 kb on + strand at 2472.549 kb on + strand at 2472.550 kb on - strand at 2472.550 kb on - strand at 2472.550 kb on - strand at 2472.550 kb on - strand at 2472.550 kb on - strand at 2472.559 kb on + strand at 2472.559 kb on + strand at 2472.560 kb on - strand at 2472.566 kb on + strand at 2472.566 kb on + strand at 2472.566 kb on + strand at 2472.566 kb on + strand at 2472.567 kb on - strand at 2472.567 kb on - strand at 2472.666 kb on + strand at 2472.666 kb on + strand at 2472.666 kb on + strand at 2472.666 kb on + strand at 2472.666 kb on + strand at 2472.666 kb on + strand at 2472.666 kb on + strand at 2472.667 kb on - strand at 2472.667 kb on - strand at 2472.667 kb on - strand at 2472.667 kb on - strand at 2472.667 kb on - strand at 2472.667 kb on - strand at 2472.696 kb on + strand at 2472.696 kb on + strand at 2472.696 kb on + strand at 2472.697 kb on - strand at 2472.697 kb on - strand at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.855 kb on + strand, within dsbG at 2472.856 kb on - strand, within dsbG at 2472.856 kb on - strand, within dsbG at 2472.856 kb on - strand, within dsbG at 2472.856 kb on - strand, within dsbG at 2472.856 kb on - strand, within dsbG at 2472.912 kb on + strand, within dsbG at 2472.912 kb on + strand, within dsbG at 2472.913 kb on - strand, within dsbG at 2472.913 kb on - strand, within dsbG at 2472.913 kb on - strand, within dsbG at 2472.913 kb on - strand, within dsbG at 2472.913 kb on - strand, within dsbG at 2472.913 kb on - strand, within dsbG at 2472.913 kb on - strand, within dsbG at 2472.913 kb on - strand, within dsbG at 2472.913 kb on - strand, within dsbG at 2473.008 kb on + strand, within dsbG at 2473.008 kb on + strand, within dsbG at 2473.008 kb on + strand, within dsbG at 2473.008 kb on + strand, within dsbG at 2473.008 kb on + strand, within dsbG at 2473.008 kb on + strand, within dsbG at 2473.009 kb on - strand, within dsbG at 2473.009 kb on - strand, within dsbG at 2473.009 kb on - strand, within dsbG at 2473.009 kb on - strand, within dsbG at 2473.009 kb on - strand, within dsbG at 2473.009 kb on - strand, within dsbG at 2473.146 kb on + strand, within dsbG at 2473.146 kb on + strand, within dsbG at 2473.146 kb on + strand, within dsbG at 2473.146 kb on + strand, within dsbG at 2473.147 kb on - strand, within dsbG at 2473.147 kb on - strand, within dsbG at 2473.147 kb on - strand, within dsbG at 2473.147 kb on - strand, within dsbG at 2473.147 kb on - strand, within