Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ribD and nrdR are separated by 95 nucleotides nrdR and glyA are separated by 96 nucleotides glyA and ettA are separated by 190 nucleotides
OKGIIK_11430: ribD - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 2,454,344 to 2,455,462
ribD
OKGIIK_11435: nrdR - transcriptional regulator NrdR, at 2,455,558 to 2,456,070
nrdR
OKGIIK_11440: glyA - serine hydroxymethyltransferase, at 2,456,167 to 2,457,435
glyA
OKGIIK_11445: ettA - energy-dependent translational throttle protein EttA, at 2,457,626 to 2,459,293
ettA
Position (kb)
2456
2457
2458 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2455.450 kb on - strand at 2455.479 kb on - strand at 2455.479 kb on - strand at 2455.479 kb on - strand at 2455.479 kb on - strand at 2455.479 kb on - strand at 2455.479 kb on - strand at 2455.545 kb on + strand at 2455.545 kb on + strand at 2455.545 kb on + strand at 2455.545 kb on + strand at 2455.545 kb on + strand at 2455.679 kb on + strand, within nrdR at 2455.679 kb on + strand, within nrdR at 2455.680 kb on - strand, within nrdR at 2455.700 kb on + strand, within nrdR at 2455.701 kb on - strand, within nrdR at 2455.701 kb on - strand, within nrdR at 2455.701 kb on - strand, within nrdR at 2455.701 kb on - strand, within nrdR at 2455.701 kb on - strand, within nrdR at 2455.808 kb on - strand, within nrdR at 2455.808 kb on - strand, within nrdR at 2456.071 kb on - strand at 2456.071 kb on - strand at 2456.071 kb on - strand at 2456.071 kb on - strand at 2456.088 kb on + strand at 2456.089 kb on - strand at 2456.089 kb on - strand at 2456.174 kb on + strand at 2456.175 kb on - strand at 2456.300 kb on + strand, within glyA at 2456.300 kb on + strand, within glyA at 2456.300 kb on + strand, within glyA at 2456.301 kb on - strand, within glyA at 2456.301 kb on - strand, within glyA at 2456.301 kb on - strand, within glyA at 2456.301 kb on - strand, within glyA at 2456.310 kb on + strand, within glyA at 2456.561 kb on + strand, within glyA at 2456.561 kb on + strand, within glyA at 2456.561 kb on + strand, within glyA at 2456.561 kb on + strand, within glyA at 2456.561 kb on + strand, within glyA at 2456.562 kb on - strand, within glyA at 2456.562 kb on - strand, within glyA at 2456.562 kb on - strand, within glyA at 2456.562 kb on - strand, within glyA at 2456.849 kb on + strand, within glyA at 2456.849 kb on + strand, within glyA at 2456.850 kb on - strand, within glyA at 2456.850 kb on - strand, within glyA at 2456.850 kb on - strand, within glyA at 2456.850 kb on - strand, within glyA at 2456.906 kb on + strand, within glyA at 2456.907 kb on - strand, within glyA at 2456.972 kb on + strand, within glyA at 2456.973 kb on - strand, within glyA at 2457.002 kb on + strand, within glyA at 2457.002 kb on + strand, within glyA at 2457.002 kb on + strand, within glyA at 2457.003 kb on - strand, within glyA at 2457.003 kb on - strand, within glyA at 2457.119 kb on + strand, within glyA at 2457.119 kb on + strand, within glyA at 2457.120 kb on - strand, within glyA at 2457.120 kb on - strand, within glyA at 2457.132 kb on + strand, within glyA at 2457.167 kb on + strand, within glyA at 2457.167 kb on + strand, within glyA at 2457.228 kb on - strand, within glyA at 2457.228 kb on - strand, within glyA at 2457.228 kb on - strand, within glyA at 2457.228 kb on - strand, within glyA at 2457.228 kb on - strand, within glyA at 2457.242 kb on + strand, within glyA at 2457.243 kb on - strand, within glyA at 2457.243 kb on - strand, within glyA at 2457.245 kb on + strand, within glyA at 2457.246 kb on - strand, within glyA at 2457.246 kb on - strand, within glyA at 2457.246 kb on - strand, within glyA at 2457.246 kb on - strand, within glyA at 2457.272 kb on + strand, within glyA at 2457.272 kb on + strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.323 kb on + strand at 2457.323 kb on + strand at 2457.323 kb on + strand at 2457.323 kb on + strand at 2457.323 kb on + strand at 2457.323 kb on + strand at 2457.323 kb on + strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.405 kb on - strand at 2457.550 kb on + strand at 2457.550 kb on + strand at 2457.550 kb on + strand at 2457.550 kb on + strand at 2457.551 kb on - strand at 2457.551 kb on - strand at 2457.551 kb on - strand at 2457.552 kb on + strand at 2457.553 kb on - strand at 2457.590 kb on + strand at 2457.590 kb on + strand at 2457.590 kb on + strand at 2457.591 kb on - strand at 2457.591 kb on - strand at 2457.591 kb on - strand at 2457.638 kb on + strand at 2457.638 kb on + strand at 2457.639 kb on - strand at 2457.639 kb on - strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.645 kb on - strand at 2457.645 kb on - strand at 2457.645 kb on - strand at 2457.846 kb on - strand, within ettA at 2457.885 kb on + strand, within ettA at 2457.886 kb on - strand, within ettA at 2457.950 kb on + strand, within ettA at 2457.959 kb on + strand, within ettA at 2458.155 kb on + strand, within ettA at 2458.155 kb on + strand, within ettA at 2458.156 kb on - strand, within ettA at 2458.286 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.359 kb on - strand, within ettA at 2458.359 kb on - strand, within ettA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 2,455,450 - +1.3 2,455,479 - +0.3 2,455,479 - -0.7 2,455,479 - -0.8 2,455,479 - +1.4 2,455,479 - -0.1 2,455,479 - -0.9 2,455,545 + +3.0 2,455,545 + -0.3 2,455,545 + -0.9 2,455,545 + +1.4 2,455,545 + -0.2 2,455,679 + nrdR OKGIIK_11435 0.24 -0.8 2,455,679 + nrdR OKGIIK_11435 0.24 +1.6 2,455,680 - nrdR OKGIIK_11435 0.24 -3.1 2,455,700 + nrdR OKGIIK_11435 0.28 +1.6 2,455,701 - nrdR OKGIIK_11435 0.28 +1.0 2,455,701 - nrdR OKGIIK_11435 0.28 +1.1 2,455,701 - nrdR OKGIIK_11435 0.28 -0.0 2,455,701 - nrdR OKGIIK_11435 0.28 -0.7 2,455,701 - nrdR OKGIIK_11435 0.28 -0.2 2,455,808 - nrdR OKGIIK_11435 0.49 -0.1 2,455,808 - nrdR OKGIIK_11435 0.49 -0.0 2,456,071 - +0.1 2,456,071 - +0.6 2,456,071 - -0.0 2,456,071 - -0.6 2,456,088 + -0.2 2,456,089 - +0.2 2,456,089 - +1.1 2,456,174 + +1.1 2,456,175 - -1.0 2,456,300 + glyA OKGIIK_11440 0.10 +0.3 2,456,300 + glyA OKGIIK_11440 0.10 -1.2 2,456,300 + glyA OKGIIK_11440 0.10 +0.4 2,456,301 - glyA OKGIIK_11440 0.11 +0.1 2,456,301 - glyA OKGIIK_11440 0.11 -0.4 2,456,301 - glyA OKGIIK_11440 0.11 +0.1 2,456,301 - glyA OKGIIK_11440 0.11 +0.4 2,456,310 + glyA OKGIIK_11440 0.11 -0.4 2,456,561 + glyA OKGIIK_11440 0.31 -0.4 2,456,561 + glyA OKGIIK_11440 0.31 +1.7 2,456,561 + glyA OKGIIK_11440 0.31 -0.9 2,456,561 + glyA OKGIIK_11440 0.31 +1.0 2,456,561 + glyA OKGIIK_11440 0.31 -1.8 2,456,562 - glyA OKGIIK_11440 0.31 -1.2 2,456,562 - glyA OKGIIK_11440 0.31 -2.0 2,456,562 - glyA OKGIIK_11440 0.31 -0.7 2,456,562 - glyA