Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_08910 and OKGIIK_08915 are separated by 25 nucleotides OKGIIK_08915 and OKGIIK_08920 overlap by 4 nucleotides OKGIIK_08920 and OKGIIK_08925 overlap by 8 nucleotides OKGIIK_08925 and OKGIIK_08930 overlap by 4 nucleotides OKGIIK_08930 and OKGIIK_08935 overlap by 8 nucleotides OKGIIK_08935 and OKGIIK_08940 overlap by 4 nucleotides OKGIIK_08940 and OKGIIK_08945 are separated by 2 nucleotides
OKGIIK_08910: OKGIIK_08910 - hypothetical protein, at 1,919,007 to 1,919,471
_08910
OKGIIK_08915: OKGIIK_08915 - hypothetical protein, at 1,919,497 to 1,919,757
_08915
OKGIIK_08920: OKGIIK_08920 - hypothetical protein, at 1,919,754 to 1,919,972
_08920
OKGIIK_08925: OKGIIK_08925 - hypothetical protein, at 1,919,965 to 1,920,363
_08925
OKGIIK_08930: OKGIIK_08930 - hypothetical protein, at 1,920,360 to 1,920,629
_08930
OKGIIK_08935: OKGIIK_08935 - hypothetical protein, at 1,920,622 to 1,920,969
_08935
OKGIIK_08940: OKGIIK_08940 - DUF4224 domain-containing protein, at 1,920,966 to 1,921,169
_08940
OKGIIK_08945: OKGIIK_08945 - Integrase, at 1,921,172 to 1,922,200
_08945
Position (kb)
1920
1921 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1919.389 kb on - strand, within OKGIIK_08910 at 1919.662 kb on + strand, within OKGIIK_08915 at 1919.663 kb on - strand, within OKGIIK_08915 at 1919.898 kb on + strand, within OKGIIK_08920 at 1919.899 kb on - strand, within OKGIIK_08920 at 1919.899 kb on - strand, within OKGIIK_08920 at 1919.899 kb on - strand, within OKGIIK_08920 at 1919.924 kb on - strand, within OKGIIK_08920 at 1919.974 kb on + strand at 1919.974 kb on + strand at 1919.974 kb on + strand at 1919.974 kb on + strand at 1919.974 kb on + strand at 1919.974 kb on + strand at 1919.975 kb on - strand at 1919.975 kb on - strand at 1919.998 kb on - strand at 1920.041 kb on + strand, within OKGIIK_08925 at 1920.041 kb on + strand, within OKGIIK_08925 at 1920.041 kb on + strand, within OKGIIK_08925 at 1920.042 kb on - strand, within OKGIIK_08925 at 1920.042 kb on - strand, within OKGIIK_08925 at 1920.042 kb on - strand, within OKGIIK_08925 at 1920.042 kb on - strand, within OKGIIK_08925 at 1920.042 kb on - strand, within OKGIIK_08925 at 1920.042 kb on - strand, within OKGIIK_08925 at 1920.042 kb on - strand, within OKGIIK_08925 at 1920.042 kb on - strand, within OKGIIK_08925 at 1920.042 kb on - strand, within OKGIIK_08925 at 1920.099 kb on + strand, within OKGIIK_08925 at 1920.099 kb on + strand, within OKGIIK_08925 at 1920.100 kb on - strand, within OKGIIK_08925 at 1920.100 kb on - strand, within OKGIIK_08925 at 1920.100 kb on - strand, within OKGIIK_08925 at 1920.126 kb on + strand, within OKGIIK_08925 at 1920.127 kb on - strand, within OKGIIK_08925 at 1920.143 kb on + strand, within OKGIIK_08925 at 1920.143 kb on + strand, within OKGIIK_08925 at 1920.143 kb on + strand, within OKGIIK_08925 at 1920.143 kb on + strand, within OKGIIK_08925 at 1920.143 kb on + strand, within OKGIIK_08925 at 1920.143 kb on + strand, within OKGIIK_08925 at 1920.144 kb on - strand, within OKGIIK_08925 at 1920.144 kb on - strand, within OKGIIK_08925 at 1920.148 kb on + strand, within OKGIIK_08925 at 1920.149 kb on - strand, within OKGIIK_08925 at 1920.149 kb on - strand, within OKGIIK_08925 at 1920.149 kb on - strand, within OKGIIK_08925 at 1920.171 kb on + strand, within OKGIIK_08925 at 1920.171 kb on + strand, within OKGIIK_08925 at 1920.171 kb on + strand, within