Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysQ and nudE overlap by 4 nucleotides nudE and suhB are separated by 23 nucleotides suhB and OKGIIK_11010 are separated by 15 nucleotides OKGIIK_11010 and bioA are separated by 70 nucleotides
OKGIIK_10995: cysQ - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 2,354,293 to 2,355,114
cysQ
OKGIIK_11000: nudE - ADP compounds hydrolase NudE, at 2,355,111 to 2,355,668
nudE
OKGIIK_11005: suhB - Archaeal fructose-1,6-bisphosphatase or related enzyme, inositol monophosphatase family, at 2,355,692 to 2,356,468
suhB
OKGIIK_11010: OKGIIK_11010 - Type II secretion system protein N, at 2,356,484 to 2,357,269
_11010
OKGIIK_11015: bioA - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 2,357,340 to 2,358,731
bioA
Position (kb)
2355
2356
2357 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.864 kb on + strand, within cysQ at 2354.864 kb on + strand, within cysQ at 2354.864 kb on + strand, within cysQ at 2354.865 kb on - strand, within cysQ at 2355.038 kb on + strand at 2355.038 kb on + strand at 2355.038 kb on + strand at 2355.039 kb on - strand at 2355.039 kb on - strand at 2355.039 kb on - strand at 2355.039 kb on - strand at 2355.039 kb on - strand at 2355.039 kb on - strand at 2355.139 kb on + strand at 2355.139 kb on + strand at 2355.139 kb on + strand at 2355.140 kb on - strand at 2355.140 kb on - strand at 2355.140 kb on - strand at 2355.140 kb on - strand at 2355.277 kb on + strand, within nudE at 2355.277 kb on + strand, within nudE at 2355.278 kb on - strand, within nudE at 2355.278 kb on - strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.457 kb on + strand, within nudE at 2355.457 kb on + strand, within nudE at 2355.478 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.658 kb on - strand at 2355.658 kb on - strand at 2355.658 kb on - strand at 2355.658 kb on - strand at 2355.679 kb on + strand at 2355.679 kb on + strand at 2355.679 kb on + strand at 2355.679 kb on + strand at 2355.679 kb on + strand at 2355.680 kb on - strand at 2355.680 kb on - strand at 2355.680 kb on - strand at 2355.680 kb on - strand at 2355.680 kb on - strand at 2355.897 kb on + strand, within suhB at 2355.897 kb on + strand, within suhB at 2355.897 kb on + strand, within suhB at 2356.101 kb on + strand, within suhB at 2356.101 kb on + strand, within suhB at 2356.102 kb on - strand, within suhB at 2356.102 kb on - strand, within suhB at 2356.267 kb on - strand, within suhB at 2356.390 kb on - strand, within suhB at 2356.775 kb on - strand, within OKGIIK_11010 at 2356.899 kb on + strand, within OKGIIK_11010 at 2356.899 kb on + strand, within OKGIIK_11010 at 2356.899 kb on + strand, within OKGIIK_11010 at 2356.899 kb on + strand, within OKGIIK_11010 at 2356.900 kb on - strand, within OKGIIK_11010 at 2356.900 kb on - strand, within OKGIIK_11010 at 2357.333 kb on + strand at 2357.333 kb on + strand at 2357.334 kb on - strand at 2357.334 kb on - strand at 2357.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 2,354,729 + cysQ OKGIIK_10995 0.53 +0.9 2,354,729 + cysQ OKGIIK_10995 0.53 -1.1 2,354,729 + cysQ OKGIIK_10995 0.53 -1.3 2,354,729 + cysQ OKGIIK_10995 0.53 -0.4 2,354,729 + cysQ OKGIIK_10995 0.53 +0.4 2,354,729 + cysQ OKGIIK_10995 0.53 -1.1 2,354,729 + cysQ OKGIIK_10995 0.53 +1.9 2,354,730 - cysQ OKGIIK_10995 0.53 +0.7 2,354,730 - cysQ OKGIIK_10995 0.53 +0.9 2,354,730 - cysQ OKGIIK_10995 0.53 -0.0 2,354,730 - cysQ OKGIIK_10995 0.53 +0.4 2,354,730 - cysQ OKGIIK_10995 0.53 -1.0 2,354,730 - cysQ OKGIIK_10995 0.53 -1.1 2,354,864 + cysQ OKGIIK_10995 0.69 -0.9 2,354,864 + cysQ OKGIIK_10995 0.69 -0.5 2,354,864 + cysQ OKGIIK_10995 0.69 -0.6 2,354,865 - cysQ OKGIIK_10995 0.70 -0.1 2,355,038 + -0.3 2,355,038 + +0.2 2,355,038 + +0.1 2,355,039 - -1.3 2,355,039 - -1.1 2,355,039 - -1.1 2,355,039 - +0.5 2,355,039 - -0.1 2,355,039 - -0.2 2,355,139 + +0.6 2,355,139 + -0.1 2,355,139 + -1.1 2,355,140 - +1.0 2,355,140 - +1.2 2,355,140 - +0.4 2,355,140 - -2.0 2,355,277 + nudE OKGIIK_11000 0.30 -0.1 2,355,277 + nudE OKGIIK_11000 0.30 -2.1 2,355,278 - nudE OKGIIK_11000 0.30 -0.7 2,355,278 - nudE OKGIIK_11000 0.30 -0.6 2,355,319 + nudE OKGIIK_11000 0.37 -1.5 2,355,319 + nudE OKGIIK_11000 0.37 +1.4 2,355,319 + nudE OKGIIK_11000 0.37 -0.4 2,355,319 + nudE OKGIIK_11000 0.37 -0.4 2,355,319 + nudE OKGIIK_11000 0.37 +0.4 2,355,319 + nudE OKGIIK_11000 0.37 -0.8 2,355,319 + nudE OKGIIK_11000 0.37 -1.0 2,355,319 + nudE OKGIIK_11000 0.37 -0.3 2,355,319 + nudE OKGIIK_11000 0.37 +0.0 2,355,319 + nudE OKGIIK_11000 0.37 -0.3 2,355,319 + nudE OKGIIK_11000 0.37 +0.2 2,355,319 + nudE OKGIIK_11000 0.37 -1.6 2,355,319 + nudE OKGIIK_11000 0.37 +0.4 2,355,319 + nudE OKGIIK_11000 0.37 -2.6 2,355,319 + nudE OKGIIK_11000 0.37 -0.7 2,355,319 + nudE OKGIIK_11000 0.37 +0.7 2,355,319 + nudE OKGIIK_11000 0.37 +1.6 2,355,319 + nudE OKGIIK_11000 0.37 +0.3 2,355,319 + nudE OKGIIK_11000 0.37 -0.1 2,355,319 + nudE OKGIIK_11000 0.37 +0.0 2,355,319 + nudE OKGIIK_11000 0.37 +0.1 2,355,320 - nudE OKGIIK_11000 0.37 +2.2 2,355,320 - nudE OKGIIK_11000 0.37 +0.7 2,355,320 - nudE OKGIIK_11000 0.37 -0.6 2,355,320 - nudE OKGIIK_11000 0.37 +0.4 2,355,320 - nudE OKGIIK_11000 0.37 +0.2 2,355,320 - nudE OKGIIK_11000 0.37 -2.6 2,355,320 - nudE OKGIIK_11000 0.37 -0.6 2,355,320 - nudE OKGIIK_11000 0.37 +1.2 2,355,320 - nudE OKGIIK_11000 0.37 +0.1 2,355,320 - nudE OKGIIK_11000 0.37 +0.2 2,355,320 - nudE OKGIIK_11000 0.37 +1.8 2,355,320 - nudE OKGIIK_11000 0.37 +0.4 2,355,320 - nudE OKGIIK_11000 0.37 -1.4 2,355,320 - nudE OKGIIK_11000 0.37 +0.9 2,355,320 - nudE OKGIIK_11000 0.37 +0.2 2,355,320 - nudE OKGIIK_11000 0.37 -0.8 2,355,320 - nudE OKGIIK_11000 0.37 +0.8 2,355,320 - nudE OKGIIK_11000 0.37 -1.7 2,355,457 + nudE OKGIIK_11000 0.62 -0.2 2,355,457 + nudE OKGIIK_11000 0.62 -0.1 2,355,478 + nudE OKGIIK_11000 0.66 -0.3 2,355,562 + nudE OKGIIK_11000 0.81 +0.9 2,355,562 + nudE OKGIIK_11000 0.81 -1.8 2,355,562 + nudE OKGIIK_11000 0.81 +1.0 2,355,562 + nudE OKGIIK_11000 0.81 -1.1 2,355,562 + nudE OKGIIK_11000 0.81 -1.0 2,355,563 - nudE OKGIIK_11000 0.81 -0.2 2,355,563 - nudE OKGIIK_11000 0.81 -1.0 2,355,563 - nudE OKGIIK_11000 0.81 +0.2 2,355,563 - nudE OKGIIK_11000 0.81 +0.8 2,355,563 - nudE OKGIIK_11000 0.81 -1.5 2,355,563 - nudE OKGIIK_11000 0.81 -0.6 2,355,658 - -1.0 2,355,658 - -1.5 2,355,658 - -3.0 2,355,658 - -0.8 2,355,679 + +1.2 2,355,679 + -0.1 2,355,679 + -0.5 2,355,679 + -1.3 2,355,679 + -0.1 2,355,680 - -0.1 2,355,680 - +0.1 2,355,680 - +1.0 2,355,680 - +0.5 2,355,680 - +0.7 2,355,897 + suhB OKGIIK_11005 0.26 -2.3 2,355,897 + suhB OKGIIK_11005 0.26 -0.6 2,355,897 + suhB OKGIIK_11005 0.26 +0.2 2,356,101 + suhB OKGIIK_11005 0.53 -0.6 2,356,101 + suhB OKGIIK_11005 0.53 -1.3 2,356,102 - suhB OKGIIK_11005 0.53 -0.1 2,356,102 - suhB OKGIIK_11005 0.53 +0.2 2,356,267 - suhB OKGIIK_11005 0.74 -0.7 2,356,390 - suhB OKGIIK_11005 0.90 -1.4 2,356,775 - OKGIIK_11010 0.37 +2.0 2,356,899 + OKGIIK_11010 0.53 -0.8 2,356,899 + OKGIIK_11010 0.53 +0.4 2,356,899 + OKGIIK_11010 0.53 -0.1 2,356,899 + OKGIIK_11010 0.53 -1.3 2,356,900 - OKGIIK_11010 0.53 -0.1 2,356,900 - OKGIIK_11010 0.53 +0.1 2,357,333 + +0.2 2,357,333 + -0.8 2,357,334 - -0.5 2,357,334 - -0.6 2,357,334 - -0.3
Or see this region's nucleotide sequence