dsbG at 2473.147 kb on - strand, within dsbG at 2473.147 kb on - strand, within dsbG at 2473.167 kb on + strand, within dsbG at 2473.167 kb on + strand, within dsbG at 2473.167 kb on + strand, within dsbG at 2473.167 kb on + strand, within dsbG at 2473.167 kb on + strand, within dsbG at 2473.167 kb on + strand, within dsbG at 2473.167 kb on + strand, within dsbG at 2473.168 kb on - strand, within dsbG at 2473.168 kb on - strand, within dsbG at 2473.168 kb on - strand, within dsbG at 2473.168 kb on - strand, within dsbG at 2473.168 kb on - strand, within dsbG at 2473.168 kb on - strand, within dsbG at 2473.168 kb on - strand, within dsbG at 2473.168 kb on - strand, within dsbG at 2473.197 kb on + strand, within dsbG at 2473.197 kb on + strand, within dsbG at 2473.197 kb on + strand, within dsbG at 2473.198 kb on - strand, within dsbG at 2473.198 kb on - strand, within dsbG at 2473.198 kb on - strand, within dsbG at 2473.198 kb on - strand, within dsbG at 2473.198 kb on - strand, within dsbG at 2473.198 kb on - strand, within dsbG at 2473.255 kb on + strand, within dsbG at 2473.256 kb on - strand, within dsbG at 2473.256 kb on - strand, within dsbG
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 2,467,791 + +0.0 2,467,791 + -0.9 2,467,791 + -0.3 2,467,791 + -0.0 2,467,791 + -1.4 2,467,791 + +0.5 2,467,791 + -0.0 2,467,791 + -1.6 2,467,791 + +0.1 2,467,791 + +0.1 2,467,792 - -2.2 2,467,792 - +0.5 2,467,792 - +1.1 2,467,792 - +0.5 2,467,792 - -0.3 2,467,982 + OKGIIK_11500 0.16 +1.5 2,467,982 + OKGIIK_11500 0.16 +0.4 2,467,982 + OKGIIK_11500 0.16 -0.5 2,467,982 + OKGIIK_11500 0.16 -1.3 2,467,983 - OKGIIK_11500 0.16 -0.3 2,467,983 - OKGIIK_11500 0.16 -0.2 2,467,983 - OKGIIK_11500 0.16 +0.3 2,467,983 - OKGIIK_11500 0.16 +1.4 2,467,983 - OKGIIK_11500 0.16 -1.5 2,467,983 - OKGIIK_11500 0.16 -1.3 2,467,983 - OKGIIK_11500 0.16 -0.8 2,468,139 + OKGIIK_11500 0.37 +0.3 2,468,139 + OKGIIK_11500 0.37 -1.0 2,468,140 - OKGIIK_11500 0.37 -1.6 2,468,140 - OKGIIK_11500 0.37 -0.5 2,468,140 - OKGIIK_11500 0.37 -0.5 2,469,873 - purL OKGIIK_11505 0.32 +1.1 2,470,874 + purL OKGIIK_11505 0.58 +0.1 2,470,874 + purL OKGIIK_11505 0.58 -0.9 2,470,874 + purL OKGIIK_11505 0.58 -0.5 2,470,874 + purL OKGIIK_11505 0.58 -0.2 2,470,874 + purL OKGIIK_11505 0.58 -0.2 2,470,875 - purL OKGIIK_11505 0.58 -0.2 2,470,875 - purL OKGIIK_11505 0.58 +0.1 2,470,875 - purL OKGIIK_11505 0.58 +0.1 2,470,875 - purL OKGIIK_11505 0.58 -0.5 2,471,345 + purL