Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nusB and ribE overlap by 1 nucleotides ribE and ldcA are separated by 220 nucleotides ldcA and ribB are separated by 177 nucleotides
OKGIIK_11405: nusB - transcription antitermination factor NusB, at 2,449,821 to 2,450,267
nusB
OKGIIK_11410: ribE - 6,7-dimethyl-8-ribityllumazine synthase, at 2,450,267 to 2,450,728
ribE
OKGIIK_11415: ldcA - muramoyltetrapeptide carboxypeptidase, at 2,450,949 to 2,451,893
ldcA
OKGIIK_11420: ribB - bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II, at 2,452,071 to 2,453,162
ribB
Position (kb)
2450
2451
2452 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2450.875 kb on + strand at 2450.875 kb on + strand at 2450.875 kb on + strand at 2450.887 kb on + strand at 2450.887 kb on + strand at 2450.887 kb on + strand at 2450.888 kb on - strand at 2450.888 kb on - strand at 2450.888 kb on - strand at 2451.137 kb on - strand, within ldcA at 2451.137 kb on - strand, within ldcA at 2451.137 kb on - strand, within ldcA at 2451.137 kb on - strand, within ldcA at 2451.137 kb on - strand, within ldcA at 2451.229 kb on + strand, within ldcA at 2451.229 kb on + strand, within ldcA at 2451.230 kb on - strand, within ldcA at 2451.230 kb on - strand, within ldcA at 2451.230 kb on - strand, within ldcA at 2451.322 kb on + strand, within ldcA at 2451.322 kb on + strand, within ldcA at 2451.322 kb on + strand, within ldcA at 2451.322 kb on + strand, within ldcA at 2451.323 kb on - strand, within ldcA at 2451.323 kb on - strand, within ldcA at 2451.323 kb on - strand, within ldcA at 2451.323 kb on - strand, within ldcA at 2451.625 kb on + strand, within ldcA at 2451.625 kb on + strand, within ldcA at 2451.626 kb on - strand, within ldcA at 2451.626 kb on - strand, within ldcA at 2451.626 kb on - strand, within ldcA at 2451.626 kb on - strand, within ldcA at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.923 kb on - strand at 2451.985 kb on - strand at 2452.030 kb on + strand at 2452.030 kb on + strand at 2452.031 kb on - strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.078 kb on + strand at 2452.079 kb on - strand at 2452.391 kb on + strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.392 kb on - strand, within ribB at 2452.457 kb on - strand, within ribB at 2452.457 kb on - strand, within ribB at 2452.457 kb on - strand, within ribB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 2,450,875 + -0.0 2,450,875 + +1.8 2,450,875 + +0.3 2,450,887 + -1.3 2,450,887 + -2.6 2,450,887 + +0.7 2,450,888 - -1.2 2,450,888 - -1.4 2,450,888 - -0.4 2,451,137 - ldcA OKGIIK_11415 0.20 +1.3 2,451,137 - ldcA OKGIIK_11415 0.20 -1.0 2,451,137 - ldcA OKGIIK_11415 0.20 -0.2 2,451,137 - ldcA OKGIIK_11415 0.20 -1.6 2,451,137 - ldcA OKGIIK_11415 0.20 +0.7 2,451,229 + ldcA OKGIIK_11415 0.30 -0.7 2,451,229 + ldcA OKGIIK_11415 0.30 -0.7 2,451,230 - ldcA OKGIIK_11415 0.30 +0.1 2,451,230 - ldcA OKGIIK_11415 0.30 -0.9 2,451,230 - ldcA OKGIIK_11415 0.30 -0.7 2,451,322 + ldcA OKGIIK_11415 0.39 -0.2 2,451,322 + ldcA OKGIIK_11415 0.39 -1.6 2,451,322 + ldcA OKGIIK_11415 0.39 -1.2 2,451,322 + ldcA OKGIIK_11415 0.39 +0.1 2,451,323 - ldcA OKGIIK_11415 0.40 -0.2 2,451,323 - ldcA OKGIIK_11415 0.40 -1.0 2,451,323 - ldcA OKGIIK_11415 0.40 -1.2 2,451,323 - ldcA OKGIIK_11415 0.40 +1.1 2,451,625 + ldcA OKGIIK_11415 0.72 +0.1 2,451,625 + ldcA OKGIIK_11415 0.72 -1.4 2,451,626 - ldcA OKGIIK_11415 0.72 -0.4 2,451,626 - ldcA OKGIIK_11415 0.72 -2.6 2,451,626 - ldcA OKGIIK_11415 0.72 +0.1 2,451,626 - ldcA OKGIIK_11415 0.72 -0.7 2,451,923 - -0.6 2,451,923 - -0.4 2,451,923 - -1.0 2,451,923 - +0.7 2,451,923 - -0.4 2,451,985 - -1.4 2,452,030 + -0.5 2,452,030 + -0.2 2,452,031 - +0.1 2,452,078 + -1.0 2,452,078 + -1.3 2,452,078 + -0.9 2,452,078 + -0.7 2,452,078 + -0.7 2,452,078 + +0.7 2,452,078 + -0.2 2,452,079 - -1.0 2,452,391 + ribB OKGIIK_11420 0.29 -0.2 2,452,392 - ribB OKGIIK_11420 0.29 +1.6 2,452,392 - ribB OKGIIK_11420 0.29 -0.8 2,452,392 - ribB OKGIIK_11420 0.29 -1.0 2,452,392 - ribB OKGIIK_11420 0.29 +1.3 2,452,392 - ribB OKGIIK_11420 0.29 -1.3 2,452,392 - ribB OKGIIK_11420 0.29 -0.2 2,452,392 - ribB OKGIIK_11420 0.29 -1.0 2,452,392 - ribB OKGIIK_11420 0.29 +0.2 2,452,392 - ribB OKGIIK_11420 0.29 -0.2 2,452,392 - ribB OKGIIK_11420 0.29 +0.1 2,452,457 - ribB OKGIIK_11420 0.35 -0.0 2,452,457 - ribB OKGIIK_11420 0.35 -0.2 2,452,457 - ribB OKGIIK_11420 0.35 -0.2
Or see this region's nucleotide sequence