OKGIIK_11440 0.31 -0.4 2,456,849 + glyA OKGIIK_11440 0.54 -0.2 2,456,849 + glyA OKGIIK_11440 0.54 +0.1 2,456,850 - glyA OKGIIK_11440 0.54 -0.4 2,456,850 - glyA OKGIIK_11440 0.54 -1.3 2,456,850 - glyA OKGIIK_11440 0.54 -1.7 2,456,850 - glyA OKGIIK_11440 0.54 -0.0 2,456,906 + glyA OKGIIK_11440 0.58 +0.6 2,456,907 - glyA OKGIIK_11440 0.58 -0.9 2,456,972 + glyA OKGIIK_11440 0.63 -2.0 2,456,973 - glyA OKGIIK_11440 0.64 -1.0 2,457,002 + glyA OKGIIK_11440 0.66 -0.4 2,457,002 + glyA OKGIIK_11440 0.66 -0.9 2,457,002 + glyA OKGIIK_11440 0.66 +2.1 2,457,003 - glyA OKGIIK_11440 0.66 -0.2 2,457,003 - glyA OKGIIK_11440 0.66 -1.0 2,457,119 + glyA OKGIIK_11440 0.75 +0.1 2,457,119 + glyA OKGIIK_11440 0.75 -1.9 2,457,120 - glyA OKGIIK_11440 0.75 -0.2 2,457,120 - glyA OKGIIK_11440 0.75 +0.3 2,457,132 + glyA OKGIIK_11440 0.76 +0.7 2,457,167 + glyA OKGIIK_11440 0.79 -0.2 2,457,167 + glyA OKGIIK_11440 0.79 -0.4 2,457,228 - glyA OKGIIK_11440 0.84 -0.9 2,457,228 - glyA OKGIIK_11440 0.84 +0.1 2,457,228 - glyA OKGIIK_11440 0.84 -0.2 2,457,228 - glyA OKGIIK_11440 0.84 -2.4 2,457,228 - glyA OKGIIK_11440 0.84 -0.4 2,457,242 + glyA OKGIIK_11440 0.85 -0.9 2,457,243 - glyA OKGIIK_11440 0.85 -0.3 2,457,243 - glyA OKGIIK_11440 0.85 -2.4 2,457,245 + glyA OKGIIK_11440 0.85 +1.6 2,457,246 - glyA OKGIIK_11440 0.85 +1.7 2,457,246 - glyA OKGIIK_11440 0.85 -0.7 2,457,246 - glyA OKGIIK_11440 0.85 +0.1 2,457,246 - glyA OKGIIK_11440 0.85 -1.0 2,457,272 + glyA OKGIIK_11440 0.87 -0.2 2,457,272 + glyA OKGIIK_11440 0.87 +1.1 2,457,273 - glyA OKGIIK_11440 0.87 -2.4 2,457,273 - glyA OKGIIK_11440 0.87 -1.2 2,457,273 - glyA OKGIIK_11440 0.87 -0.9 2,457,273 - glyA OKGIIK_11440 0.87 +0.4 2,457,273 - glyA OKGIIK_11440 0.87 -0.2 2,457,273 - glyA OKGIIK_11440 0.87 -1.2 2,457,273 - glyA OKGIIK_11440 0.87 -0.4 2,457,323 + +0.1 2,457,323 + -1.5 2,457,323 + -0.2 2,457,323 + -0.9 2,457,323 + -0.1 2,457,323 + -0.9 2,457,323 + +0.8 2,457,324 - +0.1 2,457,324 - -2.7 2,457,324 - -0.8 2,457,324 - +0.4 2,457,324 - -2.3 2,457,324 - +0.3 2,457,324 - -1.8 2,457,324 - +1.1 2,457,324 - -0.2 2,457,405 - -2.1 2,457,550 + -0.4 2,457,550 + -1.0 2,457,550 + +1.9 2,457,550 + -0.9 2,457,551 - -0.0 2,457,551 - -1.5 2,457,551 - +0.7 2,457,552 + -1.0 2,457,553 - -0.7 2,457,590 + -2.1 2,457,590 + -1.0 2,457,590 + +0.1 2,457,591 - -0.4 2,457,591 - -0.4 2,457,591 - -1.3 2,457,638 + -0.2 2,457,638 + -2.1 2,457,639 - -0.0 2,457,639 - -0.8 2,457,644 + -1.0 2,457,644 + -0.5 2,457,644 + -0.2 2,457,644 + -0.2 2,457,644 + -1.4 2,457,644 + -1.2 2,457,644 + +0.3 2,457,644 + +0.3 2,457,644 + +0.2 2,457,645 - -0.2 2,457,645 - -1.0 2,457,645 - -1.5 2,457,846 - ettA OKGIIK_11445 0.13 +0.5 2,457,885 + ettA OKGIIK_11445 0.16 -0.7 2,457,886 - ettA OKGIIK_11445 0.16 -1.6 2,457,950 + ettA OKGIIK_11445 0.19 -1.0 2,457,959 + ettA OKGIIK_11445 0.20 -1.0 2,458,155 + ettA OKGIIK_11445 0.32 -1.6 2,458,155 + ettA OKGIIK_11445 0.32 -0.7 2,458,156 - ettA OKGIIK_11445 0.32 -1.7 2,458,286 + ettA OKGIIK_11445 0.40 +1.0 2,458,358 + ettA OKGIIK_11445 0.44 -1.5 2,458,358 + ettA OKGIIK_11445 0.44 -0.9 2,458,358 + ettA OKGIIK_11445 0.44 -2.0 2,458,358 + ettA OKGIIK_11445 0.44 -2.2 2,458,358 + ettA OKGIIK_11445 0.44 -1.5 2,458,358 + ettA OKGIIK_11445 0.44 -1.0 2,458,358 + ettA OKGIIK_11445 0.44 -0.2 2,458,359 - ettA OKGIIK_11445 0.44 -1.1 2,458,359 - ettA OKGIIK_11445 0.44 +0.0
Or see this region's nucleotide sequence