OKGIIK_11505 0.70 +0.1 2,471,345 + purL OKGIIK_11505 0.70 -0.2 2,471,346 - purL OKGIIK_11505 0.70 -0.9 2,471,357 + purL OKGIIK_11505 0.70 -1.0 2,471,754 - purL OKGIIK_11505 0.81 -0.8 2,471,766 + purL OKGIIK_11505 0.81 -1.5 2,471,766 + purL OKGIIK_11505 0.81 +0.1 2,471,766 + purL OKGIIK_11505 0.81 +1.1 2,471,766 + purL OKGIIK_11505 0.81 +0.1 2,471,767 - purL OKGIIK_11505 0.81 -1.8 2,471,767 - purL OKGIIK_11505 0.81 -0.5 2,471,939 + purL OKGIIK_11505 0.85 -0.4 2,471,940 - purL OKGIIK_11505 0.85 -2.0 2,471,954 + purL OKGIIK_11505 0.86 -1.6 2,471,955 - purL OKGIIK_11505 0.86 +0.1 2,472,396 - -2.0 2,472,499 + +1.4 2,472,500 - +1.3 2,472,539 + +2.1 2,472,539 + -1.0 2,472,540 - +0.6 2,472,540 - +0.2 2,472,540 - +0.3 2,472,540 - -1.2 2,472,544 + +0.1 2,472,544 + -0.5 2,472,544 + -0.6 2,472,544 + +0.1 2,472,544 + +1.1 2,472,544 + -0.7 2,472,544 + -2.4 2,472,544 + +0.6 2,472,544 + -0.1 2,472,544 + -1.7 2,472,545 - +0.1 2,472,545 - -0.4 2,472,545 - -0.7 2,472,545 - -0.4 2,472,545 - -1.2 2,472,545 - +0.5 2,472,545 - +0.0 2,472,545 - +1.1 2,472,545 - +0.1 2,472,545 - +1.4 2,472,549 + -0.3 2,472,549 + -0.3 2,472,550 - -0.6 2,472,550 - +1.2 2,472,550 - +0.5 2,472,550 - +0.7 2,472,550 - -1.0 2,472,559 + -0.9 2,472,559 + -0.7 2,472,560 - +2.1 2,472,566 + -0.3 2,472,566 + -0.6 2,472,566 + +1.7 2,472,566 + +0.6 2,472,567 - -1.8 2,472,567 - -1.0 2,472,666 + -0.1 2,472,666 + +1.7 2,472,666 + -1.8 2,472,666 + +1.9 2,472,666 + -2.2 2,472,666 + -0.4 2,472,666 + -0.4 2,472,667 - +1.1 2,472,667 - +0.3 2,472,667 - -0.3 2,472,667 - -1.9 2,472,667 - +0.6 2,472,667 - -0.6 2,472,696 + -1.9 2,472,696 + -1.1 2,472,696 + -1.0 2,472,697 - -0.0 2,472,697 - -0.4 2,472,855 + dsbG OKGIIK_11510 0.30 -0.2 2,472,855 + dsbG OKGIIK_11510 0.30 +0.1 2,472,855 + dsbG OKGIIK_11510 0.30 +0.1 2,472,855 + dsbG OKGIIK_11510 0.30 +0.6 2,472,855 + dsbG OKGIIK_11510 0.30 -0.0 2,472,855 + dsbG OKGIIK_11510 0.30 +2.1 2,472,855 + dsbG OKGIIK_11510 0.30 -2.0 2,472,855 + dsbG OKGIIK_11510 0.30 -3.0 2,472,855 + dsbG OKGIIK_11510 0.30 -1.6 2,472,855 + dsbG OKGIIK_11510 0.30 +0.1 2,472,855 + dsbG OKGIIK_11510 0.30 -0.6 2,472,855 + dsbG OKGIIK_11510 0.30 -1.4 2,472,855 + dsbG OKGIIK_11510 0.30 +0.8 2,472,855 + dsbG OKGIIK_11510 0.30 -0.4 2,472,855 + dsbG OKGIIK_11510 0.30 +0.1 2,472,856 - dsbG OKGIIK_11510 0.30 +0.1 2,472,856 - dsbG OKGIIK_11510 0.30 -0.4 2,472,856 - dsbG OKGIIK_11510 0.30 -0.4 2,472,856 - dsbG OKGIIK_11510 0.30 +1.1 2,472,856 - dsbG OKGIIK_11510 0.30 -0.9 2,472,912 + dsbG