OKGIIK_08925 at 1920.171 kb on + strand, within OKGIIK_08925 at 1920.430 kb on - strand, within OKGIIK_08930 at 1920.454 kb on + strand, within OKGIIK_08930 at 1920.454 kb on + strand, within OKGIIK_08930 at 1920.454 kb on + strand, within OKGIIK_08930 at 1920.454 kb on + strand, within OKGIIK_08930 at 1920.454 kb on + strand, within OKGIIK_08930 at 1920.454 kb on + strand, within OKGIIK_08930 at 1920.454 kb on + strand, within OKGIIK_08930 at 1920.454 kb on + strand, within OKGIIK_08930 at 1920.454 kb on + strand, within OKGIIK_08930 at 1920.454 kb on + strand, within OKGIIK_08930 at 1920.455 kb on - strand, within OKGIIK_08930 at 1920.455 kb on - strand, within OKGIIK_08930 at 1920.571 kb on + strand, within OKGIIK_08930 at 1920.571 kb on + strand, within OKGIIK_08930 at 1920.572 kb on - strand, within OKGIIK_08930 at 1920.572 kb on - strand, within OKGIIK_08930 at 1920.628 kb on - strand at 1920.854 kb on + strand, within OKGIIK_08935 at 1920.854 kb on + strand, within OKGIIK_08935 at 1920.854 kb on + strand, within OKGIIK_08935 at 1920.855 kb on - strand, within OKGIIK_08935 at 1920.855 kb on - strand, within OKGIIK_08935 at 1920.855 kb on - strand, within OKGIIK_08935 at 1920.855 kb on - strand, within OKGIIK_08935 at 1920.858 kb on + strand, within OKGIIK_08935 at 1920.946 kb on + strand at 1920.973 kb on + strand at 1921.065 kb on + strand, within OKGIIK_08940 at 1921.065 kb on + strand, within OKGIIK_08940 at 1921.065 kb on + strand, within OKGIIK_08940 at 1921.066 kb on - strand, within OKGIIK_08940 at 1921.247 kb on + strand at 1921.247 kb on + strand at 1921.247 kb on + strand at 1921.247 kb on + strand at 1921.247 kb on + strand at 1921.248 kb on - strand at 1921.248 kb on - strand at 1921.248 kb on - strand at 1921.248 kb on - strand at 1921.248 kb on - strand at 1921.248 kb on - strand at 1921.248 kb on - strand at 1921.250 kb on + strand at 1921.250 kb on + strand at 1921.251 kb on - strand at 1921.251 kb on - strand at 1921.253 kb on + strand at 1921.253 kb on + strand at 1921.253 kb on + strand at 1921.253 kb on + strand at 1921.254 kb on - strand at 1921.254 kb on - strand at 1921.254 kb on - strand at 1921.301 kb on + strand, within OKGIIK_08945 at 1921.302 kb on - strand, within OKGIIK_08945 at 1921.395 kb on - strand, within OKGIIK_08945 at 1921.529 kb on + strand, within OKGIIK_08945 at 1921.530 kb on - strand, within OKGIIK_08945 at 1921.530 kb on - strand, within OKGIIK_08945 at 1921.530 kb on - strand, within OKGIIK_08945 at 1921.533 kb on + strand, within OKGIIK_08945 at 1921.533 kb on + strand, within OKGIIK_08945 at 1921.534 kb on - strand, within OKGIIK_08945 at 1921.534 kb on - strand, within OKGIIK_08945 at 1921.534 kb on - strand, within OKGIIK_08945 at 1921.622 kb on + strand, within OKGIIK_08945 at 1921.622 kb on + strand, within OKGIIK_08945 at 1921.622 kb on + strand, within OKGIIK_08945 at 1921.623 kb on - strand, within OKGIIK_08945 at 1921.623 kb on - strand, within OKGIIK_08945 at 1921.623 kb on - strand, within OKGIIK_08945
