Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaG and pgpA are separated by 20 nucleotides pgpA and thiL overlap by 4 nucleotides thiL and OKGIIK_11400 are separated by 21 nucleotides
OKGIIK_11385: wcaG - NAD dependent epimerase/dehydratase family, at 2,446,779 to 2,447,762
wcaG
OKGIIK_11390: pgpA - Phosphatidylglycerophosphatase A, at 2,447,783 to 2,448,295
pgpA
OKGIIK_11395: thiL - thiamine-phosphate kinase, at 2,448,292 to 2,449,257
thiL
OKGIIK_11400: OKGIIK_11400 - Acetyltransferase, at 2,449,279 to 2,449,821
_11400
Position (kb)
2447
2448
2449 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2446.804 kb on + strand at 2446.805 kb on - strand at 2446.805 kb on - strand at 2446.870 kb on + strand at 2446.870 kb on + strand at 2446.871 kb on - strand at 2447.109 kb on + strand, within wcaG at 2447.109 kb on + strand, within wcaG at 2447.109 kb on + strand, within wcaG at 2447.115 kb on + strand, within wcaG at 2447.115 kb on + strand, within wcaG at 2447.115 kb on + strand, within wcaG at 2447.115 kb on + strand, within wcaG at 2447.115 kb on + strand, within wcaG at 2447.115 kb on + strand, within wcaG at 2447.115 kb on + strand, within wcaG at 2447.337 kb on + strand, within wcaG at 2447.338 kb on - strand, within wcaG at 2447.338 kb on - strand, within wcaG at 2447.373 kb on + strand, within wcaG at 2447.373 kb on + strand, within wcaG at 2447.373 kb on + strand, within wcaG at 2447.374 kb on - strand, within wcaG at 2447.374 kb on - strand, within wcaG at 2447.374 kb on - strand, within wcaG at 2447.374 kb on - strand, within wcaG at 2447.604 kb on + strand, within wcaG at 2447.604 kb on + strand, within wcaG at 2447.604 kb on + strand, within wcaG at 2447.604 kb on + strand, within wcaG at 2447.604 kb on + strand, within wcaG at 2447.605 kb on - strand, within wcaG at 2447.605 kb on - strand, within wcaG at 2447.605 kb on - strand, within wcaG at 2447.605 kb on - strand, within wcaG at 2447.763 kb on + strand at 2447.763 kb on + strand at 2447.763 kb on + strand at 2447.763 kb on + strand at 2447.763 kb on + strand at 2447.763 kb on + strand at 2447.764 kb on - strand at 2447.764 kb on - strand at 2447.764 kb on - strand at 2447.764 kb on - strand at 2447.764 kb on - strand at 2447.764 kb on - strand at 2447.764 kb on - strand at 2447.764 kb on - strand at 2447.764 kb on - strand at 2447.784 kb on + strand at 2447.784 kb on + strand at 2447.784 kb on + strand at 2447.784 kb on + strand at 2447.784 kb on + strand at 2447.784 kb on + strand at 2447.784 kb on + strand at 2447.784 kb on + strand at 2447.785 kb on - strand at 2447.785 kb on - strand at 2447.785 kb on - strand at 2447.785 kb on - strand at 2447.785 kb on - strand at 2447.785 kb on - strand at 2447.785 kb on - strand at 2447.900 kb on + strand, within pgpA at 2447.901 kb on - strand, within pgpA at 2447.901 kb on - strand, within pgpA at 2447.901 kb on - strand, within pgpA at 2447.901 kb on - strand, within pgpA at 2448.121 kb on - strand, within pgpA at 2448.121 kb on - strand, within pgpA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 2,446,804 + -0.2 2,446,805 - +0.7 2,446,805 - +0.9 2,446,870 + -1.4 2,446,870 + +0.1 2,446,871 - -0.0 2,447,109 + wcaG OKGIIK_11385 0.34 -1.6 2,447,109 + wcaG OKGIIK_11385 0.34 -1.5 2,447,109 + wcaG OKGIIK_11385 0.34 +0.6 2,447,115 + wcaG OKGIIK_11385 0.34 -0.1 2,447,115 + wcaG OKGIIK_11385 0.34 +0.6 2,447,115 + wcaG OKGIIK_11385 0.34 -1.1 2,447,115 + wcaG OKGIIK_11385 0.34 +1.1 2,447,115 + wcaG OKGIIK_11385 0.34 +0.7 2,447,115 + wcaG OKGIIK_11385 0.34 -0.6 2,447,115 + wcaG OKGIIK_11385 0.34 -0.2 2,447,337 + wcaG OKGIIK_11385 0.57 +1.1 2,447,338 - wcaG OKGIIK_11385 0.57 -1.4 2,447,338 - wcaG OKGIIK_11385 0.57 -1.4 2,447,373 + wcaG OKGIIK_11385 0.60 +0.0 2,447,373 + wcaG OKGIIK_11385 0.60 -1.2 2,447,373 + wcaG OKGIIK_11385 0.60 -0.2 2,447,374 - wcaG OKGIIK_11385 0.60 +1.1 2,447,374 - wcaG OKGIIK_11385 0.60 -0.2 2,447,374 - wcaG OKGIIK_11385 0.60 -0.7 2,447,374 - wcaG OKGIIK_11385 0.60 +0.9 2,447,604 + wcaG OKGIIK_11385 0.84 -1.4 2,447,604 + wcaG OKGIIK_11385 0.84 +0.1 2,447,604 + wcaG OKGIIK_11385 0.84 -0.7 2,447,604 + wcaG OKGIIK_11385 0.84 -0.4 2,447,604 + wcaG OKGIIK_11385 0.84 +0.1 2,447,605 - wcaG OKGIIK_11385 0.84 +2.6 2,447,605 - wcaG OKGIIK_11385 0.84 +0.3 2,447,605 - wcaG OKGIIK_11385 0.84 -0.7 2,447,605 - wcaG OKGIIK_11385 0.84 -1.0 2,447,763 + +0.8 2,447,763 + -0.9 2,447,763 + -0.7 2,447,763 + +1.4 2,447,763 + -1.9 2,447,763 + -1.8 2,447,764 - -1.4 2,447,764 - -0.7 2,447,764 - +1.1 2,447,764 - -0.9 2,447,764 - +0.1 2,447,764 - -0.1 2,447,764 - +0.3 2,447,764 - -0.4 2,447,764 - -1.3 2,447,784 + +0.1 2,447,784 + +0.8 2,447,784 + +0.4 2,447,784 + -0.2 2,447,784 + +0.8 2,447,784 + +1.4 2,447,784 + +0.2 2,447,784 + +1.4 2,447,785 - -0.0 2,447,785 - +0.3 2,447,785 - +0.1 2,447,785 - +0.1 2,447,785 - +2.1 2,447,785 - +0.1 2,447,785 - -0.3 2,447,900 + pgpA OKGIIK_11390 0.23 +1.1 2,447,901 - pgpA OKGIIK_11390 0.23 -0.9 2,447,901 - pgpA OKGIIK_11390 0.23 -1.9 2,447,901 - pgpA OKGIIK_11390 0.23 -1.4 2,447,901 - pgpA OKGIIK_11390 0.23 -0.4 2,448,121 - pgpA OKGIIK_11390 0.66 -2.1 2,448,121 - pgpA OKGIIK_11390 0.66 -0.2
Or see this region's nucleotide sequence