OKGIIK_11510 0.38 -0.2 2,472,912 + dsbG OKGIIK_11510 0.38 +0.7 2,472,913 - dsbG OKGIIK_11510 0.38 +0.7 2,472,913 - dsbG OKGIIK_11510 0.38 +1.3 2,472,913 - dsbG OKGIIK_11510 0.38 -0.4 2,472,913 - dsbG OKGIIK_11510 0.38 +0.1 2,472,913 - dsbG OKGIIK_11510 0.38 -1.0 2,472,913 - dsbG OKGIIK_11510 0.38 -0.7 2,472,913 - dsbG OKGIIK_11510 0.38 +0.9 2,472,913 - dsbG OKGIIK_11510 0.38 -0.3 2,472,913 - dsbG OKGIIK_11510 0.38 +0.3 2,473,008 + dsbG OKGIIK_11510 0.50 +0.7 2,473,008 + dsbG OKGIIK_11510 0.50 -0.2 2,473,008 + dsbG OKGIIK_11510 0.50 +0.3 2,473,008 + dsbG OKGIIK_11510 0.50 -0.3 2,473,008 + dsbG OKGIIK_11510 0.50 -1.2 2,473,008 + dsbG OKGIIK_11510 0.50 +0.3 2,473,009 - dsbG OKGIIK_11510 0.50 +0.1 2,473,009 - dsbG OKGIIK_11510 0.50 -0.2 2,473,009 - dsbG OKGIIK_11510 0.50 +0.3 2,473,009 - dsbG OKGIIK_11510 0.50 -0.6 2,473,009 - dsbG OKGIIK_11510 0.50 -1.5 2,473,009 - dsbG OKGIIK_11510 0.50 +0.1 2,473,146 + dsbG OKGIIK_11510 0.68 +0.6 2,473,146 + dsbG OKGIIK_11510 0.68 -0.3 2,473,146 + dsbG OKGIIK_11510 0.68 +1.1 2,473,146 + dsbG OKGIIK_11510 0.68 +0.8 2,473,147 - dsbG OKGIIK_11510 0.68 -1.7 2,473,147 - dsbG OKGIIK_11510 0.68 +2.1 2,473,147 - dsbG OKGIIK_11510 0.68 +0.1 2,473,147 - dsbG OKGIIK_11510 0.68 -0.4 2,473,147 - dsbG OKGIIK_11510 0.68 -0.2 2,473,147 - dsbG OKGIIK_11510 0.68 +0.0 2,473,147 - dsbG OKGIIK_11510 0.68 +0.6 2,473,167 + dsbG OKGIIK_11510 0.71 -1.4 2,473,167 + dsbG OKGIIK_11510 0.71 -0.3 2,473,167 + dsbG OKGIIK_11510 0.71 -0.1 2,473,167 + dsbG OKGIIK_11510 0.71 -1.5 2,473,167 + dsbG OKGIIK_11510 0.71 -0.7 2,473,167 + dsbG OKGIIK_11510 0.71 -0.4 2,473,167 + dsbG OKGIIK_11510 0.71 +0.7 2,473,168 - dsbG OKGIIK_11510 0.71 +0.1 2,473,168 - dsbG OKGIIK_11510 0.71 +1.8 2,473,168 - dsbG OKGIIK_11510 0.71 -0.1 2,473,168 - dsbG OKGIIK_11510 0.71 +0.9 2,473,168 - dsbG OKGIIK_11510 0.71 +0.1 2,473,168 - dsbG OKGIIK_11510 0.71 -1.8 2,473,168 - dsbG OKGIIK_11510 0.71 -1.4 2,473,168 - dsbG OKGIIK_11510 0.71 -0.4 2,473,197 + dsbG OKGIIK_11510 0.75 -0.6 2,473,197 + dsbG OKGIIK_11510 0.75 +0.1 2,473,197 + dsbG OKGIIK_11510 0.75 -0.2 2,473,198 - dsbG OKGIIK_11510 0.75 +0.1 2,473,198 - dsbG OKGIIK_11510 0.75 -0.0 2,473,198 - dsbG OKGIIK_11510 0.75 -1.0 2,473,198 - dsbG OKGIIK_11510 0.75 +0.0 2,473,198 - dsbG OKGIIK_11510 0.75 -0.0 2,473,198 - dsbG OKGIIK_11510 0.75 -1.6 2,473,255 + dsbG OKGIIK_11510 0.82 -1.8 2,473,256 - dsbG OKGIIK_11510 0.82 +0.9 2,473,256 - dsbG OKGIIK_11510 0.82 -1.9
Or see this region's nucleotide sequence