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 1,919,389 - OKGIIK_08910 0.82 +1.6 1,919,662 + OKGIIK_08915 0.63 +0.5 1,919,663 - OKGIIK_08915 0.64 -1.5 1,919,898 + OKGIIK_08920 0.66 -0.4 1,919,899 - OKGIIK_08920 0.66 +2.2 1,919,899 - OKGIIK_08920 0.66 +0.4 1,919,899 - OKGIIK_08920 0.66 -0.1 1,919,924 - OKGIIK_08920 0.78 -0.1 1,919,974 + -0.4 1,919,974 + -1.0 1,919,974 + +0.4 1,919,974 + -0.6 1,919,974 + -1.0 1,919,974 + +0.5 1,919,975 - -0.6 1,919,975 - -0.8 1,919,998 - -0.5 1,920,041 + OKGIIK_08925 0.19 -0.1 1,920,041 + OKGIIK_08925 0.19 +0.6 1,920,041 + OKGIIK_08925 0.19 -0.8 1,920,042 - OKGIIK_08925 0.19 -0.8 1,920,042 - OKGIIK_08925 0.19 -0.1 1,920,042 - OKGIIK_08925 0.19 -0.6 1,920,042 - OKGIIK_08925 0.19 +0.8 1,920,042 - OKGIIK_08925 0.19 -0.2 1,920,042 - OKGIIK_08925 0.19 +2.2 1,920,042 - OKGIIK_08925 0.19 -0.7 1,920,042 - OKGIIK_08925 0.19 -1.0 1,920,042 - OKGIIK_08925 0.19 -0.9 1,920,099 + OKGIIK_08925 0.34 -0.1 1,920,099 + OKGIIK_08925 0.34 +0.5 1,920,100 - OKGIIK_08925 0.34 +0.7 1,920,100 - OKGIIK_08925 0.34 +0.6 1,920,100 - OKGIIK_08925 0.34 +0.6 1,920,126 + OKGIIK_08925 0.40 -0.1 1,920,127 - OKGIIK_08925 0.41 +1.5 1,920,143 + OKGIIK_08925 0.45 -1.3 1,920,143 + OKGIIK_08925 0.45 +0.0 1,920,143 + OKGIIK_08925 0.45 -0.9 1,920,143 + OKGIIK_08925 0.45 -0.1 1,920,143 + OKGIIK_08925 0.45 -2.1 1,920,143 + OKGIIK_08925 0.45 -0.5 1,920,144 - OKGIIK_08925 0.45 +0.5 1,920,144 - OKGIIK_08925 0.45 -0.8 1,920,148 + OKGIIK_08925 0.46 +0.8 1,920,149 - OKGIIK_08925 0.46 +0.4 1,920,149 - OKGIIK_08925 0.46 -1.4 1,920,149 - OKGIIK_08925 0.46 -0.0 1,920,171 + OKGIIK_08925 0.52 +0.4 1,920,171 + OKGIIK_08925 0.52 +0.6 1,920,171 + OKGIIK_08925 0.52 -1.2 1,920,171 + OKGIIK_08925 0.52 -0.1 1,920,430 - OKGIIK_08930 0.26 -0.6 1,920,454 + OKGIIK_08930 0.35 +0.6 1,920,454 + OKGIIK_08930 0.35 -0.3 1,920,454 + OKGIIK_08930 0.35 -2.4 1,920,454 + OKGIIK_08930 0.35 +0.2 1,920,454 + OKGIIK_08930 0.35 +1.2 1,920,454 + OKGIIK_08930 0.35 -0.8 1,920,454 + OKGIIK_08930 0.35 +1.9 1,920,454 + OKGIIK_08930 0.35 +0.2 1,920,454 + OKGIIK_08930 0.35 -1.5 1,920,454 + OKGIIK_08930 0.35 -0.8 1,920,455 - OKGIIK_08930 0.35 -0.7 1,920,455 - OKGIIK_08930 0.35 -0.1 1,920,571 + OKGIIK_08930 0.78 -0.9 1,920,571 + OKGIIK_08930 0.78 -0.1 1,920,572 - OKGIIK_08930 0.79 -1.2 1,920,572 - OKGIIK_08930 0.79 -0.7 1,920,628 - +0.8 1,920,854 + OKGIIK_08935 0.67 +0.7 1,920,854 + OKGIIK_08935 0.67 +0.0 1,920,854 + OKGIIK_08935 0.67 -0.8 1,920,855 - OKGIIK_08935 0.67 +1.4 1,920,855 - OKGIIK_08935 0.67 -1.2 1,920,855 - OKGIIK_08935 0.67 -0.5 1,920,855 - OKGIIK_08935 0.67 +0.9 1,920,858 + OKGIIK_08935 0.68 +1.2 1,920,946 + +0.8 1,920,973 + -0.8 1,921,065 + OKGIIK_08940 0.49 -1.0 1,921,065 + OKGIIK_08940 0.49 +1.2 1,921,065 + OKGIIK_08940 0.49 -0.4 1,921,066 - OKGIIK_08940 0.49 -1.0 1,921,247 + +0.3 1,921,247 + -0.7 1,921,247 + +0.1 1,921,247 + -1.1 1,921,247 + +1.0 1,921,248 - +1.9 1,921,248 - -0.2 1,921,248 - -2.4 1,921,248 - -1.6 1,921,248 - -0.9 1,921,248 - -2.6 1,921,248 - +0.0 1,921,250 + +0.6 1,921,250 + -1.6 1,921,251 - +0.5 1,921,251 - +0.4 1,921,253 + -1.4 1,921,253 + -1.7 1,921,253 + -0.2 1,921,253 + +0.2 1,921,254 - -0.8 1,921,254 - -0.8 1,921,254 - +1.2 1,921,301 + OKGIIK_08945 0.13 +0.8 1,921,302 - OKGIIK_08945 0.13 +1.5 1,921,395 - OKGIIK_08945 0.22 -1.2 1,921,529 + OKGIIK_08945 0.35 -0.1 1,921,530 - OKGIIK_08945 0.35 +0.0 1,921,530 - OKGIIK_08945 0.35 -1.6 1,921,530 - OKGIIK_08945 0.35 -0.6 1,921,533 + OKGIIK_08945 0.35 +0.8 1,921,533 + OKGIIK_08945 0.35 -0.7 1,921,534 - OKGIIK_08945 0.35 +1.4 1,921,534 - OKGIIK_08945 0.35 -0.3 1,921,534 - OKGIIK_08945 0.35 -0.2 1,921,622 + OKGIIK_08945 0.44 -2.2 1,921,622 + OKGIIK_08945 0.44 -0.8 1,921,622 + OKGIIK_08945 0.44 -0.1 1,921,623 - OKGIIK_08945 0.44 -0.2 1,921,623 - OKGIIK_08945 0.44 -0.4 1,921,623 - OKGIIK_08945 0.44 -0.1
Or see this region's nucleotide sequence